SSGCID: Target Page

GilaA.00525.a

Kinase, CMGC CDK

CENTER ID:	GilaA.00525.a
ORGANISM:	Giardia lamblia ATCC 50803
ASSOCIATED DISEASE:	Giardiasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
GilaA.00525.a.A1.PW27695	Full length( GilaA.00525.a )		1	291

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	GiardiaDB:GL50803_16802
RefSeq:	XP_001709931.1
UniProt:	A8B2A9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDPLNRYER IQGLGEGTYG VVFKAKDKET GQIVALKRIR LENADEGIPA TAIREIAILK EMKHKNVVDL LSVIHTEAKL TLVFEYLDMD LKKYIDSKQG KLTPKEVKSF MGQLMTGLTY IHNKRVLHRD LKPQNLLVTS SGLLKLADFG LARGSGIPVR SYTHEVVTLW YRCPSVLLGC RKYGGALDIW SCGCIFYECV TGKPLFPAKT EKDELIKIFK TLGTPDKQSW PDVDTLPQWQ KDFPVYPGIN VAELLPTLDE AGRDLFSKMM ALDPSKRPSA RDCLKHPYFA K

NT Sequence

ATGACTGACC CCTTGAACAG ATATGAAAGA ATACAGGGCC TAGGAGAAGG AACCTACGGC GTCGTCTTCA AGGCAAAGGA CAAAGAGACA GGTCAGATTG TCGCCCTCAA GAGGATTAGG CTCGAGAATG CGGACGAGGG TATCCCGGCC ACTGCAATCC GTGAGATCGC TATACTCAAA GAGATGAAGC ACAAGAACGT TGTAGACCTT CTCTCCGTCA TTCACACAGA AGCGAAACTC ACGCTTGTAT TTGAGTACCT CGACATGGAC CTGAAGAAGT ACATCGACTC CAAGCAAGGA AAGCTAACAC CTAAGGAGGT CAAGAGCTTT ATGGGGCAGC TGATGACTGG GCTCACGTAT ATACACAACA AGCGCGTCTT GCACCGTGAT CTAAAGCCAC AGAATCTGCT AGTTACCAGC TCTGGGCTCC TTAAGCTCGC AGACTTTGGC CTGGCGCGTG GATCTGGAAT CCCCGTTCGA TCATATACTC ATGAGGTGGT CACGCTTTGG TACCGTTGCC CATCGGTTCT TCTGGGCTGC CGCAAGTACG GTGGAGCTCT CGATATATGG TCTTGTGGGT GCATCTTCTA TGAGTGCGTC ACTGGGAAGC CGCTGTTCCC AGCCAAGACA GAAAAGGATG AACTTATTAA GATCTTCAAG ACCCTTGGAA CTCCAGACAA ACAGAGCTGG CCGGATGTCG ATACGCTTCC CCAATGGCAA AAGGACTTCC CTGTCTACCC AGGCATAAAC GTTGCCGAGC TTCTGCCGAC TCTTGACGAG GCCGGCAGAG ACCTCTTTTC AAAGATGATG GCTCTTGACC CTTCCAAACG CCCTTCGGCA AGAGACTGCC TCAAGCATCC GTACTTTGCA AAG

Details for GilaA.00525.a.A1.PW27695

PURIFICATION DATe:	10/26/2009
CONCENTRATION:	22.03mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTDPLNRYE RIQGLGEGTY GVVFKAKDKE TGQIVALKRI RLENADEGIP ATAIREIAIL KEMKHKNVVD LLSVIHTEAK LTLVFEYLDM DLKKYIDSKQ GKLTPKEVKS FMGQLMTGLT YIHNKRVLHR DLKPQNLLVT SSGLLKLADF GLARGSGIPV RSYTHEVVTL WYRCPSVLLG CRKYGGALDI WSCGCIFYEC VTGKPLFPAK TEKDELIKIF KTLGTPDKQS WPDVDTLPQW QKDFPVYPGI NVAELLPTLD EAGRDLFSKM MALDPSKRPS ARDCLKHPYF AK

Validated NT Sequence

ttattacttt gcaaagtacg gatgcttgag gcagtctctt gccgaagggc gtttggaagg gtcaagagcc atcatctttg aaaagaggtc tctgccggcc tcgtcaagag tcggcagaag ctcggcaacg tttatgcctg ggtagacagg gaagtccttt tgccattggg gaagcgtatc gacatccggc cagctctgtt tgtctggagt tccaagggtc ttgaagatct taataagttc atccttttct gtcttggctg ggaacagcgg cttcccagtg acgcactcat agaagatgca cccacaagac catatatcga gagctccacc gtacttgcgg cagcccagaa gaaccgatgg gcaacggtac caaagcgtga ccacctcatg agtatatgat cgaacgggga ttccagatcc acgcgccagg ccaaagtctg cgagcttaag gagcccagag ctggtaacta gcagattctg tggctttaga tcacggtgca agacgcgctt gttgtgtata tacgtgagcc cagtcatcag ctgccccata aagctcttga cctccttagg tgttagcttt ccttgcttgg agtcgatgta cttcttcagg tccatgtcga ggtactcaaa tacaagcgtg agtttcgctt ctgtgtgaat gacggagaga aggtctacaa cgttcttgtg cttcatctct ttgagtatag cgatctcacg gattgcagtg gccgggatac cctcgtccgc attctcgagc ctaatcctct tgagggcgac aatctgacct gtctctttgt cctttgcctt gaagacgacg ccgtaggttc cttctcctag gccctgtatt ctttcatatc tgttcaaggg gtcagtcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACTGACCCC TTGAACAGAT ATGAAAGAAT ACAGGGCCTA GGAGAAGGAA CCTACGGCGT CGTCTTCAAG GCAAAGGACA AAGAGACAGG TCAGATTGTC GCCCTCAAGA GGATTAGGCT CGAGAATGCG GACGAGGGTA TCCCGGCCAC TGCAATCCGT GAGATCGCTA TACTCAAAGA GATGAAGCAC AAGAACGTTG TAGACCTTCT CTCCGTCATT CACACAGAAG CGAAACTCAC GCTTGTATTT GAGTACCTCG ACATGGACCT GAAGAAGTAC ATCGACTCCA AGCAAGGAAA GCTAACACCT AAGGAGGTCA AGAGCTTTAT GGGGCAGCTG ATGACTGGGC TCACGTATAT ACACAACAAG CGCGTCTTGC ACCGTGATCT AAAGCCACAG AATCTGCTAG TTACCAGCTC TGGGCTCCTT AAGCTCGCAG ACTTTGGCCT GGCGCGTGGA TCTGGAATCC CCGTTCGATC ATATACTCAT GAGGTGGTCA CGCTTTGGTA CCGTTGCCCA TCGGTTCTTC TGGGCTGCCG CAAGTACGGT GGAGCTCTCG ATATATGGTC TTGTGGGTGC ATCTTCTATG AGTGCGTCAC TGGGAAGCCG CTGTTCCCAG CCAAGACAGA AAAGGATGAA CTTATTAAGA TCTTCAAGAC CCTTGGAACT CCAGACAAAC AGAGCTGGCC GGATGTCGAT ACGCTTCCCC AATGGCAAAA GGACTTCCCT GTCTACCCAG GCATAAACGT TGCCGAGCTT CTGCCGACTC TTGACGAGGC CGGCAGAGAC CTCTTTTCAA AGATGATGGC TCTTGACCCT TCCAAACGCC CTTCGGCAAG AGACTGCCTC AAGCATCCGT ACTTTGCAAA GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA