SSGCID: Target Page

GilaA.01085.a

Ribosomal protein SA

CENTER ID:	GilaA.01085.a
ORGANISM:	Giardia lamblia ATCC 50803
ASSOCIATED DISEASE:	Giardiasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
GilaA.01085.a.A1.GE25720	Full length( GilaA.01085.a )		1	245

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	GiardiaDB:GL50803_7766
RefSeq:	XP_001707613.1
UniProt:	A8BE86

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTEKTSQAS KEYQLKEADV KKMLVATTHL GVRNIDRRMQ FYIFDRQKDG TFVFNLQKVW AKIVFAARIL VTIDDPAEIA VVANRPDAQR AILKFCKYTH ATAFPGRFIP GNFTNRMNPN YCEPRLLLVN DPVVDRQAIL EASYVNIPTI SLCNSDANLK FIDVAIPCNN KTPMSIGLIY WLLAREVLRL KGSISRTEEW DVKPDLFVAL PEEIPDEEES EDFYDDDEEE DEEFSAGNGN LFDEY

NT Sequence

ATGTCGACAG AGAAAACGTC CCAGGCGTCC AAGGAGTACC AGCTCAAGGA GGCTGATGTG AAGAAGATGC TCGTTGCAAC GACTCACCTT GGCGTCCGCA ACATCGATAG GAGGATGCAG TTCTACATCT TCGACAGGCA GAAGGATGGG ACCTTTGTCT TCAACCTGCA GAAAGTCTGG GCCAAGATCG TCTTCGCAGC ACGCATCCTC GTCACCATCG ATGACCCCGC CGAGATCGCC GTGGTCGCTA ACAGACCCGA TGCTCAGCGT GCTATCCTCA AGTTCTGCAA GTACACGCAC GCGACTGCCT TCCCCGGCCG CTTTATCCCT GGCAACTTCA CCAATCGTAT GAACCCGAAC TATTGTGAGC CTCGCCTCCT CCTCGTCAAC GACCCGGTTG TCGACCGTCA GGCTATTCTC GAGGCGTCCT ACGTGAATAT TCCGACTATA TCGCTTTGCA ACTCGGACGC TAACCTGAAG TTCATTGACG TGGCAATCCC CTGCAACAAC AAGACTCCCA TGAGCATCGG GCTTATTTAC TGGCTCCTTG CTCGTGAAGT TCTCAGGCTC AAGGGGAGCA TCTCAAGGAC TGAGGAGTGG GATGTGAAGC CCGATCTGTT TGTTGCTCTT CCCGAGGAAA TCCCAGACGA GGAGGAGAGT GAGGACTTCT ATGATGACGA TGAGGAGGAG GATGAGGAAT TTTCCGCCGG CAATGGCAAC CTCTTCGACG AGTAC

Details for GilaA.01085.a.A1.GE25720

HARVESTED ON:	10/31/2008
SEQUENCED ON:	7/20/2010
EXPECTED MW:	30kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	91

Validated AA Sequence

Validated NT Sequence

tggaagctca gacccagggt cctggttcga tgtcgacaga gaaaacgtcc caggcgtcca aggagtacca gctcaaggag gctgatgtga agaagatgct cgttgcaacg actcaccttg gcgtccgcaa catcgatagg aggatgcagt tctacatctt cgacaggcag aaggatggga cctttgtctt caacctgcag aaagtctggg ccaagatcgt cttcgcagca cgcatcctcg tcaccatcga tgaccccgcc gagatcgccg tggtcgctaa cagacccgat gctcagcgtg ctatcctcaa gttctgcaag tacacgcacg cgactgcctt ccccggccgc tttatccctg gcaacttcac caatcgtatg aacccgaact attgtgagcc tcgcctcctc ctcgtcaacg acccggttgt cgaccgtcag gctattctcg aggcgtccta cgtgaatatt ccgactatat cgctttgcaa ctcggacgct aacctgaagt tcattgacgt ggcaatcccc tgcaacaaca agactcccat gagcatcggg cttatttact ggctccttgc tcgtgaagtt ctcaggctca aggggagcat ctcaaggact gaggagtggg atgtgaagcc cgatctgttt gttgctcttc ccgaggaaat cccagacgag gaggagagtg aggacttcta tgatgacgat gaggaggagg atgaggaatt ttccgccggc aatggcaacc tcttcgacg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSTEKTSQA SKEYQLKEAD VKKMLVATTH LGVRNIDRRM QFYIFDRQKD GTFVFNLQKV WAKIVFAARI LVTIDDPAEI AVVANRPDAQ RAILKFCKYT HATAFPGRFI PGNFTNRMNP NYCEPRLLLV NDPVVDRQAI LEASYVNIPT ISLCNSDANL KFIDVAIPCN NKTPMSIGLI YWLLAREVLR LKGSISRTEE WDVKPDLFVA LPEEIPDEEE SEDFYDDDEE EDEEFSAGNG NLFDEY

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgacagag aaaacgtccc aggcgtccaa ggagtaccag ctcaaggagg ctgatgtgaa gaagatgctc gttgcaacga ctcaccttgg cgtccgcaac atcgatagga ggatgcagtt ctacatcttc gacaggcaga aggatgggac ctttgtcttc aacctgcaga aagtctgggc caagatcgtc ttcgcagcac gcatcctcgt caccatcgat gaccccgccg agatcgccgt ggtcgctaac agacccgatg ctcagcgtgc tatcctcaag ttctgcaagt acacgcacgc gactgccttc cccggccgct ttatccctgg caacttcacc aatcgtatga acccgaacta ttgtgagcct cgcctcctcc tcgtcaacga cccggttgtc gaccgtcagg ctattctcga ggcgtcctac gtgaatattc cgactatatc gctttgcaac tcggacgcta acctgaagtt cattgacgtg gcaatcccct gcaacaacaa gactcccatg agcatcgggc ttatttactg gctccttgct cgtgaagttc tcaggctcaa ggggagcatc tcaaggactg aggagtggga tgtgaagccc gatctgtttg ttgctcttcc cgaggaaatc ccagacgagg aggagagtga ggacttctat gatgacgatg aggaggagga tgaggaattt tccgccggca atggcaacct cttcgacgag tacaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa