GilaA.01134.a

RabF

CENTER ID: GilaA.01134.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.01134.a.A1.GE25723 Full length( GilaA.01134.a ) 1 278
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_8497
RefSeq: XP_001705767.1
UniProt: Q8T694

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARFAELDES SDEQAPTVRT FKVTVLGDGS VGKSSICSRF AKDFFATSYK QTIGVDFFSQ TVCLDDGNKV TFNLYDIGGQ SIGSKMLSAY ISGTNALIFV YDLTNAESLE NVVEWYAMAR RSVKSMKDVV VYLVGNKSDL ATLRVVRSFR SNEISAAINA SAHLLLSALT GDRVGLLFIS LAGRLCGVDV TSMILDGKMG HTVEVSKDTE ETVSCINKDR FLGNADTVDG ASAVARAKDL ESRMAKKIQE LEGQATGIPH QTLVGVERAK RSGRCAVM
NT Sequence
ATGGCCCGGT TCGCGGAGCT GGATGAATCA TCCGATGAGC AGGCACCCAC GGTTCGCACA TTCAAAGTGA CGGTGCTTGG AGATGGGAGT GTTGGCAAGT CTTCCATTTG TAGCAGATTT GCAAAAGATT TCTTCGCTAC ATCGTACAAG CAGACCATCG GTGTGGACTT CTTTTCACAA ACTGTGTGCC TCGACGACGG CAACAAGGTT ACCTTTAACC TGTATGACAT TGGAGGGCAA TCAATTGGCT CCAAGATGCT TTCTGCATAC ATTTCTGGCA CAAACGCACT CATTTTTGTC TATGACCTTA CTAACGCCGA GTCTCTGGAG AACGTTGTGG AGTGGTATGC CATGGCGAGA AGATCGGTTA AGTCGATGAA AGATGTCGTC GTCTATCTGG TAGGCAACAA GTCTGATCTT GCCACCCTCC GGGTCGTCCG GTCCTTTCGA AGCAATGAGA TTTCTGCTGC CATTAATGCT TCCGCACATT TACTATTGTC GGCCTTGACT GGCGATCGCG TAGGCTTGCT CTTCATCAGC CTAGCAGGAC GTTTGTGCGG AGTGGATGTA ACCAGTATGA TTCTCGATGG AAAGATGGGT CACACAGTGG AGGTAAGCAA GGATACCGAA GAGACCGTCA GTTGCATCAA CAAGGATCGA TTTCTTGGTA ATGCAGACAC AGTTGACGGT GCTAGTGCTG TCGCCCGTGC AAAGGATCTG GAGAGTAGAA TGGCGAAGAA GATACAGGAA CTTGAGGGAC AGGCGACTGG AATCCCTCAT CAGACCCTGG TTGGTGTAGA GAGAGCAAAG CGATCCGGTC GGTGCGCTGT GATG
Details for GilaA.01134.a.A1.GE25723
HARVESTED ON: 10/31/2008
SEQUENCED ON: 7/20/2010
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMARFAELDE SSDEQAPTVR TFKVTVLGDG SVGKSSICSR FAKDFFATSY RQTIGVDFFS QTVCLDDGNK VTFNLYDIGG QSIGSKMLSA YISGTNALIF VYDLTNAESL ENVVEWYAMA RRSVKSMKDV VVYLVGNKSD LATLRVVRSF RSNEISAAIN ASAHLLLSAL TGDRVGLLFI SLAGRLCGVD VTSMILDGKM GHTVEVSKDT EETVSCINKD RFLGNADTVD GASAVARAKD LESRMAKKIQ ELEGQATGIP HQTLVGVERA KRSGRCAVM
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggccc ggttcgcgga gctggatgaa tcatccgatg agcaggcacc cacggttcgc acattcaaag tgacggtgct tggagatggg agtgttggca agtcttccat ttgtagcaga tttgcaaaag atttcttcgc tacatcgtac aggcagacca tcggtgtgga cttcttttca caaactgtgt gcctcgacga cggcaacaag gttaccttta acctgtatga cattggaggg caatcaattg gctccaagat gctttctgca tacatttctg gcacaaacgc actcattttt gtctatgacc ttactaacgc cgagtctctg gagaacgttg tggagtggta tgccatggcg agaagatcgg ttaagtcgat gaaagatgtc gtcgtctatc tggtaggcaa caagtctgat cttgccaccc tccgggtcgt ccggtccttt cgaagcaatg agatttctgc tgccattaat gcttccgcac atttactatt gtcggccttg actggcgatc gcgtaggctt gctcttcatc agcctagcag gacgtttgtg cggagtggat gtaaccagta tgattctcga tggaaagatg ggtcacacag tggaggtaag caaggatacc gaagagaccg tcagttgcat caacaaggat cgatttcttg gtaatgcaga cacagttgac ggtgctagtg ctgtcgcccg tgcaaaggat ctggagagta gaatggcgaa gaagatacag gaacttgagg gacaggcgac tggaatccct catcagaccc tggttggtgt agagagagca aagcgatccg gtcggtgcgc tgtgatgtaa taa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARFAELDE SSDEQAPTVR TFKVTVLGDG SVGKSSICSR FAKDFFATSY KQTIGVDFFS QTVCLDDGNK VTFNLYDIGG QSIGSKMLSA YISGTNALIF VYDLTNAESL ENVVEWYAMA RRSVKSMKDV VVYLVGNKSD LATLRVVRSF RSNEISAAIN ASAHLLLSAL TGDRVGLLFI SLAGRLCGVD VTSMILDGKM GHTVEVSKDT EETVSCINKD RFLGNADTVD GASAVARAKD LESRMAKKIQ ELEGQATGIP HQTLVGVERA KRSGRCAVM
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcccggttc gcggagctgg atgaatcatc cgatgagcag gcacccacgg ttcgcacatt caaagtgacg gtgcttggag atgggagtgt tggcaagtct tccatttgta gcagatttgc aaaagatttc ttcgctacat cgtacaagca gaccatcggt gtggacttct tttcacaaac tgtgtgcctc gacgacggca acaaggttac ctttaacctg tatgacattg gagggcaatc aattggctcc aagatgcttt ctgcatacat ttctggcaca aacgcactca tttttgtcta tgaccttact aacgccgagt ctctggagaa cgttgtggag tggtatgcca tggcgagaag atcggttaag tcgatgaaag atgtcgtcgt ctatctggta ggcaacaagt ctgatcttgc caccctccgg gtcgtccggt cctttcgaag caatgagatt tctgctgcca ttaatgcttc cgcacattta ctattgtcgg ccttgactgg cgatcgcgta ggcttgctct tcatcagcct agcaggacgt ttgtgcggag tggatgtaac cagtatgatt ctcgatggaa agatgggtca cacagtggag gtaagcaagg ataccgaaga gaccgtcagt tgcatcaaca aggatcgatt tcttggtaat gcagacacag ttgacggtgc tagtgctgtc gcccgtgcaa aggatctgga gagtagaatg gcgaagaaga tacaggaact tgagggacag gcgactggaa tccctcatca gaccctggtt ggtgtagaga gagcaaagcg atccggtcgg tgcgctgtga tgaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa