GilaA.01262.a

Rab2b

CENTER ID: GilaA.01262.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.01262.a.A1.GE25732 Full length( GilaA.01262.a ) 1 227
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.01262.a.A1.PS00120 Full length( GilaA.01262.a ) 1 227

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_16636
RefSeq: XP_001709135.1
UniProt: A8B742

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSGGHHYIF KYIIVGDSAV GKSCLLLRFV EQKFIPLHDV TVGVEFCNKT LQVPSKDGSE KTVKVQIWDT AGQEQFRSIT RSYYRGAAGA LLVYDVTNRS SFEALEGWLK DMLAGVGMEA SPNGVSGMVI MLVGNKTDLE HKRKVSKEEG EQFARDHGLL FIETSAKDDL NVSESFNETA RTILEKVETG EIDCVSKDSG VKAGALNMSA AATTASSTDS TFGNCSC
NT Sequence
ATGTCCAGCG GCGGACACCA TTATATCTTC AAGTACATCA TCGTAGGCGA CTCTGCGGTC GGAAAGTCCT GCCTCTTGTT GAGGTTCGTT GAACAGAAGT TTATCCCTCT GCATGATGTA ACAGTGGGAG TGGAGTTTTG CAACAAGACC CTTCAGGTGC CCTCGAAAGA TGGCTCTGAA AAGACCGTCA AGGTGCAGAT TTGGGACACT GCAGGCCAGG AGCAGTTTCG TTCCATAACA AGAAGCTATT ACAGAGGAGC GGCCGGCGCT CTTCTCGTTT ATGACGTCAC GAACAGGAGT AGCTTTGAAG CCCTTGAGGG TTGGCTTAAG GACATGCTCG CCGGGGTGGG CATGGAAGCC AGTCCGAACG GCGTTAGCGG CATGGTTATT ATGTTAGTGG GAAACAAGAC AGATCTCGAG CACAAGAGGA AAGTCTCGAA GGAAGAGGGC GAGCAGTTTG CCCGTGACCA CGGCCTTCTC TTCATCGAAA CCTCAGCAAA AGACGACCTG AACGTCTCGG AGAGCTTTAA CGAGACGGCG CGGACCATTC TTGAGAAAGT GGAAACAGGG GAGATCGACT GCGTCTCTAA GGATAGTGGG GTGAAGGCAG GGGCTCTCAA TATGAGCGCT GCAGCGACCA CTGCCTCGTC CACTGACAGC ACATTTGGAA ATTGCTCCTG T
Details for GilaA.01262.a.A1.GE25732
HARVESTED ON: 10/31/2008
SEQUENCED ON: 7/20/2010
EXPECTED MW: 26kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSSGGHRYI FKYIIVGDSA VGKSCLLLRF VEQKFIPLHD VTVGVEFCNK TLQVPSKDGS EKTVKVQIWD TAGQEQFRSI TRSYYRGAAG ALLVYDVTNR SSFEALEGWL KDMLAGVGME ASPNGVSGMV IMLVGNKTDL EHKRKVSKEE GEQFARDHGL LFIETSAKDD LNVSESFNET ARTILEKVET GEIDCVSKDS GVKAGALNMS AAATTASSTD STFGNCSC
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcca gcggcggaca ccgttatatc ttcaagtaca tcatcgtagg cgactctgcg gtcggaaagt cctgcctctt gttgaggttc gttgaacaga agtttatccc tctgcatgat gtaacagtgg gagtggagtt ttgcaacaag acccttcagg tgccctcgaa agatggctct gaaaagaccg tcaaggtgca gatttgggac actgcaggcc aggagcagtt tcgttccata acaagaagct attacagagg agcggccggc gctcttctcg tttatgacgt cacgaacagg agtagctttg aagcccttga gggttggctt aaggacatgc tcgccggggt gggcatggaa gccagtccga acggcgttag cggcatggtt attatgttag tgggaaacaa gacagatctc gagcacaaga ggaaagtctc gaaggaagag ggcgagcagt ttgcccgtga ccacggcctt ctcttcatcg aaacctcagc aaaagacgac ctgaacgtct cggagagctt taacgagacg gcgcggacca ttcttgagaa agtggaaaca ggggagatcg actgcgtctc taaggatagt ggggtgaagg caggggctct caatatgagc gctgcagcga ccactgcctc gtccactgac agcacatttg gaaattgctc ctgttaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSGGHHYI FKYIIVGDSA VGKSCLLLRF VEQKFIPLHD VTVGVEFCNK TLQVPSKDGS EKTVKVQIWD TAGQEQFRSI TRSYYRGAAG ALLVYDVTNR SSFEALEGWL KDMLAGVGME ASPNGVSGMV IMLVGNKTDL EHKRKVSKEE GEQFARDHGL LFIETSAKDD LNVSESFNET ARTILEKVET GEIDCVSKDS GVKAGALNMS AAATTASSTD STFGNCSC
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccagcggc ggacaccatt atatcttcaa gtacatcatc gtaggcgact ctgcggtcgg aaagtcctgc ctcttgttga ggttcgttga acagaagttt atccctctgc atgatgtaac agtgggagtg gagttttgca acaagaccct tcaggtgccc tcgaaagatg gctctgaaaa gaccgtcaag gtgcagattt gggacactgc aggccaggag cagtttcgtt ccataacaag aagctattac agaggagcgg ccggcgctct tctcgtttat gacgtcacga acaggagtag ctttgaagcc cttgagggtt ggcttaagga catgctcgcc ggggtgggca tggaagccag tccgaacggc gttagcggca tggttattat gttagtggga aacaagacag atctcgagca caagaggaaa gtctcgaagg aagagggcga gcagtttgcc cgtgaccacg gccttctctt catcgaaacc tcagcaaaag acgacctgaa cgtctcggag agctttaacg agacggcgcg gaccattctt gagaaagtgg aaacagggga gatcgactgc gtctctaagg atagtggggt gaaggcaggg gctctcaata tgagcgctgc agcgaccact gcctcgtcca ctgacagcac atttggaaat tgctcctgta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for GilaA.01262.a.A1.PS00120
PURIFICATION DATe: 1/9/2009
CONCENTRATION: 23.16mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSSGGHRYI FKYIIVGDSA VGKSCLLLRF VEQKFIPLHD VTVGVEFCNK TLQVPSKDGS EKTVKVQIWD TAGQEQFRSI TRSYYRGAAG ALLVYDVTNR SSFEALEGWL KDMLAGVGME ASPNGVSGMV IMLVGNKTDL EHKRKVSKEE GEQFARDHGL LFIETSAKDD LNVSESFNET ARTILEKVET GEIDCVSKDS GVKAGALNMS AAATTASSTD STFGNCSC
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcca gcggcggaca ccgttatatc ttcaagtaca tcatcgtagg cgactctgcg gtcggaaagt cctgcctctt gttgaggttc gttgaacaga agtttatccc tctgcatgat gtaacagtgg gagtggagtt ttgcaacaag acccttcagg tgccctcgaa agatggctct gaaaagaccg tcaaggtgca gatttgggac actgcaggcc aggagcagtt tcgttccata acaagaagct attacagagg agcggccggc gctcttctcg tttatgacgt cacgaacagg agtagctttg aagcccttga gggttggctt aaggacatgc tcgccggggt gggcatggaa gccagtccga acggcgttag cggcatggtt attatgttag tgggaaacaa gacagatctc gagcacaaga ggaaagtctc gaaggaagag ggcgagcagt ttgcccgtga ccacggcctt ctcttcatcg aaacctcagc aaaagacgac ctgaacgtct cggagagctt taacgagacg gcgcggacca ttcttgagaa agtggaaaca ggggagatcg actgcgtctc taaggatagt ggggtgaagg caggggctct caatatgagc gctgcagcga ccactgcctc gtccactgac agcacatttg gaaattgctc ctgttaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSGGHHYI FKYIIVGDSA VGKSCLLLRF VEQKFIPLHD VTVGVEFCNK TLQVPSKDGS EKTVKVQIWD TAGQEQFRSI TRSYYRGAAG ALLVYDVTNR SSFEALEGWL KDMLAGVGME ASPNGVSGMV IMLVGNKTDL EHKRKVSKEE GEQFARDHGL LFIETSAKDD LNVSESFNET ARTILEKVET GEIDCVSKDS GVKAGALNMS AAATTASSTD STFGNCSC
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCAGCGGC GGACACCATT ATATCTTCAA GTACATCATC GTAGGCGACT CTGCGGTCGG AAAGTCCTGC CTCTTGTTGA GGTTCGTTGA ACAGAAGTTT ATCCCTCTGC ATGATGTAAC AGTGGGAGTG GAGTTTTGCA ACAAGACCCT TCAGGTGCCC TCGAAAGATG GCTCTGAAAA GACCGTCAAG GTGCAGATTT GGGACACTGC AGGCCAGGAG CAGTTTCGTT CCATAACAAG AAGCTATTAC AGAGGAGCGG CCGGCGCTCT TCTCGTTTAT GACGTCACGA ACAGGAGTAG CTTTGAAGCC CTTGAGGGTT GGCTTAAGGA CATGCTCGCC GGGGTGGGCA TGGAAGCCAG TCCGAACGGC GTTAGCGGCA TGGTTATTAT GTTAGTGGGA AACAAGACAG ATCTCGAGCA CAAGAGGAAA GTCTCGAAGG AAGAGGGCGA GCAGTTTGCC CGTGACCACG GCCTTCTCTT CATCGAAACC TCAGCAAAAG ACGACCTGAA CGTCTCGGAG AGCTTTAACG AGACGGCGCG GACCATTCTT GAGAAAGTGG AAACAGGGGA GATCGACTGC GTCTCTAAGG ATAGTGGGGT GAAGGCAGGG GCTCTCAATA TGAGCGCTGC AGCGACCACT GCCTCGTCCA CTGACAGCAC ATTTGGAAAT TGCTCCTGTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A