GilaA.01102.a

Endonuclease/Exonuclease/phosphatase

CENTER ID: GilaA.01102.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.01102.a.A1.GE25722 Full length( GilaA.01102.a ) 1 322
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.01102.a.A1.PW26149 Full length( GilaA.01102.a ) 1 322

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_9834
RefSeq: XP_001707172.1
UniProt: A8BGE2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPPAPVKMKK TSAAKERPPP PPYSTFQIGS DEKTEPVPFT ILAINVNGIR SAFSKGLKDF IHKKNPDIVC FSETKLGSSA FAAFMEKEAT RDPDTGVYTV MKGYKHVFCC STAKQGYAST AMFAKESVPV LDLCMQMGDP EFDSEGRFLH VSLPSFELIH VYAPNSGRGS SGKAFTSKNR PANLSTRIKY EKLISKYIGN LVAAGKDVVY CGDLNVAHNE IDLYNPKSNH FSPGFTDEER NAFSKLLDGH GLIDAFRAMH PHRINYSWFS NFGRAREQKH GWRIDYFVVT SKIFKRVTTV DILDDYNSYS DHVPIIMTLT GI
NT Sequence
ATGCCTCCTG CACCCGTTAA GATGAAGAAG ACCTCAGCTG CAAAGGAACG GCCTCCTCCC CCGCCGTACT CCACATTTCA GATAGGCTCA GATGAAAAGA CTGAACCAGT ACCGTTCACC ATTTTAGCAA TTAATGTTAA TGGCATAAGG TCGGCCTTCT CCAAGGGCCT CAAGGACTTT ATACACAAAA AGAACCCCGA TATAGTGTGC TTTTCAGAGA CAAAGCTGGG TTCGTCCGCC TTTGCCGCAT TCATGGAGAA GGAGGCCACC AGGGATCCAG ACACGGGGGT GTACACCGTG ATGAAGGGGT ATAAGCACGT TTTCTGCTGC TCTACCGCCA AGCAAGGGTA TGCATCCACT GCTATGTTCG CCAAAGAGTC TGTTCCTGTC TTAGACCTGT GTATGCAGAT GGGAGACCCG GAGTTCGATT CTGAGGGTCG CTTTTTGCAC GTTAGCCTTC CATCATTCGA GCTCATCCAT GTGTACGCCC CTAACTCCGG TCGCGGCTCA AGTGGGAAAG CGTTTACTTC CAAGAACAGG CCCGCCAATC TTTCCACACG TATAAAGTAT GAGAAGCTGA TATCCAAGTA CATAGGAAAC CTAGTTGCTG CAGGAAAGGA TGTTGTCTAC TGCGGAGACC TAAACGTTGC ACACAATGAA ATAGATCTCT ATAACCCGAA GAGCAACCAC TTCAGTCCCG GGTTTACCGA TGAAGAGCGT AACGCGTTCA GCAAGCTATT GGATGGACAC GGTCTGATAG ATGCATTTAG AGCTATGCAT CCTCACCGGA TTAACTATAG CTGGTTTAGC AACTTTGGAC GTGCACGAGA ACAGAAGCAT GGGTGGCGGA TAGATTACTT TGTGGTTACA AGTAAGATAT TTAAACGTGT AACGACGGTG GACATCTTAG ACGATTATAA TAGCTATAGT GACCATGTTC CCATTATAAT GACGCTCACG GGGATC
Details for GilaA.01102.a.A1.GE25722
HARVESTED ON: 10/31/2008
SEQUENCED ON: 7/20/2010
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPPAPVKMK KTSAAKERPP PPPYSTFQIG SDEKTEPVPF TILAINVNGI RSAFSKGLKD FIHKKNPDIV CFSETKLGSS AFAAFMEKEA TRDPDTGVYT VMKGYKHVFC CSTAKQGYAS TAMFAKESVP VLDLCMQMGD PEFDSEGRFL HVSLPSFELI HVYAPNSGRG SSGKAFTSKN RPANLSTRIK YEKLISKYIG NLVAAGKDVV YCGDLNVAHN EIDLYNPKSN HFSPGFTDEE RNAFSKLLDG HGLIDAFRAM HPHRINYSWF SNFGRAREQK HGWRIDYFVV TSKIFKRVTT VDILDDYNSY SDHVPIIMTL TGI
Validated NT Sequence
ttattagatc cccgtgagcg tcattataat gggaacatgg tcactatagc tattataatc gtctaagatg tccaccgtcg ttacacgttt aaatatctta cttgtaacca caaagtaatc tatccgccac ccatgcttct gttctcgtgc acgtccaaag ttgctaaacc agctatagtt aatccggtga ggatgcatag ctctaaatgc atctatcaga ccgtgtccat ccaatagctt gctgaacgcg ttacgctctt catcggtaaa cccgggactg aagtggttgc tcttcgggtt atagagatct atttcattgt gtgcaacgtt taggtctccg cagtagacaa catcctttcc tgcagcaact aggtttccta tgtacttgga tatcagcttc tcatacttta tacgtgtgga aagattggcg ggcctgttct tggaagtaaa cgctttccca cttgagccgc gaccggagtt aggggcgtac acatggatga gctcgaatga tggaaggcta acgtgcaaaa agcgaccctc agaatcgaac tccgggtctc ccatctgcat acacaggtct aagacaggaa cagactcttt ggcgaacata gcagtggatg catacccttg cttggcggta gagcagcaga aaacgtgctt ataccccttc atcacggtgt acacccccgt gtctggatcc ctggtggcct ccttctccat gaatgcggca aaggcggacg aacccagctt tgtctctgaa aagcacacta tatcggggtt ctttttgtgt ataaagtcct tgaggccctt ggagaaggcc gaccttatgc cattaacatt aattgctaaa atggtgaacg gtactggttc agtcttttca tctgagccta tctgaaatgt ggagtacggc gggggaggag gccgttcctt tgcagctgag gtcttcttca tcttaacggg tgcaggaggc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPPAPVKMK KTSAAKERPP PPPYSTFQIG SDEKTEPVPF TILAINVNGI RSAFSKGLKD FIHKKNPDIV CFSETKLGSS AFAAFMEKEA TRDPDTGVYT VMKGYKHVFC CSTAKQGYAS TAMFAKESVP VLDLCMQMGD PEFDSEGRFL HVSLPSFELI HVYAPNSGRG SSGKAFTSKN RPANLSTRIK YEKLISKYIG NLVAAGKDVV YCGDLNVAHN EIDLYNPKSN HFSPGFTDEE RNAFSKLLDG HGLIDAFRAM HPHRINYSWF SNFGRAREQK HGWRIDYFVV TSKIFKRVTT VDILDDYNSY SDHVPIIMTL TGI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcctcctgca cccgttaaga tgaagaagac ctcagctgca aaggaacggc ctcctccccc gccgtactcc acatttcaga taggctcaga tgaaaagact gaaccagtac cgttcaccat tttagcaatt aatgttaatg gcataaggtc ggccttctcc aagggcctca aggactttat acacaaaaag aaccccgata tagtgtgctt ttcagagaca aagctgggtt cgtccgcctt tgccgcattc atggagaagg aggccaccag ggatccagac acgggggtgt acaccgtgat gaaggggtat aagcacgttt tctgctgctc taccgccaag caagggtatg catccactgc tatgttcgcc aaagagtctg ttcctgtctt agacctgtgt atgcagatgg gagacccgga gttcgattct gagggtcgct ttttgcacgt tagccttcca tcattcgagc tcatccatgt gtacgcccct aactccggtc gcggctcaag tgggaaagcg tttacttcca agaacaggcc cgccaatctt tccacacgta taaagtatga gaagctgata tccaagtaca taggaaacct agttgctgca ggaaaggatg ttgtctactg cggagaccta aacgttgcac acaatgaaat agatctctat aacccgaaga gcaaccactt cagtcccggg tttaccgatg aagagcgtaa cgcgttcagc aagctattgg atggacacgg tctgatagat gcatttagag ctatgcatcc tcaccggatt aactatagct ggtttagcaa ctttggacgt gcacgagaac agaagcatgg gtggcggata gattactttg tggttacaag taagatattt aaacgtgtaa cgacggtgga catcttagac gattataata gctatagtga ccatgttccc attataatga cgctcacggg gatcaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for GilaA.01102.a.A1.PW26149
PURIFICATION DATe: 12/16/2008
CONCENTRATION: 27.84mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 25
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPPAPVKMK KTSAAKERPP PPPYSTFQIG SDEKTEPVPF TILAINVNGI RSAFSKGLKD FIHKKNPDIV CFSETKLGSS AFAAFMEKEA TRDPDTGVYT VMKGYKHVFC CSTAKQGYAS TAMFAKESVP VLDLCMQMGD PEFDSEGRFL HVSLPSFELI HVYAPNSGRG SSGKAFTSKN RPANLSTRIK YEKLISKYIG NLVAAGKDVV YCGDLNVAHN EIDLYNPKSN HFSPGFTDEE RNAFSKLLDG HGLIDAFRAM HPHRINYSWF SNFGRAREQK HGWRIDYFVV TSKIFKRVTT VDILDDYNSY SDHVPIIMTL TGI
Validated NT Sequence
ttattagatc cccgtgagcg tcattataat gggaacatgg tcactatagc tattataatc gtctaagatg tccaccgtcg ttacacgttt aaatatctta cttgtaacca caaagtaatc tatccgccac ccatgcttct gttctcgtgc acgtccaaag ttgctaaacc agctatagtt aatccggtga ggatgcatag ctctaaatgc atctatcaga ccgtgtccat ccaatagctt gctgaacgcg ttacgctctt catcggtaaa cccgggactg aagtggttgc tcttcgggtt atagagatct atttcattgt gtgcaacgtt taggtctccg cagtagacaa catcctttcc tgcagcaact aggtttccta tgtacttgga tatcagcttc tcatacttta tacgtgtgga aagattggcg ggcctgttct tggaagtaaa cgctttccca cttgagccgc gaccggagtt aggggcgtac acatggatga gctcgaatga tggaaggcta acgtgcaaaa agcgaccctc agaatcgaac tccgggtctc ccatctgcat acacaggtct aagacaggaa cagactcttt ggcgaacata gcagtggatg catacccttg cttggcggta gagcagcaga aaacgtgctt ataccccttc atcacggtgt acacccccgt gtctggatcc ctggtggcct ccttctccat gaatgcggca aaggcggacg aacccagctt tgtctctgaa aagcacacta tatcggggtt ctttttgtgt ataaagtcct tgaggccctt ggagaaggcc gaccttatgc cattaacatt aattgctaaa atggtgaacg gtactggttc agtcttttca tctgagccta tctgaaatgt ggagtacggc gggggaggag gccgttcctt tgcagctgag gtcttcttca tcttaacggg tgcaggaggc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPPAPVKMK KTSAAKERPP PPPYSTFQIG SDEKTEPVPF TILAINVNGI RSAFSKGLKD FIHKKNPDIV CFSETKLGSS AFAAFMEKEA TRDPDTGVYT VMKGYKHVFC CSTAKQGYAS TAMFAKESVP VLDLCMQMGD PEFDSEGRFL HVSLPSFELI HVYAPNSGRG SSGKAFTSKN RPANLSTRIK YEKLISKYIG NLVAAGKDVV YCGDLNVAHN EIDLYNPKSN HFSPGFTDEE RNAFSKLLDG HGLIDAFRAM HPHRINYSWF SNFGRAREQK HGWRIDYFVV TSKIFKRVTT VDILDDYNSY SDHVPIIMTL TGI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCTCCTGCA CCCGTTAAGA TGAAGAAGAC CTCAGCTGCA AAGGAACGGC CTCCTCCCCC GCCGTACTCC ACATTTCAGA TAGGCTCAGA TGAAAAGACT GAACCAGTAC CGTTCACCAT TTTAGCAATT AATGTTAATG GCATAAGGTC GGCCTTCTCC AAGGGCCTCA AGGACTTTAT ACACAAAAAG AACCCCGATA TAGTGTGCTT TTCAGAGACA AAGCTGGGTT CGTCCGCCTT TGCCGCATTC ATGGAGAAGG AGGCCACCAG GGATCCAGAC ACGGGGGTGT ACACCGTGAT GAAGGGGTAT AAGCACGTTT TCTGCTGCTC TACCGCCAAG CAAGGGTATG CATCCACTGC TATGTTCGCC AAAGAGTCTG TTCCTGTCTT AGACCTGTGT ATGCAGATGG GAGACCCGGA GTTCGATTCT GAGGGTCGCT TTTTGCACGT TAGCCTTCCA TCATTCGAGC TCATCCATGT GTACGCCCCT AACTCCGGTC GCGGCTCAAG TGGGAAAGCG TTTACTTCCA AGAACAGGCC CGCCAATCTT TCCACACGTA TAAAGTATGA GAAGCTGATA TCCAAGTACA TAGGAAACCT AGTTGCTGCA GGAAAGGATG TTGTCTACTG CGGAGACCTA AACGTTGCAC ACAATGAAAT AGATCTCTAT AACCCGAAGA GCAACCACTT CAGTCCCGGG TTTACCGATG AAGAGCGTAA CGCGTTCAGC AAGCTATTGG ATGGACACGG TCTGATAGAT GCATTTAGAG CTATGCATCC TCACCGGATT AACTATAGCT GGTTTAGCAA CTTTGGACGT GCACGAGAAC AGAAGCATGG GTGGCGGATA GATTACTTTG TGGTTACAAG TAAGATATTT AAACGTGTAA CGACGGTGGA CATCTTAGAC GATTATAATA GCTATAGTGA CCATGTTCCC ATTATAATGA CGCTCACGGG GATCAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA