SSGCID: Target Page

FrtuB.18705.a

Modulator of drug activity B

CENTER ID:	FrtuB.18705.a
ORGANISM:	Francisella tularensis subsp. tularensis SCHU S4 / Schu 4
ASSOCIATED DISEASE:	tularemia
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIA

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
FrtuB.18705.a.B1.GE39270	Full length(FrtuB.18705.a)		1	196

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|177416.36.peg.1897
RefSeq:	YP_169951.1
UniProt:	Q5NG91

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKILLINGK KEFGHSKGAL NQYLNDIAYD HLKSLGHDVK LTIVDDDYNI DDEIQKWLWA DTIIYQMPGW WMGPPWIIKK YIDEVFTTGH GVMYASDGRS RYDTSKKYGS GGLLQGKTYM LSLTWNAPFE AFVEKDQLFE GVGVDGVYLH FHKAHEFIGM KGLPTFIAND VMKNPLINEF TLSYKNHLDT VFRNAR

NT Sequence

ATGAAAAAAA TATTATTAAT TAATGGTAAA AAGGAATTTG GGCACTCAAA AGGTGCTTTG AATCAATATC TAAATGATAT CGCTTATGAT CATCTAAAAA GCTTAGGTCA TGATGTTAAA TTAACTATTG TTGATGATGA TTATAATATT GATGATGAAA TTCAGAAGTG GTTATGGGCA GATACTATAA TTTATCAAAT GCCAGGTTGG TGGATGGGAC CACCTTGGAT TATTAAAAAA TATATCGATG AAGTATTCAC TACAGGTCAT GGTGTTATGT ATGCTAGTGA TGGTCGATCT AGGTATGATA CTAGCAAAAA ATATGGCTCT GGTGGTTTAC TTCAGGGTAA AACTTATATG CTGTCGCTAA CATGGAATGC TCCATTCGAG GCTTTTGTTG AGAAAGATCA GCTTTTTGAA GGTGTTGGGG TTGATGGAGT GTATCTACAT TTTCATAAAG CACATGAATT CATAGGTATG AAAGGGTTAC CAACATTTAT TGCTAATGAT GTTATGAAAA ATCCTCTTAT CAACGAATTT ACGTTAAGTT ATAAAAATCA TTTAGATACA GTTTTTAGAA ATGCAAGG

Details for FrtuB.18705.a.B1.GE39270

HARVESTED ON:	6/10/2015
SEQUENCED ON:	6/5/2015
EXPECTED MW:	24kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK KILLINGKKE FGHSKGALNQ YLNDIAYDHL KSLGHDVKLT IVDDDYNIDD EIQKWLWADT IIYQMPGWWM GPPWIIKKYI DEVFTTGHGV MYASDGRSRY DTSKKYGSGG LLQGKTYMLS LTWNAPFEAF VEKDQLFEGV GVDGVYLHFH KAHEFIGMKG LPTFIANDVM KNPLINEFTL SYKNHLDTVF RNAR

Validated NT Sequence

atcttactca cttaccttgc atttctaaaa actgtatcta aatgattttt ataacttaac gtaaattcgt tgataagagg atttttcata acatcattag caataaatgt tggtaaccct ttcataccta tgaattcatg tgctttatga aaatgtagat acactccatc aaccccaaca ccttcaaaaa gctgatcttt ctcaacaaaa gcctcgaatg gagcattcca tgttagcgac agcatataag ttttaccctg aagtaaacca ccagagccat attttttgct agtatcatac ctagatcgac catcactagc atacataaca ccatgacctg tagtgaatac ttcatcgata tattttttaa taatccaagg tggtcccatc caccaacctg gcatttgata aattatagta tctgcccata accacttctg aatttcatca tcaatattat aatcatcatc aacaatagtt aatttaacat catgacctaa gctttttaga tgatcataag cgatatcatt tagatattga ttcaaagcac cttttgagtg cccaaattcc tttttaccat taattaataa tatttttttc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa aaatattatt aattaatggt aaaaaggaat ttgggcactc aaaaggtgct ttgaatcaat atctaaatga tatcgcttat gatcatctaa aaagcttagg tcatgatgtt aaattaacta ttgttgatga tgattataat attgatgatg aaattcagaa gtggttatgg gcagatacta taatttatca aatgccaggt tggtggatgg gaccaccttg gattattaaa aaatatatcg atgaagtatt cactacaggt catggtgtta tgtatgctag tgatggtcga tctaggtatg atactagcaa aaaatatggc tctggtggtt tacttcaggg taaaacttat atgctgtcgc taacatggaa tgctccattc gaggcttttg ttgagaaaga tcagcttttt gaaggtgttg gggttgatgg agtgtatcta cattttcata aagcacatga attcataggt atgaaagggt taccaacatt tattgctaat gatgttatga aaaatcctct tatcaacgaa tttacgttaa gttataaaaa tcatttagat acagttttta gaaatgcaag gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat