GilaA.01209.a

Kinase, CAMK CAMKL

CENTER ID: GilaA.01209.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.01209.a.A1.GE25729 Full length( GilaA.01209.a ) 1 469
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.01209.a.A1.PS00171a Full length( GilaA.01209.a ) 1 469
External Resources
RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_14661
OrthoMCL: OG5_168484
RefSeq: XP_001706287.1
UniProt: A8BLF9
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDEQPVQLG QYEVSELLGS GAFAKVRLAT RKDTGKKYAV KIIKKVGLSQ RAADRLNLEI AVTKGISHPN VIKMHEVIGT SSKFWLVMDY HANGELLKHM KTKLSDDQAF DYFIQIACAL RYCHRFGICH RDLKFENVLV ANDGHLVITD FGLGAISYNP DKTFYSQATF CGSTHYIAPE VSSGSRYDGR KSDIWSLGIM LYAMLIGKLP FPSDNYEVLV ESMLNRRYDP IPSFVNDQAK NLLEALLEPD PRTRPDWREI FNYPWIASRV ALVDEAGHTV DKFPSEITDT GVLCRVMRKK KVHMEIPLHP NKKSYSVLSN IHAALMQTNS APSTAQPVSD EGFTCPSCGN IVVPPKTADI ASYSVKKAIM ISDISISDAL ILAAYGVRKV TKKPVVVYPH EFRASSDVQL MEARVFGTQK LVILIGTNDA EMYTTVSTVL TNLAADKAKL SAITLGRIND VSVVCEDAR
NT Sequence
ATGAGCGATG AGCAGCCAGT TCAGCTCGGA CAGTATGAGG TTTCGGAGCT GTTAGGGTCA GGGGCTTTTG CAAAGGTGCG CTTGGCAACC AGGAAGGATA CGGGGAAGAA GTATGCAGTG AAGATCATCA AGAAAGTGGG GCTATCTCAA AGAGCTGCCG ACCGCCTAAA CCTTGAGATT GCTGTCACAA AGGGCATTTC ACACCCAAAC GTCATAAAGA TGCACGAAGT CATCGGGACG TCTTCAAAGT TCTGGCTGGT GATGGACTAC CATGCCAACG GGGAGCTTCT AAAACACATG AAGACAAAAT TGTCAGACGA TCAGGCATTT GACTACTTTA TCCAGATAGC GTGCGCTCTT CGGTACTGTC ACAGATTCGG GATTTGTCAC CGAGACCTAA AGTTTGAAAA TGTCCTGGTG GCGAATGATG GGCATCTCGT GATAACTGAC TTCGGTCTGG GAGCTATTTC TTACAACCCT GACAAGACCT TTTACTCACA GGCAACCTTC TGCGGCTCAA CCCATTACAT TGCTCCAGAG GTATCCTCTG GAAGCAGGTA CGATGGGCGC AAATCGGATA TCTGGTCCTT GGGAATCATG CTCTATGCCA TGCTGATCGG CAAGCTGCCT TTCCCATCAG ATAACTACGA GGTCCTTGTG GAGTCCATGC TTAATCGTCG CTATGACCCT ATCCCTTCCT TTGTTAACGA CCAGGCCAAG AACTTGCTGG AGGCGCTGCT GGAACCAGAT CCTCGGACGA GACCCGACTG GAGGGAAATA TTCAATTACC CGTGGATTGC ATCGCGAGTC GCACTAGTGG ATGAAGCTGG GCATACGGTG GACAAGTTCC CTAGTGAGAT TACTGATACG GGTGTGCTCT GCAGAGTCAT GCGAAAGAAG AAGGTGCATA TGGAGATACC CCTTCATCCA AACAAGAAGT CGTACTCTGT CCTCTCCAAC ATACATGCTG CCTTAATGCA AACAAACAGC GCCCCAAGTA CGGCTCAGCC TGTTTCTGAT GAGGGATTCA CCTGCCCCAG CTGTGGAAAC ATTGTGGTAC CCCCTAAAAC CGCCGATATA GCCAGCTACT CTGTCAAGAA GGCCATCATG ATTTCAGACA TAAGCATATC AGATGCGCTG ATTCTAGCTG CGTACGGTGT CAGAAAGGTG ACGAAGAAAC CGGTTGTGGT CTATCCTCAC GAGTTTCGAG CAAGCTCGGA CGTTCAGCTG ATGGAAGCCC GCGTGTTCGG CACTCAGAAA CTGGTCATCC TTATTGGTAC TAACGACGCG GAGATGTACA CGACGGTCAG TACTGTTCTG ACAAACTTGG CAGCAGATAA GGCAAAGTTG TCTGCCATTA CCCTAGGCCG AATTAATGAC GTCTCAGTTG TTTGCGAAGA TGCACGG
Details for GilaA.01209.a.A1.GE25729
HARVESTED ON: 10/31/2008
SEQUENCED ON: 7/20/2010
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MSDEQPVQLG QYEVSELLGS GAFAKVRLAT RKDTGKKYAV KIIKKVGLSQ RAADRLNLEI AVTKGISHPN VIKMHEVIGT SSKFWLVMDY HANGELLKHM KTKLSDDQAF DYFIQIACAL RYCHRFGICH RDLKFENVLV ANDGHLVITD FGLGAISYNP DKTFYSQATF CGSTHYIAPE VSSGSRYDGR KSDIWSLGIM LYAMLIGKLP FPSDNYEVLV ESMLNRRYDP IPSFVNDQAK NLLEALLEPD PRTRPDWREI FNYPWIASRV ALVDEAGHTV DKFPSEITDT GVLCRVMRKK KVHMEIPLHP NKKSYSVLSN IHAALMQTNS APSTAQPVSD EGFTCPSCGN IVVPPKTADI ASYSVKKAIM ISDISISDAL ILAAYGVRKV TKKPVVVYPH EFRASSDVQL MEARVFGTQK LVILIGTNDA EMYTTVSTVL TNLAADKAKL SAITLGRIND VSVVCEDAR
Validated NT Sequence
accctggaag ctcagaccca gggtcctggt tcgatgagcg atgagcagcc agttcagctc ggacagtatg aggtttcgga gctgttaggg tcaggggctt ttgcaaaggt gcgcttggca accaggaagg atacggggaa gaagtatgca gtgaagatca tcaagaaagt ggggctatct caaagagctg ccgaccgcct aaaccttgag attgctgtca caaagggcat ttcacaccca aacgtcataa agatgcacga agtcatcggg acgtcttcaa agttctggct ggtgatggac taccatgcca acggggagct tctaaaacac atgaagacaa aattgtcaga cgatcaggca tttgactact ttatccagat agcgtgcgct cttcggtact gtcacagatt cgggatttgt caccgagacc taaagtttga aaatgtcctg gtggcgaatg atgggcatct cgtgataact gacttcggtc tgggagctat ttcttacaac cctgacaaga ccttttactc acaggcaacc ttctgcggct caacccatta cattgctcca gaggtatcct ctggaagcag gtacgatggg cgcaaatcgg atatctggtc cttgggaatc atgctctatg ccatgctgat cggcaagctg cctttcccat cagataacta cgaggtcctt gtggagtcca tgcttaatcg tcgctatgac cctatccctt cctttgttaa cgaccaggcc aagaacttgc tggaggcgct gctggaacca gatcctcgga cgagacccga ctggagggaa atattcaatt acccgtggat tgcatcgcga gtcgcactag tggatgaagc tgggcatacg gtggacaagt tccctagtga gattactgat acgggtgtgc tctgcagagt catgcgaaag aagaaggtgc atatggagat accccttcat ccaaacaaga agtcgtactc tgtcctctcc aacatacatg ctgccttaat gcaaacaaac agcgccccaa gtacggctca gcctgtttct gatgagggat tcacctgccc cagctgtgga aacattgtgg taccccctaa aaccgccgat atagccagct actctgtcaa gaaggccatc atgatttcag acataagcat atcagatgcg ctgattctag ctgcgtacgg tgtcagaaag gtgacgaaga aaccggttgt ggtctatcct cacgagtttc gagcaagctc ggacgttcag ctgatggaag cccgcgtgtt cggcactcag aaactggtca tccttattgg tactaacgac gcggagatgt acacgacggt cagtactgtt ctgacaaact tggcagcaga taaggcaaag ttgtctgcca ttaccctagg ccgaattaat gacgtctcag ttgtttgcga agatgcacgg t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDEQPVQL GQYEVSELLG SGAFAKVRLA TRKDTGKKYA VKIIKKVGLS QRAADRLNLE IAVTKGISHP NVIKMHEVIG TSSKFWLVMD YHANGELLKH MKTKLSDDQA FDYFIQIACA LRYCHRFGIC HRDLKFENVL VANDGHLVIT DFGLGAISYN PDKTFYSQAT FCGSTHYIAP EVSSGSRYDG RKSDIWSLGI MLYAMLIGKL PFPSDNYEVL VESMLNRRYD PIPSFVNDQA KNLLEALLEP DPRTRPDWRE IFNYPWIASR VALVDEAGHT VDKFPSEITD TGVLCRVMRK KKVHMEIPLH PNKKSYSVLS NIHAALMQTN SAPSTAQPVS DEGFTCPSCG NIVVPPKTAD IASYSVKKAI MISDISISDA LILAAYGVRK VTKKPVVVYP HEFRASSDVQ LMEARVFGTQ KLVILIGTND AEMYTTVSTV LTNLAADKAK LSAITLGRIN DVSVVCEDAR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgatgag cagccagttc agctcggaca gtatgaggtt tcggagctgt tagggtcagg ggcttttgca aaggtgcgct tggcaaccag gaaggatacg gggaagaagt atgcagtgaa gatcatcaag aaagtggggc tatctcaaag agctgccgac cgcctaaacc ttgagattgc tgtcacaaag ggcatttcac acccaaacgt cataaagatg cacgaagtca tcgggacgtc ttcaaagttc tggctggtga tggactacca tgccaacggg gagcttctaa aacacatgaa gacaaaattg tcagacgatc aggcatttga ctactttatc cagatagcgt gcgctcttcg gtactgtcac agattcggga tttgtcaccg agacctaaag tttgaaaatg tcctggtggc gaatgatggg catctcgtga taactgactt cggtctggga gctatttctt acaaccctga caagaccttt tactcacagg caaccttctg cggctcaacc cattacattg ctccagaggt atcctctgga agcaggtacg atgggcgcaa atcggatatc tggtccttgg gaatcatgct ctatgccatg ctgatcggca agctgccttt cccatcagat aactacgagg tccttgtgga gtccatgctt aatcgtcgct atgaccctat cccttccttt gttaacgacc aggccaagaa cttgctggag gcgctgctgg aaccagatcc tcggacgaga cccgactgga gggaaatatt caattacccg tggattgcat cgcgagtcgc actagtggat gaagctgggc atacggtgga caagttccct agtgagatta ctgatacggg tgtgctctgc agagtcatgc gaaagaagaa ggtgcatatg gagatacccc ttcatccaaa caagaagtcg tactctgtcc tctccaacat acatgctgcc ttaatgcaaa caaacagcgc cccaagtacg gctcagcctg tttctgatga gggattcacc tgccccagct gtggaaacat tgtggtaccc cctaaaaccg ccgatatagc cagctactct gtcaagaagg ccatcatgat ttcagacata agcatatcag atgcgctgat tctagctgcg tacggtgtca gaaaggtgac gaagaaaccg gttgtggtct atcctcacga gtttcgagca agctcggacg ttcagctgat ggaagcccgc gtgttcggca ctcagaaact ggtcatcctt attggtacta acgacgcgga gatgtacacg acggtcagta ctgttctgac aaacttggca gcagataagg caaagttgtc tgccattacc ctaggccgaa ttaatgacgt ctcagttgtt tgcgaagatg cacggaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for GilaA.01209.a.A1.PS00171a
PURIFICATION DATe: 1/27/2009
CONCENTRATION: 29.8mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MSDEQPVQLG QYEVSELLGS GAFAKVRLAT RKDTGKKYAV KIIKKVGLSQ RAADRLNLEI AVTKGISHPN VIKMHEVIGT SSKFWLVMDY HANGELLKHM KTKLSDDQAF DYFIQIACAL RYCHRFGICH RDLKFENVLV ANDGHLVITD FGLGAISYNP DKTFYSQATF CGSTHYIAPE VSSGSRYDGR KSDIWSLGIM LYAMLIGKLP FPSDNYEVLV ESMLNRRYDP IPSFVNDQAK NLLEALLEPD PRTRPDWREI FNYPWIASRV ALVDEAGHTV DKFPSEITDT GVLCRVMRKK KVHMEIPLHP NKKSYSVLSN IHAALMQTNS APSTAQPVSD EGFTCPSCGN IVVPPKTADI ASYSVKKAIM ISDISISDAL ILAAYGVRKV TKKPVVVYPH EFRASSDVQL MEARVFGTQK LVILIGTNDA EMYTTVSTVL TNLAADKAKL SAITLGRIND VSVVCEDAR
Validated NT Sequence
accctggaag ctcagaccca gggtcctggt tcgatgagcg atgagcagcc agttcagctc ggacagtatg aggtttcgga gctgttaggg tcaggggctt ttgcaaaggt gcgcttggca accaggaagg atacggggaa gaagtatgca gtgaagatca tcaagaaagt ggggctatct caaagagctg ccgaccgcct aaaccttgag attgctgtca caaagggcat ttcacaccca aacgtcataa agatgcacga agtcatcggg acgtcttcaa agttctggct ggtgatggac taccatgcca acggggagct tctaaaacac atgaagacaa aattgtcaga cgatcaggca tttgactact ttatccagat agcgtgcgct cttcggtact gtcacagatt cgggatttgt caccgagacc taaagtttga aaatgtcctg gtggcgaatg atgggcatct cgtgataact gacttcggtc tgggagctat ttcttacaac cctgacaaga ccttttactc acaggcaacc ttctgcggct caacccatta cattgctcca gaggtatcct ctggaagcag gtacgatggg cgcaaatcgg atatctggtc cttgggaatc atgctctatg ccatgctgat cggcaagctg cctttcccat cagataacta cgaggtcctt gtggagtcca tgcttaatcg tcgctatgac cctatccctt cctttgttaa cgaccaggcc aagaacttgc tggaggcgct gctggaacca gatcctcgga cgagacccga ctggagggaa atattcaatt acccgtggat tgcatcgcga gtcgcactag tggatgaagc tgggcatacg gtggacaagt tccctagtga gattactgat acgggtgtgc tctgcagagt catgcgaaag aagaaggtgc atatggagat accccttcat ccaaacaaga agtcgtactc tgtcctctcc aacatacatg ctgccttaat gcaaacaaac agcgccccaa gtacggctca gcctgtttct gatgagggat tcacctgccc cagctgtgga aacattgtgg taccccctaa aaccgccgat atagccagct actctgtcaa gaaggccatc atgatttcag acataagcat atcagatgcg ctgattctag ctgcgtacgg tgtcagaaag gtgacgaaga aaccggttgt ggtctatcct cacgagtttc gagcaagctc ggacgttcag ctgatggaag cccgcgtgtt cggcactcag aaactggtca tccttattgg tactaacgac gcggagatgt acacgacggt cagtactgtt ctgacaaact tggcagcaga taaggcaaag ttgtctgcca ttaccctagg ccgaattaat gacgtctcag ttgtttgcga agatgcacgg t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDEQPVQL GQYEVSELLG SGAFAKVRLA TRKDTGKKYA VKIIKKVGLS QRAADRLNLE IAVTKGISHP NVIKMHEVIG TSSKFWLVMD YHANGELLKH MKTKLSDDQA FDYFIQIACA LRYCHRFGIC HRDLKFENVL VANDGHLVIT DFGLGAISYN PDKTFYSQAT FCGSTHYIAP EVSSGSRYDG RKSDIWSLGI MLYAMLIGKL PFPSDNYEVL VESMLNRRYD PIPSFVNDQA KNLLEALLEP DPRTRPDWRE IFNYPWIASR VALVDEAGHT VDKFPSEITD TGVLCRVMRK KKVHMEIPLH PNKKSYSVLS NIHAALMQTN SAPSTAQPVS DEGFTCPSCG NIVVPPKTAD IASYSVKKAI MISDISISDA LILAAYGVRK VTKKPVVVYP HEFRASSDVQ LMEARVFGTQ KLVILIGTND AEMYTTVSTV LTNLAADKAK LSAITLGRIN DVSVVCEDAR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGATGAG CAGCCAGTTC AGCTCGGACA GTATGAGGTT TCGGAGCTGT TAGGGTCAGG GGCTTTTGCA AAGGTGCGCT TGGCAACCAG GAAGGATACG GGGAAGAAGT ATGCAGTGAA GATCATCAAG AAAGTGGGGC TATCTCAAAG AGCTGCCGAC CGCCTAAACC TTGAGATTGC TGTCACAAAG GGCATTTCAC ACCCAAACGT CATAAAGATG CACGAAGTCA TCGGGACGTC TTCAAAGTTC TGGCTGGTGA TGGACTACCA TGCCAACGGG GAGCTTCTAA AACACATGAA GACAAAATTG TCAGACGATC AGGCATTTGA CTACTTTATC CAGATAGCGT GCGCTCTTCG GTACTGTCAC AGATTCGGGA TTTGTCACCG AGACCTAAAG TTTGAAAATG TCCTGGTGGC GAATGATGGG CATCTCGTGA TAACTGACTT CGGTCTGGGA GCTATTTCTT ACAACCCTGA CAAGACCTTT TACTCACAGG CAACCTTCTG CGGCTCAACC CATTACATTG CTCCAGAGGT ATCCTCTGGA AGCAGGTACG ATGGGCGCAA ATCGGATATC TGGTCCTTGG GAATCATGCT CTATGCCATG CTGATCGGCA AGCTGCCTTT CCCATCAGAT AACTACGAGG TCCTTGTGGA GTCCATGCTT AATCGTCGCT ATGACCCTAT CCCTTCCTTT GTTAACGACC AGGCCAAGAA CTTGCTGGAG GCGCTGCTGG AACCAGATCC TCGGACGAGA CCCGACTGGA GGGAAATATT CAATTACCCG TGGATTGCAT CGCGAGTCGC ACTAGTGGAT GAAGCTGGGC ATACGGTGGA CAAGTTCCCT AGTGAGATTA CTGATACGGG TGTGCTCTGC AGAGTCATGC GAAAGAAGAA GGTGCATATG GAGATACCCC TTCATCCAAA CAAGAAGTCG TACTCTGTCC TCTCCAACAT ACATGCTGCC TTAATGCAAA CAAACAGCGC CCCAAGTACG GCTCAGCCTG TTTCTGATGA GGGATTCACC TGCCCCAGCT GTGGAAACAT TGTGGTACCC CCTAAAACCG CCGATATAGC CAGCTACTCT GTCAAGAAGG CCATCATGAT TTCAGACATA AGCATATCAG ATGCGCTGAT TCTAGCTGCG TACGGTGTCA GAAAGGTGAC GAAGAAACCG GTTGTGGTCT ATCCTCACGA GTTTCGAGCA AGCTCGGACG TTCAGCTGAT GGAAGCCCGC GTGTTCGGCA CTCAGAAACT GGTCATCCTT ATTGGTACTA ACGACGCGGA GATGTACACG ACGGTCAGTA CTGTTCTGAC AAACTTGGCA GCAGATAAGG CAAAGTTGTC TGCCATTACC CTAGGCCGAA TTAATGACGT CTCAGTTGTT TGCGAAGATG CACGGAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA