GilaA.00282.a

Glucosamine-6-phosphate deaminase

CENTER ID: GilaA.00282.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.00282.a.A1.GE25624 Full length( GilaA.00282.a ) 1 263
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.00282.a.A1.PW25672 Full length( GilaA.00282.a ) 1 263

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_10829
RefSeq: XP_001709089.1
UniProt: Q549P6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTVCVAQAER GASELESKAM VKKSNDAALL VAHRIAEVVR SKPNCVLGLA TGSTPIPVYQ ELARLHREEG LDFSQVRTFN LDEYAGLPPT HDQSYRFFME EHLFSKVNIK PENVHFLSGL AIRAEDECIR YENALQTIGP CDVWLLGIGH NGHIAFNEPG SPRDSRTRVV CLTQSTIDAN ARFFGNDKSK VPTKALSVGI ATIMESREIL LLATGESKCE AVTKAIVGEP TNAVPASLLQ RHPRCCFYVD EAAGAEVAET VTE
NT Sequence
ATGACTGTGT GTGTTGCTCA GGCGGAGCGT GGTGCGTCAG AGCTGGAGTC TAAGGCAATG GTGAAGAAAA GCAACGATGC TGCGTTACTA GTTGCCCACC GTATCGCGGA AGTTGTCCGC AGCAAGCCGA ATTGCGTTCT GGGCCTGGCG ACCGGATCGA CCCCCATCCC CGTCTACCAG GAGCTCGCCC GTCTCCACCG CGAGGAGGGC CTGGACTTCT CGCAGGTCAG GACGTTCAAC CTGGACGAGT ATGCAGGGCT TCCTCCCACG CACGACCAGA GCTACCGCTT CTTCATGGAG GAGCACCTCT TCAGCAAGGT CAATATAAAG CCGGAGAATG TACACTTCTT GAGCGGACTG GCAATACGGG CTGAGGATGA ATGCATAAGA TATGAAAATG CGCTCCAAAC AATTGGCCCC TGCGACGTCT GGCTTCTGGG GATCGGGCAC AACGGTCACA TCGCCTTCAA CGAGCCGGGG TCCCCGAGAG ACAGCAGGAC GCGCGTCGTC TGCCTGACCC AGAGCACGAT CGACGCCAAC GCGCGCTTCT TCGGAAACGA CAAGTCGAAG GTCCCGACCA AGGCCCTCTC TGTGGGGATA GCAACCATCA TGGAGAGCCG CGAGATCCTT CTTCTCGCCA CCGGTGAGAG CAAGTGTGAG GCTGTGACAA AGGCCATAGT TGGGGAGCCA ACAAATGCTG TTCCTGCCTC GCTGCTTCAA AGACATCCTC GCTGTTGCTT TTATGTCGAT GAGGCCGCAG GTGCGGAAGT CGCCGAGACC GTTACAGAA
Details for GilaA.00282.a.A1.GE25624
HARVESTED ON: 10/17/2008
SEQUENCED ON: 7/28/2010
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTVCVAQAE RGASELESKA MVKKSNDAAL LVAHRIAEVV RSKPNCVLGL ATGSTPIPVY QELARLHREE GLDFSQVRTF NLDEYAGLPP THDQSYRFFM EEHLFSKVNI KPENVHFLSG LAIRAEDECI RYENALQTIG PCDVWLLGIG HNGHIAFNEP GSPRDSRTRV VCLTQSTIDA NARFFGNDKS KVPTKALSVG IATIMESREI LLLATGESKC EAVTKAIVGE PTNAVPASLL QRHPRCCFYV DEAAGAEVAE TVTE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgactg tgtgtgttgc tcaggcggag cgtggtgcgt cagagctgga gtctaaggca atggtgaaga aaagcaacga tgctgcgtta ctagttgccc accgtatcgc ggaagttgtc cgcagcaagc cgaattgcgt tctgggcctg gcgaccggat cgacccccat ccccgtctac caggagctcg cccgtctcca ccgcgaggag ggcctggact tctcgcaggt caggacgttc aacctggacg agtatgcagg gcttcctccc acgcacgacc agagctaccg cttcttcatg gaggagcacc tcttcagcaa ggtcaatata aagccggaga atgtacactt cttgagcgga ctggcaatac gggctgagga tgaatgcata agatatgaaa atgcgctcca aacaattggc ccctgcgacg tctggcttct ggggatcggg cacaacggtc acatcgcctt caacgagccg gggtccccga gagacagcag gacgcgcgtc gtctgcctga cccagagcac gatcgacgcc aacgcgcgct tcttcggaaa cgacaagtcg aaggtcccga ccaaggccct ctctgtgggg atagcaacca tcatggagag ccgcgagatc cttcttctcg ccaccggtga gagcaagtgt gaggctgtga caaaggccat agttggggag ccaacaaatg ctgttcctgc ctcgctgctt caaagacatc ctcgctgttg cttttatgtc gatgaggccg caggtgcgga agtcgccgag accgttacag aataataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVCVAQAE RGASELESKA MVKKSNDAAL LVAHRIAEVV RSKPNCVLGL ATGSTPIPVY QELARLHREE GLDFSQVRTF NLDEYAGLPP THDQSYRFFM EEHLFSKVNI KPENVHFLSG LAIRAEDECI RYENALQTIG PCDVWLLGIG HNGHIAFNEP GSPRDSRTRV VCLTQSTIDA NARFFGNDKS KVPTKALSVG IATIMESREI LLLATGESKC EAVTKAIVGE PTNAVPASLL QRHPRCCFYV DEAAGAEVAE TVTE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactgtgtgt gttgctcagg cggagcgtgg tgcgtcagag ctggagtcta aggcaatggt gaagaaaagc aacgatgctg cgttactagt tgcccaccgt atcgcggaag ttgtccgcag caagccgaat tgcgttctgg gcctggcgac cggatcgacc cccatccccg tctaccagga gctcgcccgt ctccaccgcg aggagggcct ggacttctcg caggtcagga cgttcaacct ggacgagtat gcagggcttc ctcccacgca cgaccagagc taccgcttct tcatggagga gcacctcttc agcaaggtca atataaagcc ggagaatgta cacttcttga gcggactggc aatacgggct gaggatgaat gcataagata tgaaaatgcg ctccaaacaa ttggcccctg cgacgtctgg cttctgggga tcgggcacaa cggtcacatc gccttcaacg agccggggtc cccgagagac agcaggacgc gcgtcgtctg cctgacccag agcacgatcg acgccaacgc gcgcttcttc ggaaacgaca agtcgaaggt cccgaccaag gccctctctg tggggatagc aaccatcatg gagagccgcg agatccttct tctcgccacc ggtgagagca agtgtgaggc tgtgacaaag gccatagttg gggagccaac aaatgctgtt cctgcctcgc tgcttcaaag acatcctcgc tgttgctttt atgtcgatga ggccgcaggt gcggaagtcg ccgagaccgt tacagaaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for GilaA.00282.a.A1.PW25672
PURIFICATION DATe: 10/28/2008
CONCENTRATION: 33.56mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 22
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTVCVAQAE RGASELESKA MVKKSNDAAL LVAHRIAEVV RSKPNCVLGL ATGSTPIPVY QELARLHREE GLDFSQVRTF NLDEYAGLPP THDQSYRFFM EEHLFSKVNI KPENVHFLSG LAIRAEDECI RYENALQTIG PCDVWLLGIG HNGHIAFNEP GSPRDSRTRV VCLTQSTIDA NARFFGNDKS KVPTKALSVG IATIMESREI LLLATGESKC EAVTKAIVGE PTNAVPASLL QRHPRCCFYV DEAAGAEVAE TVTE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgactg tgtgtgttgc tcaggcggag cgtggtgcgt cagagctgga gtctaaggca atggtgaaga aaagcaacga tgctgcgtta ctagttgccc accgtatcgc ggaagttgtc cgcagcaagc cgaattgcgt tctgggcctg gcgaccggat cgacccccat ccccgtctac caggagctcg cccgtctcca ccgcgaggag ggcctggact tctcgcaggt caggacgttc aacctggacg agtatgcagg gcttcctccc acgcacgacc agagctaccg cttcttcatg gaggagcacc tcttcagcaa ggtcaatata aagccggaga atgtacactt cttgagcgga ctggcaatac gggctgagga tgaatgcata agatatgaaa atgcgctcca aacaattggc ccctgcgacg tctggcttct ggggatcggg cacaacggtc acatcgcctt caacgagccg gggtccccga gagacagcag gacgcgcgtc gtctgcctga cccagagcac gatcgacgcc aacgcgcgct tcttcggaaa cgacaagtcg aaggtcccga ccaaggccct ctctgtgggg atagcaacca tcatggagag ccgcgagatc cttcttctcg ccaccggtga gagcaagtgt gaggctgtga caaaggccat agttggggag ccaacaaatg ctgttcctgc ctcgctgctt caaagacatc ctcgctgttg cttttatgtc gatgaggccg caggtgcgga agtcgccgag accgttacag aataataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVCVAQAE RGASELESKA MVKKSNDAAL LVAHRIAEVV RSKPNCVLGL ATGSTPIPVY QELARLHREE GLDFSQVRTF NLDEYAGLPP THDQSYRFFM EEHLFSKVNI KPENVHFLSG LAIRAEDECI RYENALQTIG PCDVWLLGIG HNGHIAFNEP GSPRDSRTRV VCLTQSTIDA NARFFGNDKS KVPTKALSVG IATIMESREI LLLATGESKC EAVTKAIVGE PTNAVPASLL QRHPRCCFYV DEAAGAEVAE TVTE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACTGTGTGT GTTGCTCAGG CGGAGCGTGG TGCGTCAGAG CTGGAGTCTA AGGCAATGGT GAAGAAAAGC AACGATGCTG CGTTACTAGT TGCCCACCGT ATCGCGGAAG TTGTCCGCAG CAAGCCGAAT TGCGTTCTGG GCCTGGCGAC CGGATCGACC CCCATCCCCG TCTACCAGGA GCTCGCCCGT CTCCACCGCG AGGAGGGCCT GGACTTCTCG CAGGTCAGGA CGTTCAACCT GGACGAGTAT GCAGGGCTTC CTCCCACGCA CGACCAGAGC TACCGCTTCT TCATGGAGGA GCACCTCTTC AGCAAGGTCA ATATAAAGCC GGAGAATGTA CACTTCTTGA GCGGACTGGC AATACGGGCT GAGGATGAAT GCATAAGATA TGAAAATGCG CTCCAAACAA TTGGCCCCTG CGACGTCTGG CTTCTGGGGA TCGGGCACAA CGGTCACATC GCCTTCAACG AGCCGGGGTC CCCGAGAGAC AGCAGGACGC GCGTCGTCTG CCTGACCCAG AGCACGATCG ACGCCAACGC GCGCTTCTTC GGAAACGACA AGTCGAAGGT CCCGACCAAG GCCCTCTCTG TGGGGATAGC AACCATCATG GAGAGCCGCG AGATCCTTCT TCTCGCCACC GGTGAGAGCA AGTGTGAGGC TGTGACAAAG GCCATAGTTG GGGAGCCAAC AAATGCTGTT CCTGCCTCGC TGCTTCAAAG ACATCCTCGC TGTTGCTTTT ATGTCGATGA GGCCGCAGGT GCGGAAGTCG CCGAGACCGT TACAGAAAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA