GilaA.00424.b

Kinase, NEK

CENTER ID: GilaA.00424.b
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.00424.b.B1.GE37524 full length 1 584
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_9421
RefSeq: XP_001707788.1
UniProt: A8BDH0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAHLFSQTGY NTLALIGQGA YGKCYKVQHQ ATGQIFAVKV MTYTNPEGKT RQLLVQEMKI HRMLAHPNIV RCVSATHDED AMEIRMFSEF CSGGDLHAYV QKLEKGEYIP EDRLWSIMGQ ILCALKYCHS PAKPGFAVGK KVVHRDLKPA NIMLTDEGVI KLCDFGFARE IDFDNSAMTL VGSPMYMAPE VFQGKTYDEK CDIWSLGGVM HFLASKLVPY LAKTRPELEK LVLAERRAPL PKHYSKEFVD TIGMMMRLNP EDRPSAADLL DLPRFANMFP DQDVNEVTNL DNLETGDATE EPAKAVVTKK PMNPSELAAK ITETTKNIQP AAPLSPMSAN KASYEAQIDA LNKQLDQQNE LLLEAEKNIG SVTELAKGRL EMIASLRYSK NGRAVTDKGE TALILAVKVR DYELIKRLLP LDAGIGNNLG KTALMYAADM DDATSIRLLL SVEGGMQDNP GGTALMRACN GGKVTAASML LMHEKGLAKK DGSTALIFAI MSGSDTLVDL LVPYEAGMAM KDGRTALMSA CFAGRFYAVK RLVEKEFHMK DSNGKTCIYY AERGGHKAVI DFVKEWAEKH PEAD
NT Sequence
atggcacacc tatttagcca aacaggctat aacacgctgg cccttatagg gcagggcgct tatggaaagt gttacaaggt ccagcatcag gctacggggc aaattttcgc cgttaaagtg atgacctata cgaatcccga ggggaagaca cgccagctct tggttcagga gatgaaaata caccgcatgc ttgctcaccc aaatatagtt cgctgtgttt ctgcaacgca tgacgaagat gctatggaga tacgcatgtt ctccgagttc tgctccgggg gagacttgca tgcatatgtc cagaagttag agaagggcga atatattccg gaggatagac tctggagtat catgggccaa attctatgtg ccctcaagta ctgccactcg cctgcaaagc cgggtttcgc tgtcggcaaa aaggtcgtgc atagggacct caagcctgca aacattatgt taacagatga aggtgttata aaactctgcg atttcggctt tgcacgtgaa attgattttg ataactcagc aatgaccctt gttggatctc cgatgtacat ggcgccagag gtcttccagg gaaaaacgta tgatgagaaa tgtgatattt ggagtctggg gggtgttatg cactttttgg cgtcaaagct agttccgtat cttgctaaga cgcgtccgga gctagaaaag ctagtgcttg cagaacgtag agcaccttta cctaagcatt atagcaaaga attcgtagat acaataggca tgatgatgcg tctaaatcct gaagacaggc catccgctgc agatttactc gatctgccca gatttgccaa catgttcccc gatcaggatg ttaatgaggt cactaatctg gacaatctgg aaactggcga tgcaacggag gagcctgcga aagcggtggt gactaagaag ccgatgaacc ccagtgaact tgcagcaaag attacagaga caaccaagaa catacagcct gctgctccct taagtcccat gagtgctaac aaagcgtcgt acgaagctca gattgacgcc cttaacaagc aactggatca gcagaacgag ctcttgcttg aagcggagaa gaatattggc tcagttactg agcttgctaa agggcgtctc gagatgatag cctctcttcg ttattccaaa aatggtcgtg cggtcacaga caagggagaa acggcactta ttttggccgt gaaagttcgt gattacgagc tgatcaaaag acttcttccc cttgacgctg gaatcggtaa taatttgggt aaaacggcct taatgtacgc agctgacatg gatgatgcca cgtctatcag gcttcttctt tcagtcgagg gcgggatgca ggacaatccc ggaggcacgg cgcttatgag agcctgtaac ggtgggaaag tcactgctgc gtccatgctt cttatgcacg aaaagggctt ggccaaaaag gacgggtcca ctgcccttat cttcgcgatt atgagcggct ctgatactct ggtagacctt ttggttcctt acgaagcggg aatggcaatg aaggacggga gaaccgccct catgagcgct tgttttgcag gccgcttcta tgcagtcaag aggctcgtcg aaaaagagtt ccacatgaag gactcaaacg gaaaaacttg catttactac gccgaaagag gcggacataa agcggtgata gactttgtga aggagtgggc agagaagcat ccggaagcag actag
Details for GilaA.00424.b.B1.GE37524
HARVESTED ON: 11/25/2013
SEQUENCED ON: 12/6/2013
EXPECTED MW: 65kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA HLFSQTGYNT LALIGQGAYG KCYKVQHQAT GQIFAVKVMT YTNPEGKTRQ LLVQEMKIHR MLAHPNIVRC VSATHDEDAM EIRMFSEFCS GGDLHAYVQK LEKGEYIPED RLWSIMGQIL CALKYCHSPA KPGFAVGKKV VHRDLKPANI MLTDEGVIKL CDFGFAREID FDNSAMTLVG SPMYMAPEVF QGKTYDEKCD IWSLGGVMHF LASKLVPYLA KTRPELEKLV LAERRAPLPK HYSKEFVDTI GMMMRLNPED RPSAADLLDL PRFANMFPDQ DVNEVTNLDN LETGDATEEP AKAVVTKKPM NPSELAAKIT ETTKNIQPAA PLSPMSANKA SYEAQIDALN KQLDQQNELL LEAEKNIGSV TELAKGRLEM IASLRYSKNG RAVTDKGETA LILAVKVRDY ELIKRLLPLD AGIGNNLGKT ALMYAADMDD ATSIRLLLSV EGGMQDNPGG TALMRACNGG KVTAASMLLM HEKGLAKKDG STALIFAIMG GSDTLVDLLV PYEAGMAXKD GRTALMSACF AGRFYAVKRL VEKEFHMKDS NGKTCIYYAE RGGHKAVIDF VKE
Validated NT Sequence
atggctcacc accaccacca ccatatggca cacctattta gccaaacagg ctataacacg ctggccctta tagggcaggg cgcttatgga aagtgttaca aggtccagca tcaggctacg gggcaaattt tcgccgttaa agtgatgacc tatacgaatc ccgaggggaa gacacgccag ctcttggttc aggagatgaa aatacaccgc atgcttgctc acccaaatat agttcgctgt gtttctgcaa cgcatgacga agatgctatg gagatacgca tgttctccga gttctgctcc gggggagact tgcatgcata tgtccagaag ttagagaagg gcgaatatat tccggaggat agactctgga gtatcatggg ccaaattcta tgtgccctca agtactgcca ctcgcctgca aagccgggtt tcgctgtcgg caaaaaggtc gtgcataggg acctcaagcc tgcaaacatt atgttaacag atgaaggtgt tataaaactc tgcgatttcg gctttgcacg tgaaattgat tttgataact cagcaatgac ccttgttgga tctccgatgt acatggcgcc agaggtcttc cagggaaaaa cgtatgatga gaaatgtgat atttggagtc tggggggtgt tatgcacttt ttggcgtcaa agctagttcc gtatcttgct aagacgcgtc cggagctaga aaagctagtg cttgcagaac gtagagcacc tttacctaag cattatagca aagaattcgt agatacaata ggcatgatga tgcgtctaaa tcctgaagac aggccatccg ctgcagattt actcgatctg cccagatttg ccaacatgtt ccccgatcag gatgttaatg aggtcactaa tctggacaat ctggaaactg gcgatgcaac ggaggagcct gcgaaagcgg tggtgactaa gaagccgatg aaccccagtg aacttgcagc aaagattaca gagacaacca agaacataca gcctgctgct cccttaagtc ccatgagtgc taacaaagcg tcgtacgaag ctcagattga cgcccttaac aagcaactgg atcagcagaa cgagctcttg cttgaagcgg agaagaatat tggctcagtt actgagcttg ctaaagggcg tctcgagatg atagcctctc ttcgttattc caaaaatggt cgtgcggtca cagacaaggg agaaacggca cttattttgg ccgtgaaagt tcgtgattac gagctgatca aaagacttct tccccttgac gctggaatcg gtaataattt gggtaaaacg gccttaatgt acgcagctga catggatgat gccacgtcta tcaggcttct tctttcagtc gagggcggga tgcaggacaa tcccggaggc acggcgctta tgagagcctg taacggtggg aaagtcactg ctgcgtccat gcttcttatg cacgaaaagg gcttggccaa aaaggacggg tccactgccc ttatcttcgc gattatgggc ggctctgata ctctggtaga ccttttggtt ccttacgaag cgggaatggc aangaaggac gggagaaccg ccctcatgag cgcttgtttt gcaggccgct tctatgcagt caagaggctc gtcgaaaaag agttccacat gaaggactca aacggaaaaa cttgcattta ctacgccgaa agaggcggac ataaagcggt gatagacttt gtgaaggagt gg
Expected Protein Sequence
MAHHHHHHMA HLFSQTGYNT LALIGQGAYG KCYKVQHQAT GQIFAVKVMT YTNPEGKTRQ LLVQEMKIHR MLAHPNIVRC VSATHDEDAM EIRMFSEFCS GGDLHAYVQK LEKGEYIPED RLWSIMGQIL CALKYCHSPA KPGFAVGKKV VHRDLKPANI MLTDEGVIKL CDFGFAREID FDNSAMTLVG SPMYMAPEVF QGKTYDEKCD IWSLGGVMHF LASKLVPYLA KTRPELEKLV LAERRAPLPK HYSKEFVDTI GMMMRLNPED RPSAADLLDL PRFANMFPDQ DVNEVTNLDN LETGDATEEP AKAVVTKKPM NPSELAAKIT ETTKNIQPAA PLSPMSANKA SYEAQIDALN KQLDQQNELL LEAEKNIGSV TELAKGRLEM IASLRYSKNG RAVTDKGETA LILAVKVRDY ELIKRLLPLD AGIGNNLGKT ALMYAADMDD ATSIRLLLSV EGGMQDNPGG TALMRACNGG KVTAASMLLM HEKGLAKKDG STALIFAIMS GSDTLVDLLV PYEAGMAMKD GRTALMSACF AGRFYAVKRL VEKEFHMKDS NGKTCIYYAE RGGHKAVIDF VKEWAEKHPE AD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcac acctatttag ccaaacaggc tataacacgc tggcccttat agggcagggc gcttatggaa agtgttacaa ggtccagcat caggctacgg ggcaaatttt cgccgttaaa gtgatgacct atacgaatcc cgaggggaag acacgccagc tcttggttca ggagatgaaa atacaccgca tgcttgctca cccaaatata gttcgctgtg tttctgcaac gcatgacgaa gatgctatgg agatacgcat gttctccgag ttctgctccg ggggagactt gcatgcatat gtccagaagt tagagaaggg cgaatatatt ccggaggata gactctggag tatcatgggc caaattctat gtgccctcaa gtactgccac tcgcctgcaa agccgggttt cgctgtcggc aaaaaggtcg tgcataggga cctcaagcct gcaaacatta tgttaacaga tgaaggtgtt ataaaactct gcgatttcgg ctttgcacgt gaaattgatt ttgataactc agcaatgacc cttgttggat ctccgatgta catggcgcca gaggtcttcc agggaaaaac gtatgatgag aaatgtgata tttggagtct ggggggtgtt atgcactttt tggcgtcaaa gctagttccg tatcttgcta agacgcgtcc ggagctagaa aagctagtgc ttgcagaacg tagagcacct ttacctaagc attatagcaa agaattcgta gatacaatag gcatgatgat gcgtctaaat cctgaagaca ggccatccgc tgcagattta ctcgatctgc ccagatttgc caacatgttc cccgatcagg atgttaatga ggtcactaat ctggacaatc tggaaactgg cgatgcaacg gaggagcctg cgaaagcggt ggtgactaag aagccgatga accccagtga acttgcagca aagattacag agacaaccaa gaacatacag cctgctgctc ccttaagtcc catgagtgct aacaaagcgt cgtacgaagc tcagattgac gcccttaaca agcaactgga tcagcagaac gagctcttgc ttgaagcgga gaagaatatt ggctcagtta ctgagcttgc taaagggcgt ctcgagatga tagcctctct tcgttattcc aaaaatggtc gtgcggtcac agacaaggga gaaacggcac ttattttggc cgtgaaagtt cgtgattacg agctgatcaa aagacttctt ccccttgacg ctggaatcgg taataatttg ggtaaaacgg ccttaatgta cgcagctgac atggatgatg ccacgtctat caggcttctt ctttcagtcg agggcgggat gcaggacaat cccggaggca cggcgcttat gagagcctgt aacggtggga aagtcactgc tgcgtccatg cttcttatgc acgaaaaggg cttggccaaa aaggacgggt ccactgccct tatcttcgcg attatgagcg gctctgatac tctggtagac cttttggttc cttacgaagc gggaatggca atgaaggacg ggagaaccgc cctcatgagc gcttgttttg caggccgctt ctatgcagtc aagaggctcg tcgaaaaaga gttccacatg aaggactcaa acggaaaaac ttgcatttac tacgccgaaa gaggcggaca taaagcggtg atagactttg tgaaggagtg ggcagagaag catccggaag cagactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat