GilaA.01158.b

Kinase, CMGC GSK

CENTER ID: GilaA.01158.b
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.01158.b.A1.GE30876 full length 1 370
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_9116
RefSeq: XP_001708858.1
UniProt: A8B7Q4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDDETAFPP EFELQLGKAL DIPGEKGEEG IQVVPEKVIG KGSFGVVYLV KSLKGNTPLA LKLVLQDRRY KNREFQIMRT LVHPCICRLV KGTFIPSISS PDDLILYLLM DYYPETIHSI YRQYSRLRQH VPLLIVRLIT YQLARSLAYL HYHNIAHRDC KPTNVLIDPL TYRCVLCDFG SAKQLVPQEN NVAYICSRFY RAPELVIGNQ NYTCAIDIWS FGCIMAEILL GQPLFPGDSS IDQLVEIVRV LGSPTAEEFL AMNPTLKSVK FKDVKRYPFS KIFRSKAHTD AVDLLTKVLV YDPKKRPTAM EILAHPFYDI LRDPDTLLPN GERLPAFLFE FTEEERKAAG PELMAVLTKN REQFNILSSH
NT Sequence
atgagcgatg acgagacagc gttcccccca gagtttgagc tccagctggg gaaggccctt gacattcccg gagaaaaggg ggaggagggg attcaggtgg tgcccgagaa ggtcataggg aagggaagct ttggggttgt ttacttagta aagtcattga agggaaacac tcctctcgcc cttaagctag tcttacaaga taggcggtat aagaacaggg aattccaaat tatgaggacc cttgttcatc cgtgcatttg cagattagtg aaaggcacct ttataccgtc catttcgtcg cccgatgatc taattcttta ccttctcatg gactattacc ccgaaactat tcattctatc taccgtcaat actcgcgact ccgccaacac gttccccttc tcatagttcg gctcattaca tatcagttgg cacgctctct cgcatacctc cactaccaca acatcgcaca cagagactgc aaaccaacga atgttctcat tgatcccttg acgtacaggt gtgtcctgtg cgacttcggc tccgccaagc agctggtgcc ccaagaaaac aacgtagcgt acatatgcag tcgcttctat cgggctcccg agctggttat tggcaaccag aactatacct gcgcgatcga catctggtcc ttcggctgca tcatggcaga aattctcctc ggccagcccc ttttccctgg tgactcttcc atagaccagc tagtagaaat agtacgcgtc cttggcagcc caacagccga agagttctta gctatgaacc ccacattgaa gagcgtgaag ttcaaggacg tcaagcgata tcccttctcc aagatcttta gaagcaaggc ccatacagac gcggttgatt tactaacgaa ggttctagtc tacgatccaa agaagcgccc cacggcaatg gaaatcctcg cgcatccttt ctacgacatc ttgcgcgatc cagacacgct gcttcccaat ggtgagcggc ttccagcatt cctcttcgag ttcacagagg aggaaaggaa ggcagcgggc cctgagctta tggccgttct aacaaagaac agagaacagt tcaacatctt gtcaagccac tga
Details for GilaA.01158.b.A1.GE30876
HARVESTED ON: 11/16/2010
SEQUENCED ON: 11/23/2010
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MSDDETAFPP EFELQLGKAL DIPGEKGEEG IQVVPEKVIG KGSFGVVYLV KSLKGNTPLA LKLVLQDRRY KNREFQIMRT LVHPCICRLV KGTFIPSISS PDDLILYLLM DYYPETIHSI YRQYSRLRQH VPLLIVRLIT YQLARSLAYL HYHNIAHRDC KPTNVLIDPL TYRCVLCDFG SAKQLVPQEN NVAYICSRFY RAPELVIGNQ NYTCAIDIWS FGCIMAEILL GQPLFPGDSS IDQLVEIVRV LGSPTAEEFL AMNPTLKSVK FKDVKRYPFS KIFRSKAHTD AVDLLTKVLV YDPKKRPTAM EILAHPFYDI LRDPDTLLPN GERLPAFLFE FTEEERKAAG PELMAVLTKN REQFNILSS
Validated NT Sequence
tggcttgaca agatgttgaa ctgttctctg ttctttgtta gaacggccat aagctcaggg cccgctgcct tcctttcctc ctctgtgaac tcgaagagga atgctggaag ccgctcacca ttgggaagca gcgtgtctgg atcgcgcaag atgtcgtaga aaggatgcgc gaggatttcc attgccgtgg ggcgcttctt tggatcgtag actagaacct tcgttagtaa atcaaccgcg tctgtatggg ccttgcttct aaagatcttg gagaagggat atcgcttgac gtccttgaac ttcacgctct tcaatgtggg gttcatagct aagaactctt cggctgttgg gctgccaagg acgcgtacta tttctactag ctggtctatg gaagagtcac cagggaaaag gggctggccg aggagaattt ctgccatgat gcagccgaag gaccagatgt cgatcgcgca ggtatagttc tggttgccaa taaccagctc gggagcccga tagaagcgac tgcatatgta cgctacgttg ttttcttggg gcaccagctg cttggcggag ccgaagtcgc acaggacaca cctgtacgtc aagggatcaa tgagaacatt cgttggtttg cagtctctgt gtgcgatgtt gtggtagtgg aggtatgcga gagagcgtgc caactgatat gtaatgagcc gaactatgag aaggggaacg tgttggcgga gtcgcgagta ttgacggtag atagaatgaa tagtttcggg gtaatagtcc atgagaaggt aaagaattag atcatcgggc gacgaaatgg acggtataaa ggtgcctttc actaatctgc aaatgcacgg atgaacaagg gtcctcataa tttggaattc cctgttctta taccgcctat cttgtaagac tagcttaagg gcgagaggag tgtttccctt caatgacttt actaagtaaa caaccccaaa gcttcccttc cctatgacct tctcgggcac cacctgaatc ccctcctccc ccttttctcc gggaatgtca agggccttcc ccagctggag ctcaaactct ggggggaacg ctgtctcgtc atcgctcatc gaaccaggac cctgggtctg agcttccagg gta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDDETAFP PEFELQLGKA LDIPGEKGEE GIQVVPEKVI GKGSFGVVYL VKSLKGNTPL ALKLVLQDRR YKNREFQIMR TLVHPCICRL VKGTFIPSIS SPDDLILYLL MDYYPETIHS IYRQYSRLRQ HVPLLIVRLI TYQLARSLAY LHYHNIAHRD CKPTNVLIDP LTYRCVLCDF GSAKQLVPQE NNVAYICSRF YRAPELVIGN QNYTCAIDIW SFGCIMAEIL LGQPLFPGDS SIDQLVEIVR VLGSPTAEEF LAMNPTLKSV KFKDVKRYPF SKIFRSKAHT DAVDLLTKVL VYDPKKRPTA MEILAHPFYD ILRDPDTLLP NGERLPAFLF EFTEEERKAA GPELMAVLTK NREQFNILSS H
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgatgac gagacagcgt tccccccaga gtttgagctc cagctgggga aggcccttga cattcccgga gaaaaggggg aggaggggat tcaggtggtg cccgagaagg tcatagggaa gggaagcttt ggggttgttt acttagtaaa gtcattgaag ggaaacactc ctctcgccct taagctagtc ttacaagata ggcggtataa gaacagggaa ttccaaatta tgaggaccct tgttcatccg tgcatttgca gattagtgaa aggcaccttt ataccgtcca tttcgtcgcc cgatgatcta attctttacc ttctcatgga ctattacccc gaaactattc attctatcta ccgtcaatac tcgcgactcc gccaacacgt tccccttctc atagttcggc tcattacata tcagttggca cgctctctcg catacctcca ctaccacaac atcgcacaca gagactgcaa accaacgaat gttctcattg atcccttgac gtacaggtgt gtcctgtgcg acttcggctc cgccaagcag ctggtgcccc aagaaaacaa cgtagcgtac atatgcagtc gcttctatcg ggctcccgag ctggttattg gcaaccagaa ctatacctgc gcgatcgaca tctggtcctt cggctgcatc atggcagaaa ttctcctcgg ccagcccctt ttccctggtg actcttccat agaccagcta gtagaaatag tacgcgtcct tggcagccca acagccgaag agttcttagc tatgaacccc acattgaaga gcgtgaagtt caaggacgtc aagcgatatc ccttctccaa gatctttaga agcaaggccc atacagacgc ggttgattta ctaacgaagg ttctagtcta cgatccaaag aagcgcccca cggcaatgga aatcctcgcg catcctttct acgacatctt gcgcgatcca gacacgctgc ttcccaatgg tgagcggctt ccagcattcc tcttcgagtt cacagaggag gaaaggaagg cagcgggccc tgagcttatg gccgttctaa caaagaacag agaacagttc aacatcttgt caagccacta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a