GilaA.00634.a

Rab GDI

CENTER ID: GilaA.00634.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.00634.a.A1.GE25656 Full length( GilaA.00634.a ) 1 471
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_11495
RefSeq: XP_001706989.1
UniProt: A8BGT6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDTLPKEFDA IVLGTGLKEG IVSALLSVHG RKVLHIDRND FYGGDCASLK LSQLYSFFGE SLSSIPAEFG KDNEWSIDLI PKFILSSGDL FYMLRHVDCL HYLEFGRVAG AFVYNNGVIH RVPATTKQAL DSKLMGLFEK KRMANLFEYI TSFEENPSAT SNLSSQGKTP ATATCNEYFD AYKLSDSTKE FIGHAMALEL DDEYLNKPAL ETFRRIDLYT QSLSRFGQSP FIYPLYGLGD LPQAFSRVAA VWGGIYMLNT PVSEILYNDD KKVRGVRFGD HEVSAPIVVG DPSYFPDLVV SKGRVGRAIC ILKGPIPECD QQSDVEKDGA GNRIVQTAHQ VILPASQIQR KSDIYVSTQG YNHRTTPRNC VLGYVSGLVY GSRSDNRDEL KAGFDILKRA GISKTFYKEY DLLEPKEEAQ RRNIFISKSY DATSHFESTV ADAIDMYQRI TGETLDLTTI VKRQAPTVED A
NT Sequence
ATGGACACCC TTCCCAAGGA GTTCGATGCC ATAGTTTTGG GCACAGGTCT GAAAGAGGGG ATTGTCTCTG CTCTCCTGTC TGTCCACGGG CGTAAGGTCC TTCACATTGA TCGAAATGAC TTCTACGGAG GAGATTGTGC GTCATTGAAA CTCTCGCAAC TTTACTCTTT TTTTGGTGAA AGTCTGTCGT CAATCCCCGC AGAGTTCGGA AAGGACAACG AATGGAGCAT AGACCTTATA CCTAAGTTCA TACTCTCCAG CGGAGACTTA TTCTACATGC TTCGTCATGT TGATTGTCTT CACTATCTGG AGTTTGGGCG TGTCGCAGGT GCGTTTGTTT ACAATAACGG TGTCATTCAT CGTGTGCCCG CCACAACCAA GCAAGCTCTG GACTCAAAAC TTATGGGTCT GTTTGAGAAA AAGCGTATGG CAAACCTTTT CGAGTATATT ACTAGCTTTG AAGAGAATCC ATCTGCTACC TCCAATTTAT CTAGCCAGGG AAAGACACCT GCAACTGCTA CTTGTAACGA ATACTTTGAT GCATATAAGC TTTCGGATTC GACTAAGGAG TTCATTGGAC ATGCAATGGC TTTAGAGCTG GATGATGAGT ATTTGAACAA GCCAGCTTTG GAGACCTTCC GCCGCATCGA TCTCTACACG CAGAGCCTCA GCCGCTTTGG CCAGTCTCCC TTCATCTATC CTCTCTACGG CCTGGGAGAC CTCCCGCAGG CGTTCAGCAG GGTTGCAGCC GTGTGGGGAG GAATATACAT GCTCAATACC CCTGTTTCTG AAATTTTGTA CAACGATGAC AAGAAGGTTC GTGGAGTACG ATTCGGAGAC CATGAAGTTT CTGCCCCAAT CGTCGTGGGA GATCCCAGCT ATTTTCCTGA CCTTGTTGTG AGCAAGGGAC GCGTGGGCCG GGCGATATGC ATATTAAAAG GCCCTATTCC GGAATGTGAC CAGCAGTCCG ACGTGGAGAA GGATGGCGCT GGAAATAGAA TCGTGCAAAC CGCCCACCAA GTCATTCTTC CAGCCTCTCA GATACAACGC AAATCAGATA TCTATGTCTC GACGCAGGGA TACAACCATC GAACTACGCC ACGCAACTGT GTGCTGGGCT ATGTCTCAGG CCTTGTCTAT GGAAGTAGGA GTGACAACCG CGATGAGCTC AAGGCAGGAT TTGATATTCT CAAGAGAGCA GGAATCAGTA AGACCTTTTA CAAAGAGTAT GACCTATTAG AACCAAAGGA GGAGGCTCAG AGGAGAAATA TCTTTATCAG CAAGTCTTAC GACGCTACGT CCCACTTCGA GAGCACAGTA GCTGATGCAA TAGACATGTA TCAACGCATT ACAGGAGAGA CTCTAGATCT CACTACCATT GTGAAGCGTC AGGCTCCCAC AGTGGAGGAT GCG
Details for GilaA.00634.a.A1.GE25656
HARVESTED ON: 10/31/2008
SEQUENCED ON: 7/20/2010
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 95
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDTLPKEFD AIVLGTGLKE GIVSALLSVH GRKVLHIDRN DFYGGDCASL KLSQLYSFFG ESLSSIPAEF GKDNEWSIDL IPKFILSSGD LFYMLRHVDC LHYLEFGRVA GAFVYNNGVI HRVPATTKQA LDSKLMGLFE KKRMANLFEY ITSFEENPSA TSNLSSQGKT PATATCNEYF DAYKLSDSTK EFIGHAMALE LDDEYLNKPA LETFRRIDLY TQSLSRFGQS PFIYPLYGLG DLPQAFSXVA AVWGGIYMLN TPVSEILYND DKXXRXXRFX DHEXSAXXVV GDPSYFPXXV VSKGRVGRAI CIXXXXIPEC DQQSXVEKDG AGNRIVQTAH XVILPAXQIQ RKSDIYVSTX XYNHRTTPRN CVLGYVSGLV YGSRSDNRDE LKAGFDILKR AGISKTFYKE YDLLEPKEEA QRRNIFISKS YDATSHFEST VADAIDMYQR ITGETLDLTT IVKRQAPTVE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaca cccttcccaa ggagttcgat gccatagttt tgggcacagg tctgaaagag gggattgtct ctgctctcct gtctgtccac gggcgtaagg tccttcacat tgatcgaaat gacttctacg gaggagattg tgcgtcattg aaactctcgc aactttactc tttttttggt gaaagtctgt cgtcaatccc cgcagagttc ggaaaggaca acgaatggag catagacctt atacctaagt tcatactctc cagcggagac ttattctaca tgcttcgtca tgttgattgt cttcactatc tggagtttgg gcgtgtcgca ggtgcgtttg tttacaataa cggtgtcatt catcgtgtgc ccgccacaac caagcaagct ctggactcaa aacttatggg tctgtttgag aaaaagcgta tggcaaacct tttcgagtat attactagct ttgaagagaa tccatctgct acctccaatt tatctagcca gggaaagaca cctgcaactg ctacttgtaa cgaatacttt gatgcatata agctttcgga ttcgactaag gagttcattg gacatgcaat ggctttagag ctggatgatg agtatttgaa caagccagct ttggagacct tccgccgcat cgatctctac acgcagagcc tcagccgctt tggccagtct cccttcatct atcctctcta cggcctggga gacctcccgc aggcgttcag canggttgca gccgtgtggg gaggaatata catgctcaat acccctgttt ctgaaatttt gtacaacgat gacaagannn ntcgtgnnnn acgattcnna gaccatgaan tttctgcccn nntcgtcgtg ggagatccca gctattttcc tgnnnttgtt gtgagcaagg gacgcgtggg ccgggcgata tgcatatnna angnccntat tccggaatgt gaccagcagt cnnncgtgga gaaggatggc gctggaaata gaatcgtgca aaccgcccac cnagtcattc ttccagcctn tcagatacaa cgcaaatcag atatctatgt ctcgacgcnn ngatacaacc atcgaactac gccacgcaac tgtgtgctgg gctatgtctc aggccttgtc tatggaagta ggagtgacaa ccgcgatgag ctcaaggcag gatttgatat tctcaagaga gcaggaatca gtaagacctt ttacaaagag tatgacctat tagaaccaaa ggaggaggct cagaggagaa atatctttat cagcaagtct tacgacgcta cgtcccactt cgagagcaca gtagctgatg caatagacat gtatcaacgc attacaggag agactctaga tctcactacc attgtgaagc gtcaggctcc cacagtggag g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDTLPKEFD AIVLGTGLKE GIVSALLSVH GRKVLHIDRN DFYGGDCASL KLSQLYSFFG ESLSSIPAEF GKDNEWSIDL IPKFILSSGD LFYMLRHVDC LHYLEFGRVA GAFVYNNGVI HRVPATTKQA LDSKLMGLFE KKRMANLFEY ITSFEENPSA TSNLSSQGKT PATATCNEYF DAYKLSDSTK EFIGHAMALE LDDEYLNKPA LETFRRIDLY TQSLSRFGQS PFIYPLYGLG DLPQAFSRVA AVWGGIYMLN TPVSEILYND DKKVRGVRFG DHEVSAPIVV GDPSYFPDLV VSKGRVGRAI CILKGPIPEC DQQSDVEKDG AGNRIVQTAH QVILPASQIQ RKSDIYVSTQ GYNHRTTPRN CVLGYVSGLV YGSRSDNRDE LKAGFDILKR AGISKTFYKE YDLLEPKEEA QRRNIFISKS YDATSHFEST VADAIDMYQR ITGETLDLTT IVKRQAPTVE DA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaccctt cccaaggagt tcgatgccat agttttgggc acaggtctga aagaggggat tgtctctgct ctcctgtctg tccacgggcg taaggtcctt cacattgatc gaaatgactt ctacggagga gattgtgcgt cattgaaact ctcgcaactt tactcttttt ttggtgaaag tctgtcgtca atccccgcag agttcggaaa ggacaacgaa tggagcatag accttatacc taagttcata ctctccagcg gagacttatt ctacatgctt cgtcatgttg attgtcttca ctatctggag tttgggcgtg tcgcaggtgc gtttgtttac aataacggtg tcattcatcg tgtgcccgcc acaaccaagc aagctctgga ctcaaaactt atgggtctgt ttgagaaaaa gcgtatggca aaccttttcg agtatattac tagctttgaa gagaatccat ctgctacctc caatttatct agccagggaa agacacctgc aactgctact tgtaacgaat actttgatgc atataagctt tcggattcga ctaaggagtt cattggacat gcaatggctt tagagctgga tgatgagtat ttgaacaagc cagctttgga gaccttccgc cgcatcgatc tctacacgca gagcctcagc cgctttggcc agtctccctt catctatcct ctctacggcc tgggagacct cccgcaggcg ttcagcaggg ttgcagccgt gtggggagga atatacatgc tcaatacccc tgtttctgaa attttgtaca acgatgacaa gaaggttcgt ggagtacgat tcggagacca tgaagtttct gccccaatcg tcgtgggaga tcccagctat tttcctgacc ttgttgtgag caagggacgc gtgggccggg cgatatgcat attaaaaggc cctattccgg aatgtgacca gcagtccgac gtggagaagg atggcgctgg aaatagaatc gtgcaaaccg cccaccaagt cattcttcca gcctctcaga tacaacgcaa atcagatatc tatgtctcga cgcagggata caaccatcga actacgccac gcaactgtgt gctgggctat gtctcaggcc ttgtctatgg aagtaggagt gacaaccgcg atgagctcaa ggcaggattt gatattctca agagagcagg aatcagtaag accttttaca aagagtatga cctattagaa ccaaaggagg aggctcagag gagaaatatc tttatcagca agtcttacga cgctacgtcc cacttcgaga gcacagtagc tgatgcaata gacatgtatc aacgcattac aggagagact ctagatctca ctaccattgt gaagcgtcag gctcccacag tggaggatgc gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa