GilaA.01193.a

Kinase, NEK

CENTER ID: GilaA.01193.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.01193.a.A1.GE25728 Full length( GilaA.01193.a ) 1 367
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_11311
OrthoMCL: OG5_129446
RefSeq: XP_001706081.1
UniProt: A8BME4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYNANPKPSQ QGGARESRDS RDVRQQYIFL NIIGSGSFGK VHKVQSRTNG RIFACKEIDY AKMSEKEKKL LVHEVNTLKE LSHENIVSYI DRFVERENAK MFIVMEYCEN GDLAKYIKRH KTDRRYIAEE KIWSVFVQLL HALNYCHSIH SEDESGVHKV IHRDIKPGNV FLTQDGSIKL GDFGLCRTLG EDSVAQTNVG TPLYMAIEVL QKQSYTEKAD IWSLGCVIYE LCALQPPFVA SNIESLKTKV KQGARPAIPS HYSSDLSAAI DLMLNPNPSA RPSATDILQH SRIIEMNERI KAMPAYAAQA PPEIPKSAPP GGGHAQRERE LREWEERLNT KEQSLRAREE HVDRTLAALN AGTYKGY
NT Sequence
ATGTATAATG CCAATCCAAA GCCTTCACAG CAGGGTGGAG CCCGCGAAAG CAGAGATTCT AGGGATGTGC GACAGCAGTA TATCTTCCTC AACATCATCG GAAGCGGCTC CTTCGGGAAG GTCCACAAGG TCCAGTCCAG GACGAACGGG CGGATCTTCG CGTGCAAGGA AATCGACTAC GCCAAGATGA GCGAGAAGGA GAAGAAGCTC CTCGTCCACG AGGTCAACAC GCTCAAGGAG CTCAGCCACG AGAACATCGT CTCCTACATC GACCGCTTTG TCGAGCGCGA GAACGCGAAG ATGTTCATCG TCATGGAGTA CTGCGAGAAT GGAGACCTCG CAAAGTACAT CAAGCGGCAC AAGACAGACA GGCGCTACAT CGCTGAGGAG AAGATCTGGT CTGTCTTTGT CCAGCTCCTC CATGCCCTTA ACTATTGCCA TAGCATCCAT AGTGAAGACG AGTCAGGTGT CCACAAAGTT ATCCACCGGG ATATAAAGCC CGGTAACGTG TTCCTCACTC AAGATGGCTC CATCAAGCTG GGAGACTTTG GTCTCTGCCG TACCCTCGGA GAAGATTCTG TGGCCCAGAC AAACGTCGGC ACCCCCCTCT ACATGGCCAT AGAGGTCCTC CAGAAGCAGT CCTACACAGA GAAGGCAGAC ATATGGTCCC TCGGCTGCGT CATCTACGAG CTCTGTGCCC TGCAGCCCCC ATTCGTGGCA TCTAACATAG AGTCCCTCAA GACGAAGGTT AAGCAGGGTG CACGCCCTGC CATACCGTCT CACTACTCTA GCGATTTGTC AGCTGCAATC GACTTAATGC TCAACCCCAA TCCTTCAGCA CGACCATCTG CAACAGATAT CCTTCAGCAC AGTCGTATCA TTGAGATGAA TGAGAGAATA AAAGCTATGC CTGCATATGC TGCACAGGCG CCGCCTGAAA TACCAAAGAG TGCTCCACCA GGCGGTGGAC ATGCCCAACG TGAACGTGAA CTTCGTGAGT GGGAAGAACG CCTGAACACG AAGGAGCAGT CCCTACGAGC ACGTGAGGAG CACGTTGACC GCACACTAGC AGCTCTGAAC GCCGGAACAT ATAAAGGCTA T
Details for GilaA.01193.a.A1.GE25728
HARVESTED ON: 10/31/2008
SEQUENCED ON: 7/20/2010
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMYNANPKPS QQGGARESRD SRDVRQQYIF LNIIGSGSFG KVHKVQSRTN GRIFACKEID YAKMSEKEKK LLVHEVNTLK ELSHENIVSY IDRFVERENA KMFIVMEYCE NGDLAKYIKR HKTDRRYIAE EKIWSVFVQL LHALNYCHSI HSEDESGVHK VIHRDIKPGN VFLTQDGSIK LGDFGLCRTL GEDSVAQTNV GTPLYMAIEV LQKQSYTEKA DIWSLGCVIY ELCXLQPPFV ASNIESLKTK VKQGARPAIP SHYSSDLSAA IDLMLNPNPS ARPSATDILQ HSRIIEMNER IKAMPXYAAQ APPEIPKSAP PGGGHAQRER ELREWEERLN TKEQSLRARE XHVDRTLAAL NAGTYKG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtata atgccaatcc aaagccttca cagcagggtg gagcccgcga aagcagagat tctagggatg tgcgacagca gtatatcttc ctcaacatca tcggaagcgg ctccttcggg aaggtccaca aggtccagtc caggacgaac gggcggatct tcgcgtgcaa ggaaatcgac tacgccaaga tgagcgagaa ggagaagaag ctcctcgtcc acgaggtcaa cacgctcaag gagctcagcc acgagaacat cgtctcctac atcgaccgct ttgtcgagcg cgagaacgcg aagatgttca tcgtcatgga gtactgcgag aatggagacc tcgcaaagta catcaagcgg cacaagacag acaggcgcta catcgctgag gagaagatct ggtctgtctt tgtccagctc ctccatgccc ttaactattg ccatagcatc catagtgaag acgagtcagg tgtccacaaa gttatccacc gggatataaa gcccggtaac gtgttcctca ctcaagatgg ctccatcaag ctgggagact ttggtctctg ccgtaccctc ggagaagatt ctgtggccca gacaaacgtc ggcacccccc tctacatggc catagaggtc ctccagaagc agtcctacac agagaaggca gacatatggt ccctcggctg cgtcatctac gagctctgtg ncctgcagcc cccattcgtg gcatctaaca tagagtccct caagacgaag gttaagcagg gtgcacgccc tgccataccg tctcactact ctagcgattt gtcagctgca atcgacttaa tgctcaaccc caatccttca gcacgaccat ctgcaacaga tatccttcag cacagtcgta tcattgagat gaatgagaga ataaaagcta tgccnncata tgctgcacag gcgccgcctg aaataccaaa gagtgctcca ccaggcggtg gacatgccca acgtgaacgt gaacttcgtg agtgggaaga acgcctgaac acgaaggagc agtccctacg agcacgtgag gngcacgttg accgcacact agcagctctg aacgccggaa catataaagg ctat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMYNANPKPS QQGGARESRD SRDVRQQYIF LNIIGSGSFG KVHKVQSRTN GRIFACKEID YAKMSEKEKK LLVHEVNTLK ELSHENIVSY IDRFVERENA KMFIVMEYCE NGDLAKYIKR HKTDRRYIAE EKIWSVFVQL LHALNYCHSI HSEDESGVHK VIHRDIKPGN VFLTQDGSIK LGDFGLCRTL GEDSVAQTNV GTPLYMAIEV LQKQSYTEKA DIWSLGCVIY ELCALQPPFV ASNIESLKTK VKQGARPAIP SHYSSDLSAA IDLMLNPNPS ARPSATDILQ HSRIIEMNER IKAMPAYAAQ APPEIPKSAP PGGGHAQRER ELREWEERLN TKEQSLRARE EHVDRTLAAL NAGTYKGY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtataatgcc aatccaaagc cttcacagca gggtggagcc cgcgaaagca gagattctag ggatgtgcga cagcagtata tcttcctcaa catcatcgga agcggctcct tcgggaaggt ccacaaggtc cagtccagga cgaacgggcg gatcttcgcg tgcaaggaaa tcgactacgc caagatgagc gagaaggaga agaagctcct cgtccacgag gtcaacacgc tcaaggagct cagccacgag aacatcgtct cctacatcga ccgctttgtc gagcgcgaga acgcgaagat gttcatcgtc atggagtact gcgagaatgg agacctcgca aagtacatca agcggcacaa gacagacagg cgctacatcg ctgaggagaa gatctggtct gtctttgtcc agctcctcca tgcccttaac tattgccata gcatccatag tgaagacgag tcaggtgtcc acaaagttat ccaccgggat ataaagcccg gtaacgtgtt cctcactcaa gatggctcca tcaagctggg agactttggt ctctgccgta ccctcggaga agattctgtg gcccagacaa acgtcggcac ccccctctac atggccatag aggtcctcca gaagcagtcc tacacagaga aggcagacat atggtccctc ggctgcgtca tctacgagct ctgtgccctg cagcccccat tcgtggcatc taacatagag tccctcaaga cgaaggttaa gcagggtgca cgccctgcca taccgtctca ctactctagc gatttgtcag ctgcaatcga cttaatgctc aaccccaatc cttcagcacg accatctgca acagatatcc ttcagcacag tcgtatcatt gagatgaatg agagaataaa agctatgcct gcatatgctg cacaggcgcc gcctgaaata ccaaagagtg ctccaccagg cggtggacat gcccaacgtg aacgtgaact tcgtgagtgg gaagaacgcc tgaacacgaa ggagcagtcc ctacgagcac gtgaggagca cgttgaccgc acactagcag ctctgaacgc cggaacatat aaaggctata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a