GilaA.00722.a

Kinase, NEK

CENTER ID: GilaA.00722.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.00722.a.A1.GE29775 Full length( GilaA.00722.a ) 1 303
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_9658
RefSeq: XP_001704008.1
UniProt: A8BZI8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKFEEHYVS TGPVLAKTAL GHVEVVRSLD PACNDGSQGQ DYAVKVVNIT SIPNRYRSVV RNDFIKLCSL SHKNLVKYYD VYYNDTKNLG HFVMEYYERC CLMDVILFYR MKERVIPEET VWYILSHLAE ALLYYHSPQK DNTDMGPLVH RNIKPSKVFL AADGYCKLAD SGTFRLLDNV ILSSTIFGTL GFCPPEVLKM ERYTEESDLW SLGALVYCVC ALKPPAYSAN PEELLKKLQG FEVDVNELRE REYSEELIGT LQSMLNYDPK QRIDLSKLNA IPQIVDKKGP IKSSTWGLNF SSK
NT Sequence
ATGGCCAAGT TCGAGGAGCA TTATGTCTCC ACTGGCCCCG TCCTAGCAAA GACGGCTTTG GGACACGTGG AAGTGGTACG GTCACTGGAC CCAGCGTGCA ATGATGGAAG TCAAGGGCAA GACTATGCAG TCAAGGTAGT CAATATTACA AGTATACCGA ACAGGTACAG ATCTGTCGTA CGCAACGACT TCATCAAGCT ATGCTCACTG TCACACAAGA ACCTTGTGAA GTATTACGAT GTTTATTATA ATGATACGAA AAACCTTGGT CATTTTGTAA TGGAGTATTA CGAAAGGTGC TGCCTAATGG ATGTGATCCT CTTTTACAGA ATGAAGGAGC GCGTGATCCC TGAGGAAACG GTGTGGTATA TTCTCTCTCA CCTAGCAGAG GCTCTTCTTT ATTACCATTC TCCACAGAAG GACAATACCG ACATGGGTCC CCTAGTACAC AGAAACATTA AGCCATCGAA GGTGTTTCTA GCAGCCGACG GATACTGCAA ACTCGCTGAC TCCGGAACGT TTAGACTGCT GGACAATGTA ATATTGAGCT CTACCATATT CGGCACCCTG GGATTTTGTC CCCCAGAGGT CCTCAAAATG GAGAGATACA CAGAAGAAAG CGATCTATGG TCCCTAGGCG CTCTTGTGTA TTGTGTGTGT GCTCTTAAGC CGCCTGCATA CTCTGCAAAC CCCGAGGAGC TACTGAAAAA GCTGCAAGGC TTTGAGGTAG ACGTGAATGA ATTAAGAGAG CGGGAGTATA GCGAGGAGCT GATCGGAACG CTACAGAGCA TGTTAAACTA CGACCCTAAG CAACGGATTG ATCTATCTAA ACTGAACGCG ATACCCCAAA TTGTCGACAA AAAGGGGCCA ATCAAGAGTT CCACGTGGGG ACTAAACTTT TCAAGTAAG
Details for GilaA.00722.a.A1.GE29775
HARVESTED ON: 5/27/2010
SEQUENCED ON: 11/24/2008
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: failed alignment
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAKFEEHYV STGPVLAKTA LGHVEVVRSL DPACNDGSQG QDYAVKVVNI TSIPNRYRSV VRNDFIKLCS LSHKNLVKYY DVYYNDTKNL GHFVMEYYER CCLMDVILFY RMKERVIPEE TVWYILSHLA EALLYYHSPQ KDNTDMGPLV HRNIKPSKVF LAADGYCKLA DSGTFRLLDN VILSSTIFGT LGFCPPEVLK MERYTEESDL WSLGALVYCV CALKPPAYSA NPEELLKKLQ GFEVDVNELR EREYSEELIG TLQSMLNYDP KQRIDLSKLN AIPQIVDKKG PIKSSTWGLN FSSK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcca agttcgagga gcattatgtc tccactggcc ccgtcctagc aaagacggct ttgggacacg tggaagtggt acggtcactg gacccagcgt gcaatgatgg aagtcaaggg caagactatg cagtcaaggt agtcaatatt acaagtatac cgaacaggta cagatctgtc gtacgcaacg acttcatcaa gctatgctca ctgtcacaca agaaccttgt gaagtattac gatgtttatt ataatgatac gaaaaacctt ggtcattttg taatggagta ttacgaaagg tgctgcctaa tggatgtgat cctcttttac agaatgaagg agcgcgtgat ccctgaggaa acggtgtggt atattctctc tcacctagca gaggctcttc tttattacca ttctccacag aaggacaata ccgacatggg tcccctagta cacagaaaca ttaagccatc gaaggtgttt ctagcagccg acggatactg caaactcgct gactccggaa cgtttagact gctggacaat gtaatattga gctctaccat attcggcacc ctgggatttt gtcccccaga ggtcctcaaa atggagagat acacagaaga aagcgatcta tggtccctag gcgctcttgt gtattgtgtg tgtgctctta agccgcctgc atactctgca aaccccgagg agctactgaa aaagctgcaa ggctttgagg tagacgtgaa tgaattaaga gagcgggagt atagcgagga gctgatcgga acgctacaga gcatgttaaa ctacgaccct aagcaacgga ttgatctatc taaactgaac gcgatacccc aaattgtcga caaaaagggg ccaatcaaga gttccacgtg gggactaaac ttttcaagta agtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAKFEEHYV STGPVLAKTA LGHVEVVRSL DPACNDGSQG QDYAVKVVNI TSIPNRYRSV VRNDFIKLCS LSHKNLVKYY DVYYNDTKNL GHFVMEYYER CCLMDVILFY RMKERVIPEE TVWYILSHLA EALLYYHSPQ KDNTDMGPLV HRNIKPSKVF LAADGYCKLA DSGTFRLLDN VILSSTIFGT LGFCPPEVLK MERYTEESDL WSLGALVYCV CALKPPAYSA NPEELLKKLQ GFEVDVNELR EREYSEELIG TLQSMLNYDP KQRIDLSKLN AIPQIVDKKG PIKSSTWGLN FSSK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccaagttc gaggagcatt atgtctccac tggccccgtc ctagcaaaga cggctttggg acacgtggaa gtggtacggt cactggaccc agcgtgcaat gatggaagtc aagggcaaga ctatgcagtc aaggtagtca atattacaag tataccgaac aggtacagat ctgtcgtacg caacgacttc atcaagctat gctcactgtc acacaagaac cttgtgaagt attacgatgt ttattataat gatacgaaaa accttggtca ttttgtaatg gagtattacg aaaggtgctg cctaatggat gtgatcctct tttacagaat gaaggagcgc gtgatccctg aggaaacggt gtggtatatt ctctctcacc tagcagaggc tcttctttat taccattctc cacagaagga caataccgac atgggtcccc tagtacacag aaacattaag ccatcgaagg tgtttctagc agccgacgga tactgcaaac tcgctgactc cggaacgttt agactgctgg acaatgtaat attgagctct accatattcg gcaccctggg attttgtccc ccagaggtcc tcaaaatgga gagatacaca gaagaaagcg atctatggtc cctaggcgct cttgtgtatt gtgtgtgtgc tcttaagccg cctgcatact ctgcaaaccc cgaggagcta ctgaaaaagc tgcaaggctt tgaggtagac gtgaatgaat taagagagcg ggagtatagc gaggagctga tcggaacgct acagagcatg ttaaactacg accctaagca acggattgat ctatctaaac tgaacgcgat accccaaatt gtcgacaaaa aggggccaat caagagttcc acgtggggac taaacttttc aagtaagaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa