SSGCID: Target Page

GilaA.01017.a

Deoxynucleoside kinase

CENTER ID:	GilaA.01017.a
ORGANISM:	Giardia lamblia ATCC 50803
ASSOCIATED DISEASE:	Giardiasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
GilaA.01017.a.A1.GE25689	Full length( GilaA.01017.a )		1	251

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	GiardiaDB:GL50803_17451
RefSeq:	XP_001708575.1
UniProt:	A8B9V6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAARNLDATN QKLDPQVGKK DDDMGFPYPK MFISISGLIG AGKTTLAEEL AKEMGFPVYY EPVEDNIYLE DFYKDMKTYG FPMQVYLLNK RYQQHQQIIW SGEGAVQDRS IYEDAVFARI LVKQGNMSKR DYKTYTDLYN NMSKYLTHPN FLIHLDVTPE ESLERIKERS RNCEAGIPLS YLQDLYDEYN IFLAEISKRI PVIRIRWANY RSAKELAMRV YAEMKKHQAI VDVPFDGTMT APIPQSSSNF Y

NT Sequence

ATGGCTGCGC GAAATCTGGA TGCAACCAAT CAAAAGTTGG ACCCACAGGT AGGTAAAAAG GATGATGACA TGGGGTTTCC GTATCCAAAG ATGTTCATCT CTATATCAGG GCTGATAGGT GCTGGAAAGA CGACCTTGGC AGAAGAGCTC GCCAAGGAGA TGGGATTTCC TGTTTACTAC GAGCCCGTTG AAGATAACAT CTATTTGGAA GACTTCTACA AAGACATGAA GACGTATGGC TTCCCCATGC AGGTTTACCT ACTCAACAAA CGTTACCAAC AGCACCAGCA AATCATCTGG AGTGGAGAGG GCGCCGTACA GGATAGATCC ATATACGAAG ACGCCGTCTT TGCGCGCATT CTTGTGAAGC AGGGGAACAT GTCGAAGCGC GACTATAAGA CCTACACCGA TCTATACAAC AATATGAGCA AGTACTTGAC ACACCCAAAC TTTCTGATTC ACTTAGATGT CACACCCGAA GAATCCTTGG AAAGAATCAA GGAAAGATCA CGCAACTGTG AAGCAGGAAT ACCCCTTTCT TATTTGCAGG ACCTCTACGA TGAATATAAC ATTTTCCTTG CCGAGATATC CAAAAGAATC CCAGTAATAC GCATAAGGTG GGCCAACTAT CGCAGCGCAA AGGAGCTGGC CATGCGCGTG TATGCCGAGA TGAAGAAACA CCAAGCCATC GTAGACGTCC CGTTCGACGG AACGATGACA GCTCCCATTC CTCAATCTTC TTCCAATTTC TAT

Details for GilaA.01017.a.A1.GE25689

HARVESTED ON:	10/17/2008
SEQUENCED ON:	7/20/2010
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAARNLDAT NQKLDPQVGK KDDDMGFPYP KMFISISGLI GAGKTTLAEE LAKEMGFPVY YEPVEDNIYL EDFYKDMKTY GFPMQVYLLN KRYQQHQQII WSGEGAVQDR SIYEDAVFAR ILVKQGNMSK RDYKTYTDLY NNMSKYLTHP NFLIHLDVTP EESLERIKER SRNCEAGIPL SYLQDLYDEY NIFLAEISKR IPVIRIRWAN YRSAKELAMR VYAEMKKHQA IVDVPFDGTM TAPIPQSSSN FY

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggctg cgcgaaatct ggatgcaacc aatcaaaagt tggacccaca ggtaggtaaa aaggatgatg acatggggtt tccgtatcca aagatgttca tctctatatc agggctgata ggtgctggaa agacgacctt ggcagaagag ctcgccaagg agatgggatt tcctgtttac tacgagcccg ttgaagataa catctatttg gaagacttct acaaagacat gaagacgtat ggcttcccca tgcaggttta cctactcaac aaacgttacc aacagcacca gcaaatcatc tggagtggag agggcgccgt acaggataga tccatatacg aagacgccgt ctttgcgcgc attcttgtga agcaggggaa catgtcgaag cgcgactata agacctacac cgatctatac aacaatatga gcaagtactt gacacaccca aactttctga ttcacttaga tgtcacaccc gaagaatcct tggaaagaat caaggaaaga tcacgcaact gtgaagcagg aatacccctt tcttatttgc aggacctcta cgatgaatat aacattttcc ttgccgagat atccaaaaga atcccagtaa tacgcataag gtgggccaac tatcgcagcg caaaggagct ggccatgcgc gtgtatgccg agatgaagaa acaccaagcc atcgtagacg tcccgttcga cggaacgatg acagctccca ttcctcaatc ttcttccaat ttctattaat aa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgcgcga aatctggatg caaccaatca aaagttggac ccacaggtag gtaaaaagga tgatgacatg gggtttccgt atccaaagat gttcatctct atatcagggc tgataggtgc tggaaagacg accttggcag aagagctcgc caaggagatg ggatttcctg tttactacga gcccgttgaa gataacatct atttggaaga cttctacaaa gacatgaaga cgtatggctt ccccatgcag gtttacctac tcaacaaacg ttaccaacag caccagcaaa tcatctggag tggagagggc gccgtacagg atagatccat atacgaagac gccgtctttg cgcgcattct tgtgaagcag gggaacatgt cgaagcgcga ctataagacc tacaccgatc tatacaacaa tatgagcaag tacttgacac acccaaactt tctgattcac ttagatgtca cacccgaaga atccttggaa agaatcaagg aaagatcacg caactgtgaa gcaggaatac ccctttctta tttgcaggac ctctacgatg aatataacat tttccttgcc gagatatcca aaagaatccc agtaatacgc ataaggtggg ccaactatcg cagcgcaaag gagctggcca tgcgcgtgta tgccgagatg aagaaacacc aagccatcgt agacgtcccg ttcgacggaa cgatgacagc tcccattcct caatcttctt ccaatttcta taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa