GilaA.00526.a

Translation initiation factor eIF-4A, putative

CENTER ID: GilaA.00526.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.00526.a.A1.GE25647 Full length( GilaA.00526.a ) 1 391
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.00526.a.A1.PS00101 Full length( GilaA.00526.a ) 1 391

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_10255
RefSeq: XP_001709079.1
UniProt: A8B6X3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEDSTNRAEI AQTPDLKSYE KFDDMNLHPD LLFGIFTYGY KIPSAIQSQA IVPIISGKDT IAQAQSGTGK TAAFTIGMLQ RIDIGLKSPQ AIILSPTREL ALQTLKVVDG IGSRLKVQVA QCIGGTQVDD DIAAAQSCHL IVATPGRLLS LLQKKYVTTS NVKMVVLDEA DEMLSRGFTE QIVSIMKFMN ADIQIVLVSA TLPPEILELT RQFMRDPVSI LVKEAELTLD GIRQYVVELQ DAWKTEVVED IYKVLSVQQG VIFCNSIGRV KELAEKLKSA GHTLSCIHSE LDQAERNKIM GEFRSGQTRI LIATNIIARG IDVQNVSLVI NYDIPREPET YLHRIGRSGR FGRKGVAINF VTDKDKQSMQ AITDKFNVTT ENLPEDLSSL F
NT Sequence
ATGGAGGACA GCACCAACAG AGCAGAGATA GCACAGACAC CGGACTTGAA GAGCTATGAA AAGTTTGACG ACATGAACCT TCATCCGGAT CTCCTGTTCG GCATTTTTAC CTATGGCTAT AAGATACCGT CTGCTATTCA GTCCCAGGCG ATTGTTCCCA TCATCTCTGG AAAGGATACA ATCGCCCAAG CGCAGTCTGG GACGGGCAAA ACGGCAGCAT TCACAATTGG CATGCTCCAG CGAATTGACA TCGGGCTCAA GTCGCCTCAG GCCATTATTC TCTCCCCCAC TCGTGAGCTT GCGCTTCAGA CGTTGAAGGT TGTCGACGGA ATTGGGTCTC GCCTCAAGGT CCAGGTTGCC CAGTGCATTG GAGGCACACA GGTCGACGAT GACATTGCGG CTGCGCAGTC GTGCCATCTG ATTGTCGCGA CACCAGGCCG ACTTCTTTCT CTCCTACAGA AAAAATACGT GACTACCTCC AATGTGAAGA TGGTGGTCCT TGATGAGGCC GACGAGATGC TATCCAGGGG GTTCACTGAG CAGATAGTCT CGATCATGAA GTTCATGAAT GCCGACATTC AGATCGTGCT TGTCTCGGCC ACGCTTCCCC CCGAGATCCT CGAGCTGACC AGGCAATTCA TGAGGGATCC CGTGAGCATC CTCGTCAAGG AGGCTGAGCT CACCCTCGAC GGGATCAGGC AGTATGTGGT CGAGCTGCAG GACGCCTGGA AGACAGAGGT TGTCGAGGAC ATATACAAAG TTCTGAGCGT ACAGCAGGGC GTTATCTTTT GTAATTCCAT TGGGCGCGTG AAGGAGCTCG CAGAGAAGCT GAAGAGTGCT GGTCACACGC TCTCCTGTAT TCACTCCGAG CTTGACCAGG CCGAGCGCAA TAAGATTATG GGAGAGTTCC GCTCTGGCCA GACGCGCATC CTCATCGCTA CTAATATCAT TGCACGTGGT ATTGATGTTC AGAACGTCTC CCTGGTAATC AACTATGACA TACCAAGAGA GCCCGAGACG TACCTGCACC GTATCGGCAG GTCTGGGCGC TTCGGAAGAA AGGGTGTTGC TATCAATTTT GTAACAGACA AGGATAAGCA GAGCATGCAG GCAATCACTG ATAAGTTCAA CGTCACGACT GAAAACCTTC CCGAGGACCT CAGCAGCCTC TTT
Details for GilaA.00526.a.A1.GE25647
HARVESTED ON: 10/17/2008
SEQUENCED ON: 7/20/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEDSTNRAE IAQTPDLKSY EKFDDMNLHP DLLFGIFTYG YKIPSAIQSQ AIVPIISGKD TIAQAQSGTG KTAAFTIGML QRIDIGLKSP QAIILSPTRE LALQTLKVVD GIGSRLKVQV AQCIGGTQVD DDIAAAQSCH LIVATPGRLL SLLQKKYVTT SNVKMVVLDE ADEMLSRGFT EQIVSIMKFM NADIQIVLVS ATLPPEILEL TRQFMRDPVS ILVKEAELTL DGIRQYVVEL QDAWKTEVVE DIYKVLSVQQ GVIFCNSIGR VKELAEKLKS AGHTLSCIHS ELDQAERNKI MGEFRSGQTR ILIATNIIAR GIDVQNVSLV INYDIPREPE TYLHRIGRSG RFGRKGVAIN FVTDKDKQSM QAITDKFNVT TENLPEDLSS LF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggagg acagcaccaa cagagcagag atagcacaga caccggactt gaagagctat gaaaagtttg acgacatgaa ccttcatccg gatctcctgt tcggcatttt tacctatggc tataagatac cgtctgctat tcagtcccag gcgattgttc ccatcatctc tggaaaggat acaatcgccc aagcgcagtc tgggacgggc aaaacggcag cattcacaat tggcatgctc cagcgaattg acatcgggct caagtcgcct caggccatta ttctctcccc cactcgtgag cttgcgcttc agacgttgaa ggttgtcgac ggaattgggt ctcgcctcaa ggtccaggtt gcccagtgca ttggaggcac acaggtcgac gatgacattg cggctgcgca gtcgtgccat ctgattgtcg cgacaccagg ccgacttctt tctctcctac agaaaaaata cgtgactacc tccaatgtga agatggtggt ccttgatgag gccgacgaga tgctatccag ggggttcact gagcagatag tctcgatcat gaagttcatg aatgccgaca ttcagatcgt gcttgtctcg gccacgcttc cccccgagat cctcgagctg accaggcaat tcatgaggga tcccgtgagc atcctcgtca aggaggctga gctcaccctc gacgggatca ggcagtatgt ggtcgagctg caggacgcct ggaagacaga ggttgtcgag gacatataca aagttctgag cgtacagcag ggcgttatct tttgtaattc cattgggcgc gtgaaggagc tcgcagagaa gctgaagagt gctggtcaca cgctctcctg tattcactcc gagcttgacc aggccgagcg caataagatt atgggagagt tccgctctgg ccagacgcgc atcctcatcg ctactaatat cattgcacgt ggtattgatg ttcagaacgt ctccctggta atcaactatg acataccaag agagcccgag acgtacctgc accgtatcgg caggtctggg cgcttcggaa gaaagggtgt tgctatcaat tttgtaacag acaaggataa gcagagcatg caggcaatca ctgataagtt caacgtcacg actgaaaacc ttcccgagga cctcagcagc ctcttttaat aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEDSTNRAE IAQTPDLKSY EKFDDMNLHP DLLFGIFTYG YKIPSAIQSQ AIVPIISGKD TIAQAQSGTG KTAAFTIGML QRIDIGLKSP QAIILSPTRE LALQTLKVVD GIGSRLKVQV AQCIGGTQVD DDIAAAQSCH LIVATPGRLL SLLQKKYVTT SNVKMVVLDE ADEMLSRGFT EQIVSIMKFM NADIQIVLVS ATLPPEILEL TRQFMRDPVS ILVKEAELTL DGIRQYVVEL QDAWKTEVVE DIYKVLSVQQ GVIFCNSIGR VKELAEKLKS AGHTLSCIHS ELDQAERNKI MGEFRSGQTR ILIATNIIAR GIDVQNVSLV INYDIPREPE TYLHRIGRSG RFGRKGVAIN FVTDKDKQSM QAITDKFNVT TENLPEDLSS LF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaggacagc accaacagag cagagatagc acagacaccg gacttgaaga gctatgaaaa gtttgacgac atgaaccttc atccggatct cctgttcggc atttttacct atggctataa gataccgtct gctattcagt cccaggcgat tgttcccatc atctctggaa aggatacaat cgcccaagcg cagtctggga cgggcaaaac ggcagcattc acaattggca tgctccagcg aattgacatc gggctcaagt cgcctcaggc cattattctc tcccccactc gtgagcttgc gcttcagacg ttgaaggttg tcgacggaat tgggtctcgc ctcaaggtcc aggttgccca gtgcattgga ggcacacagg tcgacgatga cattgcggct gcgcagtcgt gccatctgat tgtcgcgaca ccaggccgac ttctttctct cctacagaaa aaatacgtga ctacctccaa tgtgaagatg gtggtccttg atgaggccga cgagatgcta tccagggggt tcactgagca gatagtctcg atcatgaagt tcatgaatgc cgacattcag atcgtgcttg tctcggccac gcttcccccc gagatcctcg agctgaccag gcaattcatg agggatcccg tgagcatcct cgtcaaggag gctgagctca ccctcgacgg gatcaggcag tatgtggtcg agctgcagga cgcctggaag acagaggttg tcgaggacat atacaaagtt ctgagcgtac agcagggcgt tatcttttgt aattccattg ggcgcgtgaa ggagctcgca gagaagctga agagtgctgg tcacacgctc tcctgtattc actccgagct tgaccaggcc gagcgcaata agattatggg agagttccgc tctggccaga cgcgcatcct catcgctact aatatcattg cacgtggtat tgatgttcag aacgtctccc tggtaatcaa ctatgacata ccaagagagc ccgagacgta cctgcaccgt atcggcaggt ctgggcgctt cggaagaaag ggtgttgcta tcaattttgt aacagacaag gataagcaga gcatgcaggc aatcactgat aagttcaacg tcacgactga aaaccttccc gaggacctca gcagcctctt taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for GilaA.00526.a.A1.PS00101
PURIFICATION DATe: 11/18/2008
CONCENTRATION: 51.88mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEDSTNRAE IAQTPDLKSY EKFDDMNLHP DLLFGIFTYG YKIPSAIQSQ AIVPIISGKD TIAQAQSGTG KTAAFTIGML QRIDIGLKSP QAIILSPTRE LALQTLKVVD GIGSRLKVQV AQCIGGTQVD DDIAAAQSCH LIVATPGRLL SLLQKKYVTT SNVKMVVLDE ADEMLSRGFT EQIVSIMKFM NADIQIVLVS ATLPPEILEL TRQFMRDPVS ILVKEAELTL DGIRQYVVEL QDAWKTEVVE DIYKVLSVQQ GVIFCNSIGR VKELAEKLKS AGHTLSCIHS ELDQAERNKI MGEFRSGQTR ILIATNIIAR GIDVQNVSLV INYDIPREPE TYLHRIGRSG RFGRKGVAIN FVTDKDKQSM QAITDKFNVT TENLPEDLSS LF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggagg acagcaccaa cagagcagag atagcacaga caccggactt gaagagctat gaaaagtttg acgacatgaa ccttcatccg gatctcctgt tcggcatttt tacctatggc tataagatac cgtctgctat tcagtcccag gcgattgttc ccatcatctc tggaaaggat acaatcgccc aagcgcagtc tgggacgggc aaaacggcag cattcacaat tggcatgctc cagcgaattg acatcgggct caagtcgcct caggccatta ttctctcccc cactcgtgag cttgcgcttc agacgttgaa ggttgtcgac ggaattgggt ctcgcctcaa ggtccaggtt gcccagtgca ttggaggcac acaggtcgac gatgacattg cggctgcgca gtcgtgccat ctgattgtcg cgacaccagg ccgacttctt tctctcctac agaaaaaata cgtgactacc tccaatgtga agatggtggt ccttgatgag gccgacgaga tgctatccag ggggttcact gagcagatag tctcgatcat gaagttcatg aatgccgaca ttcagatcgt gcttgtctcg gccacgcttc cccccgagat cctcgagctg accaggcaat tcatgaggga tcccgtgagc atcctcgtca aggaggctga gctcaccctc gacgggatca ggcagtatgt ggtcgagctg caggacgcct ggaagacaga ggttgtcgag gacatataca aagttctgag cgtacagcag ggcgttatct tttgtaattc cattgggcgc gtgaaggagc tcgcagagaa gctgaagagt gctggtcaca cgctctcctg tattcactcc gagcttgacc aggccgagcg caataagatt atgggagagt tccgctctgg ccagacgcgc atcctcatcg ctactaatat cattgcacgt ggtattgatg ttcagaacgt ctccctggta atcaactatg acataccaag agagcccgag acgtacctgc accgtatcgg caggtctggg cgcttcggaa gaaagggtgt tgctatcaat tttgtaacag acaaggataa gcagagcatg caggcaatca ctgataagtt caacgtcacg actgaaaacc ttcccgagga cctcagcagc ctcttttaat aa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEDSTNRAE IAQTPDLKSY EKFDDMNLHP DLLFGIFTYG YKIPSAIQSQ AIVPIISGKD TIAQAQSGTG KTAAFTIGML QRIDIGLKSP QAIILSPTRE LALQTLKVVD GIGSRLKVQV AQCIGGTQVD DDIAAAQSCH LIVATPGRLL SLLQKKYVTT SNVKMVVLDE ADEMLSRGFT EQIVSIMKFM NADIQIVLVS ATLPPEILEL TRQFMRDPVS ILVKEAELTL DGIRQYVVEL QDAWKTEVVE DIYKVLSVQQ GVIFCNSIGR VKELAEKLKS AGHTLSCIHS ELDQAERNKI MGEFRSGQTR ILIATNIIAR GIDVQNVSLV INYDIPREPE TYLHRIGRSG RFGRKGVAIN FVTDKDKQSM QAITDKFNVT TENLPEDLSS LF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAGGACAGC ACCAACAGAG CAGAGATAGC ACAGACACCG GACTTGAAGA GCTATGAAAA GTTTGACGAC ATGAACCTTC ATCCGGATCT CCTGTTCGGC ATTTTTACCT ATGGCTATAA GATACCGTCT GCTATTCAGT CCCAGGCGAT TGTTCCCATC ATCTCTGGAA AGGATACAAT CGCCCAAGCG CAGTCTGGGA CGGGCAAAAC GGCAGCATTC ACAATTGGCA TGCTCCAGCG AATTGACATC GGGCTCAAGT CGCCTCAGGC CATTATTCTC TCCCCCACTC GTGAGCTTGC GCTTCAGACG TTGAAGGTTG TCGACGGAAT TGGGTCTCGC CTCAAGGTCC AGGTTGCCCA GTGCATTGGA GGCACACAGG TCGACGATGA CATTGCGGCT GCGCAGTCGT GCCATCTGAT TGTCGCGACA CCAGGCCGAC TTCTTTCTCT CCTACAGAAA AAATACGTGA CTACCTCCAA TGTGAAGATG GTGGTCCTTG ATGAGGCCGA CGAGATGCTA TCCAGGGGGT TCACTGAGCA GATAGTCTCG ATCATGAAGT TCATGAATGC CGACATTCAG ATCGTGCTTG TCTCGGCCAC GCTTCCCCCC GAGATCCTCG AGCTGACCAG GCAATTCATG AGGGATCCCG TGAGCATCCT CGTCAAGGAG GCTGAGCTCA CCCTCGACGG GATCAGGCAG TATGTGGTCG AGCTGCAGGA CGCCTGGAAG ACAGAGGTTG TCGAGGACAT ATACAAAGTT CTGAGCGTAC AGCAGGGCGT TATCTTTTGT AATTCCATTG GGCGCGTGAA GGAGCTCGCA GAGAAGCTGA AGAGTGCTGG TCACACGCTC TCCTGTATTC ACTCCGAGCT TGACCAGGCC GAGCGCAATA AGATTATGGG AGAGTTCCGC TCTGGCCAGA CGCGCATCCT CATCGCTACT AATATCATTG CACGTGGTAT TGATGTTCAG AACGTCTCCC TGGTAATCAA CTATGACATA CCAAGAGAGC CCGAGACGTA CCTGCACCGT ATCGGCAGGT CTGGGCGCTT CGGAAGAAAG GGTGTTGCTA TCAATTTTGT AACAGACAAG GATAAGCAGA GCATGCAGGC AATCACTGAT AAGTTCAACG TCACGACTGA AAACCTTCCC GAGGACCTCA GCAGCCTCTT TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA