GilaA.00438.a
Nucleoside diphosphate kinase
| CENTER ID: | GilaA.00438.a |
| ORGANISM: | Giardia lamblia ATCC 50803 |
| ASSOCIATED DISEASE: | Giardiasis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| GilaA.00438.a.A1.GE25640 | Full length( GilaA.00438.a ) | 1 | 151 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3R9L
DEPOSITED: 3/25/2011
DETERMINATION: XRay
CLONE: GilaA.00438.a.A1.GE25640
PROTEIN: GilaA.00438.a.A1.PW27083
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | GiardiaDB:GL50803_11301 |
| OrthoMCL: | OG5_126708 |
| RefSeq: | XP_001706095.1 |
| UniProt: | A8BMG9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARERTFLMV KPDGVQRGLV GEIISRFERR GFKLVAMKFF VPSKNLVEEH YKEHAARPFF AGLCKFLSSG PVCAMVWEGA NVVSISRTMM GVTKPAESAP GTIRGDFGID VGRNIIHGSA NLDDAAREIA LWFKPEEVAS WSCSLESHIY E
NT Sequence
ATGGCACGTG AACGCACCTT TCTCATGGTG AAGCCGGACG GTGTCCAGCG CGGCCTCGTC GGCGAGATAA TCTCCCGCTT CGAGCGCAGG GGCTTCAAGC TGGTCGCCAT GAAGTTCTTT GTGCCTTCGA AGAACCTTGT CGAGGAGCAC TACAAGGAGC ACGCCGCCAG GCCTTTCTTC GCCGGGCTCT GCAAGTTCTT GTCCAGCGGC CCCGTCTGCG CCATGGTCTG GGAGGGCGCC AATGTGGTGA GCATATCCCG CACCATGATG GGGGTCACGA AGCCGGCCGA GTCTGCTCCC GGCACGATAC GCGGGGACTT CGGCATTGAC GTCGGGAGGA ACATCATTCA CGGCTCTGCC AATCTCGATG ATGCGGCGCG TGAGATAGCT CTCTGGTTCA AGCCAGAGGA GGTGGCCTCG TGGAGCTGCT CTCTCGAGAG CCACATCTAT GAG
Details for GilaA.00438.a.A1.GE25640
| HARVESTED ON: | 10/17/2008 |
| SEQUENCED ON: | 7/20/2010 |
| EXPECTED MW: | 19kDa |
| OBSERVED MW: | 20kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMARERTFLM VKPDGVQRGL VGEIISRFER RGFKLVAMKF FVPSKNLVEE HYKEHAARPF FAGLCKFLSS GPVCAMVWEG ANVVSISRTM MGVTKPAESA PGTIRGDFGI DVGRNIIHGS ANLDDAAREI ALWFKPEEVA SWSCSLESHI
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcac gtgaacgcac ctttctcatg gtgaagccgg acggtgtcca gcgcggcctc gtcggcgaga taatctcccg cttcgagcgc aggggcttca agctggtcgc catgaagttc tttgtgcctt cgaagaacct tgtcgaggag cactacaagg agcacgccgc caggcctttc ttcgccgggc tctgcaagtt cttgtccagc ggccccgtct gcgccatggt ctgggagggc gccaatgtgg tgagcatatc ccgcaccatg atgggggtca cgaagccggc cgagtctgct cccggcacga tacgcgggga cttcggcatt gacgtcggga ggaacatcat tcacggctct gccaatctcg atgatgcggc gcgtgagata gctctctggt tcaagccaga ggaggtggcc tcgtggagct gctctctcga gagccacatc t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARERTFLM VKPDGVQRGL VGEIISRFER RGFKLVAMKF FVPSKNLVEE HYKEHAARPF FAGLCKFLSS GPVCAMVWEG ANVVSISRTM MGVTKPAESA PGTIRGDFGI DVGRNIIHGS ANLDDAAREI ALWFKPEEVA SWSCSLESHI YE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcacgtgaa cgcacctttc tcatggtgaa gccggacggt gtccagcgcg gcctcgtcgg cgagataatc tcccgcttcg agcgcagggg cttcaagctg gtcgccatga agttctttgt gccttcgaag aaccttgtcg aggagcacta caaggagcac gccgccaggc ctttcttcgc cgggctctgc aagttcttgt ccagcggccc cgtctgcgcc atggtctggg agggcgccaa tgtggtgagc atatcccgca ccatgatggg ggtcacgaag ccggccgagt ctgctcccgg cacgatacgc ggggacttcg gcattgacgt cgggaggaac atcattcacg gctctgccaa tctcgatgat gcggcgcgtg agatagctct ctggttcaag ccagaggagg tggcctcgtg gagctgctct ctcgagagcc acatctatga gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa