GilaA.01452.a
Aldose reductase
| CENTER ID: | GilaA.01452.a |
| ORGANISM: | Giardia lamblia ATCC 50803 |
| ASSOCIATED DISEASE: | Giardiasis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| GilaA.01452.a.A1.GE25746 | Full length( GilaA.01452.a ) | 1 | 313 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| GilaA.01452.a.A1.PS00377 | Full length( GilaA.01452.a ) | 1 | 313 |
Structures
3KRB
DEPOSITED: 11/18/2009
DETERMINATION: XRay
CLONE: GilaA.01452.a.A1.GE25746
PROTEIN: GilaA.01452.a.A1.PS00377
Publications by SSGCID
Structure of aldose reductase from Giardia lamblia.
Abendroth J, Edwards TE, Ferrell M, Myler PJ, Sankaran B, Staker BL, Stewart LJ, Van Voorhis WC, Zhang Y
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2011
volume 67, issue Pt 9, pages 1113-7
PMID: 21904059; PMCID: PMC3169411
PDB:
3KRB
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | GiardiaDB:GL50803_7260 |
| OrthoMCL: | OG5_126583 |
| RefSeq: | XP_001707007.1 |
| UniProt: | A8BGX6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQYPPRLGFG TWQAPPEAVQ TAVETALMTG YRHIDCAYVY QNEEAIGRAF GKIFKDASSG IKREDVWITS KLWNYNHRPE LVREQCKKTM SDLQVDYLDL FLVHWPLAFV RNDVGDLFPK DAEGRAMLEK VPLADTWRAM EQLVEEGLVK HIGVSNYTVP LLADLLNYAK IKPLVNQIEI HPWHPNDATV KFCLDNGIGV TAYSPMGGSY ADPRDPSGTQ KNVILECKTL KAIADAKGTS PHCVALAWHV KKWNTSMYSV IPKSQTPARI EANFKCTEVQ LSDDDMDAIN NIHLNKRIRF CDPAIFWKVP LFD
NT Sequence
ATGCAATATC CTCCTCGCTT GGGCTTTGGA ACGTGGCAGG CGCCTCCAGA GGCCGTGCAG ACCGCTGTTG AGACAGCGCT CATGACTGGG TACAGGCACA TCGACTGCGC CTATGTCTAC CAGAATGAGG AGGCCATTGG TCGTGCTTTT GGAAAGATCT TCAAGGATGC GTCTTCAGGA ATCAAGCGCG AAGATGTCTG GATCACCTCA AAGCTATGGA ACTATAACCA TCGCCCAGAA CTGGTCCGTG AGCAGTGCAA GAAAACCATG TCTGACTTAC AGGTGGATTA CCTAGATCTC TTCCTTGTCC ACTGGCCGCT TGCCTTTGTT CGTAATGACG TCGGGGATCT CTTCCCGAAG GATGCCGAAG GCCGTGCGAT GCTCGAGAAG GTCCCCCTGG CCGACACATG GAGGGCAATG GAACAGCTGG TCGAGGAGGG CCTTGTGAAG CACATCGGGG TCTCGAATTA CACGGTCCCT CTTTTGGCCG ATCTACTAAA CTACGCCAAG ATCAAGCCTC TTGTGAATCA GATCGAGATA CACCCGTGGC ACCCCAACGA CGCTACCGTA AAGTTCTGCC TTGACAACGG AATAGGTGTG ACAGCCTACT CTCCCATGGG TGGATCATAT GCAGATCCCA GGGATCCCTC TGGCACACAG AAGAACGTGA TCCTTGAGTG CAAGACGCTG AAAGCAATAG CCGATGCCAA AGGGACAAGC CCGCACTGTG TGGCGCTCGC TTGGCACGTG AAGAAGTGGA ACACCTCGAT GTACAGTGTC ATTCCTAAGA GCCAGACGCC CGCGCGCATT GAGGCCAACT TCAAGTGCAC CGAAGTACAG CTGAGCGATG ATGACATGGA TGCAATCAAC AATATTCACT TGAACAAGAG AATCCGCTTT TGCGATCCCG CTATCTTTTG GAAGGTACCA CTGTTTGAC
Details for GilaA.01452.a.A1.GE25746
| HARVESTED ON: | 10/31/2008 |
| SEQUENCED ON: | 7/20/2010 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 38kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGP G SMQYPPRL GF GTWQAPP EAV QTAVET ALMT GYRHI DCAYV YQNE EAIGRA FGK IFKDASS GI KREDVWIT S KLWNYNHRP ELVREQCKKT MSDLQVDYL D LFLVHWPL AF VRNDVGD LFP KDAEGR AMLE KVPLA DTWRA MEQL VEEGLV KHI GVSNYTV PL LADLLNYA K IKPLVNQIE IHPWHPNDAT VKFCLDNGI G VTAYSPMG GS YADPRDP SGT QKNVIL ECKT LKAIA DAKGT SPHC VALAWH VKK WNTSMYS VI PKSQTPAR I EANFKCTEV QLSDDDMDAI NNIHLNKRI R FCDPAIFW KV PLFD
Validated NT Sequence
gggtaccctg gaagctcaga cccagggtcc tggttcgatg caatatcctc ctcgcttggg ctttggaacg tggcaggcgc ctccagaggc cgtgcagacc gctgttgaga cagcgctcat gactgggtac aggcacatcg actgcgccta tgtctaccag aatgaggagg ccattggtcg tgcttttgga aagatcttca aggatgcgtc ttcaggaatc aagcgcgaag atgtctggat cacctcaaag ctatggaact ataaccatcg cccagaactg gtccgtgagc agtgcaagaa aaccatgtct gacttacagg tggattacct agatctcttc cttgtccact ggccgcttgc ctttgttcgt aatgacgtcg gggatctctt cccgaaggat gccgaaggcc gtgcgatgct cgagaaggtc cccctggccg acacatggag ggcaatggaa cagctggtcg aggagggcct tgtgaagcac atcggggtct cgaattacac ggtccctctt ttggccgatc tactaaacta cgccaagatc aagcctcttg tgaatcagat cgagatacac ccgtggcacc ccaacgacgc taccgtaaag ttctgccttg acaacggaat aggtgtgaca gcctactctc ccatgggtgg atcatatgca gatcccaggg atccctctgg cacacagaag aacgtgatcc ttgagtgcaa gacgctgaaa gcaatagccg atgccaaagg gacaagcccg cactgtgtgg cgctcgcttg gcacgtgaag aagtggaaca cctcgatgta cagtgtcatt cctaagagcc agacgcccgc gcgcattgag gccaacttca agtgcaccga agtacagctg agcgatgatg acatggatgc aatcaacaat attcacttga acaagagaat ccgcttttgc gatcccgcta tcttttggaa ggtaccactg tttgactaat aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQYPPRLGF GTWQAPPEAV QTAVETALMT GYRHIDCAYV YQNEEAIGRA FGKIFKDASS GIKREDVWIT SKLWNYNHRP ELVREQCKKT MSDLQVDYLD LFLVHWPLAF VRNDVGDLFP KDAEGRAMLE KVPLADTWRA MEQLVEEGLV KHIGVSNYTV PLLADLLNYA KIKPLVNQIE IHPWHPNDAT VKFCLDNGIG VTAYSPMGGS YADPRDPSGT QKNVILECKT LKAIADAKGT SPHCVALAWH VKKWNTSMYS VIPKSQTPAR IEANFKCTEV QLSDDDMDAI NNIHLNKRIR FCDPAIFWKV PLFD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaatatcct cctcgcttgg gctttggaac gtggcaggcg cctccagagg ccgtgcagac cgctgttgag acagcgctca tgactgggta caggcacatc gactgcgcct atgtctacca gaatgaggag gccattggtc gtgcttttgg aaagatcttc aaggatgcgt cttcaggaat caagcgcgaa gatgtctgga tcacctcaaa gctatggaac tataaccatc gcccagaact ggtccgtgag cagtgcaaga aaaccatgtc tgacttacag gtggattacc tagatctctt ccttgtccac tggccgcttg cctttgttcg taatgacgtc ggggatctct tcccgaagga tgccgaaggc cgtgcgatgc tcgagaaggt ccccctggcc gacacatgga gggcaatgga acagctggtc gaggagggcc ttgtgaagca catcggggtc tcgaattaca cggtccctct tttggccgat ctactaaact acgccaagat caagcctctt gtgaatcaga tcgagataca cccgtggcac cccaacgacg ctaccgtaaa gttctgcctt gacaacggaa taggtgtgac agcctactct cccatgggtg gatcatatgc agatcccagg gatccctctg gcacacagaa gaacgtgatc cttgagtgca agacgctgaa agcaatagcc gatgccaaag ggacaagccc gcactgtgtg gcgctcgctt ggcacgtgaa gaagtggaac acctcgatgt acagtgtcat tcctaagagc cagacgcccg cgcgcattga ggccaacttc aagtgcaccg aagtacagct gagcgatgat gacatggatg caatcaacaa tattcacttg aacaagagaa tccgcttttg cgatcccgct atcttttgga aggtaccact gtttgacaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for GilaA.01452.a.A1.PS00377
| PURIFICATION DATe: | 10/16/2009 |
| CONCENTRATION: | 23.25mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGP G SMQYPPRL GF GTWQAPP EAV QTAVET ALMT GYRHI DCAYV YQNE EAIGRA FGK IFKDASS GI KREDVWIT S KLWNYNHRP ELVREQCKKT MSDLQVDYL D LFLVHWPL AF VRNDVGD LFP KDAEGR AMLE KVPLA DTWRA MEQL VEEGLV KHI GVSNYTV PL LADLLNYA K IKPLVNQIE IHPWHPNDAT VKFCLDNGI G VTAYSPMG GS YADPRDP SGT QKNVIL ECKT LKAIA DAKGT SPHC VALAWH VKK WNTSMYS VI PKSQTPAR I EANFKCTEV QLSDDDMDAI NNIHLNKRI R FCDPAIFW KV PLFD
Validated NT Sequence
gggtaccctg gaagctcaga cccagggtcc tggttcgatg caatatcctc ctcgcttggg ctttggaacg tggcaggcgc ctccagaggc cgtgcagacc gctgttgaga cagcgctcat gactgggtac aggcacatcg actgcgccta tgtctaccag aatgaggagg ccattggtcg tgcttttgga aagatcttca aggatgcgtc ttcaggaatc aagcgcgaag atgtctggat cacctcaaag ctatggaact ataaccatcg cccagaactg gtccgtgagc agtgcaagaa aaccatgtct gacttacagg tggattacct agatctcttc cttgtccact ggccgcttgc ctttgttcgt aatgacgtcg gggatctctt cccgaaggat gccgaaggcc gtgcgatgct cgagaaggtc cccctggccg acacatggag ggcaatggaa cagctggtcg aggagggcct tgtgaagcac atcggggtct cgaattacac ggtccctctt ttggccgatc tactaaacta cgccaagatc aagcctcttg tgaatcagat cgagatacac ccgtggcacc ccaacgacgc taccgtaaag ttctgccttg acaacggaat aggtgtgaca gcctactctc ccatgggtgg atcatatgca gatcccaggg atccctctgg cacacagaag aacgtgatcc ttgagtgcaa gacgctgaaa gcaatagccg atgccaaagg gacaagcccg cactgtgtgg cgctcgcttg gcacgtgaag aagtggaaca cctcgatgta cagtgtcatt cctaagagcc agacgcccgc gcgcattgag gccaacttca agtgcaccga agtacagctg agcgatgatg acatggatgc aatcaacaat attcacttga acaagagaat ccgcttttgc gatcccgcta tcttttggaa ggtaccactg tttgactaat aa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQYPPRLGF GTWQAPPEAV QTAVETALMT GYRHIDCAYV YQNEEAIGRA FGKIFKDASS GIKREDVWIT SKLWNYNHRP ELVREQCKKT MSDLQVDYLD LFLVHWPLAF VRNDVGDLFP KDAEGRAMLE KVPLADTWRA MEQLVEEGLV KHIGVSNYTV PLLADLLNYA KIKPLVNQIE IHPWHPNDAT VKFCLDNGIG VTAYSPMGGS YADPRDPSGT QKNVILECKT LKAIADAKGT SPHCVALAWH VKKWNTSMYS VIPKSQTPAR IEANFKCTEV QLSDDDMDAI NNIHLNKRIR FCDPAIFWKV PLFD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAATATCCT CCTCGCTTGG GCTTTGGAAC GTGGCAGGCG CCTCCAGAGG CCGTGCAGAC CGCTGTTGAG ACAGCGCTCA TGACTGGGTA CAGGCACATC GACTGCGCCT ATGTCTACCA GAATGAGGAG GCCATTGGTC GTGCTTTTGG AAAGATCTTC AAGGATGCGT CTTCAGGAAT CAAGCGCGAA GATGTCTGGA TCACCTCAAA GCTATGGAAC TATAACCATC GCCCAGAACT GGTCCGTGAG CAGTGCAAGA AAACCATGTC TGACTTACAG GTGGATTACC TAGATCTCTT CCTTGTCCAC TGGCCGCTTG CCTTTGTTCG TAATGACGTC GGGGATCTCT TCCCGAAGGA TGCCGAAGGC CGTGCGATGC TCGAGAAGGT CCCCCTGGCC GACACATGGA GGGCAATGGA ACAGCTGGTC GAGGAGGGCC TTGTGAAGCA CATCGGGGTC TCGAATTACA CGGTCCCTCT TTTGGCCGAT CTACTAAACT ACGCCAAGAT CAAGCCTCTT GTGAATCAGA TCGAGATACA CCCGTGGCAC CCCAACGACG CTACCGTAAA GTTCTGCCTT GACAACGGAA TAGGTGTGAC AGCCTACTCT CCCATGGGTG GATCATATGC AGATCCCAGG GATCCCTCTG GCACACAGAA GAACGTGATC CTTGAGTGCA AGACGCTGAA AGCAATAGCC GATGCCAAAG GGACAAGCCC GCACTGTGTG GCGCTCGCTT GGCACGTGAA GAAGTGGAAC ACCTCGATGT ACAGTGTCAT TCCTAAGAGC CAGACGCCCG CGCGCATTGA GGCCAACTTC AAGTGCACCG AAGTACAGCT GAGCGATGAT GACATGGATG CAATCAACAA TATTCACTTG AACAAGAGAA TCCGCTTTTG CGATCCCGCT ATCTTTTGGA AGGTACCACT GTTTGACAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA