GilaA.01209.c

Kinase, NEK

CENTER ID: GilaA.01209.c
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.01209.c.B1.GE42271 full length 1 697
GilaA.01209.c.B4.GE42597 core kinase domain plus ordered region 15 286
GilaA.01209.c.HZ4.GE42598 core kinase domain plus ordered region 15 286
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_112518
UniProt: A8B9W0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHAASSEPAT TALSAPQFTP RELYEGLDDV LSESAAGTVY SLKDYPSLAV KVIQLSGLDK DSVDAAKAKL TVLLTLSYIG VLKHYQVIEY KGLIYVVTDR YNKTLEHLLA EHKRRKIPVP IAMILSVLRQ VAAALAYLHS VNGANTRGLV HCDLRPANVL ISADGERFVI ADFGLCKDAL WSGSTLIGIM AYVAPEVLLR NDTSPASDVW SLGVIIYELV TLRRPNFLGG KEPAEVFVDE WTPDLSGVAD GFIKNVLERI FVLEPKERLT ARELHETLTA ADIPVGELGH WYMALEDKCS ALETALNSTN ARITALEAAL ENRLSGADAL GQGPRLKTAR IDVLEYQCKK HLAMIKALEN RFTKFSSEMN RADSQTNLST LPQLIYAAHT NDMETIRILA NDEDCIGQRD EQKMTALMHA AQQGHIGPVK LLVEKEKGLK DNNGWTALMH AAHCNHSEIV EILAPYEHGR RNNNNRTALM IIAEEGHAEA ASVLVPYERD LIDSEGNTAL MIAGEARHEI VANILESTDK LGRTRLMRAA IRGCIETAKL VIKYDGGAQD IFGVTALMIA ASVGNVEIVK LLVGLEGGMR DGSETTALMN AASAGNVEIV KLLVDLEGGV KNMEEMTALE CGAVNGHLEV VKLLFEKEGH LIDKSDESFF GELENDGYSE IASFLRNSRT PGADDCNDHQ AAPRSHE
NT Sequence
atgcacgctg cctcgtctga gcctgcaaca acagctctca gtgccccgca gttcaccccc agggagctct acgaggggct ggatgatgtg cttagtgaaa gtgccgcggg aacagtctac tctctcaagg actatccgag tctcgctgta aaagtcatcc agctcagtgg cttggacaag gacagtgtag acgccgcaaa agccaaattg accgtcctcc ttaccctctc ctacatagga gttctcaagc actatcaggt gatcgagtac aagggcctca tctacgtcgt cacggaccgg tataacaaga cccttgagca cctcctcgcc gagcacaaac gaaggaagat ccccgtccct atcgctatga ttctctccgt cctgaggcag gtcgctgccg ccctcgccta cctccacagc gtcaatggag caaacactag aggcctcgtc cactgtgatc tcaggcctgc caatgtcctt atcagcgcag acggagagcg cttcgtcatc gccgacttcg gcctctgcaa ggacgccctg tggagcggga gcactctcat aggaataatg gcatacgtgg cccccgaggt gctcctccgc aacgatacca gccccgcctc cgacgtgtgg agcctcgggg tcatcattta tgaactagta actttgagga gaccgaactt cttggggggc aaggagccgg cagaggtctt cgttgacgag tggacaccgg acctgagcgg tgtcgctgat ggcttcataa agaatgttct ggagaggata ttcgtgctgg agccaaagga aagactgaca gccagggagc tgcacgagac gctcactgcg gctgacatcc cagttggtga gctggggcat tggtatatgg cactagaaga caaatgcagc gccttggaga ctgctttgaa cagtactaat gccaggatca ctgctcttga ggcagccctc gagaacaggt taagtggagc cgacgcccta ggacaggggc cccgacttaa gaccgccagg atcgacgtgc ttgaatatca atgcaagaaa catttagcca tgattaaggc actggagaat agattcacaa aatttagtag tgagatgaac agggctgact cccagaccaa cctctccacg ctgcctcaac tgatatacgc tgcacacacg aatgacatgg agaccatccg gatcctggcc aacgatgaag attgtatcgg ccagcgcgac gagcagaaga tgacggccct catgcacgcg gcccagcagg ggcacattgg tcctgtcaag ctgcttgttg agaaggagaa gggtctcaag gacaacaatg gctggactgc actcatgcac gccgcacatt gcaaccactc cgagatagtt gaaattctcg ccccttacga acatgggaga agaaataaca acaatcgcac tgctctgatg atcattgcag aggagggtca tgcagaagct gcctctgttc tagtcccata tgagagggac ttaatcgaca gcgaaggcaa cacggctctg atgattgcgg gggaggcgag gcacgaaatt gttgcgaata ttcttgagtc aaccgacaaa ctcggcagaa caaggcttat gcgagcggct atacgcggtt gcatagagac agcaaagttg gtaattaaat atgacggtgg tgcacaagac atattcggtg taactgcact tatgattgca gcttctgtgg gaaacgtaga aatagtaaaa ttgttagtag gtctggaggg gggtatgaga gacggatcag aaacgactgc acttatgaac gcggcttctg cgggaaacgt agaaatagta aaattgttag tagatctgga ggggggtgta aagaatatgg aggagatgac tgcccttgaa tgtggggcgg taaatggcca cttagaggta gtgaagctgc tatttgaaaa ggaggggcat ctaatagaca agtctgacga atccttcttt ggtgagcttg agaatgatgg ctactctgag atagcatcct tccttaggaa cagccgtaca ccgggcgccg acgactgcaa cgatcaccaa gctgctccac gatctcacga gtaa
Details for GilaA.01209.c.B1.GE42271
HARVESTED ON: 2/23/2018
SEQUENCED ON: 3/1/2018
EXPECTED MW: 77kDa
OBSERVED MW: 77kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMH AASSEPATTA LSAPQFTPRE LYEGLDDVLS ESAAGTVYSL KDYPSLAVKV IQLSGLDKDS VDAAKAKLTV LLTLSYIGVL KHYQVIEYKG LIYVVTDRYN KTLEHLLAEH KRRKIPVPIA MILSVLRQVA AALAYLHSVN GANTRGLVHC DLRPANVLIS ADGERFVIAD FGLCKDALWS GSTLIGIMAY VAPEVLLRND TSPASDVWSL GVIIYELVTL RRPNFLGGKE PAEVFVDEWT PDLSGVADGF IKNVLERIFV LEPKERLTAR ELHETLTAAD IPVGELGHWY MALEDKCSAL ETALNSTNAR ITALEAALEN RLSGADALGQ GPRLKTARID VLEYQCKKHL AMIKALENRF TKFSSEMNRA DSQTNLSTLP QLIYAAHTND METIRILAND EDCIGQRDEQ KMTALMHAAQ QGHIGPVKLL VEKEKGLKDN NGWTALMHAA HCNHSEIVEI LAPYEHGRRN NNNRTALMII AEEGHAEAAS VLVPYERDLI DSEGNTALMI AGEARHEIVA NILESTDKLG RTRLMRAAIR GCIETAKLVI KYDGGAQDIF GVTALMIAAS VGNVEIVKLL VGLEGGMRDG SETTALMNAA SAGNVEIVKL LVDLEGGVKN MEEMTALECG AVNGHLEVVK LLFEKEGHLI DKSDESFFGE LENDGYSEIA SFLRNSRTPG ADDCNDHQAA PRSHE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcacg ctgcctcgtc tgagcctgca acaacagctc tcagtgcccc gcagttcacc cccagggagc tctacgaggg gctggatgat gtgcttagtg aaagtgccgc gggaacagtc tactctctca aggactatcc gagtctcgct gtaaaagtca tccagctcag tggcttggac aaggacagtg tagacgccgc aaaagccaaa ttgaccgtcc tccttaccct ctcctacata ggagttctca agcactatca ggtgatcgag tacaagggcc tcatctacgt cgtcacggac cggtataaca agacccttga gcacctcctc gccgagcaca aacgaaggaa gatccccgtc cctatcgcta tgattctctc cgtcctgagg caggtcgctg ccgccctcgc ctacctccac agcgtcaatg gagcaaacac tagaggcctc gtccactgtg atctcaggcc tgccaatgtc cttatcagcg cagacggaga gcgcttcgtc atcgccgact tcggcctctg caaggacgcc ctgtggagcg ggagcactct cataggaata atggcatacg tggcccccga ggtgctcctc cgcaacgata ccagccccgc ctccgacgtg tggagcctcg gggtcatcat ttatgaacta gtaactttga ggagaccgaa cttcttgggg ggcaaggagc cggcagaggt cttcgttgac gagtggacac cggacctgag cggtgtcgct gatggcttca taaagaatgt tctggagagg atattcgtgc tggagccaaa ggaaagactg acagccaggg agctgcacga gacgctcact gcggctgaca tcccagttgg tgagctgggg cattggtata tggcactaga agacaaatgc agcgccttgg agactgcttt gaacagtact aatgccagga tcactgctct tgaggcagcc ctcgagaaca ggttaagtgg agccgacgcc ctaggacagg ggccccgact taagaccgcc aggatcgacg tgcttgaata tcaatgcaag aaacatttag ccatgattaa ggcactggag aatagattca caaaatttag tagtgagatg aacagggctg actcccagac caacctctcc acgctgcctc aactgatata cgctgcacac acgaatgaca tggagaccat ccggatcctg gccaacgatg aagattgtat cggccagcgc gacgagcaga agatgacggc cctcatgcac gcggcccagc aggggcacat tggtcctgtc aagctgcttg ttgagaagga gaagggtctc aaggacaaca atggctggac tgcactcatg cacgccgcac attgcaacca ctccgagata gttgaaattc tcgcccctta cgaacatggg agaagaaata acaacaatcg cactgctctg atgatcattg cagaggaggg tcatgcagaa gctgcctctg ttctagtccc atatgagagg gacttaatcg acagcgaagg caacacggct ctgatgattg cgggggaggc gaggcacgaa attgttgcga atattcttga gtcaaccgac aaactcggca gaacaaggct tatgcgagcg gctatacgcg gttgcataga gacagcaaag ttggtaatta aatatgacgg tggtgcacaa gacatattcg gtgtaactgc acttatgatt gcagcttctg tgggaaacgt agaaatagta aaattgttag taggtctgga ggggggtatg agagacggat cagaaacgac tgcacttatg aacgcggctt ctgcgggaaa cgtagaaata gtaaaattgt tagtagatct ggaggggggt gtaaagaata tggaggagat gactgccctt gaatgtgggg cggtaaatgg ccacttagag gtagtgaagc tgctatttga aaaggagggg catctaatag acaagtctga cgaatccttc tttggtgagc ttgagaatga tggctactct gagatagcat ccttccttag gaacagccgt acaccgggcg ccgacgactg caacgatcac caagctgctc cacgatctca cgagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for GilaA.01209.c.B4.GE42597
HARVESTED ON: 8/5/2018
SEQUENCED ON: 8/8/2018
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 69
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA PQFTIRDLHE WLDSVLNEST MGTVYSLEGY PNLAVKEILL DGLGKSNVDA IKLELATLPD LSHPGVLRHH QVVEDESFIY IVMNRHDKTL EQVFIDCKRR KIPVSAGVVL SILRQLSAAL AYLHGLSGAN TKGLVHRDLR PANIFISADG ERFIIADFGL CKDALRCGST IAGTAVYMAP EALLRNEASP ASDVWSLGVI IYELVTLRRP DFLGGKEPAE VFIDGWRPDL SGVTDGFMQN VLERIFVLEP ERRPTARELC KMLTAXDIPV G
Validated NT Sequence
atcttactca cttaaccaac tgggatgtca gnngcagtga gcatcttgca cagctccctg gccgtcggcc tcctctctgg ctccagcacg aatatcctct ccagaacatt ctgcatgaag ccatcagtga caccgctcag atcgggcctc cacccatcga tgaagacctc tgccggctcc ttgcccccca agaagtccgg cctcctcaga gttactagtt cataaatgat gaccccgagg ctccacacgt cggaggcggg gctggcctca ttacggagga gcgcctcggg ggccatgtat actgccgtgc ctgcaatggt gctcccgcat ctcagggcgt ccttgcagag gccgaagtca gcaatgatga agcgctctcc gtctgcgctg ataaatatat tggcaggcct gaggtcgcgg tggacgaggc ctttagtgtt tgccccactg aggccatgga ggtaggcgag ggcggcggag agctgtctca gaatggagag gaccacgccg gcagacacgg ggatcttcct ccgcttgcag tcgataaaca cttgctcaag ggtcttgtcg tggcgattca tgacgatgta aatgaagctt tcgtcttcga ctacttgatg gtgcctgaga acccctggat gggagaggtc ggggagggtg gccagctcaa gcttgatagc gtccacgttg ctcttgccca ggccgtcgag gaggatctcc ttcacggcga ggtttggata gccctcgagg gagtagactg tccccatggt gctttcatta agcacgctat ccagccactc gtggaggtcc ctgatggtga actgcggggc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHAP QFTPRELYEG LDDVLSESAA GTVYSLKDYP SLAVKVIQLS GLDKDSVDAA KAKLTVLLTL SYIGVLKHYQ VIEYKGLIYV VTDRYNKTLE HLLAEHKRRK IPVPIAMILS VLRQVAAALA YLHSVNGANT RGLVHCDLRP ANVLISADGE RFVIADFGLC KDALWSGSTL IGIMAYVAPE VLLRNDTSPA SDVWSLGVII YELVTLRRPN FLGGKEPAEV FVDEWTPDLS GVADGFIKNV LERIFVLEPK ERLTARELHE TLTAADIPVG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccccgc agttcacccc cagggagctc tacgaggggc tggatgatgt gcttagtgaa agtgccgcgg gaacagtcta ctctctcaag gactatccga gtctcgctgt aaaagtcatc cagctcagtg gcttggacaa ggacagtgta gacgccgcaa aagccaaatt gaccgtcctc cttaccctct cctacatagg agttctcaag cactatcagg tgatcgagta caagggcctc atctacgtcg tcacggaccg gtataacaag acccttgagc acctcctcgc cgagcacaaa cgaaggaaga tccccgtccc tatcgctatg attctctccg tcctgaggca ggtcgctgcc gccctcgcct acctccacag cgtcaatgga gcaaacacta gaggcctcgt ccactgtgat ctcaggcctg ccaatgtcct tatcagcgca gacggagagc gcttcgtcat cgccgacttc ggcctctgca aggacgccct gtggagcggg agcactctca taggaataat ggcatacgtg gcccccgagg tgctcctccg caacgatacc agccccgcct ccgacgtgtg gagcctcggg gtcatcattt atgaactagt aactttgagg agaccgaact tcttgggggg caaggagccg gcagaggtct tcgttgacga gtggacaccg gacctgagcg gtgtcgctga tggcttcata aagaatgttc tggagaggat attcgtgctg gagccaaagg aaagactgac agccagggag ctgcacgaga cgctcactgc ggctgacatc ccagttggtt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for GilaA.01209.c.HZ4.GE42598
HARVESTED ON: data unavailable
SEQUENCED ON: 8/8/2018
EXPECTED MW: 43kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 79
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMA PQFTPRELHE RLGDGLNENT MGTVYSLKGY PNLAVQEILL DGLDKGDLDT IRLELATLLD LSHPRVLKHH QVVEDEGLIY VVTDRHDRTL ERLLTEHKRR KIPVSAGVIL SVMKQLAAAL AYLHGLSGVG ANGLVHCDLR PANVLISADG ERFVIADFGL CRNTLWSEST IAGMAVYMAP EMLLHNEPSP ASDVWSLGVI LYEMATLRRP DFLKGKDPKD VFVDRWRPDL SSVTDGFIKS VLERIFVLEP EERLTARELH ETLTAADI
Validated NT Sequence
tgggatgtca gccgcagtga gcgtctcgtg cagctccctg gctgtcagtc tttcctctgg ctccagcacg aatatcctct ccagaacact ctttatgaag ccatcggtga cactgctcag gtccggcctc cacctgtcga cgaagacgtc ttttgggtct ttacctttca ggaagtccgg tctcctcaga gtggccatct cgtagaggat gaccccgagg ctccacacat cggaggcggg gctgggctcg ttgtggagga gcatctcggg ggccatgtat actgccatgc ctgcaatggt gctctcactc cacaatgtgt ttctgcagag gccgaagtcg gcgatgacga agcgctcccc gtctgcgctg atgaggacgt tggcaggcct gaggtcgcag tggacaagcc cattagcacc taccccgctg agaccatgaa gataggcgag ggcggcggcg agctgtttca tgacggagag gatcacgccg gcagacacgg ggatcttcct tcgtttgtgc tcggtgagga ggcgctcgag ggtcctgtcg tgccggtccg tgacgacgta gatgaggccc tcgtcctcga ctacttggtg gtgcttgagg accctcggat gggagaggtc gagaagggtg gccagctcta gtctgatggt gtccagatcc cccttgtcca ggccatcgag gaggatctcc tgcacagcga ggtttggata gcccttgaga gagtagactg tccccatggt gttttcatta agcccatcac ctaaccgctc atgaagctcc ctaggggtga actgcggggc catcgaacca ggaccctggg tctgagcttc cagggtacca ccaatctgtt ctctgtgagc ctcaataata tcgttatcct ccatgtccaa atcttcaggg gtctgatcag cttgaattct aataccgtcg tacaagaatc ttaaggagtc catttcctta ccctgtcttt tagcgaacgc ttccatcagc cttcttaaag gagtggtctt tttgatcttg aagaagatct ctgaagatcc atcggacacc tttaaattga tgtgagtctc aggcttgact tctggcttga cctctggctt agcttcttga ttgacttctg agtccgactt catatgatgg tgatggtgat gagccat
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSAP QFTPRELYEG LDDVLSESAA GTVYSLKDYP SLAVKVIQLS GLDKDSVDAA KAKLTVLLTL SYIGVLKHYQ VIEYKGLIYV VTDRYNKTLE HLLAEHKRRK IPVPIAMILS VLRQVAAALA YLHSVNGANT RGLVHCDLRP ANVLISADGE RFVIADFGLC KDALWSGSTL IGIMAYVAPE VLLRNDTSPA SDVWSLGVII YELVTLRRPN FLGGKEPAEV FVDEWTPDLS GVADGFIKNV LERIFVLEPK ERLTARELHE TLTAADIPVG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcgg ccccgcagtt cacccccagg gagctctacg aggggctgga tgatgtgctt agtgaaagtg ccgcgggaac agtctactct ctcaaggact atccgagtct cgctgtaaaa gtcatccagc tcagtggctt ggacaaggac agtgtagacg ccgcaaaagc caaattgacc gtcctcctta ccctctccta cataggagtt ctcaagcact atcaggtgat cgagtacaag ggcctcatct acgtcgtcac ggaccggtat aacaagaccc ttgagcacct cctcgccgag cacaaacgaa ggaagatccc cgtccctatc gctatgattc tctccgtcct gaggcaggtc gctgccgccc tcgcctacct ccacagcgtc aatggagcaa acactagagg cctcgtccac tgtgatctca ggcctgccaa tgtccttatc agcgcagacg gagagcgctt cgtcatcgcc gacttcggcc tctgcaagga cgccctgtgg agcgggagca ctctcatagg aataatggca tacgtggccc ccgaggtgct cctccgcaac gataccagcc ccgcctccga cgtgtggagc ctcggggtca tcatttatga actagtaact ttgaggagac cgaacttctt ggggggcaag gagccggcag aggtcttcgt tgacgagtgg acaccggacc tgagcggtgt cgctgatggc ttcataaaga atgttctgga gaggatattc gtgctggagc caaaggaaag actgacagcc agggagctgc acgagacgct cactgcggct gacatcccag ttggttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa