GilaA.01434.a

CDC8

CENTER ID: GilaA.01434.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.01434.a.A1.GE25745 Full length( GilaA.01434.a ) 1 254
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.01434.a.A1.PS00121 Full length( GilaA.01434.a ) 1 254

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_15380
RefSeq: XP_001704822.1
UniProt: A8BU63

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTHRSTNCQ PSNLQGSEYP EGRRKGDNVT RGLFVVIEGL DRAGKDTMIG QVVALLRQAG ESVVVQAFPD RTTKSGAVLD RFLQRELDLS DWDCHRLYAE NRKELEEVLC NTLLAGTTVI CSRYAYSGVA YTAAKGHDMR RCMEADKGLL RPDLVIFLDV DASAVAVRTG FGKERYETQD FQVKVYTAFQ TLWELLRNSQ NGSQDEEDRT TLLTIRPEDI SEAPKQILQA ILERKKRVRH TLCRDLFHLE LNLC
NT Sequence
ATGAGCACGC ACAGAAGCAC CAATTGTCAG CCCAGCAATT TGCAAGGTTC TGAATATCCA GAGGGAAGAA GGAAAGGAGA CAATGTCACC CGTGGTCTTT TTGTCGTCAT AGAAGGTCTT GATAGAGCTG GAAAGGACAC AATGATTGGG CAAGTAGTAG CTCTACTAAG GCAGGCCGGG GAGTCTGTCG TGGTACAAGC GTTCCCAGAC CGCACAACCA AATCCGGTGC CGTCTTGGAC CGCTTTTTGC AACGAGAGCT CGATCTCAGT GACTGGGACT GTCACAGGCT ATACGCTGAA AACAGAAAAG AACTCGAAGA AGTGCTATGC AATACATTGC TGGCTGGAAC AACGGTAATC TGCAGTCGTT ATGCCTATAG CGGTGTAGCA TACACGGCTG CTAAGGGGCA TGATATGCGT AGATGTATGG AGGCAGACAA AGGCCTTCTA CGACCAGATT TAGTAATCTT TCTTGACGTC GATGCAAGTG CCGTTGCAGT GCGAACTGGC TTTGGAAAGG AACGTTACGA AACACAGGAC TTCCAAGTAA AGGTCTATAC TGCTTTCCAA ACACTTTGGG AGCTTCTTCG CAATTCTCAA AATGGGTCCC AGGATGAGGA AGATCGTACT ACTTTGCTCA CCATCCGTCC CGAAGACATC TCCGAGGCTC CCAAGCAGAT CCTACAGGCC ATTCTTGAGC GTAAGAAGAG GGTCCGGCAC ACCCTATGCA GAGACTTATT TCATTTAGAA TTGAATTTGT GT
Details for GilaA.01434.a.A1.GE25745
HARVESTED ON: 10/31/2008
SEQUENCED ON: 8/18/2010
EXPECTED MW: 31kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTHRSTNC QPSNLQGSEY PEGRRKGDNV TRGLFVVIEG LDRAGKDTMI GQVVALLRQA GESVVVQAFP DRTTKSGAVL DRFLQRELDL SDWDCHRLYA ENRKELEEVL CNTLLAGTTV ICSRYAYSGV AYTAAKGHDM RRCMEADKGL LRPDLVIFLD VDASAVAVRT GFGKERYETQ DFQVKVYTAF QTLWELLRNS QNGSQDEEDR TTLLTIRPED ISEAPKQILQ AILERKKRVR HTLCRDLFHL ELNLC
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagca cgcacagaag caccaattgt cagcccagca atttgcaagg ttctgaatat ccagagggaa gaaggaaagg agacaatgtc acccgtggtc tttttgtcgt catagaaggt cttgatagag ctggaaagga cacaatgatt gggcaagtag tagctctact aaggcaggcc ggggagtctg tcgtggtaca agcgttccca gaccgcacaa ccaaatccgg tgccgtcttg gaccgctttt tgcaacgaga gctcgatctc agtgactggg actgtcacag gctatacgct gaaaacagaa aagaactcga agaagtgcta tgcaatacat tgctggctgg aacaacggta atctgcagtc gttatgccta tagcggtgta gcatacacgg ctgctaaggg gcatgatatg cgtagatgta tggaggcaga caaaggcctt ctacgaccag atttagtaat ctttcttgac gtcgatgcaa gtgccgttgc agtgcgaact ggctttggaa aggaacgtta cgaaacacag gacttccaag taaaggtcta tactgctttc caaacacttt gggagcttct tcgcaattct caaaatgggt cccaggatga ggaagatcgt actactttgc tcaccatccg tcccgaagac atctccgagg ctcccaagca gatcctacag gccattcttg agcgtaagaa gagggtccgg cacaccctat gcagagactt atttcattta gaattgaatt tgtgttaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTHRSTNC QPSNLQGSEY PEGRRKGDNV TRGLFVVIEG LDRAGKDTMI GQVVALLRQA GESVVVQAFP DRTTKSGAVL DRFLQRELDL SDWDCHRLYA ENRKELEEVL CNTLLAGTTV ICSRYAYSGV AYTAAKGHDM RRCMEADKGL LRPDLVIFLD VDASAVAVRT GFGKERYETQ DFQVKVYTAF QTLWELLRNS QNGSQDEEDR TTLLTIRPED ISEAPKQILQ AILERKKRVR HTLCRDLFHL ELNLC
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcacgcac agaagcacca attgtcagcc cagcaatttg caaggttctg aatatccaga gggaagaagg aaaggagaca atgtcacccg tggtcttttt gtcgtcatag aaggtcttga tagagctgga aaggacacaa tgattgggca agtagtagct ctactaaggc aggccgggga gtctgtcgtg gtacaagcgt tcccagaccg cacaaccaaa tccggtgccg tcttggaccg ctttttgcaa cgagagctcg atctcagtga ctgggactgt cacaggctat acgctgaaaa cagaaaagaa ctcgaagaag tgctatgcaa tacattgctg gctggaacaa cggtaatctg cagtcgttat gcctatagcg gtgtagcata cacggctgct aaggggcatg atatgcgtag atgtatggag gcagacaaag gccttctacg accagattta gtaatctttc ttgacgtcga tgcaagtgcc gttgcagtgc gaactggctt tggaaaggaa cgttacgaaa cacaggactt ccaagtaaag gtctatactg ctttccaaac actttgggag cttcttcgca attctcaaaa tgggtcccag gatgaggaag atcgtactac tttgctcacc atccgtcccg aagacatctc cgaggctccc aagcagatcc tacaggccat tcttgagcgt aagaagaggg tccggcacac cctatgcaga gacttatttc atttagaatt gaatttgtgt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for GilaA.01434.a.A1.PS00121
PURIFICATION DATe: 1/23/2009
CONCENTRATION: 56.8mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTHRSTNC QPSNLQGSEY PEGRRKGDNV TRGLFVVIEG LDRAGKDTMI GQVVALLRQA GESVVVQAFP DRTTKSGAVL DRFLQRELDL SDWDCHRLYA ENRKELEEVL CNTLLAGTTV ICSRYAYSGV AYTAAKGHDM RRCMEADKGL LRPDLVIFLD VDASAVAVRT GFGKERYETQ DFQVKVYTAF QTLWELLRNS QNGSQDEEDR TTLLTIRPED ISEAPKQILQ AILERKKRVR HTLCRDLFHL ELNLC
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagca cgcacagaag caccaattgt cagcccagca atttgcaagg ttctgaatat ccagagggaa gaaggaaagg agacaatgtc acccgtggtc tttttgtcgt catagaaggt cttgatagag ctggaaagga cacaatgatt gggcaagtag tagctctact aaggcaggcc ggggagtctg tcgtggtaca agcgttccca gaccgcacaa ccaaatccgg tgccgtcttg gaccgctttt tgcaacgaga gctcgatctc agtgactggg actgtcacag gctatacgct gaaaacagaa aagaactcga agaagtgcta tgcaatacat tgctggctgg aacaacggta atctgcagtc gttatgccta tagcggtgta gcatacacgg ctgctaaggg gcatgatatg cgtagatgta tggaggcaga caaaggcctt ctacgaccag atttagtaat ctttcttgac gtcgatgcaa gtgccgttgc agtgcgaact ggctttggaa aggaacgtta cgaaacacag gacttccaag taaaggtcta tactgctttc caaacacttt gggagcttct tcgcaattct caaaatgggt cccaggatga ggaagatcgt actactttgc tcaccatccg tcccgaagac atctccgagg ctcccaagca gatcctacag gccattcttg agcgtaagaa gagggtccgg cacaccctat gcagagactt atttcattta gaattgaatt tgtgttaata a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTHRSTNC QPSNLQGSEY PEGRRKGDNV TRGLFVVIEG LDRAGKDTMI GQVVALLRQA GESVVVQAFP DRTTKSGAVL DRFLQRELDL SDWDCHRLYA ENRKELEEVL CNTLLAGTTV ICSRYAYSGV AYTAAKGHDM RRCMEADKGL LRPDLVIFLD VDASAVAVRT GFGKERYETQ DFQVKVYTAF QTLWELLRNS QNGSQDEEDR TTLLTIRPED ISEAPKQILQ AILERKKRVR HTLCRDLFHL ELNLC
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCACGCAC AGAAGCACCA ATTGTCAGCC CAGCAATTTG CAAGGTTCTG AATATCCAGA GGGAAGAAGG AAAGGAGACA ATGTCACCCG TGGTCTTTTT GTCGTCATAG AAGGTCTTGA TAGAGCTGGA AAGGACACAA TGATTGGGCA AGTAGTAGCT CTACTAAGGC AGGCCGGGGA GTCTGTCGTG GTACAAGCGT TCCCAGACCG CACAACCAAA TCCGGTGCCG TCTTGGACCG CTTTTTGCAA CGAGAGCTCG ATCTCAGTGA CTGGGACTGT CACAGGCTAT ACGCTGAAAA CAGAAAAGAA CTCGAAGAAG TGCTATGCAA TACATTGCTG GCTGGAACAA CGGTAATCTG CAGTCGTTAT GCCTATAGCG GTGTAGCATA CACGGCTGCT AAGGGGCATG ATATGCGTAG ATGTATGGAG GCAGACAAAG GCCTTCTACG ACCAGATTTA GTAATCTTTC TTGACGTCGA TGCAAGTGCC GTTGCAGTGC GAACTGGCTT TGGAAAGGAA CGTTACGAAA CACAGGACTT CCAAGTAAAG GTCTATACTG CTTTCCAAAC ACTTTGGGAG CTTCTTCGCA ATTCTCAAAA TGGGTCCCAG GATGAGGAAG ATCGTACTAC TTTGCTCACC ATCCGTCCCG AAGACATCTC CGAGGCTCCC AAGCAGATCC TACAGGCCAT TCTTGAGCGT AAGAAGAGGG TCCGGCACAC CCTATGCAGA GACTTATTTC ATTTAGAATT GAATTTGTGT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA