GilaA.01269.a

Kinase, CMGC CDK

CENTER ID: GilaA.01269.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.01269.a.A1.GE25735 Full length( GilaA.01269.a ) 1 340
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.01269.a.A1.PW27596 Full length( GilaA.01269.a ) 1 340

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_15397
RefSeq: XP_001708623.1
UniProt: A8B8J3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATHPLPKDY EFISQIGEGT YGTVSKCSLH GQLLAVKRIK MDSSSKGVPA TTLREVAILK ALNAKQRELV EQGKLESLSQ ANVVNLLDVV YDLSNIFLVF EYCHVDLDQY LKHNVLSPYQ IKKFMYQLLQ GLSLVHKNEI LHRDIKPNNI LLAFRGDAGE QLAANASVTT PNPDADNCVL KITDFGLARS YGINVTSISA NVVSLWYRAP ELVLASKTYS TAVDMWSMGC IMYFLANKRA LFQVRKDADL LQLAFSYFYV DSSSISDLIS LPNWNMYVGE NQVIDLAALN APGRKPREVD INIDDVGKDL FYKLLMLNPS KRISCDEALQ HPWFDDLKKG
NT Sequence
ATGGCTACTC ATCCACTGCC TAAGGACTAC GAATTTATCT CGCAGATTGG CGAGGGGACC TACGGGACTG TTAGTAAGTG TTCTCTCCAC GGGCAGTTGC TTGCGGTGAA ACGTATCAAG ATGGATTCGT CCTCCAAAGG GGTTCCGGCT ACGACGCTCC GCGAGGTGGC GATTCTGAAG GCCCTCAATG CGAAACAGCG TGAGTTGGTG GAGCAAGGAA AGCTTGAAAG TCTGTCACAG GCGAATGTGG TTAATCTTTT AGACGTTGTT TATGACTTGA GCAACATATT TCTAGTTTTT GAGTACTGTC ACGTTGACCT AGATCAGTAT CTCAAACACA ATGTCCTGTC TCCTTACCAG ATAAAAAAGT TCATGTACCA GCTCCTCCAG GGCCTCAGTT TGGTTCATAA GAACGAGATC TTGCATCGTG ACATCAAGCC CAACAACATC TTGTTGGCCT TTCGCGGAGA TGCAGGGGAA CAATTGGCCG CCAATGCGTC TGTGACTACG CCGAATCCGG ACGCAGACAA TTGTGTTTTG AAGATTACGG ACTTCGGCCT TGCACGGTCA TACGGCATAA ACGTGACGTC CATCTCGGCC AATGTCGTCT CCCTGTGGTA CCGGGCTCCC GAGCTTGTTC TAGCCTCGAA GACGTACTCC ACGGCTGTCG ACATGTGGTC CATGGGGTGC ATAATGTACT TCCTTGCGAA TAAGAGGGCC CTGTTTCAGG TTCGAAAGGA TGCAGATCTG CTGCAGCTGG CGTTTAGCTA CTTCTATGTC GACTCCTCTT CTATTTCTGA CCTCATTTCT CTGCCCAACT GGAATATGTA CGTTGGAGAG AACCAGGTCA TCGACTTAGC GGCGCTCAAT GCCCCGGGGC GCAAGCCCCG TGAGGTAGAC ATTAACATAG ATGACGTCGG TAAAGACCTC TTCTACAAGC TCCTGATGCT GAACCCCAGC AAGCGAATTT CGTGCGACGA AGCTCTCCAG CATCCGTGGT TCGACGACCT AAAGAAGGGC
Details for GilaA.01269.a.A1.GE25735
HARVESTED ON: 10/31/2008
SEQUENCED ON: 7/20/2010
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMATHPLPKD YEFISQIGEG TYGTVSKCSL HGQLLAVKRI KMDSSSKGVP ATTLREVAIL KALNAKQREL VEQGKLESLS QANVVNLLDV VYDLSNIFLV FEYCHVDLDQ YLKHNVLSPY QIKKFMYQLL QGLSLVHKNE ILHRDIKPNN ILLAFRGDAG EQLAANASVT TPNPDADNCV LKITDFGLAR SYGINVTSIS ANVVSLWYRA PELVLASKTY STAVDMWSMG CIMYFLANKR ALFQVRKDAD LLQLAFSYFY VDSSSISDLI SLPNWNMYVG ENQVIDLAAL NAPGRKPREV DINIDDVGKD LFYKLLMLNP SKRISCDEAL QHPWFDDLK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcta ctcatccact gcctaaggac tacgaattta tctcgcagat tggcgagggg acctacggga ctgttagtaa gtgttctctc cacgggcagt tgcttgcggt gaaacgtatc aagatggatt cgtcctccaa aggggttccg gctacgacgc tccgcgaggt ggcgattctg aaggccctca atgcgaaaca gcgtgagttg gtggagcaag gaaagcttga aagtctgtca caggcgaatg tggttaatct tttagacgtt gtttatgact tgagcaacat atttctagtt tttgagtact gtcacgttga cctagatcag tatctcaaac acaatgtcct gtctccttac cagataaaaa agttcatgta ccagctcctc cagggcctca gtttggttca taagaacgag atcttgcatc gtgacatcaa gcccaacaac atcttgttgg cctttcgcgg agatgcaggg gaacaattgg ccgccaatgc gtctgtgact acgccgaatc cggacgcaga caattgtgtt ttgaagatta cggacttcgg ccttgcacgg tcatacggca taaacgtgac gtccatctcg gccaatgtcg tctccctgtg gtaccgggct cccgagcttg ttctagcctc gaagacgtac tccacggctg tcgacatgtg gtccatgggg tgcataatgt acttccttgc gaataagagg gccctgtttc aggttcgaaa ggatgcagat ctgctgcagc tggcgtttag ctacttctat gtcgactcct cttctatttc tgacctcatt tctctgccca actggaatat gtacgttgga gagaaccagg tcatcgactt agcggcgctc aatgccccgg ggcgcaagcc ccgtgaggta gacattaaca tagatgacgt cggtaaagac ctcttctaca agctcctgat gctgaacccc agcaagcgaa tttcgtgcga cgaagctctc cagcatccgt ggttcgacga cctaaaga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMATHPLPKD YEFISQIGEG TYGTVSKCSL HGQLLAVKRI KMDSSSKGVP ATTLREVAIL KALNAKQREL VEQGKLESLS QANVVNLLDV VYDLSNIFLV FEYCHVDLDQ YLKHNVLSPY QIKKFMYQLL QGLSLVHKNE ILHRDIKPNN ILLAFRGDAG EQLAANASVT TPNPDADNCV LKITDFGLAR SYGINVTSIS ANVVSLWYRA PELVLASKTY STAVDMWSMG CIMYFLANKR ALFQVRKDAD LLQLAFSYFY VDSSSISDLI SLPNWNMYVG ENQVIDLAAL NAPGRKPREV DINIDDVGKD LFYKLLMLNP SKRISCDEAL QHPWFDDLKK G
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctactcat ccactgccta aggactacga atttatctcg cagattggcg aggggaccta cgggactgtt agtaagtgtt ctctccacgg gcagttgctt gcggtgaaac gtatcaagat ggattcgtcc tccaaagggg ttccggctac gacgctccgc gaggtggcga ttctgaaggc cctcaatgcg aaacagcgtg agttggtgga gcaaggaaag cttgaaagtc tgtcacaggc gaatgtggtt aatcttttag acgttgttta tgacttgagc aacatatttc tagtttttga gtactgtcac gttgacctag atcagtatct caaacacaat gtcctgtctc cttaccagat aaaaaagttc atgtaccagc tcctccaggg cctcagtttg gttcataaga acgagatctt gcatcgtgac atcaagccca acaacatctt gttggccttt cgcggagatg caggggaaca attggccgcc aatgcgtctg tgactacgcc gaatccggac gcagacaatt gtgttttgaa gattacggac ttcggccttg cacggtcata cggcataaac gtgacgtcca tctcggccaa tgtcgtctcc ctgtggtacc gggctcccga gcttgttcta gcctcgaaga cgtactccac ggctgtcgac atgtggtcca tggggtgcat aatgtacttc cttgcgaata agagggccct gtttcaggtt cgaaaggatg cagatctgct gcagctggcg tttagctact tctatgtcga ctcctcttct atttctgacc tcatttctct gcccaactgg aatatgtacg ttggagagaa ccaggtcatc gacttagcgg cgctcaatgc cccggggcgc aagccccgtg aggtagacat taacatagat gacgtcggta aagacctctt ctacaagctc ctgatgctga accccagcaa gcgaatttcg tgcgacgaag ctctccagca tccgtggttc gacgacctaa agaagggcaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for GilaA.01269.a.A1.PW27596
PURIFICATION DATe: 10/19/2009
CONCENTRATION: 23.64mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 22
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMATHPLPKD YEFISQIGEG TYGTVSKCSL HGQLLAVKRI KMDSSSKGVP ATTLREVAIL KALNAKQREL VEQGKLESLS QANVVNLLDV VYDLSNIFLV FEYCHVDLDQ YLKHNVLSPY QIKKFMYQLL QGLSLVHKNE ILHRDIKPNN ILLAFRGDAG EQLAANASVT TPNPDADNCV LKITDFGLAR SYGINVTSIS ANVVSLWYRA PELVLASKTY STAVDMWSMG CIMYFLANKR ALFQVRKDAD LLQLAFSYFY VDSSSISDLI SLPNWNMYVG ENQVIDLAAL NAPGRKPREV DINIDDVGKD LFYKLLMLNP SKRISCDEAL QHPWFDDLK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcta ctcatccact gcctaaggac tacgaattta tctcgcagat tggcgagggg acctacggga ctgttagtaa gtgttctctc cacgggcagt tgcttgcggt gaaacgtatc aagatggatt cgtcctccaa aggggttccg gctacgacgc tccgcgaggt ggcgattctg aaggccctca atgcgaaaca gcgtgagttg gtggagcaag gaaagcttga aagtctgtca caggcgaatg tggttaatct tttagacgtt gtttatgact tgagcaacat atttctagtt tttgagtact gtcacgttga cctagatcag tatctcaaac acaatgtcct gtctccttac cagataaaaa agttcatgta ccagctcctc cagggcctca gtttggttca taagaacgag atcttgcatc gtgacatcaa gcccaacaac atcttgttgg cctttcgcgg agatgcaggg gaacaattgg ccgccaatgc gtctgtgact acgccgaatc cggacgcaga caattgtgtt ttgaagatta cggacttcgg ccttgcacgg tcatacggca taaacgtgac gtccatctcg gccaatgtcg tctccctgtg gtaccgggct cccgagcttg ttctagcctc gaagacgtac tccacggctg tcgacatgtg gtccatgggg tgcataatgt acttccttgc gaataagagg gccctgtttc aggttcgaaa ggatgcagat ctgctgcagc tggcgtttag ctacttctat gtcgactcct cttctatttc tgacctcatt tctctgccca actggaatat gtacgttgga gagaaccagg tcatcgactt agcggcgctc aatgccccgg ggcgcaagcc ccgtgaggta gacattaaca tagatgacgt cggtaaagac ctcttctaca agctcctgat gctgaacccc agcaagcgaa tttcgtgcga cgaagctctc cagcatccgt ggttcgacga cctaaaga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMATHPLPKD YEFISQIGEG TYGTVSKCSL HGQLLAVKRI KMDSSSKGVP ATTLREVAIL KALNAKQREL VEQGKLESLS QANVVNLLDV VYDLSNIFLV FEYCHVDLDQ YLKHNVLSPY QIKKFMYQLL QGLSLVHKNE ILHRDIKPNN ILLAFRGDAG EQLAANASVT TPNPDADNCV LKITDFGLAR SYGINVTSIS ANVVSLWYRA PELVLASKTY STAVDMWSMG CIMYFLANKR ALFQVRKDAD LLQLAFSYFY VDSSSISDLI SLPNWNMYVG ENQVIDLAAL NAPGRKPREV DINIDDVGKD LFYKLLMLNP SKRISCDEAL QHPWFDDLKK G
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTACTCAT CCACTGCCTA AGGACTACGA ATTTATCTCG CAGATTGGCG AGGGGACCTA CGGGACTGTT AGTAAGTGTT CTCTCCACGG GCAGTTGCTT GCGGTGAAAC GTATCAAGAT GGATTCGTCC TCCAAAGGGG TTCCGGCTAC GACGCTCCGC GAGGTGGCGA TTCTGAAGGC CCTCAATGCG AAACAGCGTG AGTTGGTGGA GCAAGGAAAG CTTGAAAGTC TGTCACAGGC GAATGTGGTT AATCTTTTAG ACGTTGTTTA TGACTTGAGC AACATATTTC TAGTTTTTGA GTACTGTCAC GTTGACCTAG ATCAGTATCT CAAACACAAT GTCCTGTCTC CTTACCAGAT AAAAAAGTTC ATGTACCAGC TCCTCCAGGG CCTCAGTTTG GTTCATAAGA ACGAGATCTT GCATCGTGAC ATCAAGCCCA ACAACATCTT GTTGGCCTTT CGCGGAGATG CAGGGGAACA ATTGGCCGCC AATGCGTCTG TGACTACGCC GAATCCGGAC GCAGACAATT GTGTTTTGAA GATTACGGAC TTCGGCCTTG CACGGTCATA CGGCATAAAC GTGACGTCCA TCTCGGCCAA TGTCGTCTCC CTGTGGTACC GGGCTCCCGA GCTTGTTCTA GCCTCGAAGA CGTACTCCAC GGCTGTCGAC ATGTGGTCCA TGGGGTGCAT AATGTACTTC CTTGCGAATA AGAGGGCCCT GTTTCAGGTT CGAAAGGATG CAGATCTGCT GCAGCTGGCG TTTAGCTACT TCTATGTCGA CTCCTCTTCT ATTTCTGACC TCATTTCTCT GCCCAACTGG AATATGTACG TTGGAGAGAA CCAGGTCATC GACTTAGCGG CGCTCAATGC CCCGGGGCGC AAGCCCCGTG AGGTAGACAT TAACATAGAT GACGTCGGTA AAGACCTCTT CTACAAGCTC CTGATGCTGA ACCCCAGCAA GCGAATTTCG TGCGACGAAG CTCTCCAGCA TCCGTGGTTC GACGACCTAA AGAAGGGCAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA