SSGCID: Target Page

GilaA.01401.a

Uracil phosphoribosyltransferase

CENTER ID:	GilaA.01401.a
ORGANISM:	Giardia lamblia ATCC 50803
ASSOCIATED DISEASE:	Giardiasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
GilaA.01401.a.A1.GE25742	Full length( GilaA.01401.a )		1	211

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	GiardiaDB:GL50803_6563
RefSeq:	XP_001706678.1
UniProt:	Q86QI1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGLTIFDHPL IQHKVGLLRS VTTGPKEFRE LLREIGLLMM YEATRSLELE DYEIATPLCT TVGKRLRGKK AAVVPILRAG LGMVDGLLEI LPAAKVGHMG LYRDHDTLQP VTYFCKMPVD MAERSIYIVD PMLATAGSVI ATIDTLVKKY NCPLSSIKVM VIIAAPEGID NLRKAYPDIE VFCAQKDSHL NEKGYIVPGL GDAGDRIFGT L

NT Sequence

ATGGGCCTCA CTATCTTCGA CCACCCGCTC ATCCAACACA AGGTCGGGCT CCTCCGCAGC GTCACAACCG GCCCGAAGGA GTTCCGGGAG CTCCTCAGGG AGATCGGCCT CCTCATGATG TACGAGGCCA CCCGCAGCCT CGAGCTGGAG GACTACGAGA TCGCCACGCC CCTCTGCACA ACCGTCGGCA AGCGCCTCCG CGGGAAGAAG GCAGCGGTCG TTCCCATTCT GAGAGCAGGC CTCGGCATGG TCGATGGGCT TCTGGAGATC CTGCCCGCCG CGAAGGTTGG ACACATGGGG CTTTACAGGG ACCACGATAC CCTGCAGCCG GTGACATACT TCTGCAAGAT GCCCGTTGAC ATGGCCGAGA GGTCCATCTA CATAGTCGAT CCTATGCTTG CCACAGCAGG ATCGGTCATC GCAACTATCG ATACCCTTGT TAAGAAGTAC AACTGTCCTC TTTCGTCCAT TAAGGTCATG GTCATCATTG CAGCCCCAGA GGGCATCGAC AATCTTCGCA AGGCGTATCC AGACATAGAG GTGTTTTGTG CACAGAAGGA TAGCCACCTC AACGAGAAGG GCTACATTGT GCCGGGGCTC GGGGATGCCG GTGACCGTAT CTTTGGTACC TTA

Details for GilaA.01401.a.A1.GE25742

HARVESTED ON:	10/31/2008
SEQUENCED ON:	7/20/2010
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMGLTIFDHP LIQHKVGLLR SVTTGPKEFR ELLREIGLLM MYEATRSLEL EDYEIATPLC TTVGKRLRGK KAAVVPILRA GLGMVDGLLE ILPAAKVGHM GLYRDHDTLQ PVTYFCKMPV DMAERSIYIV DPMLATAGSV IATIDTLVKK YNCPLSSIKV MVIIAAPEGI DNLRKAYPDI EVFCAQKDSH LNEKGYIVPG LGDAGDRIFG TL

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggcc tcactatctt cgaccacccg ctcatccaac acaaggtcgg gctcctccgc agcgtcacaa ccggcccgaa ggagttccgg gagctcctca gggagatcgg cctcctcatg atgtacgagg ccacccgcag cctcgagctg gaggactacg agatcgccac gcccctctgc acaaccgtcg gcaagcgcct ccgcgggaag aaggcagcgg tcgttcccat tctgagagca ggcctcggca tggtcgatgg gcttctggag atcctgcccg ccgcgaaggt tggacacatg gggctttaca gggaccacga taccctgcag ccggtgacat acttctgcaa gatgcccgtt gacatggccg agaggtccat ctacatagtc gatcctatgc ttgccacagc aggatcggtc atcgcaacta tcgataccct tgttaagaag tacaactgtc ctctttcgtc cattaaggtc atggtcatca ttgcagcccc agagggcatc gacaatcttc gcaaggcgta tccagacata gaggtgtttt gtgcacagaa ggatagccac ctcaacgaga agggctacat tgtgccgggg ctcggggatg ccggtgaccg tatctttggt accttataat aa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcctcact atcttcgacc acccgctcat ccaacacaag gtcgggctcc tccgcagcgt cacaaccggc ccgaaggagt tccgggagct cctcagggag atcggcctcc tcatgatgta cgaggccacc cgcagcctcg agctggagga ctacgagatc gccacgcccc tctgcacaac cgtcggcaag cgcctccgcg ggaagaaggc agcggtcgtt cccattctga gagcaggcct cggcatggtc gatgggcttc tggagatcct gcccgccgcg aaggttggac acatggggct ttacagggac cacgataccc tgcagccggt gacatacttc tgcaagatgc ccgttgacat ggccgagagg tccatctaca tagtcgatcc tatgcttgcc acagcaggat cggtcatcgc aactatcgat acccttgtta agaagtacaa ctgtcctctt tcgtccatta aggtcatggt catcattgca gccccagagg gcatcgacaa tcttcgcaag gcgtatccag acatagaggt gttttgtgca cagaaggata gccacctcaa cgagaagggc tacattgtgc cggggctcgg ggatgccggt gaccgtatct ttggtacctt aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa