GilaA.01141.a
Ribokinase
| CENTER ID: | GilaA.01141.a |
| ORGANISM: | Giardia lamblia ATCC 50803 |
| ASSOCIATED DISEASE: | Giardiasis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| GilaA.01141.a.A1.GE25724 | Full length( GilaA.01141.a ) | 1 | 344 | ||
| GilaA.01141.a.AE1.GE43227 | Full length( GilaA.01141.a ) | 1 | 344 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| GilaA.01141.a.A1.PS00294 | Full length( GilaA.01141.a ) | 1 | 344 | ||
| GilaA.01141.a.AE1.PS38632 | Full length( GilaA.01141.a ) | 1 | 344 | ||
| GilaA.01141.a.A1.PW25772 | Full length( GilaA.01141.a ) | 1 | 344 |
Structures
6WHJ
DEPOSITED: 4/8/2020
DETERMINATION: XRay
CLONE: GilaA.01141.a.AE1.GE43227
PROTEIN: GilaA.01141.a.AE1.PS38632
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | GiardiaDB:GL50803_15297 |
| RefSeq: | XP_001709091.1 |
| UniProt: | A8B6Y7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAASTAKIV IIGSYNRDLV WYVKDFPIGG QTINGSFASS HGGKGSNQAI ACGKVLRDPS RAFFVGAVGK DTFGDEILAH YRELGIPNCI KQVSGAPTGN AGIYVAESGE NMIVISEGAN GMLKPSLVPD LMAVLVKATL VVMQCEISPD NTLLFVEVIK QAKAQNSSLR FVFNPAPYRA DYDFSKILSI TDIFCPNELE ALEISGTGRL EGICDDSMMK ALVEKMSSLS PSLKFVLFTL GSRGSRIVQT KSYESRTVGI YSHGRAIDTS GAGDCFIGSF CVRLMELAEE STRGPSALND IDTIAEAARF ASVAAGISVT RKGTSASVPR RQEVDDALSK FSGK
NT Sequence
ATGGCGGCGG CATCCACGGC CAAGATAGTG ATCATTGGTA GCTACAATCG GGACCTGGTA TGGTATGTCA AAGACTTTCC TATTGGAGGT CAGACGATCA ATGGGAGCTT TGCGTCTTCC CACGGAGGTA AAGGGTCCAA CCAGGCCATA GCCTGCGGAA AGGTTCTTCG TGATCCATCC AGGGCCTTCT TTGTGGGTGC GGTTGGCAAG GACACTTTTG GGGACGAGAT TCTGGCTCAT TATAGGGAGC TTGGCATCCC CAACTGCATC AAGCAGGTGT CCGGGGCACC CACCGGTAAT GCAGGGATCT ATGTTGCGGA GTCAGGAGAA AACATGATAG TCATCTCTGA GGGTGCTAAT GGGATGCTAA AGCCAAGCCT GGTGCCGGAT TTGATGGCTG TGTTGGTGAA AGCCACACTT GTAGTCATGC AGTGCGAAAT TTCTCCAGAC AATACACTCT TGTTTGTCGA GGTCATCAAG CAGGCTAAGG CACAGAATAG TAGTCTTCGA TTTGTGTTCA ACCCAGCACC GTATAGAGCT GACTATGACT TCAGCAAGAT CCTTTCCATC ACGGACATCT TCTGTCCTAA TGAGCTGGAG GCGCTTGAGA TATCTGGTAC AGGCAGACTT GAAGGGATTT GCGACGATTC TATGATGAAG GCTCTTGTGG AGAAGATGTC TAGTCTTTCG CCTTCTCTCA AGTTCGTTCT TTTCACGCTG GGTAGCAGGG GGTCTCGCAT AGTGCAGACA AAAAGCTATG AGTCTCGGAC TGTTGGGATA TACTCCCACG GGAGGGCGAT TGACACATCA GGAGCTGGAG ACTGCTTTAT TGGCAGCTTT TGTGTGCGTC TTATGGAACT GGCCGAGGAG TCTACTCGTG GACCATCTGC ACTTAATGAC ATCGACACTA TTGCAGAGGC TGCGCGCTTT GCAAGTGTTG CGGCAGGCAT CTCTGTAACC CGCAAGGGTA CCTCTGCGTC GGTTCCCAGG CGTCAAGAAG TGGATGATGC CCTGTCTAAA TTTTCTGGTA AA
Details for GilaA.01141.a.A1.GE25724
| HARVESTED ON: | 10/31/2008 |
| SEQUENCED ON: | 7/20/2010 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 50 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAAASTAKI VIIGSYNRDL VWYVKDFPIG GQTINGSFAS SHGGKGSNQA IACGKVLRDP SRAFFVGAVG KDTFGDEILA HYRELGIPNC IKQVSGAPTG NAGIYVAESG ENMIVISXGA NGMLKPSLVP DLMAVLVXAT LVVMQCEISP DNTLLFVXVI KXA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgg cggcatccac ggccaagata gtgatcattg gtagctacaa tcgggacctg gtatggtatg tcaaagactt tcctattgga ggtcagacga tcaatgggag ctttgcgtct tcccacggag gtaaagggtc caaccaggcc atagcctgcg gaaaggttct tcgtgatcca tccagggcct tctttgtggg tgcggttggc aaggacactt ttggggacga gattctggct cattataggg agcttggcat ccccaactgc atcaagcagg tgtccggggc acccaccggt aatgcaggga tctatgttgc ggagtcagga gaaaacatga tagtcatctc tganggtgct aatgggatgc taaagccaag cctggtgccg gatttgatgg ctgtgttggt gaangccaca cttgtagtca tgcagtgcga aatttctcca gacaatacac tcttgtttgt cnaggtcatc aagcangcta a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAASTAKI VIIGSYNRDL VWYVKDFPIG GQTINGSFAS SHGGKGSNQA IACGKVLRDP SRAFFVGAVG KDTFGDEILA HYRELGIPNC IKQVSGAPTG NAGIYVAESG ENMIVISEGA NGMLKPSLVP DLMAVLVKAT LVVMQCEISP DNTLLFVEVI KQAKAQNSSL RFVFNPAPYR ADYDFSKILS ITDIFCPNEL EALEISGTGR LEGICDDSMM KALVEKMSSL SPSLKFVLFT LGSRGSRIVQ TKSYESRTVG IYSHGRAIDT SGAGDCFIGS FCVRLMELAE ESTRGPSALN DIDTIAEAAR FASVAAGISV TRKGTSASVP RRQEVDDALS KFSGK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggcggca tccacggcca agatagtgat cattggtagc tacaatcggg acctggtatg gtatgtcaaa gactttccta ttggaggtca gacgatcaat gggagctttg cgtcttccca cggaggtaaa gggtccaacc aggccatagc ctgcggaaag gttcttcgtg atccatccag ggccttcttt gtgggtgcgg ttggcaagga cacttttggg gacgagattc tggctcatta tagggagctt ggcatcccca actgcatcaa gcaggtgtcc ggggcaccca ccggtaatgc agggatctat gttgcggagt caggagaaaa catgatagtc atctctgagg gtgctaatgg gatgctaaag ccaagcctgg tgccggattt gatggctgtg ttggtgaaag ccacacttgt agtcatgcag tgcgaaattt ctccagacaa tacactcttg tttgtcgagg tcatcaagca ggctaaggca cagaatagta gtcttcgatt tgtgttcaac ccagcaccgt atagagctga ctatgacttc agcaagatcc tttccatcac ggacatcttc tgtcctaatg agctggaggc gcttgagata tctggtacag gcagacttga agggatttgc gacgattcta tgatgaaggc tcttgtggag aagatgtcta gtctttcgcc ttctctcaag ttcgttcttt tcacgctggg tagcaggggg tctcgcatag tgcagacaaa aagctatgag tctcggactg ttgggatata ctcccacggg agggcgattg acacatcagg agctggagac tgctttattg gcagcttttg tgtgcgtctt atggaactgg ccgaggagtc tactcgtgga ccatctgcac ttaatgacat cgacactatt gcagaggctg cgcgctttgc aagtgttgcg gcaggcatct ctgtaacccg caagggtacc tctgcgtcgg ttcccaggcg tcaagaagtg gatgatgccc tgtctaaatt ttctggtaaa aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for GilaA.01141.a.AE1.GE43227
| HARVESTED ON: | 6/12/2019 |
| SEQUENCED ON: | 6/18/2019 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MMAAXSTXXI VIIGSYXRDL XWYVKDFPIG GXTINGSFAS SHGGKGSNXA IACGKVLXDP SXAFFVGAVG KDTFGDEILA HYRELGIPNC IKQVSGAPTG NAGIYVAESG ENMIVISEGA NGMLKPSLVP DLMAVLVKAT LVVMQCEISP DNTLLFVEVI KQAKAQNSSL RFVFNPAPYR ADYDFSKILS ITDIFCPNEL EALEISGTGR LEGICDDSMM KALVEKMSSL SPSLKFVLFT LGSRGSRIVQ TKSYESRTVG IYSHGRAIDT SGAGDCFIGS FCVRLMELAE ESTRGPSAXN DIDTIAEAAR FASVAAGISV TRKGTSASVP RXQEVDDAXX KFSGKAGHHH HHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag ctttaccaga aaatttanac ngggcatcat ccacttcttg acncctggga accgacgcag aggtaccctt gcgggttaca gagatgcctg ccgcaacact tgcaaagcgc gcagcctctg caatagtgtc gatgtcatta antgcagatg gtccacgagt agactcctcg gccagttcca taagacgcac acaaaagctg ccaataaagc agtctccagc tcctgatgtg tcaatcgccc tcccgtggga gtatatccca acagtccgag actcatagct ttttgtctgc actatgcgag accccctgct acccagcgtg aaaagaacga acttgagaga aggcgaaaga ctagacatct tctccacaag agccttcatc atagaatcgt cgcaaatccc ttcaagtctg cctgtaccag atatctcaag cgcctccagc tcattaggac agaagatgtc cgtgatggaa aggatcttgc tgaagtcata gtcagctcta tacggtgctg ggttgaacac aaatcgaaga ctactattct gtgccttagc ctgcttgatg acctcgacaa acaagagtgt attgtctgga gaaatttcgc actgcatgac tacaagtgtg gctttcacca acacagccat caaatccggc accaggcttg gctttagcat cccattagca ccctcagaga tgactatcat gttttctcct gactccgcaa catagatccc tgcattaccg gtgggtgccc cggacacctg cttgatgcag ttggggatgc caagctccct ataatgagcc agaatctcgt ccccaaaagt gtccttgcca accgcaccca caaagaaggc cntggatgga tcnngaagaa cctttccgca ggctatggcn tggttggacc ctttacctcc gtgggaagan gcaaagctcc cattgatcgt ntgacctcca ataggaaagt ctttgacata ccatancagg tcccgatngt agctaccaat gatcactatn ttgnccgtgg annccgccgc catcatggga gaga
Expected Protein Sequence
MAAASTAKIV IIGSYNRDLV WYVKDFPIGG QTINGSFASS HGGKGSNQAI ACGKVLRDPS RAFFVGAVGK DTFGDEILAH YRELGIPNCI KQVSGAPTGN AGIYVAESGE NMIVISEGAN GMLKPSLVPD LMAVLVKATL VVMQCEISPD NTLLFVEVIK QAKAQNSSLR FVFNPAPYRA DYDFSKILSI TDIFCPNELE ALEISGTGRL EGICDDSMMK ALVEKMSSLS PSLKFVLFTL GSRGSRIVQT KSYESRTVGI YSHGRAIDTS GAGDCFIGSF CVRLMELAEE STRGPSALND IDTIAEAARF ASVAAGISVT RKGTSASVPR RQEVDDALSK FSGKGHHHHH H
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat ggcggcggca tccacggcca agatagtgat cattggtagc tacaatcggg acctggtatg gtatgtcaaa gactttccta ttggaggtca gacgatcaat gggagctttg cgtcttccca cggaggtaaa gggtccaacc aggccatagc ctgcggaaag gttcttcgtg atccatccag ggccttcttt gtgggtgcgg ttggcaagga cacttttggg gacgagattc tggctcatta tagggagctt ggcatcccca actgcatcaa gcaggtgtcc ggggcaccca ccggtaatgc agggatctat gttgcggagt caggagaaaa catgatagtc atctctgagg gtgctaatgg gatgctaaag ccaagcctgg tgccggattt gatggctgtg ttggtgaaag ccacacttgt agtcatgcag tgcgaaattt ctccagacaa tacactcttg tttgtcgagg tcatcaagca ggctaaggca cagaatagta gtcttcgatt tgtgttcaac ccagcaccgt atagagctga ctatgacttc agcaagatcc tttccatcac ggacatcttc tgtcctaatg agctggaggc gcttgagata tctggtacag gcagacttga agggatttgc gacgattcta tgatgaaggc tcttgtggag aagatgtcta gtctttcgcc ttctctcaag ttcgttcttt tcacgctggg tagcaggggg tctcgcatag tgcagacaaa aagctatgag tctcggactg ttgggatata ctcccacggg agggcgattg acacatcagg agctggagac tgctttattg gcagcttttg tgtgcgtctt atggaactgg ccgaggagtc tactcgtgga ccatctgcac ttaatgacat cgacactatt gcagaggctg cgcgctttgc aagtgttgcg gcaggcatct ctgtaacccg caagggtacc tctgcgtcgg ttcccaggcg tcaagaagtg gatgatgccc tgtctaaatt ttctggtaaa gggcaccacc atcatcatca ttaacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat
Details for GilaA.01141.a.A1.PS00294
| PURIFICATION DATe: | 7/2/2009 |
| CONCENTRATION: | 7.78mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 50 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAAASTAKI VIIGSYNRDL VWYVKDFPIG GQTINGSFAS SHGGKGSNQA IACGKVLRDP SRAFFVGAVG KDTFGDEILA HYRELGIPNC IKQVSGAPTG NAGIYVAESG ENMIVISXGA NGMLKPSLVP DLMAVLVXAT LVVMQCEISP DNTLLFVXVI KXA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgg cggcatccac ggccaagata gtgatcattg gtagctacaa tcgggacctg gtatggtatg tcaaagactt tcctattgga ggtcagacga tcaatgggag ctttgcgtct tcccacggag gtaaagggtc caaccaggcc atagcctgcg gaaaggttct tcgtgatcca tccagggcct tctttgtggg tgcggttggc aaggacactt ttggggacga gattctggct cattataggg agcttggcat ccccaactgc atcaagcagg tgtccggggc acccaccggt aatgcaggga tctatgttgc ggagtcagga gaaaacatga tagtcatctc tganggtgct aatgggatgc taaagccaag cctggtgccg gatttgatgg ctgtgttggt gaangccaca cttgtagtca tgcagtgcga aatttctcca gacaatacac tcttgtttgt cnaggtcatc aagcangcta a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAASTAKI VIIGSYNRDL VWYVKDFPIG GQTINGSFAS SHGGKGSNQA IACGKVLRDP SRAFFVGAVG KDTFGDEILA HYRELGIPNC IKQVSGAPTG NAGIYVAESG ENMIVISEGA NGMLKPSLVP DLMAVLVKAT LVVMQCEISP DNTLLFVEVI KQAKAQNSSL RFVFNPAPYR ADYDFSKILS ITDIFCPNEL EALEISGTGR LEGICDDSMM KALVEKMSSL SPSLKFVLFT LGSRGSRIVQ TKSYESRTVG IYSHGRAIDT SGAGDCFIGS FCVRLMELAE ESTRGPSALN DIDTIAEAAR FASVAAGISV TRKGTSASVP RRQEVDDALS KFSGK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGGCGGCA TCCACGGCCA AGATAGTGAT CATTGGTAGC TACAATCGGG ACCTGGTATG GTATGTCAAA GACTTTCCTA TTGGAGGTCA GACGATCAAT GGGAGCTTTG CGTCTTCCCA CGGAGGTAAA GGGTCCAACC AGGCCATAGC CTGCGGAAAG GTTCTTCGTG ATCCATCCAG GGCCTTCTTT GTGGGTGCGG TTGGCAAGGA CACTTTTGGG GACGAGATTC TGGCTCATTA TAGGGAGCTT GGCATCCCCA ACTGCATCAA GCAGGTGTCC GGGGCACCCA CCGGTAATGC AGGGATCTAT GTTGCGGAGT CAGGAGAAAA CATGATAGTC ATCTCTGAGG GTGCTAATGG GATGCTAAAG CCAAGCCTGG TGCCGGATTT GATGGCTGTG TTGGTGAAAG CCACACTTGT AGTCATGCAG TGCGAAATTT CTCCAGACAA TACACTCTTG TTTGTCGAGG TCATCAAGCA GGCTAAGGCA CAGAATAGTA GTCTTCGATT TGTGTTCAAC CCAGCACCGT ATAGAGCTGA CTATGACTTC AGCAAGATCC TTTCCATCAC GGACATCTTC TGTCCTAATG AGCTGGAGGC GCTTGAGATA TCTGGTACAG GCAGACTTGA AGGGATTTGC GACGATTCTA TGATGAAGGC TCTTGTGGAG AAGATGTCTA GTCTTTCGCC TTCTCTCAAG TTCGTTCTTT TCACGCTGGG TAGCAGGGGG TCTCGCATAG TGCAGACAAA AAGCTATGAG TCTCGGACTG TTGGGATATA CTCCCACGGG AGGGCGATTG ACACATCAGG AGCTGGAGAC TGCTTTATTG GCAGCTTTTG TGTGCGTCTT ATGGAACTGG CCGAGGAGTC TACTCGTGGA CCATCTGCAC TTAATGACAT CGACACTATT GCAGAGGCTG CGCGCTTTGC AAGTGTTGCG GCAGGCATCT CTGTAACCCG CAAGGGTACC TCTGCGTCGG TTCCCAGGCG TCAAGAAGTG GATGATGCCC TGTCTAAATT TTCTGGTAAA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for GilaA.01141.a.AE1.PS38632
| PURIFICATION DATe: | 1/14/2020 |
| CONCENTRATION: | 34.66mg/ml |
| OBSERVED MW: | 37kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MMAAXSTXXI VIIGSYXRDL XWYVKDFPIG GXTINGSFAS SHGGKGSNXA IACGKVLXDP SXAFFVGAVG KDTFGDEILA HYRELGIPNC IKQVSGAPTG NAGIYVAESG ENMIVISEGA NGMLKPSLVP DLMAVLVKAT LVVMQCEISP DNTLLFVEVI KQAKAQNSSL RFVFNPAPYR ADYDFSKILS ITDIFCPNEL EALEISGTGR LEGICDDSMM KALVEKMSSL SPSLKFVLFT LGSRGSRIVQ TKSYESRTVG IYSHGRAIDT SGAGDCFIGS FCVRLMELAE ESTRGPSAXN DIDTIAEAAR FASVAAGISV TRKGTSASVP RXQEVDDAXX KFSGKAGHHH HHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag ctttaccaga aaatttanac ngggcatcat ccacttcttg acncctggga accgacgcag aggtaccctt gcgggttaca gagatgcctg ccgcaacact tgcaaagcgc gcagcctctg caatagtgtc gatgtcatta antgcagatg gtccacgagt agactcctcg gccagttcca taagacgcac acaaaagctg ccaataaagc agtctccagc tcctgatgtg tcaatcgccc tcccgtggga gtatatccca acagtccgag actcatagct ttttgtctgc actatgcgag accccctgct acccagcgtg aaaagaacga acttgagaga aggcgaaaga ctagacatct tctccacaag agccttcatc atagaatcgt cgcaaatccc ttcaagtctg cctgtaccag atatctcaag cgcctccagc tcattaggac agaagatgtc cgtgatggaa aggatcttgc tgaagtcata gtcagctcta tacggtgctg ggttgaacac aaatcgaaga ctactattct gtgccttagc ctgcttgatg acctcgacaa acaagagtgt attgtctgga gaaatttcgc actgcatgac tacaagtgtg gctttcacca acacagccat caaatccggc accaggcttg gctttagcat cccattagca ccctcagaga tgactatcat gttttctcct gactccgcaa catagatccc tgcattaccg gtgggtgccc cggacacctg cttgatgcag ttggggatgc caagctccct ataatgagcc agaatctcgt ccccaaaagt gtccttgcca accgcaccca caaagaaggc cntggatgga tcnngaagaa cctttccgca ggctatggcn tggttggacc ctttacctcc gtgggaagan gcaaagctcc cattgatcgt ntgacctcca ataggaaagt ctttgacata ccatancagg tcccgatngt agctaccaat gatcactatn ttgnccgtgg annccgccgc catcatggga gaga
Expressed Protein Sequence
MAAASTAKIV IIGSYNRDLV WYVKDFPIGG QTINGSFASS HGGKGSNQAI ACGKVLRDPS RAFFVGAVGK DTFGDEILAH YRELGIPNCI KQVSGAPTGN AGIYVAESGE NMIVISEGAN GMLKPSLVPD LMAVLVKATL VVMQCEISPD NTLLFVEVIK QAKAQNSSLR FVFNPAPYRA DYDFSKILSI TDIFCPNELE ALEISGTGRL EGICDDSMMK ALVEKMSSLS PSLKFVLFTL GSRGSRIVQT KSYESRTVGI YSHGRAIDTS GAGDCFIGSF CVRLMELAEE STRGPSALND IDTIAEAARF ASVAAGISVT RKGTSASVPR RQEVDDALSK FSGKGHHHHH H
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCAT GGCGGCGGCA TCCACGGCCA AGATAGTGAT CATTGGTAGC TACAATCGGG ACCTGGTATG GTATGTCAAA GACTTTCCTA TTGGAGGTCA GACGATCAAT GGGAGCTTTG CGTCTTCCCA CGGAGGTAAA GGGTCCAACC AGGCCATAGC CTGCGGAAAG GTTCTTCGTG ATCCATCCAG GGCCTTCTTT GTGGGTGCGG TTGGCAAGGA CACTTTTGGG GACGAGATTC TGGCTCATTA TAGGGAGCTT GGCATCCCCA ACTGCATCAA GCAGGTGTCC GGGGCACCCA CCGGTAATGC AGGGATCTAT GTTGCGGAGT CAGGAGAAAA CATGATAGTC ATCTCTGAGG GTGCTAATGG GATGCTAAAG CCAAGCCTGG TGCCGGATTT GATGGCTGTG TTGGTGAAAG CCACACTTGT AGTCATGCAG TGCGAAATTT CTCCAGACAA TACACTCTTG TTTGTCGAGG TCATCAAGCA GGCTAAGGCA CAGAATAGTA GTCTTCGATT TGTGTTCAAC CCAGCACCGT ATAGAGCTGA CTATGACTTC AGCAAGATCC TTTCCATCAC GGACATCTTC TGTCCTAATG AGCTGGAGGC GCTTGAGATA TCTGGTACAG GCAGACTTGA AGGGATTTGC GACGATTCTA TGATGAAGGC TCTTGTGGAG AAGATGTCTA GTCTTTCGCC TTCTCTCAAG TTCGTTCTTT TCACGCTGGG TAGCAGGGGG TCTCGCATAG TGCAGACAAA AAGCTATGAG TCTCGGACTG TTGGGATATA CTCCCACGGG AGGGCGATTG ACACATCAGG AGCTGGAGAC TGCTTTATTG GCAGCTTTTG TGTGCGTCTT ATGGAACTGG CCGAGGAGTC TACTCGTGGA CCATCTGCAC TTAATGACAT CGACACTATT GCAGAGGCTG CGCGCTTTGC AAGTGTTGCG GCAGGCATCT CTGTAACCCG CAAGGGTACC TCTGCGTCGG TTCCCAGGCG TCAAGAAGTG GATGATGCCC TGTCTAAATT TTCTGGTAAA GGGCACCACC ATCATCATCA TTAACGGATC CGAATTCGAG CTCCGTCGAC AAGCTTGCGG CCGCACTCGA GCACCACCAC CACCACCACT GAGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGAT
Details for GilaA.01141.a.A1.PW25772
| PURIFICATION DATe: | 1/28/2009 |
| CONCENTRATION: | 33.61mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 29 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 50 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAAASTAKI VIIGSYNRDL VWYVKDFPIG GQTINGSFAS SHGGKGSNQA IACGKVLRDP SRAFFVGAVG KDTFGDEILA HYRELGIPNC IKQVSGAPTG NAGIYVAESG ENMIVISXGA NGMLKPSLVP DLMAVLVXAT LVVMQCEISP DNTLLFVXVI KXA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgg cggcatccac ggccaagata gtgatcattg gtagctacaa tcgggacctg gtatggtatg tcaaagactt tcctattgga ggtcagacga tcaatgggag ctttgcgtct tcccacggag gtaaagggtc caaccaggcc atagcctgcg gaaaggttct tcgtgatcca tccagggcct tctttgtggg tgcggttggc aaggacactt ttggggacga gattctggct cattataggg agcttggcat ccccaactgc atcaagcagg tgtccggggc acccaccggt aatgcaggga tctatgttgc ggagtcagga gaaaacatga tagtcatctc tganggtgct aatgggatgc taaagccaag cctggtgccg gatttgatgg ctgtgttggt gaangccaca cttgtagtca tgcagtgcga aatttctcca gacaatacac tcttgtttgt cnaggtcatc aagcangcta a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAASTAKI VIIGSYNRDL VWYVKDFPIG GQTINGSFAS SHGGKGSNQA IACGKVLRDP SRAFFVGAVG KDTFGDEILA HYRELGIPNC IKQVSGAPTG NAGIYVAESG ENMIVISEGA NGMLKPSLVP DLMAVLVKAT LVVMQCEISP DNTLLFVEVI KQAKAQNSSL RFVFNPAPYR ADYDFSKILS ITDIFCPNEL EALEISGTGR LEGICDDSMM KALVEKMSSL SPSLKFVLFT LGSRGSRIVQ TKSYESRTVG IYSHGRAIDT SGAGDCFIGS FCVRLMELAE ESTRGPSALN DIDTIAEAAR FASVAAGISV TRKGTSASVP RRQEVDDALS KFSGK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGGCGGCA TCCACGGCCA AGATAGTGAT CATTGGTAGC TACAATCGGG ACCTGGTATG GTATGTCAAA GACTTTCCTA TTGGAGGTCA GACGATCAAT GGGAGCTTTG CGTCTTCCCA CGGAGGTAAA GGGTCCAACC AGGCCATAGC CTGCGGAAAG GTTCTTCGTG ATCCATCCAG GGCCTTCTTT GTGGGTGCGG TTGGCAAGGA CACTTTTGGG GACGAGATTC TGGCTCATTA TAGGGAGCTT GGCATCCCCA ACTGCATCAA GCAGGTGTCC GGGGCACCCA CCGGTAATGC AGGGATCTAT GTTGCGGAGT CAGGAGAAAA CATGATAGTC ATCTCTGAGG GTGCTAATGG GATGCTAAAG CCAAGCCTGG TGCCGGATTT GATGGCTGTG TTGGTGAAAG CCACACTTGT AGTCATGCAG TGCGAAATTT CTCCAGACAA TACACTCTTG TTTGTCGAGG TCATCAAGCA GGCTAAGGCA CAGAATAGTA GTCTTCGATT TGTGTTCAAC CCAGCACCGT ATAGAGCTGA CTATGACTTC AGCAAGATCC TTTCCATCAC GGACATCTTC TGTCCTAATG AGCTGGAGGC GCTTGAGATA TCTGGTACAG GCAGACTTGA AGGGATTTGC GACGATTCTA TGATGAAGGC TCTTGTGGAG AAGATGTCTA GTCTTTCGCC TTCTCTCAAG TTCGTTCTTT TCACGCTGGG TAGCAGGGGG TCTCGCATAG TGCAGACAAA AAGCTATGAG TCTCGGACTG TTGGGATATA CTCCCACGGG AGGGCGATTG ACACATCAGG AGCTGGAGAC TGCTTTATTG GCAGCTTTTG TGTGCGTCTT ATGGAACTGG CCGAGGAGTC TACTCGTGGA CCATCTGCAC TTAATGACAT CGACACTATT GCAGAGGCTG CGCGCTTTGC AAGTGTTGCG GCAGGCATCT CTGTAACCCG CAAGGGTACC TCTGCGTCGG TTCCCAGGCG TCAAGAAGTG GATGATGCCC TGTCTAAATT TTCTGGTAAA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA