GilaA.00542.a

Kinase, CMGC RCK

CENTER ID: GilaA.00542.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.00542.a.A1.GE29774 Full length( GilaA.00542.a ) 1 302
GilaA.00542.a.A1.GE30657 Full length( GilaA.00542.a ) 1 302
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_14172
RefSeq: XP_001706190.1
UniProt: A8BLV8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDAYTPVRRV GSGTYGDVLH AVDTKGDFVA IKIMNSHTLC VSDPLQLPEV ATLRRLPPHP NIIRLIRVVV TRDHTHLIFE YAGGGTLLNF LRSSGPLKEH MLRNIAFRIL QGLYHIHTNS VIHRDLKLEN ILLTSETGDA NGDIVGVKIA DFGLSRAQGS RQNTDYVSTR WYRAPELLLH YSGYGPPIDI WAAGCCFAEL LTGDPLFPGD TEIQQLQLIC AFLKPLKPDA CKGAMHALPR FITENMTVSD ADIYTLAEAR EALRRELGCS HVLADLLAHM LAWNPEDRIT AQRALMHQFF AI
NT Sequence
ATGGATGCCT ACACGCCGGT GCGTCGCGTG GGGTCTGGCA CGTACGGGGA TGTCCTGCAC GCAGTGGATA CGAAGGGGGA TTTCGTCGCC ATCAAGATCA TGAACAGTCA CACCCTCTGT GTCTCTGATC CTCTCCAGCT GCCCGAAGTA GCTACGCTCC GGAGGCTTCC ACCCCATCCA AATATCATTC GCCTTATTCG AGTAGTTGTC ACTAGGGACC ACACACACTT GATCTTCGAA TACGCAGGTG GAGGTACCCT CCTGAATTTT CTTCGATCTT CGGGACCACT CAAAGAGCAC ATGCTACGAA ACATTGCATT CCGCATTCTC CAGGGCCTCT ATCACATCCA TACGAACAGC GTGATTCACC GGGATTTGAA ACTAGAGAAC ATTCTATTAA CGAGCGAGAC GGGAGATGCC AACGGAGACA TTGTTGGGGT AAAGATAGCT GACTTTGGCC TTTCAAGGGC ACAGGGATCC CGTCAAAATA CCGATTATGT TAGCACGCGG TGGTATAGAG CACCTGAGCT CCTTCTGCAC TATAGCGGAT ATGGCCCTCC AATCGACATC TGGGCAGCAG GTTGTTGCTT TGCTGAGCTG CTTACGGGCG ATCCTCTGTT CCCCGGCGAC ACCGAGATTC AGCAGCTGCA GCTCATCTGT GCCTTCCTAA AACCCCTAAA GCCGGATGCC TGCAAAGGCG CCATGCATGC CTTGCCTCGC TTCATAACCG AAAACATGAC AGTGTCAGAT GCCGACATTT ATACGCTAGC TGAAGCTAGA GAAGCTCTTC GCAGAGAGCT CGGCTGCAGT CATGTGTTGG CAGATCTCCT CGCACACATG CTTGCGTGGA ATCCTGAGGA CCGTATTACT GCTCAGCGCG CCCTTATGCA CCAGTTCTTC GCAATA
Details for GilaA.00542.a.A1.GE29774
HARVESTED ON: 5/27/2010
SEQUENCED ON: 11/24/2008
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: failed alignment
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDAYTPVRR VGSGTYGDVL HAVDTKGDFV AIKIMNSHTL CVSDPLQLPE VATLRRLPPH PNIIRLIRVV VTRDHTHLIF EYAGGGTLLN FLRSSGPLKE HMLRNIAFRI LQGLYHIHTN SVIHRDLKLE NILLTSETGD ANGDIVGVKI ADFGLSRAQG SRQNTDYVST RWYRAPELLL HYSGYGPPID IWAAGCCFAE LLTGDPLFPG DTEIQQLQLI CAFLKPLKPD ACKGAMHALP RFITENMTVS DADIYTLAEA REALRRELGC SHVLADLLAH MLAWNPEDRI TAQRALMHQF FAI
Validated NT Sequence
ttattatatt gcgaagaact ggtgcataag ggcgcgctga gcagtaatac ggtcctcagg attccacgca agcatgtgtg cgaggagatc tgccaacaca tgactgcagc cgagctctct gcgaagagct tctctagctt cagctagcgt ataaatgtcg gcatctgaca ctgtcatgtt ttcggttatg aagcgaggca aggcatgcat ggcgcctttg caggcatccg gctttagggg ttttaggaag gcacagatga gctgcagctg ctgaatctcg gtgtcgccgg ggaacagagg atcgcccgta agcagctcag caaagcaaca acctgctgcc cagatgtcga ttggagggcc atatccgcta tagtgcagaa ggagctcagg tgctctatac caccgcgtgc taacataatc ggtattttga cgggatccct gtgcccttga aaggccaaag tcagctatct ttaccccaac aatgtctccg ttggcatctc ccgtctcgct cgttaataga atgttctcta gtttcaaatc ccggtgaatc acgctgttcg tatggatgtg atagaggccc tggagaatgc ggaatgcaat gtttcgtagc atgtgctctt tgagtggtcc cgaagatcga agaaaattca ggagggtacc tccacctgcg tattcgaaga tcaagtgtgt gtggtcccta gtgacaacta ctcgaataag gcgaatgata tttggatggg gtggaagcct ccggagcgta gctacttcgg gcagctggag aggatcagag acacagaggg tgtgactgtt catgatcttg atggcgacga aatccccctt cgtatccact gcgtgcagga catccccgta cgtgccagac cccacgcgac gcaccggcgt gtaggcatcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDAYTPVRR VGSGTYGDVL HAVDTKGDFV AIKIMNSHTL CVSDPLQLPE VATLRRLPPH PNIIRLIRVV VTRDHTHLIF EYAGGGTLLN FLRSSGPLKE HMLRNIAFRI LQGLYHIHTN SVIHRDLKLE NILLTSETGD ANGDIVGVKI ADFGLSRAQG SRQNTDYVST RWYRAPELLL HYSGYGPPID IWAAGCCFAE LLTGDPLFPG DTEIQQLQLI CAFLKPLKPD ACKGAMHALP RFITENMTVS DADIYTLAEA REALRRELGC SHVLADLLAH MLAWNPEDRI TAQRALMHQF FAI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatgcctac acgccggtgc gtcgcgtggg gtctggcacg tacggggatg tcctgcacgc agtggatacg aagggggatt tcgtcgccat caagatcatg aacagtcaca ccctctgtgt ctctgatcct ctccagctgc ccgaagtagc tacgctccgg aggcttccac cccatccaaa tatcattcgc cttattcgag tagttgtcac tagggaccac acacacttga tcttcgaata cgcaggtgga ggtaccctcc tgaattttct tcgatcttcg ggaccactca aagagcacat gctacgaaac attgcattcc gcattctcca gggcctctat cacatccata cgaacagcgt gattcaccgg gatttgaaac tagagaacat tctattaacg agcgagacgg gagatgccaa cggagacatt gttggggtaa agatagctga ctttggcctt tcaagggcac agggatcccg tcaaaatacc gattatgtta gcacgcggtg gtatagagca cctgagctcc ttctgcacta tagcggatat ggccctccaa tcgacatctg ggcagcaggt tgttgctttg ctgagctgct tacgggcgat cctctgttcc ccggcgacac cgagattcag cagctgcagc tcatctgtgc cttcctaaaa cccctaaagc cggatgcctg caaaggcgcc atgcatgcct tgcctcgctt cataaccgaa aacatgacag tgtcagatgc cgacatttat acgctagctg aagctagaga agctcttcgc agagagctcg gctgcagtca tgtgttggca gatctcctcg cacacatgct tgcgtggaat cctgaggacc gtattactgc tcagcgcgcc cttatgcacc agttcttcgc aataaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for GilaA.00542.a.A1.GE30657
HARVESTED ON: 10/27/2010
SEQUENCED ON: 11/3/2010
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDAYTPVRR VGSGTYGDVL HAVDTKGDFV AIKIMNSHTL CVSDPLQLPE VATLRRLPPH PNIIRLIRVV VTRDHTHLIF EYAGGGTLLN FLRSSGPLKE HMLRNIAFRI LQGLYHIHTN SVIHRDLKLE NILLTSETGD ANGDIVGVKI ADFGLSRAQG SRQNTDYVST RWYRAPELLL HYSGYGPPID IWAAGCCFAE LLTGDPLFPG DTEIQQLQLI CAFLKPLKPD ACKGAMHALP RFITENMTVS DADIYTLAEA REALRRELGC SHVLADLLAH MLAWNPEDRI TAQRALMHQF FAI
Validated NT Sequence
ttattatatt gcgaagaact ggtgcataag ggcgcgctga gcagtaatac ggtcctcagg attccacgca agcatgtgtg cgaggagatc tgccaacaca tgactgcagc cgagctctct gcgaagagct tctctagctt cagctagcgt ataaatgtcg gcatctgaca ctgtcatgtt ttcggttatg aagcgaggca aggcatgcat ggcgcctttg caggcatccg gctttagggg ttttaggaag gcacagatga gctgcagctg ctgaatctcg gtgtcgccgg ggaacagagg atcgcccgta agcagctcag caaagcaaca acctgctgcc cagatgtcga ttggagggcc atatccgcta tagtgcagaa ggagctcagg tgctctatac caccgcgtgc taacataatc ggtattttga cgggatccct gtgcccttga aaggccaaag tcagctatct ttaccccaac aatgtctccg ttggcatctc ccgtctcgct cgttaataga atgttctcta gtttcaaatc ccggtgaatc acgctgttcg tatggatgtg atagaggccc tggagaatgc ggaatgcaat gtttcgtagc atgtgctctt tgagtggtcc cgaagatcga agaaaattca ggagggtacc tccacctgcg tattcgaaga tcaagtgtgt gtggtcccta gtgacaacta ctcgaataag gcgaatgata tttggatggg gtggaagcct ccggagcgta gctacttcgg gcagctggag aggatcagag acacagaggg tgtgactgtt catgatcttg atggcgacga aatccccctt cgtatccact gcgtgcagga catccccgta cgtgccagac cccacgcgac gcaccggcgt gtaggcatcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDAYTPVRR VGSGTYGDVL HAVDTKGDFV AIKIMNSHTL CVSDPLQLPE VATLRRLPPH PNIIRLIRVV VTRDHTHLIF EYAGGGTLLN FLRSSGPLKE HMLRNIAFRI LQGLYHIHTN SVIHRDLKLE NILLTSETGD ANGDIVGVKI ADFGLSRAQG SRQNTDYVST RWYRAPELLL HYSGYGPPID IWAAGCCFAE LLTGDPLFPG DTEIQQLQLI CAFLKPLKPD ACKGAMHALP RFITENMTVS DADIYTLAEA REALRRELGC SHVLADLLAH MLAWNPEDRI TAQRALMHQF FAI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatgcctac acgccggtgc gtcgcgtggg gtctggcacg tacggggatg tcctgcacgc agtggatacg aagggggatt tcgtcgccat caagatcatg aacagtcaca ccctctgtgt ctctgatcct ctccagctgc ccgaagtagc tacgctccgg aggcttccac cccatccaaa tatcattcgc cttattcgag tagttgtcac tagggaccac acacacttga tcttcgaata cgcaggtgga ggtaccctcc tgaattttct tcgatcttcg ggaccactca aagagcacat gctacgaaac attgcattcc gcattctcca gggcctctat cacatccata cgaacagcgt gattcaccgg gatttgaaac tagagaacat tctattaacg agcgagacgg gagatgccaa cggagacatt gttggggtaa agatagctga ctttggcctt tcaagggcac agggatcccg tcaaaatacc gattatgtta gcacgcggtg gtatagagca cctgagctcc ttctgcacta tagcggatat ggccctccaa tcgacatctg ggcagcaggt tgttgctttg ctgagctgct tacgggcgat cctctgttcc ccggcgacac cgagattcag cagctgcagc tcatctgtgc cttcctaaaa cccctaaagc cggatgcctg caaaggcgcc atgcatgcct tgcctcgctt cataaccgaa aacatgacag tgtcagatgc cgacatttat acgctagctg aagctagaga agctcttcgc agagagctcg gctgcagtca tgtgttggca gatctcctcg cacacatgct tgcgtggaat cctgaggacc gtattactgc tcagcgcgcc cttatgcacc agttcttcgc aataaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa