GilaA.00361.a

14-3-3 protein

CENTER ID: GilaA.00361.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.00361.a.A1.GE25632 Full length( GilaA.00361.a ) 1 248
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.00361.a.A1.PW25680 Full length( GilaA.00361.a ) 1 248
External Resources
RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_6430
OrthoMCL: OG5_126706
RefSeq: XP_001706755.1
UniProt: Q2QBT8
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAEAFTREDY VFMAQLNENA ERYDEMVETM RKISGMEGEL SDKERNLLSV AYKNVIGPRR AAWRIVSSIE AKEKGRQKPN AKRIEQIRVY RQKIEKELSD ICNDILKLLQ EQFVPRSTNA DAKVFYYKMQ GDYYRYLAEY SSGEDKEKIA GSALNAYNSA FEISQQLPPT HPIRLGLALN FSVFYYEILA SPDRACELAR KAFDAAITDL DKLTEESYKD STLIMQLLRD NLNLWVTDSA GDDNAEEK
NT Sequence
ATGGCCGAGG CATTTACGCG TGAAGACTAC GTCTTTATGG CTCAGCTCAA CGAGAACGCC GAGCGCTATG ATGAGATGGT GGAGACCATG AGGAAGATCT CCGGTATGGA AGGCGAGCTC TCGGATAAGG AGCGAAACCT GCTTTCTGTA GCCTACAAGA ACGTCATCGG CCCCCGCCGC GCAGCCTGGA GGATCGTCTC CTCCATCGAG GCGAAGGAGA AGGGCCGCCA GAAGCCGAAC GCCAAGCGAA TCGAGCAGAT CCGTGTGTAC AGGCAGAAGA TAGAGAAAGA GCTTTCGGAC ATCTGCAACG ACATCCTCAA GCTCCTCCAG GAGCAGTTCG TCCCCCGCTC CACAAATGCC GACGCGAAGG TCTTCTACTA CAAGATGCAG GGAGACTACT ATAGGTACCT CGCCGAGTAC TCGTCGGGCG AGGACAAGGA GAAGATCGCC GGCTCTGCCC TCAACGCCTA CAACTCTGCG TTCGAGATCT CCCAGCAGCT GCCTCCGACC CACCCGATCC GTCTCGGGCT GGCTCTGAAC TTCTCTGTCT TCTACTATGA GATCCTCGCG TCTCCTGACA GGGCTTGCGA GCTTGCGCGC AAGGCTTTCG ACGCGGCCAT CACAGATCTG GACAAGCTGA CCGAGGAGTC TTACAAGGAC TCGACCCTCA TCATGCAGCT CCTCCGCGAC AACCTCAACC TCTGGGTGAC GGACTCTGCC GGTGACGATA ATGCCGAGGA GAAG
Details for GilaA.00361.a.A1.GE25632
HARVESTED ON: 10/17/2008
SEQUENCED ON: 7/20/2010
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAEAFTRED YVFMAQLNEN AERYDEMVET MRKISGMEGE LSDKERNLLS VAYKNVIGPR RAAWRIVSSI EAKEKGRQKP NAKRIEQIRV YRQKIEKELS DICNDILKLL QEQFVPRSTN ADAKVFYYKM QGDYYRYLAE YSSGEDKEKI AGSALNAYNS AFEISQQLPP THPIRLGLAL NFSVFYYEIL ASPDRACELA RKAFDAAITD LDKLTEESYK DSTLIMQLLR DNLNLWVTDS AGDDNAE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggccg aggcatttac gcgtgaagac tacgtcttta tggctcagct caacgagaac gccgagcgct atgatgagat ggtggagacc atgaggaaga tctccggtat ggaaggcgag ctctcggata aggagcgaaa cctgctttct gtagcctaca agaacgtcat cggcccccgc cgcgcagcct ggaggatcgt ctcctccatc gaggcgaagg agaagggccg ccagaagccg aacgccaagc gaatcgagca gatccgtgtg tacaggcaga agatagagaa agagctttcg gacatctgca acgacatcct caagctcctc caggagcagt tcgtcccccg ctccacaaat gccgacgcga aggtcttcta ctacaagatg cagggagact actataggta cctcgccgag tactcgtcgg gcgaggacaa ggagaagatc gccggctctg ccctcaacgc ctacaactct gcgttcgaga tctcccagca gctgcctccg acccacccga tccgtctcgg gctggctctg aacttctctg tcttctacta tgagatcctc gcgtctcctg acagggcttg cgagcttgcg cgcaaggctt tcgacgcggc catcacagat ctggacaagc tgaccgagga gtcttacaag gactcgaccc tcatcatgca gctcctccgc gacaacctca acctctgggt gacggactct gccggtgacg ataatgccga gg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAEAFTRED YVFMAQLNEN AERYDEMVET MRKISGMEGE LSDKERNLLS VAYKNVIGPR RAAWRIVSSI EAKEKGRQKP NAKRIEQIRV YRQKIEKELS DICNDILKLL QEQFVPRSTN ADAKVFYYKM QGDYYRYLAE YSSGEDKEKI AGSALNAYNS AFEISQQLPP THPIRLGLAL NFSVFYYEIL ASPDRACELA RKAFDAAITD LDKLTEESYK DSTLIMQLLR DNLNLWVTDS AGDDNAEEK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgaggca tttacgcgtg aagactacgt ctttatggct cagctcaacg agaacgccga gcgctatgat gagatggtgg agaccatgag gaagatctcc ggtatggaag gcgagctctc ggataaggag cgaaacctgc tttctgtagc ctacaagaac gtcatcggcc cccgccgcgc agcctggagg atcgtctcct ccatcgaggc gaaggagaag ggccgccaga agccgaacgc caagcgaatc gagcagatcc gtgtgtacag gcagaagata gagaaagagc tttcggacat ctgcaacgac atcctcaagc tcctccagga gcagttcgtc ccccgctcca caaatgccga cgcgaaggtc ttctactaca agatgcaggg agactactat aggtacctcg ccgagtactc gtcgggcgag gacaaggaga agatcgccgg ctctgccctc aacgcctaca actctgcgtt cgagatctcc cagcagctgc ctccgaccca cccgatccgt ctcgggctgg ctctgaactt ctctgtcttc tactatgaga tcctcgcgtc tcctgacagg gcttgcgagc ttgcgcgcaa ggctttcgac gcggccatca cagatctgga caagctgacc gaggagtctt acaaggactc gaccctcatc atgcagctcc tccgcgacaa cctcaacctc tgggtgacgg actctgccgg tgacgataat gccgaggaga agaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for GilaA.00361.a.A1.PW25680
PURIFICATION DATe: 12/8/2008
CONCENTRATION: 27.79mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 17
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAEAFTRED YVFMAQLNEN AERYDEMVET MRKISGMEGE LSDKERNLLS VAYKNVIGPR RAAWRIVSSI EAKEKGRQKP NAKRIEQIRV YRQKIEKELS DICNDILKLL QEQFVPRSTN ADAKVFYYKM QGDYYRYLAE YSSGEDKEKI AGSALNAYNS AFEISQQLPP THPIRLGLAL NFSVFYYEIL ASPDRACELA RKAFDAAITD LDKLTEESYK DSTLIMQLLR DNLNLWVTDS AGDDNAE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggccg aggcatttac gcgtgaagac tacgtcttta tggctcagct caacgagaac gccgagcgct atgatgagat ggtggagacc atgaggaaga tctccggtat ggaaggcgag ctctcggata aggagcgaaa cctgctttct gtagcctaca agaacgtcat cggcccccgc cgcgcagcct ggaggatcgt ctcctccatc gaggcgaagg agaagggccg ccagaagccg aacgccaagc gaatcgagca gatccgtgtg tacaggcaga agatagagaa agagctttcg gacatctgca acgacatcct caagctcctc caggagcagt tcgtcccccg ctccacaaat gccgacgcga aggtcttcta ctacaagatg cagggagact actataggta cctcgccgag tactcgtcgg gcgaggacaa ggagaagatc gccggctctg ccctcaacgc ctacaactct gcgttcgaga tctcccagca gctgcctccg acccacccga tccgtctcgg gctggctctg aacttctctg tcttctacta tgagatcctc gcgtctcctg acagggcttg cgagcttgcg cgcaaggctt tcgacgcggc catcacagat ctggacaagc tgaccgagga gtcttacaag gactcgaccc tcatcatgca gctcctccgc gacaacctca acctctgggt gacggactct gccggtgacg ataatgccga gg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAEAFTRED YVFMAQLNEN AERYDEMVET MRKISGMEGE LSDKERNLLS VAYKNVIGPR RAAWRIVSSI EAKEKGRQKP NAKRIEQIRV YRQKIEKELS DICNDILKLL QEQFVPRSTN ADAKVFYYKM QGDYYRYLAE YSSGEDKEKI AGSALNAYNS AFEISQQLPP THPIRLGLAL NFSVFYYEIL ASPDRACELA RKAFDAAITD LDKLTEESYK DSTLIMQLLR DNLNLWVTDS AGDDNAEEK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGAGGCA TTTACGCGTG AAGACTACGT CTTTATGGCT CAGCTCAACG AGAACGCCGA GCGCTATGAT GAGATGGTGG AGACCATGAG GAAGATCTCC GGTATGGAAG GCGAGCTCTC GGATAAGGAG CGAAACCTGC TTTCTGTAGC CTACAAGAAC GTCATCGGCC CCCGCCGCGC AGCCTGGAGG ATCGTCTCCT CCATCGAGGC GAAGGAGAAG GGCCGCCAGA AGCCGAACGC CAAGCGAATC GAGCAGATCC GTGTGTACAG GCAGAAGATA GAGAAAGAGC TTTCGGACAT CTGCAACGAC ATCCTCAAGC TCCTCCAGGA GCAGTTCGTC CCCCGCTCCA CAAATGCCGA CGCGAAGGTC TTCTACTACA AGATGCAGGG AGACTACTAT AGGTACCTCG CCGAGTACTC GTCGGGCGAG GACAAGGAGA AGATCGCCGG CTCTGCCCTC AACGCCTACA ACTCTGCGTT CGAGATCTCC CAGCAGCTGC CTCCGACCCA CCCGATCCGT CTCGGGCTGG CTCTGAACTT CTCTGTCTTC TACTATGAGA TCCTCGCGTC TCCTGACAGG GCTTGCGAGC TTGCGCGCAA GGCTTTCGAC GCGGCCATCA CAGATCTGGA CAAGCTGACC GAGGAGTCTT ACAAGGACTC GACCCTCATC ATGCAGCTCC TCCGCGACAA CCTCAACCTC TGGGTGACGG ACTCTGCCGG TGACGATAAT GCCGAGGAGA AGAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA