FrtuB.18701.a

Putative uncharacterized protein

CENTER ID: FrtuB.18701.a
ORGANISM: Francisella tularensis subsp. tularensis SCHU S4 / Schu 4
ASSOCIATED DISEASE: tularemia
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIA

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
FrtuB.18701.a.B1.GE39268 Full length(FrtuB.18701.a) 1 259
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
FrtuB.18701.a.B1.PS02526 Full length(FrtuB.18701.a) 1 259

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|177416.36.peg.284
RefSeq: YP_169579.1
UniProt: Q5NHB3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVDILDKLW DQYIRDNPHA KEIYDLFVNN GENPINDHIA LRTLDDDRIN IYKLAEVFID KGYSICDDYD FAVKHLEHSD KTQPKVFISQ LLTNEFSEEL QQTLKRCVDL IPQQLLNNPE NLLLSGVSWQ IDYATYQKLL EESEYAAWFY AFGFRANHFT VFINNLKKFS EVSQVNTFLK ENGFRLNSSG GEIKGSKDEL LEQSSTMSGL AEVNFKDGKR EIPSCYYEFA KRYPDNSGKL YQGFVAKSAD KIFESTNTK
NT Sequence
ATGAGCGTTG ATATTTTAGA TAAGTTATGG GATCAATATA TTCGTGATAA TCCCCATGCA AAAGAAATTT ATGATCTGTT TGTAAATAAT GGCGAGAATC CTATAAATGA TCATATTGCA TTAAGAACTT TAGATGATGA TAGGATAAAT ATTTATAAGC TAGCAGAAGT TTTTATTGAT AAAGGCTATA GTATTTGTGA TGATTATGAT TTTGCAGTAA AACATCTTGA GCATAGTGAT AAAACTCAAC CAAAAGTATT TATTAGTCAG CTTTTAACAA ATGAATTCTC AGAGGAATTA CAGCAGACAC TAAAAAGATG TGTTGATCTA ATACCACAGC AATTGCTGAA TAATCCAGAA AATTTACTAT TATCTGGTGT AAGCTGGCAA ATTGATTATG CTACATACCA AAAGCTGCTT GAAGAATCTG AATATGCAGC ATGGTTCTAT GCTTTTGGCT TTAGAGCTAA TCACTTTACA GTATTTATTA ATAATTTGAA AAAATTTAGT GAGGTTTCTC AAGTAAATAC TTTTCTTAAA GAAAATGGAT TTAGGCTTAA CTCATCAGGT GGTGAAATCA AGGGCTCAAA GGACGAATTA CTTGAACAAT CAAGTACCAT GTCAGGTTTA GCTGAGGTTA ATTTTAAGGA TGGTAAGCGA GAGATTCCTT CATGTTATTA TGAATTCGCT AAGAGATATC CTGACAATTC AGGTAAGCTC TACCAAGGTT TTGTTGCTAA ATCTGCAGAT AAAATATTCG AAAGTACAAA TACTAAA
Details for FrtuB.18701.a.B1.GE39268
HARVESTED ON: 6/10/2015
SEQUENCED ON: 6/5/2015
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS VDILDKLWDQ YIRDNPHAKE IYDLFVNNGE NPINDHIALR TLDDDRINIY KLAEVFIDKG YSICDDYDFA VKHLEHSDKT QPKVFISQLL TNEFSEELQQ TLKRCVDLIP QQLLNNPENL LLSGVSWQID YATYQKLLEE SEYAAWFYAF GFRANHFTVF INNLKKFSEV SQVNTFLKEN GFRLNSSGGE IKGSKDELLE QSSTMSGLAE VNFKDGKREI PSCYYEFAKR YPDNSGKLYQ GFVAKSADKI XXSTNTK
Validated NT Sequence
atcttactca cttatttagt atttgtactt tnnaatattt tatctgcaga tttagcaaca aaaccttggt agagcttacc tgaattgtca ggatatctct tagcgaattc ataataacat gaaggaatct ctcgcttacc atccttaaaa ttaacctcag ctaaacctga catggtactt gattgttcaa gtaattcgtc ctttgagccc ttgatttcac cacctgatga gttaagccta aatccatttt ctttaagaaa agtatttact tgagaaacct cactaaattt tttcaaatta ttaataaata ctgtaaagtg attagctcta aagccaaaag catagaacca tgctgcatat tcagattctt caagcagctt ttggtatgta gcataatcaa tttgccagct tacaccagat aatagtaaat tttctggatt attcagcaat tgctgtggta ttagatcaac acatcttttt agtgtctgct gtaattcctc tgagaattca tttgttaaaa gctgactaat aaatactttt ggttgagttt tatcactatg ctcaagatgt tttactgcaa aatcataatc atcacaaata ctatagcctt tatcaataaa aacttctgct agcttataaa tatttatcct atcatcatct aaagttctta atgcaatatg atcatttata ggattctcgc cattatttac aaacagatca taaatttctt ttgcatgggg attatcacga atatattgat cccataactt atctaaaata tcaacgctca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMS VDILDKLWDQ YIRDNPHAKE IYDLFVNNGE NPINDHIALR TLDDDRINIY KLAEVFIDKG YSICDDYDFA VKHLEHSDKT QPKVFISQLL TNEFSEELQQ TLKRCVDLIP QQLLNNPENL LLSGVSWQID YATYQKLLEE SEYAAWFYAF GFRANHFTVF INNLKKFSEV SQVNTFLKEN GFRLNSSGGE IKGSKDELLE QSSTMSGLAE VNFKDGKREI PSCYYEFAKR YPDNSGKLYQ GFVAKSADKI FESTNTK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg ttgatatttt agataagtta tgggatcaat atattcgtga taatccccat gcaaaagaaa tttatgatct gtttgtaaat aatggcgaga atcctataaa tgatcatatt gcattaagaa ctttagatga tgataggata aatatttata agctagcaga agtttttatt gataaaggct atagtatttg tgatgattat gattttgcag taaaacatct tgagcatagt gataaaactc aaccaaaagt atttattagt cagcttttaa caaatgaatt ctcagaggaa ttacagcaga cactaaaaag atgtgttgat ctaataccac agcaattgct gaataatcca gaaaatttac tattatctgg tgtaagctgg caaattgatt atgctacata ccaaaagctg cttgaagaat ctgaatatgc agcatggttc tatgcttttg gctttagagc taatcacttt acagtattta ttaataattt gaaaaaattt agtgaggttt ctcaagtaaa tacttttctt aaagaaaatg gatttaggct taactcatca ggtggtgaaa tcaagggctc aaaggacgaa ttacttgaac aatcaagtac catgtcaggt ttagctgagg ttaattttaa ggatggtaag cgagagattc cttcatgtta ttatgaattc gctaagagat atcctgacaa ttcaggtaag ctctaccaag gttttgttgc taaatctgca gataaaatat tcgaaagtac aaatactaaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for FrtuB.18701.a.B1.PS02526
PURIFICATION DATe: 10/27/2015
CONCENTRATION: 40.37mg/ml
OBSERVED MW: 28kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS VDILDKLWDQ YIRDNPHAKE IYDLFVNNGE NPINDHIALR TLDDDRINIY KLAEVFIDKG YSICDDYDFA VKHLEHSDKT QPKVFISQLL TNEFSEELQQ TLKRCVDLIP QQLLNNPENL LLSGVSWQID YATYQKLLEE SEYAAWFYAF GFRANHFTVF INNLKKFSEV SQVNTFLKEN GFRLNSSGGE IKGSKDELLE QSSTMSGLAE VNFKDGKREI PSCYYEFAKR YPDNSGKLYQ GFVAKSADKI XXSTNTK
Validated NT Sequence
atcttactca cttatttagt atttgtactt tnnaatattt tatctgcaga tttagcaaca aaaccttggt agagcttacc tgaattgtca ggatatctct tagcgaattc ataataacat gaaggaatct ctcgcttacc atccttaaaa ttaacctcag ctaaacctga catggtactt gattgttcaa gtaattcgtc ctttgagccc ttgatttcac cacctgatga gttaagccta aatccatttt ctttaagaaa agtatttact tgagaaacct cactaaattt tttcaaatta ttaataaata ctgtaaagtg attagctcta aagccaaaag catagaacca tgctgcatat tcagattctt caagcagctt ttggtatgta gcataatcaa tttgccagct tacaccagat aatagtaaat tttctggatt attcagcaat tgctgtggta ttagatcaac acatcttttt agtgtctgct gtaattcctc tgagaattca tttgttaaaa gctgactaat aaatactttt ggttgagttt tatcactatg ctcaagatgt tttactgcaa aatcataatc atcacaaata ctatagcctt tatcaataaa aacttctgct agcttataaa tatttatcct atcatcatct aaagttctta atgcaatatg atcatttata ggattctcgc cattatttac aaacagatca taaatttctt ttgcatgggg attatcacga atatattgat cccataactt atctaaaata tcaacgctca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMS VDILDKLWDQ YIRDNPHAKE IYDLFVNNGE NPINDHIALR TLDDDRINIY KLAEVFIDKG YSICDDYDFA VKHLEHSDKT QPKVFISQLL TNEFSEELQQ TLKRCVDLIP QQLLNNPENL LLSGVSWQID YATYQKLLEE SEYAAWFYAF GFRANHFTVF INNLKKFSEV SQVNTFLKEN GFRLNSSGGE IKGSKDELLE QSSTMSGLAE VNFKDGKREI PSCYYEFAKR YPDNSGKLYQ GFVAKSADKI FESTNTK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG TTGATATTTT AGATAAGTTA TGGGATCAAT ATATTCGTGA TAATCCCCAT GCAAAAGAAA TTTATGATCT GTTTGTAAAT AATGGCGAGA ATCCTATAAA TGATCATATT GCATTAAGAA CTTTAGATGA TGATAGGATA AATATTTATA AGCTAGCAGA AGTTTTTATT GATAAAGGCT ATAGTATTTG TGATGATTAT GATTTTGCAG TAAAACATCT TGAGCATAGT GATAAAACTC AACCAAAAGT ATTTATTAGT CAGCTTTTAA CAAATGAATT CTCAGAGGAA TTACAGCAGA CACTAAAAAG ATGTGTTGAT CTAATACCAC AGCAATTGCT GAATAATCCA GAAAATTTAC TATTATCTGG TGTAAGCTGG CAAATTGATT ATGCTACATA CCAAAAGCTG CTTGAAGAAT CTGAATATGC AGCATGGTTC TATGCTTTTG GCTTTAGAGC TAATCACTTT ACAGTATTTA TTAATAATTT GAAAAAATTT AGTGAGGTTT CTCAAGTAAA TACTTTTCTT AAAGAAAATG GATTTAGGCT TAACTCATCA GGTGGTGAAA TCAAGGGCTC AAAGGACGAA TTACTTGAAC AATCAAGTAC CATGTCAGGT TTAGCTGAGG TTAATTTTAA GGATGGTAAG CGAGAGATTC CTTCATGTTA TTATGAATTC GCTAAGAGAT ATCCTGACAA TTCAGGTAAG CTCTACCAAG GTTTTGTTGC TAAATCTGCA GATAAAATAT TCGAAAGTAC AAATACTAAA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT