GilaA.01209.b

Kinase, NEK

CENTER ID: GilaA.01209.b
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.01209.b.B1.GE37529 full length 1 597
GilaA.01209.b.B2.GE37530 kinase domain 1 277
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_8445
RefSeq: XP_001704268.1
UniProt: A8BXP9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGIAEKLAEY RELMAIKRGH YGIVHKVEHI STKKIYARKT VKYGKLDQRG VELIMREANL LSRMKHINVL GYHDVVIDEV EKVIYIFTEF CNKGDLFQLI DKHRYPRTNI PEERIWKLFG QLLLALEYCH CPTKENFELG EIVIHRDIKP ANILITNDDV VKLADFGFSR SFNSSDMLKT QLGTPSYTAP EVLNKTPYNE KADIWSLGCV IFHLCSLEFP FQAMTHADLA RNVRDGKRRP FPQGIYSKEL EELVDSMMIV DYTQRPSAAQ LIRHRKFIEH GVITADYLNT NPTEEIDDKA KMIAELKMCL KMKQQEVDNL EDDLSNNKEI MEAMTITKTA SAPSGTATSS AESNNDAVEK LRAELSLQSM RVKQLEAENS ELRSQNTILT NKILKLEEAA RRVAQAQSAI ASDGMTALMR AVQMNDIAGI KAHIATDAGK KNRKGKTALM MCSDTGKTEA AALLIPYEAG TKMSDGTTAL ILAAIAGHVD IVNILKKKEG KMQARQGDTA LINAAVTGHA EVVRALVEIE AGLRMDDGRA AIHNAAYKGH LECVKILLPY EYHMRDKSGL SPLKYASKGK QEAVVDYIRT WLQTNNK
NT Sequence
atgggtattg cggagaagct ggcagaatat cgagagctta tggccatcaa gcggggccat tatgggatag tgcacaaggt ggagcatatc agcacgaaaa aaatatatgc tagaaaaacc gtcaaatacg ggaaactgga ccagcgaggg gttgagctca taatgcgtga agctaacctt cttagccgca tgaagcatat aaatgtacta ggatatcacg acgtggtgat agatgaagta gagaaagtca tctacatctt taccgagttc tgtaataagg gtgatctttt ccaattaatc gacaagcacc gatatcctcg gacaaatatc cccgaagagc gcatatggaa gctcttcgga caacttctac tggcactgga atactgtcat tgccccacta aagagaactt tgagcttggc gagatcgtta ttcataggga catcaagccg gccaatattt tgataacgaa tgatgatgtg gtaaagcttg ccgactttgg attctctcgg tcgtttaatt cctcagacat gctcaaaacc caattgggta cgccgagcta cactgcacca gaggtcctca acaaaacgcc ctacaatgag aaggcagata tctggtctct agggtgcgtt atattccact tatgcagcct ggagtttcca ttccaggcca tgacccacgc agacctggca cgcaatgtcc gcgatggaaa gcgacgccca tttccacaag gcatatacag caaggagctc gaggaactgg tggattcgat gatgattgtt gattataccc agaggcctag tgctgcccag ttaattcgtc accggaaatt catcgagcac ggggtcatca cggccgacta tctcaataca aaccccaccg aggagattga cgacaaggct aagatgattg ccgagctgaa gatgtgtcta aagatgaagc agcaagaggt cgacaacctg gaagacgatt tatcaaacaa taaggagata atggaagcaa tgacgattac gaagactgca agtgcgccct ctgggactgc cacgtcaagc gcagagagca ataacgatgc agtagaaaag cttcgtgcag aacttagttt acaatcaatg cgggtaaagc aattagaagc agagaatagc gaacttcgtt cgcaaaacac cattttgaca aataaaattc tcaaattaga agaggctgca aggcgggtcg ctcaggccca atctgcaatc gcgtcagatg gtatgacagc tctgatgcgt gcagttcaaa tgaatgacat tgctggaatc aaggctcata tagcaacaga tgcagggaaa aagaaccgca aagggaaaac tgccttaatg atgtgtagcg ataccggaaa aactgaagct gcggcacttc ttattccgta tgaggccggg actaagatgt ccgacggcac tacagctttg attctcgcag caatagcggg tcacgttgac attgttaata ttctcaaaaa gaaggaagga aagatgcagg ctagacaggg ggacactgcc ctgatcaacg ctgctgttac tgggcatgca gaggttgtgc gcgcccttgt agaaatcgaa gccggtctcc gcatggacga tgggcgtgca gccatccaca atgccgctta taagggccat ctcgaatgtg tcaagatcct gctgccatac gagtaccaca tgcgcgataa atctggcctg agtcccctca agtacgctag caaaggaaag caggaggctg ttgttgatta tataagaaca tggctacaga ctaacaacaa gtag
Details for GilaA.01209.b.B1.GE37529
HARVESTED ON: data unavailable
SEQUENCED ON: 12/4/2013
EXPECTED MW: 68kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Data Unavailable
SOLUBLE EXPRESSION LEVEL Data Unavailable
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 51
Validated AA Sequence
MAHHHHHHHM GIAEKLAEYR ELMAIKRGHY GIVHKVEHIS TKKIYARKTV KYGKLDQRGV ELIMREANLL SRMKHINVLG YHDVVIDEVE KVIYIFTEFC NKGDLFQLID KHRYPRTNIP EERIWKLFGQ LLLALEYCHC PTKENFELGE IVIHRDIKPA NILITNDDVV KLADFGFSRS FNSSDMLKTQ LGTPSYTAPE VLNKTPYNEK ADIWSLGCVI FHLCSLEFPF QAMTHADLAR NVRDGKRRPF PQGIYSKELE ELVDSMMIVD YTQRPSAAQL IRHRKFIEHG VITADYLNTN PTEEIDDXLR
Validated NT Sequence
aatattttgt ttaactttaa gaaggagata taccatggct caccaccacc accaccacca tatgggtatt gcggagaagc tggcagaata tcgagagctt atggccatca agcggggcca ttatgggata gtgcacaagg tggagcatat cagcacgaaa aaaatatatg ctagaaaaac cgtcaaatac gggaaactgg accagcgagg ggttgagctc ataatgcgtg aagctaacct tcttagccgc atgaagcata taaatgtact aggatatcac gacgtggtga tagatgaagt agagaaagtc atctacatct ttaccgagtt ctgtaataag ggtgatcttt tccaattaat cgacaagcac cgatatcctc ggacaaatat ccccgaagag cgcatatgga agctcttcgg acaacttcta ctggcactgg aatactgtca ttgccccact aaagagaact ttgagcttgg cgagatcgtt attcataggg acatcaagcc ggccaatatt ttgataacga atgatgatgt ggtaaagctt gccgactttg gattctctcg gtcgtttaat tcctcagaca tgctcaaaac ccaattgggt acgccgagct acactgcacc agaggtcctc aacaaaacgc cctacaatga gaaggcagat atctggtctc tagggtgcgt tatattccac ttatgcagcc tggagtttcc attccaggcc atgacccacg cagacctggc acgcaatgtc cgcgatggaa agcgacgccc atttccacaa ggcatataca gcaaggagct cgaggaactg gtggattcga tgatgattgt tgattatacc cagaggccta gtgctgccca gttaattcgt caccggaaat tcatcgagca cggggtcatc acggccgact atctcaatac aaaccccacc gaggagattg acgacangct aagatgattg ccgagctgaa gatgtgtcta aagatgaagc agcagaggtc gacanctggn nacgatttat caaacataga gananggnnc atgacgatta cganactgca gtgcgccctc tgggactgcn cgtcagcgca nagagcatac gatgcagtag aaaagctnnn cagactannn natcangnng gnnnnnnnna ncnnnnnann gcgaantcnn nnncnannan nnnnnnttnn nngnnncagn nggnnnnnnn nnnnantc
Expected Protein Sequence
MAHHHHHHMG IAEKLAEYRE LMAIKRGHYG IVHKVEHIST KKIYARKTVK YGKLDQRGVE LIMREANLLS RMKHINVLGY HDVVIDEVEK VIYIFTEFCN KGDLFQLIDK HRYPRTNIPE ERIWKLFGQL LLALEYCHCP TKENFELGEI VIHRDIKPAN ILITNDDVVK LADFGFSRSF NSSDMLKTQL GTPSYTAPEV LNKTPYNEKA DIWSLGCVIF HLCSLEFPFQ AMTHADLARN VRDGKRRPFP QGIYSKELEE LVDSMMIVDY TQRPSAAQLI RHRKFIEHGV ITADYLNTNP TEEIDDKAKM IAELKMCLKM KQQEVDNLED DLSNNKEIME AMTITKTASA PSGTATSSAE SNNDAVEKLR AELSLQSMRV KQLEAENSEL RSQNTILTNK ILKLEEAARR VAQAQSAIAS DGMTALMRAV QMNDIAGIKA HIATDAGKKN RKGKTALMMC SDTGKTEAAA LLIPYEAGTK MSDGTTALIL AAIAGHVDIV NILKKKEGKM QARQGDTALI NAAVTGHAEV VRALVEIEAG LRMDDGRAAI HNAAYKGHLE CVKILLPYEY HMRDKSGLSP LKYASKGKQE AVVDYIRTWL QTNNK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggta ttgcggagaa gctggcagaa tatcgagagc ttatggccat caagcggggc cattatggga tagtgcacaa ggtggagcat atcagcacga aaaaaatata tgctagaaaa accgtcaaat acgggaaact ggaccagcga ggggttgagc tcataatgcg tgaagctaac cttcttagcc gcatgaagca tataaatgta ctaggatatc acgacgtggt gatagatgaa gtagagaaag tcatctacat ctttaccgag ttctgtaata agggtgatct tttccaatta atcgacaagc accgatatcc tcggacaaat atccccgaag agcgcatatg gaagctcttc ggacaacttc tactggcact ggaatactgt cattgcccca ctaaagagaa ctttgagctt ggcgagatcg ttattcatag ggacatcaag ccggccaata ttttgataac gaatgatgat gtggtaaagc ttgccgactt tggattctct cggtcgttta attcctcaga catgctcaaa acccaattgg gtacgccgag ctacactgca ccagaggtcc tcaacaaaac gccctacaat gagaaggcag atatctggtc tctagggtgc gttatattcc acttatgcag cctggagttt ccattccagg ccatgaccca cgcagacctg gcacgcaatg tccgcgatgg aaagcgacgc ccatttccac aaggcatata cagcaaggag ctcgaggaac tggtggattc gatgatgatt gttgattata cccagaggcc tagtgctgcc cagttaattc gtcaccggaa attcatcgag cacggggtca tcacggccga ctatctcaat acaaacccca ccgaggagat tgacgacaag gctaagatga ttgccgagct gaagatgtgt ctaaagatga agcagcaaga ggtcgacaac ctggaagacg atttatcaaa caataaggag ataatggaag caatgacgat tacgaagact gcaagtgcgc cctctgggac tgccacgtca agcgcagaga gcaataacga tgcagtagaa aagcttcgtg cagaacttag tttacaatca atgcgggtaa agcaattaga agcagagaat agcgaacttc gttcgcaaaa caccattttg acaaataaaa ttctcaaatt agaagaggct gcaaggcggg tcgctcaggc ccaatctgca atcgcgtcag atggtatgac agctctgatg cgtgcagttc aaatgaatga cattgctgga atcaaggctc atatagcaac agatgcaggg aaaaagaacc gcaaagggaa aactgcctta atgatgtgta gcgataccgg aaaaactgaa gctgcggcac ttcttattcc gtatgaggcc gggactaaga tgtccgacgg cactacagct ttgattctcg cagcaatagc gggtcacgtt gacattgtta atattctcaa aaagaaggaa ggaaagatgc aggctagaca gggggacact gccctgatca acgctgctgt tactgggcat gcagaggttg tgcgcgccct tgtagaaatc gaagccggtc tccgcatgga cgatgggcgt gcagccatcc acaatgccgc ttataagggc catctcgaat gtgtcaagat cctgctgcca tacgagtacc acatgcgcga taaatctggc ctgagtcccc tcaagtacgc tagcaaagga aagcaggagg ctgttgttga ttatataaga acatggctac agactaacaa caagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for GilaA.01209.b.B2.GE37530
HARVESTED ON: 11/25/2013
SEQUENCED ON: 12/4/2013
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: failed alignment
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG IAEKLAEYRE LMAIKRGHYG IVHKVEHIST KKIYARKTVK YGKLDQRGVE LIMREANLLS RMKHINVLGY HDVVIDEVEK VIYIFTEFCN KGDLFQLIDK HRYPRTNIPE ERIWKLFGQL LLALEYCHCP TKENFELGEI VIHRDIKPAN ILITNDDVVK LADFGFSRSF NSSDMLKTQL GTPSYTAPEV LNKTPYNEKA DIWSLGCVIF HLCSLEFPFQ AMTHADLARN VRDGKRRPFP QGIYSKELEE LVDSMMIVDY TQRPSAAQLI RHRKF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggta ttgcggagaa gctggcagaa tatcgagagc ttatggccat caagcggggc cattatggga tagtgcacaa ggtggagcat atcagcacga aaaaaatata tgctagaaaa accgtcaaat acgggaaact ggaccagcga ggggttgagc tcataatgcg tgaagctaac cttcttagcc gcatgaagca tataaatgta ctaggatatc acgacgtggt gatagatgaa gtagagaaag tcatctacat ctttaccgag ttctgtaata agggtgatct tttccaatta atcgacaagc accgatatcc tcggacaaat atccccgaag agcgcatatg gaagctcttc ggacaacttc tactggcact ggaatactgt cattgcccca ctaaagagaa ctttgagctt ggcgagatcg ttattcatag ggacatcaag ccggccaata ttttgataac gaatgatgat gtggtaaagc ttgccgactt tggattctct cggtcgttta attcctcaga catgctcaaa acccaattgg gtacgccgag ctacactgca ccagaggtcc tcaacaaaac gccctacaat gagaaggcag atatctggtc tctagggtgc gttatattcc acttatgcag cctggagttt ccattccagg ccatgaccca cgcagacctg gcacgcaatg tccgcgatgg aaagcgacgc ccatttccac aaggcatata cagcaaggag ctcgaggaac tggtggattc gatgatgatt gttgattata cccagaggcc tagtgctgcc cagttaattc gtcaccggaa attctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat