GilaA.00333.a
Cyclin-dependent kinase
| CENTER ID: | GilaA.00333.a |
| ORGANISM: | Giardia lamblia ATCC 50803 |
| ASSOCIATED DISEASE: | Giardiasis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| GilaA.00333.a.A1.GE25628 | Full length( GilaA.00333.a ) | 1 | 308 | ||
| GilaA.00333.a.A1.GE25628 | Full length( GilaA.00333.a ) | 1 | 308 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| GilaA.00333.a.A1.PW25676 | Full length( GilaA.00333.a ) | 1 | 308 | ||
| GilaA.00333.a.A1.PW31843 | Full length( GilaA.00333.a ) | 1 | 308 |
Structures
3GBZ
DEPOSITED: 2/20/2009
DETERMINATION: XRay
CLONE: GilaA.00333.a.A1.GE25628
PROTEIN: GilaA.00333.a.A1.PS00137
3GC0
DEPOSITED: 2/20/2009
DETERMINATION: XRay
CLONE: GilaA.00333.a.A1.GE25628
PROTEIN: GilaA.00333.a.A1.PS00137
Publications by SSGCID
Structure of a cyclin-dependent kinase from Giardia lamblia.
Abendroth J, Guo W, Kelley A, Leibly DJ, Newling PA, Stewart LJ, van Voorhis W
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2011
volume 67, issue Pt 9, pages 1084-9
PMID: 21904054; PMCID: PMC3169406
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | GiardiaDB:GL50803_8037 |
| OrthoMCL: | OG5_126712 |
| RefSeq: | XP_001704058.1 |
| UniProt: | A8BZ95 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVSAAPSAT SIDRYRRITK LGEGTYGEVY KAIDTVTNET VAIKRIRLEH EEEGVPGTAI REVSLLKELQ HRNIIELKSV IHHNHRLHLI FEYAENDLKK YMDKNPDVSM RVIKSFLYQL INGVNFCHSR RCLHRDLKPQ NLLLSVSDAS ETPVLKIGDF GLARAFGIPI RQFTHEIITL WYRPPEILLG SRHYSTSVDI WSIACIWAEM LMKTPLFPGD SEIDQLFKIF EVLGLPDDTT WPGVTALPDW KQSFPKFRGK TLKRVLGALL DDEGLDLLTA MLEMDPVKRI SAKNALEHPY FSHNDFDP
NT Sequence
ATGAGCGTGT CTGCAGCCCC GTCAGCTACC TCAATCGACC GCTATCGGCG GATTACGAAG CTCGGGGAGG GCACGTACGG AGAGGTTTAC AAGGCGATTG ATACGGTCAC GAATGAGACC GTGGCGATTA AAAGGATTCG GCTCGAGCAC GAAGAGGAGG GGGTTCCAGG GACTGCAATT CGGGAGGTGT CTCTCCTTAA GGAGCTCCAG CACCGCAATA TTATCGAGCT CAAGTCCGTT ATCCATCACA ATCACCGTCT CCATCTGATC TTCGAGTATG CCGAGAACGA CCTGAAGAAG TACATGGATA AGAACCCTGA TGTAAGCATG CGCGTGATCA AGTCATTCTT GTATCAGCTC ATCAATGGAG TCAACTTCTG CCACAGCAGA CGCTGCCTCC ACCGAGACCT GAAACCTCAG AATCTGCTCC TCAGCGTCAG CGATGCGTCC GAGACCCCTG TCTTGAAGAT AGGGGACTTT GGCCTGGCGA GAGCGTTCGG CATTCCCATT CGTCAGTTCA CACACGAGAT AATCACACTC TGGTACAGGC CGCCCGAGAT CCTCCTTGGC AGCAGACACT ACAGCACGTC TGTCGACATT TGGTCCATTG CATGCATCTG GGCCGAGATG CTGATGAAGA CACCCCTCTT CCCCGGCGAC AGTGAGATTG ACCAGCTTTT CAAGATATTT GAGGTACTCG GGCTCCCCGA CGACACAACG TGGCCTGGGG TTACGGCCCT CCCAGATTGG AAGCAGAGCT TCCCGAAGTT CCGGGGGAAG ACTCTCAAGA GAGTTTTGGG GGCTCTTCTT GACGATGAAG GACTCGACCT CCTCACTGCC ATGCTCGAGA TGGATCCCGT CAAGCGCATT AGCGCGAAGA ACGCTCTCGA GCACCCGTAC TTCTCCCACA ACGATTTTGA CCCT
Details for GilaA.00333.a.A1.GE25628
| HARVESTED ON: | 10/31/2008 |
| SEQUENCED ON: | 7/20/2010 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 38kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MGTLEAQTQG PGSMSVSAAP SATSIDRYRR ITKLGEGTYG EVYKAIDTVT NETVAIKRIR LEHEEEGVPG TAIREVSLLK ELQHRNIIEL KSVIHHNHRL HLIFEYAEND LKKYMDKNPD VSMRVIKSFL YQLINGVNFC HSRRCLHRDL KPQNLLLSVS DASETPVLKI GDFGLARAFG IPIRQFTHEI ITLWYRPPEI LLGSRHYSTS VDIWSIACIW AEMLMKTPLF PGDSEIDQLF KIFEVLGLPD DTTWPGVTAL PDWKQSFPKF RGKTLKRVLG ALLDDEGLDL LTAMLEMDPV KRISAKNALE HPYFSHNDF
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagcgt gtctgcagcc ccgtcagcta cctcaatcga ccgctatcgg cggattacga agctcgggga gggcacgtac ggagaggttt acaaggcgat tgatacggtc acgaatgaga ccgtggcgat taaaaggatt cggctcgagc acgaagagga gggggttcca gggactgcaa ttcgggaggt gtctctcctt aaggagctcc agcaccgcaa tattatcgag ctcaagtccg ttatccatca caatcaccgt ctccatctga tcttcgagta tgccgagaac gacctgaaga agtacatgga taagaaccct gatgtaagca tgcgcgtgat caagtcattc ttgtatcagc tcatcaatgg agtcaacttc tgccacagca gacgctgcct ccaccgagac ctgaaacctc agaatctgct cctcagcgtc agcgatgcgt ccgagacccc tgtcttgaag ataggggact ttggcctggc gagagcgttc ggcattccca ttcgtcagtt cacacacgag ataatcacac tctggtacag gccgcccgag atcctccttg gcagcagaca ctacagcacg tctgtcgaca tttggtccat tgcatgcatc tgggccgaga tgctgatgaa gacacccctc ttccccggcg acagtgagat tgaccagctt ttcaagatat ttgaggtact cgggctcccc gacgacacaa cgtggcctgg ggttacggcc ctcccagatt ggaagcagag cttcccgaag ttccggggga agactctcaa gagagttttg ggggctcttc ttgacgatga aggactcgac ctcctcactg ccatgctcga gatggatccc gtcaagcgca ttagcgcgaa gaacgctctc gagcacccgt acttctccca caacgatttt g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVSAAPSA TSIDRYRRIT KLGEGTYGEV YKAIDTVTNE TVAIKRIRLE HEEEGVPGTA IREVSLLKEL QHRNIIELKS VIHHNHRLHL IFEYAENDLK KYMDKNPDVS MRVIKSFLYQ LINGVNFCHS RRCLHRDLKP QNLLLSVSDA SETPVLKIGD FGLARAFGIP IRQFTHEIIT LWYRPPEILL GSRHYSTSVD IWSIACIWAE MLMKTPLFPG DSEIDQLFKI FEVLGLPDDT TWPGVTALPD WKQSFPKFRG KTLKRVLGAL LDDEGLDLLT AMLEMDPVKR ISAKNALEHP YFSHNDFDP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgtgtct gcagccccgt cagctacctc aatcgaccgc tatcggcgga ttacgaagct cggggagggc acgtacggag aggtttacaa ggcgattgat acggtcacga atgagaccgt ggcgattaaa aggattcggc tcgagcacga agaggagggg gttccaggga ctgcaattcg ggaggtgtct ctccttaagg agctccagca ccgcaatatt atcgagctca agtccgttat ccatcacaat caccgtctcc atctgatctt cgagtatgcc gagaacgacc tgaagaagta catggataag aaccctgatg taagcatgcg cgtgatcaag tcattcttgt atcagctcat caatggagtc aacttctgcc acagcagacg ctgcctccac cgagacctga aacctcagaa tctgctcctc agcgtcagcg atgcgtccga gacccctgtc ttgaagatag gggactttgg cctggcgaga gcgttcggca ttcccattcg tcagttcaca cacgagataa tcacactctg gtacaggccg cccgagatcc tccttggcag cagacactac agcacgtctg tcgacatttg gtccattgca tgcatctggg ccgagatgct gatgaagaca cccctcttcc ccggcgacag tgagattgac cagcttttca agatatttga ggtactcggg ctccccgacg acacaacgtg gcctggggtt acggccctcc cagattggaa gcagagcttc ccgaagttcc gggggaagac tctcaagaga gttttggggg ctcttcttga cgatgaagga ctcgacctcc tcactgccat gctcgagatg gatcccgtca agcgcattag cgcgaagaac gctctcgagc acccgtactt ctcccacaac gattttgacc ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for GilaA.00333.a.A1.GE25628
| HARVESTED ON: | 10/31/2008 |
| SEQUENCED ON: | 11/24/2008 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 38kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MGTLEAQTQG PGSMSVSAAP SATSIDRYRR ITKLGEGTYG EVYKAIDTVT NETVAIKRIR LEHEEEGVPG TAIREVSLLK ELQHRNIIEL KSVIHHNHRL HLIFEYAEND LKKYMDKNPD VSMRVIKSFL YQLINGVNFC HSRRCLHRDL KPQNLLLSVS DASETPVLKI GDFGLARAFG IPIRQFTHEI ITLWYRPPEI LLGSRHYSTS VDIWSIACIW AEMLMKTPLF PGDSEIDQLF KIFEVLGLPD DTTWPGVTAL PDWKQSFPKF RGKTLKRVLG ALLDDEGLDL LTAMLEMDPV KRISAKNALE HPYFSHNDF
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagcgt gtctgcagcc ccgtcagcta cctcaatcga ccgctatcgg cggattacga agctcgggga gggcacgtac ggagaggttt acaaggcgat tgatacggtc acgaatgaga ccgtggcgat taaaaggatt cggctcgagc acgaagagga gggggttcca gggactgcaa ttcgggaggt gtctctcctt aaggagctcc agcaccgcaa tattatcgag ctcaagtccg ttatccatca caatcaccgt ctccatctga tcttcgagta tgccgagaac gacctgaaga agtacatgga taagaaccct gatgtaagca tgcgcgtgat caagtcattc ttgtatcagc tcatcaatgg agtcaacttc tgccacagca gacgctgcct ccaccgagac ctgaaacctc agaatctgct cctcagcgtc agcgatgcgt ccgagacccc tgtcttgaag ataggggact ttggcctggc gagagcgttc ggcattccca ttcgtcagtt cacacacgag ataatcacac tctggtacag gccgcccgag atcctccttg gcagcagaca ctacagcacg tctgtcgaca tttggtccat tgcatgcatc tgggccgaga tgctgatgaa gacacccctc ttccccggcg acagtgagat tgaccagctt ttcaagatat ttgaggtact cgggctcccc gacgacacaa cgtggcctgg ggttacggcc ctcccagatt ggaagcagag cttcccgaag ttccggggga agactctcaa gagagttttg ggggctcttc ttgacgatga aggactcgac ctcctcactg ccatgctcga gatggatccc gtcaagcgca ttagcgcgaa gaacgctctc gagcacccgt acttctccca caacgatttt g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVSAAPSA TSIDRYRRIT KLGEGTYGEV YKAIDTVTNE TVAIKRIRLE HEEEGVPGTA IREVSLLKEL QHRNIIELKS VIHHNHRLHL IFEYAENDLK KYMDKNPDVS MRVIKSFLYQ LINGVNFCHS RRCLHRDLKP QNLLLSVSDA SETPVLKIGD FGLARAFGIP IRQFTHEIIT LWYRPPEILL GSRHYSTSVD IWSIACIWAE MLMKTPLFPG DSEIDQLFKI FEVLGLPDDT TWPGVTALPD WKQSFPKFRG KTLKRVLGAL LDDEGLDLLT AMLEMDPVKR ISAKNALEHP YFSHNDFDP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgtgtct gcagccccgt cagctacctc aatcgaccgc tatcggcgga ttacgaagct cggggagggc acgtacggag aggtttacaa ggcgattgat acggtcacga atgagaccgt ggcgattaaa aggattcggc tcgagcacga agaggagggg gttccaggga ctgcaattcg ggaggtgtct ctccttaagg agctccagca ccgcaatatt atcgagctca agtccgttat ccatcacaat caccgtctcc atctgatctt cgagtatgcc gagaacgacc tgaagaagta catggataag aaccctgatg taagcatgcg cgtgatcaag tcattcttgt atcagctcat caatggagtc aacttctgcc acagcagacg ctgcctccac cgagacctga aacctcagaa tctgctcctc agcgtcagcg atgcgtccga gacccctgtc ttgaagatag gggactttgg cctggcgaga gcgttcggca ttcccattcg tcagttcaca cacgagataa tcacactctg gtacaggccg cccgagatcc tccttggcag cagacactac agcacgtctg tcgacatttg gtccattgca tgcatctggg ccgagatgct gatgaagaca cccctcttcc ccggcgacag tgagattgac cagcttttca agatatttga ggtactcggg ctccccgacg acacaacgtg gcctggggtt acggccctcc cagattggaa gcagagcttc ccgaagttcc gggggaagac tctcaagaga gttttggggg ctcttcttga cgatgaagga ctcgacctcc tcactgccat gctcgagatg gatcccgtca agcgcattag cgcgaagaac gctctcgagc acccgtactt ctcccacaac gattttgacc ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for GilaA.00333.a.A1.PW25676
| PURIFICATION DATe: | 2/25/2011 |
| CONCENTRATION: | 35.9mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMSVSAAP SATSIDRYRR ITKLGEGTYG EVYKAIDTVT NETVAIKRIR LEHEEEGVPG TAIREVSLLK ELQHRNIIEL KSVIHHNHRL HLIFEYAEND LKKYMDKNPD VSMRVIKSFL YQLINGVNFC HSRRCLHRDL KPQNLLLSVS DASETPVLKI GDFGLARAFG IPIRQFTHEI ITLWYRPPEI LLGSRHYSTS VDIWSIACIW AEMLMKTPLF PGDSEIDQLF KIFEVLGLPD DTTWPGVTAL PDWKQSFPKF RGKTLKRVLG ALLDDEGLDL LTAMLEMDPV KRISAKNALE HPYFSHNDF
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagcgt gtctgcagcc ccgtcagcta cctcaatcga ccgctatcgg cggattacga agctcgggga gggcacgtac ggagaggttt acaaggcgat tgatacggtc acgaatgaga ccgtggcgat taaaaggatt cggctcgagc acgaagagga gggggttcca gggactgcaa ttcgggaggt gtctctcctt aaggagctcc agcaccgcaa tattatcgag ctcaagtccg ttatccatca caatcaccgt ctccatctga tcttcgagta tgccgagaac gacctgaaga agtacatgga taagaaccct gatgtaagca tgcgcgtgat caagtcattc ttgtatcagc tcatcaatgg agtcaacttc tgccacagca gacgctgcct ccaccgagac ctgaaacctc agaatctgct cctcagcgtc agcgatgcgt ccgagacccc tgtcttgaag ataggggact ttggcctggc gagagcgttc ggcattccca ttcgtcagtt cacacacgag ataatcacac tctggtacag gccgcccgag atcctccttg gcagcagaca ctacagcacg tctgtcgaca tttggtccat tgcatgcatc tgggccgaga tgctgatgaa gacacccctc ttccccggcg acagtgagat tgaccagctt ttcaagatat ttgaggtact cgggctcccc gacgacacaa cgtggcctgg ggttacggcc ctcccagatt ggaagcagag cttcccgaag ttccggggga agactctcaa gagagttttg ggggctcttc ttgacgatga aggactcgac ctcctcactg ccatgctcga gatggatccc gtcaagcgca ttagcgcgaa gaacgctctc gagcacccgt acttctccca caacgatttt g
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVSAAPSA TSIDRYRRIT KLGEGTYGEV YKAIDTVTNE TVAIKRIRLE HEEEGVPGTA IREVSLLKEL QHRNIIELKS VIHHNHRLHL IFEYAENDLK KYMDKNPDVS MRVIKSFLYQ LINGVNFCHS RRCLHRDLKP QNLLLSVSDA SETPVLKIGD FGLARAFGIP IRQFTHEIIT LWYRPPEILL GSRHYSTSVD IWSIACIWAE MLMKTPLFPG DSEIDQLFKI FEVLGLPDDT TWPGVTALPD WKQSFPKFRG KTLKRVLGAL LDDEGLDLLT AMLEMDPVKR ISAKNALEHP YFSHNDFDP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGTGTCT GCAGCCCCGT CAGCTACCTC AATCGACCGC TATCGGCGGA TTACGAAGCT CGGGGAGGGC ACGTACGGAG AGGTTTACAA GGCGATTGAT ACGGTCACGA ATGAGACCGT GGCGATTAAA AGGATTCGGC TCGAGCACGA AGAGGAGGGG GTTCCAGGGA CTGCAATTCG GGAGGTGTCT CTCCTTAAGG AGCTCCAGCA CCGCAATATT ATCGAGCTCA AGTCCGTTAT CCATCACAAT CACCGTCTCC ATCTGATCTT CGAGTATGCC GAGAACGACC TGAAGAAGTA CATGGATAAG AACCCTGATG TAAGCATGCG CGTGATCAAG TCATTCTTGT ATCAGCTCAT CAATGGAGTC AACTTCTGCC ACAGCAGACG CTGCCTCCAC CGAGACCTGA AACCTCAGAA TCTGCTCCTC AGCGTCAGCG ATGCGTCCGA GACCCCTGTC TTGAAGATAG GGGACTTTGG CCTGGCGAGA GCGTTCGGCA TTCCCATTCG TCAGTTCACA CACGAGATAA TCACACTCTG GTACAGGCCG CCCGAGATCC TCCTTGGCAG CAGACACTAC AGCACGTCTG TCGACATTTG GTCCATTGCA TGCATCTGGG CCGAGATGCT GATGAAGACA CCCCTCTTCC CCGGCGACAG TGAGATTGAC CAGCTTTTCA AGATATTTGA GGTACTCGGG CTCCCCGACG ACACAACGTG GCCTGGGGTT ACGGCCCTCC CAGATTGGAA GCAGAGCTTC CCGAAGTTCC GGGGGAAGAC TCTCAAGAGA GTTTTGGGGG CTCTTCTTGA CGATGAAGGA CTCGACCTCC TCACTGCCAT GCTCGAGATG GATCCCGTCA AGCGCATTAG CGCGAAGAAC GCTCTCGAGC ACCCGTACTT CTCCCACAAC GATTTTGACC CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA
Details for GilaA.00333.a.A1.PW31843
| PURIFICATION DATe: | 2/25/2011 |
| CONCENTRATION: | 27.8mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMSVSAAP SATSIDRYRR ITKLGEGTYG EVYKAIDTVT NETVAIKRIR LEHEEEGVPG TAIREVSLLK ELQHRNIIEL KSVIHHNHRL HLIFEYAEND LKKYMDKNPD VSMRVIKSFL YQLINGVNFC HSRRCLHRDL KPQNLLLSVS DASETPVLKI GDFGLARAFG IPIRQFTHEI ITLWYRPPEI LLGSRHYSTS VDIWSIACIW AEMLMKTPLF PGDSEIDQLF KIFEVLGLPD DTTWPGVTAL PDWKQSFPKF RGKTLKRVLG ALLDDEGLDL LTAMLEMDPV KRISAKNALE HPYFSHNDF
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagcgt gtctgcagcc ccgtcagcta cctcaatcga ccgctatcgg cggattacga agctcgggga gggcacgtac ggagaggttt acaaggcgat tgatacggtc acgaatgaga ccgtggcgat taaaaggatt cggctcgagc acgaagagga gggggttcca gggactgcaa ttcgggaggt gtctctcctt aaggagctcc agcaccgcaa tattatcgag ctcaagtccg ttatccatca caatcaccgt ctccatctga tcttcgagta tgccgagaac gacctgaaga agtacatgga taagaaccct gatgtaagca tgcgcgtgat caagtcattc ttgtatcagc tcatcaatgg agtcaacttc tgccacagca gacgctgcct ccaccgagac ctgaaacctc agaatctgct cctcagcgtc agcgatgcgt ccgagacccc tgtcttgaag ataggggact ttggcctggc gagagcgttc ggcattccca ttcgtcagtt cacacacgag ataatcacac tctggtacag gccgcccgag atcctccttg gcagcagaca ctacagcacg tctgtcgaca tttggtccat tgcatgcatc tgggccgaga tgctgatgaa gacacccctc ttccccggcg acagtgagat tgaccagctt ttcaagatat ttgaggtact cgggctcccc gacgacacaa cgtggcctgg ggttacggcc ctcccagatt ggaagcagag cttcccgaag ttccggggga agactctcaa gagagttttg ggggctcttc ttgacgatga aggactcgac ctcctcactg ccatgctcga gatggatccc gtcaagcgca ttagcgcgaa gaacgctctc gagcacccgt acttctccca caacgatttt g
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVSAAPSA TSIDRYRRIT KLGEGTYGEV YKAIDTVTNE TVAIKRIRLE HEEEGVPGTA IREVSLLKEL QHRNIIELKS VIHHNHRLHL IFEYAENDLK KYMDKNPDVS MRVIKSFLYQ LINGVNFCHS RRCLHRDLKP QNLLLSVSDA SETPVLKIGD FGLARAFGIP IRQFTHEIIT LWYRPPEILL GSRHYSTSVD IWSIACIWAE MLMKTPLFPG DSEIDQLFKI FEVLGLPDDT TWPGVTALPD WKQSFPKFRG KTLKRVLGAL LDDEGLDLLT AMLEMDPVKR ISAKNALEHP YFSHNDFDP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGTGTCT GCAGCCCCGT CAGCTACCTC AATCGACCGC TATCGGCGGA TTACGAAGCT CGGGGAGGGC ACGTACGGAG AGGTTTACAA GGCGATTGAT ACGGTCACGA ATGAGACCGT GGCGATTAAA AGGATTCGGC TCGAGCACGA AGAGGAGGGG GTTCCAGGGA CTGCAATTCG GGAGGTGTCT CTCCTTAAGG AGCTCCAGCA CCGCAATATT ATCGAGCTCA AGTCCGTTAT CCATCACAAT CACCGTCTCC ATCTGATCTT CGAGTATGCC GAGAACGACC TGAAGAAGTA CATGGATAAG AACCCTGATG TAAGCATGCG CGTGATCAAG TCATTCTTGT ATCAGCTCAT CAATGGAGTC AACTTCTGCC ACAGCAGACG CTGCCTCCAC CGAGACCTGA AACCTCAGAA TCTGCTCCTC AGCGTCAGCG ATGCGTCCGA GACCCCTGTC TTGAAGATAG GGGACTTTGG CCTGGCGAGA GCGTTCGGCA TTCCCATTCG TCAGTTCACA CACGAGATAA TCACACTCTG GTACAGGCCG CCCGAGATCC TCCTTGGCAG CAGACACTAC AGCACGTCTG TCGACATTTG GTCCATTGCA TGCATCTGGG CCGAGATGCT GATGAAGACA CCCCTCTTCC CCGGCGACAG TGAGATTGAC CAGCTTTTCA AGATATTTGA GGTACTCGGG CTCCCCGACG ACACAACGTG GCCTGGGGTT ACGGCCCTCC CAGATTGGAA GCAGAGCTTC CCGAAGTTCC GGGGGAAGAC TCTCAAGAGA GTTTTGGGGG CTCTTCTTGA CGATGAAGGA CTCGACCTCC TCACTGCCAT GCTCGAGATG GATCCCGTCA AGCGCATTAG CGCGAAGAAC GCTCTCGAGC ACCCGTACTT CTCCCACAAC GATTTTGACC CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA