GilaA.00988.a

Arsenical pump-driving ATPase

CENTER ID:	GilaA.00988.a
ORGANISM:	Giardia lamblia ATCC 50803
ASSOCIATED DISEASE:	Giardiasis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
GilaA.00988.a.A1.GE25685	Full length( GilaA.00988.a )		1	354

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	GiardiaDB:GL50803_7953
OrthoMCL:	OG5_127181
RefSeq:	XP_001710217.1
UniProt:	A8B3G9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLPSLHDILD QHTYKWIFFG GKGGVGKTTT SSSFSVLMAE TRPNEKFLLL STDPAHNISD AFDQKFGKAP TQVSGIPNLY AMEVDASNEM KSAVEAVQKE TGSAADNDAE SKSEGDMFGG LNDLITCASS FIKDGTFPGM DEMWSFINLI KLIDTNEYST VIFDTAPTGH TLRFLELPET VNKVLEIFTR LKDNMGGMLS MVMQTMGLSQ NDIFGLIDKT YPKIDVVKRI SAEFRDPSLC TFVGVCIPEF LSLYETERLV QRLAVLDMDC HAIVINFVLD ANAATPCSMC RSRARMQNKY IDQINELYDD FNIVLSPLRH DEVRGIANLR DYAETLIKPY RFCWSANPDP SSAK

NT Sequence

ATGCTCCCAT CGCTCCACGA CATACTAGAC CAGCACACAT ACAAATGGAT ATTCTTTGGG GGAAAGGGTG GCGTTGGGAA GACAACCACC TCTTCTTCGT TTTCTGTCCT CATGGCCGAG ACCCGTCCTA ATGAGAAATT CCTGCTCTTG TCAACAGATC CTGCGCACAA CATTTCCGAT GCCTTTGACC AGAAGTTTGG CAAGGCTCCG ACTCAAGTCA GCGGGATTCC CAACCTGTAT GCAATGGAGG TCGATGCCTC TAATGAGATG AAGTCTGCAG TTGAAGCCGT TCAAAAGGAG ACAGGCTCTG CCGCTGACAA TGATGCTGAG TCCAAATCTG AAGGCGACAT GTTTGGCGGC CTCAACGACC TAATAACATG TGCCTCCTCG TTTATTAAAG ACGGAACGTT CCCCGGGATG GATGAGATGT GGTCCTTCAT TAACTTAATT AAGCTCATAG ATACCAATGA GTACTCTACA GTTATCTTTG ATACGGCGCC CACAGGCCAC ACTCTGCGCT TCCTCGAGTT GCCAGAAACG GTAAACAAGG TGCTAGAAAT CTTCACCAGA TTGAAGGATA ACATGGGAGG GATGCTTTCG ATGGTAATGC AAACCATGGG ACTGTCTCAG AACGATATCT TTGGCCTCAT TGACAAGACT TACCCGAAGA TCGACGTCGT CAAAAGGATC TCTGCTGAGT TTCGGGACCC ATCCCTGTGC ACCTTTGTAG GGGTCTGCAT ACCGGAGTTT CTATCTCTCT ATGAGACAGA GAGGCTTGTT CAGCGGCTTG CAGTCCTGGA CATGGATTGT CACGCCATAG TTATCAACTT TGTGCTCGAT GCAAATGCTG CCACTCCTTG CTCCATGTGC AGGAGTCGGG CGCGAATGCA GAATAAGTAT ATTGACCAAA TCAATGAGCT CTATGACGAC TTCAATATTG TTCTTAGTCC ACTGAGACAC GATGAAGTGC GGGGGATAGC CAATCTTCGT GACTACGCCG AGACACTCAT CAAGCCCTAC AGGTTCTGTT GGTCTGCGAA CCCAGACCCG TCCAGTGCGA AG

Details for GilaA.00988.a.A1.GE25685

HARVESTED ON:	10/17/2008
SEQUENCED ON:	7/20/2010
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLPSLHDIL DQHTYKWIFF GGKGGVGKTT TSSSFSVLMA ETRPNEKFLL LSTDPAHNIS DAFDQKFGKA PTQVSGIPNL YAMEVDASNE MKSAVEAVQK ETGSAADNDA ESKSEGDMFG GLNDLITCAS SFIKDGTFPG MDEMWSFINL IKLIDTNEYS TVIFDTAPTG HTLRFLELPE TVNKVLEIFT RLKDNMGGML SMVMQTMGLS QNDIFGLIDK TYPKIDVVKR ISAEFRDPSL CTFVGVCIPE FLSLYETERL VQRLAVLDMD CHAIVINFVL DANAATPCSM CRSRARMQNK YIDQINELYD DFNIVLSPLR HDEVRGIANL RDYAETLIKP YRFCWSANPD PSSAK

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctcc catcgctcca cgacatacta gaccagcaca catacaaatg gatattcttt gggggaaagg gtggcgttgg gaagacaacc acctcttctt cgttttctgt cctcatggcc gagacccgtc ctaatgagaa attcctgctc ttgtcaacag atcctgcgca caacatttcc gatgcctttg accagaagtt tggcaaggct ccgactcaag tcagcgggat tcccaacctg tatgcaatgg aggtcgatgc ctctaatgag atgaagtctg cagttgaagc cgttcaaaag gagacaggct ctgccgctga caatgatgct gagtccaaat ctgaaggcga catgtttggc ggcctcaacg acctaataac atgtgcctcc tcgtttatta aagacggaac gttccccggg atggatgaga tgtggtcctt cattaactta attaagctca tagataccaa tgagtactct acagttatct ttgatacggc gcccacaggc cacactctgc gcttcctcga gttgccagaa acggtaaaca aggtgctaga aatcttcacc agattgaagg ataacatggg agggatgctt tcgatggtaa tgcaaaccat gggactgtct cagaacgata tctttggcct cattgacaag acttacccga agatcgacgt cgtcaaaagg atctctgctg agtttcggga cccatccctg tgcacctttg taggggtctg cataccggag tttctatctc tctatgagac agagaggctt gttcagcggc ttgcagtcct ggacatggat tgtcacgcca tagttatcaa ctttgtgctc gatgcaaatg ctgccactcc ttgctccatg tgcaggagtc gggcgcgaat gcagaataag tatattgacc aaatcaatga gctctatgac gacttcaata ttgttcttag tccactgaga cacgatgaag tgcgggggat agccaatctt cgtgactacg ccgagacact catcaagccc tacaggttct gttggtctgc gaacccagac ccgtccagtg cgaagtaata a

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctcccatcg ctccacgaca tactagacca gcacacatac aaatggatat tctttggggg aaagggtggc gttgggaaga caaccacctc ttcttcgttt tctgtcctca tggccgagac ccgtcctaat gagaaattcc tgctcttgtc aacagatcct gcgcacaaca tttccgatgc ctttgaccag aagtttggca aggctccgac tcaagtcagc gggattccca acctgtatgc aatggaggtc gatgcctcta atgagatgaa gtctgcagtt gaagccgttc aaaaggagac aggctctgcc gctgacaatg atgctgagtc caaatctgaa ggcgacatgt ttggcggcct caacgaccta ataacatgtg cctcctcgtt tattaaagac ggaacgttcc ccgggatgga tgagatgtgg tccttcatta acttaattaa gctcatagat accaatgagt actctacagt tatctttgat acggcgccca caggccacac tctgcgcttc ctcgagttgc cagaaacggt aaacaaggtg ctagaaatct tcaccagatt gaaggataac atgggaggga tgctttcgat ggtaatgcaa accatgggac tgtctcagaa cgatatcttt ggcctcattg acaagactta cccgaagatc gacgtcgtca aaaggatctc tgctgagttt cgggacccat ccctgtgcac ctttgtaggg gtctgcatac cggagtttct atctctctat gagacagaga ggcttgttca gcggcttgca gtcctggaca tggattgtca cgccatagtt atcaactttg tgctcgatgc aaatgctgcc actccttgct ccatgtgcag gagtcgggcg cgaatgcaga ataagtatat tgaccaaatc aatgagctct atgacgactt caatattgtt cttagtccac tgagacacga tgaagtgcgg gggatagcca atcttcgtga ctacgccgag acactcatca agccctacag gttctgttgg tctgcgaacc cagacccgtc cagtgcgaag aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa