FrtuB.18699.a

Putative uncharacterized protein

CENTER ID: FrtuB.18699.a
ORGANISM: Francisella tularensis subsp. tularensis SCHU S4 / Schu 4
ASSOCIATED DISEASE: tularemia
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIA

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
FrtuB.18699.a.B1.GE39267 Full length(FrtuB.18699.a) 1 325
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
FrtuB.18699.a.B1.PS02560 Full length(FrtuB.18699.a) 1 325

External Resources

RESOURCE REFERENCE ID
UniProt: Q5NHD9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARVSKMNMI MHGDGHNGIH HNDGLLNVNG IFRNRFDVIL TNPPFGTNLG KDNSKVSEED KYTDEKMITH YKKIYGDVYE EELKQVTDNF GKPIRSLYKT GEISVATEVL FVERCLDLLK AGGRMGIVLP EGVLNSSNLQ KAREYFESRA KILLIVSLPQ DLFVSSGATV KTSLVFLKKF TVEEQEQYET VKTQAEKEAE QKYQPQLLEI QQKIDFEKSI KQHITKLRKA LKTKTAKNKE NLQLTLDETI VEHTEYKKKV KQYKAQLKEL EAKQQEEAKQ LIKQKFDYEI PIAEIEKAGV STIGAEIDNQ LPELLKEFKE YYKRK
NT Sequence
ATGGCAAGAG TATCTAAAAT GAATATGATA ATGCATGGAG ATGGGCATAA TGGTATTCAT CATAATGATG GTTTGCTAAA TGTAAATGGT ATTTTTAGAA ATCGTTTTGA TGTGATTTTG ACAAATCCGC CATTTGGTAC AAATCTTGGT AAAGATAATT CTAAAGTCTC AGAAGAAGAT AAATATACCG ATGAAAAGAT GATAACACAC TATAAAAAAA TCTATGGTGA TGTTTATGAA GAGGAGCTAA AACAGGTTAC AGATAACTTT GGTAAACCAA TCAGAAGTTT ATACAAAACT GGTGAAATAT CAGTAGCAAC AGAAGTTCTA TTTGTTGAGA GATGTTTAGA CTTACTAAAA GCTGGCGGTA GAATGGGTAT AGTTTTGCCA GAAGGTGTAC TAAATAGTTC AAACTTACAA AAAGCTAGAG AATATTTTGA AAGTAGAGCA AAAATATTGC TAATAGTTTC ATTACCTCAA GATTTATTTG TAAGCTCAGG AGCAACTGTA AAGACTAGTT TAGTCTTTTT GAAAAAGTTT ACAGTAGAAG AGCAAGAGCA ATATGAAACT GTAAAAACTC AAGCGGAGAA AGAAGCAGAG CAAAAATACC AACCTCAATT ATTAGAAATA CAACAAAAAA TAGATTTTGA AAAAAGTATT AAACAACATA TTACTAAACT AAGAAAAGCT TTAAAAACAA AAACTGCAAA AAATAAAGAA AACTTACAGC TTACTTTAGA TGAAACTATT GTCGAACATA CTGAGTATAA AAAGAAGGTA AAACAATACA AAGCACAATT AAAAGAGCTA GAAGCTAAAC AACAAGAAGA GGCCAAACAG CTGATTAAAC AAAAATTTGA TTATGAAATA CCTATAGCAG AAATTGAAAA AGCTGGAGTA AGCACAATAG GTGCCGAGAT AGATAATCAG TTACCAGAAC TTCTAAAAGA GTTTAAAGAA TATTATAAAA GAAAA
Details for FrtuB.18699.a.B1.GE39267
HARVESTED ON: 6/10/2015
SEQUENCED ON: 6/5/2015
EXPECTED MW: 38kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHMAR VSKMNMIMHG DGHNGIHHND GLLNVNGIFR NRFDVILTNP PFGTNLGKDN SKVSEEDKYT DEKMITHYKK IYGDVYEEEL KQVTDNFGKP IRSLYKTGEI SVATEVLFVE RCLDLLKAGG RMGIVLPEGV LNSSNLQKAR EYFESRAKIL LIVSLPQDLF VSSGATVKTS LVFLKKFTVE EQEQYETVKT QAEKEAEQKY QPQLLEIQQK IDFEKSIKQH ITKLRKALKT KTAKNKENLQ LTLDETIVEH TEYKKKVKQY KAQLKELEAK QQEEAKQLIK QKFDYEIPIA EIEKAGVSTI GAEIDNQLPE LLK
Validated NT Sequence
tcttttagaa gttctggtaa ctgattatct atctcggcac ctattgtgct tactccagct ttttcaattt ctgctatagg tatttcataa tcaaattttt gtttaatcag ctgtttggcc tcttcttgtt gtttagcttc tagctctttt aattgtgctt tgtattgttt taccttcttt ttatactcag tatgttcgac aatagtttca tctaaagtaa gctgtaagtt ttctttattt tttgcagttt ttgtttttaa agcttttctt agtttagtaa tatgttgttt aatacttttt tcaaaatcta ttttttgttg tatttctaat aattgaggtt ggtatttttg ctctgcttct ttctccgctt gagtttttac agtttcatat tgctcttgct cttctactgt aaactttttc aaaaagacta aactagtctt tacagttgct cctgagctta caaataaatc ttgaggtaat gaaactatta gcaatatttt tgctctactt tcaaaatatt ctctagcttt ttgtaagttt gaactattta gtacaccttc tggcaaaact atacccattc taccgccagc ttttagtaag tctaaacatc tctcaacaaa tagaacttct gttgctactg atatttcacc agttttgtat aaacttctga ttggtttacc aaagttatct gtaacctgtt ttagctcctc ttcataaaca tcaccataga tttttttata gtgtgttatc atcttttcat cggtatattt atcttcttct gagactttag aattatcttt accaagattt gtaccaaatg gcggatttgt caaaatcaca tcaaaacgat ttctaaaaat accatttaca tttagcaaac catcattatg atgaatacca ttatgcccat ctccatgcat tatcatattc attttagata ctcttgccat atggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMA RVSKMNMIMH GDGHNGIHHN DGLLNVNGIF RNRFDVILTN PPFGTNLGKD NSKVSEEDKY TDEKMITHYK KIYGDVYEEE LKQVTDNFGK PIRSLYKTGE ISVATEVLFV ERCLDLLKAG GRMGIVLPEG VLNSSNLQKA REYFESRAKI LLIVSLPQDL FVSSGATVKT SLVFLKKFTV EEQEQYETVK TQAEKEAEQK YQPQLLEIQQ KIDFEKSIKQ HITKLRKALK TKTAKNKENL QLTLDETIVE HTEYKKKVKQ YKAQLKELEA KQQEEAKQLI KQKFDYEIPI AEIEKAGVST IGAEIDNQLP ELLKEFKEYY KRK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa gagtatctaa aatgaatatg ataatgcatg gagatgggca taatggtatt catcataatg atggtttgct aaatgtaaat ggtattttta gaaatcgttt tgatgtgatt ttgacaaatc cgccatttgg tacaaatctt ggtaaagata attctaaagt ctcagaagaa gataaatata ccgatgaaaa gatgataaca cactataaaa aaatctatgg tgatgtttat gaagaggagc taaaacaggt tacagataac tttggtaaac caatcagaag tttatacaaa actggtgaaa tatcagtagc aacagaagtt ctatttgttg agagatgttt agacttacta aaagctggcg gtagaatggg tatagttttg ccagaaggtg tactaaatag ttcaaactta caaaaagcta gagaatattt tgaaagtaga gcaaaaatat tgctaatagt ttcattacct caagatttat ttgtaagctc aggagcaact gtaaagacta gtttagtctt tttgaaaaag tttacagtag aagagcaaga gcaatatgaa actgtaaaaa ctcaagcgga gaaagaagca gagcaaaaat accaacctca attattagaa atacaacaaa aaatagattt tgaaaaaagt attaaacaac atattactaa actaagaaaa gctttaaaaa caaaaactgc aaaaaataaa gaaaacttac agcttacttt agatgaaact attgtcgaac atactgagta taaaaagaag gtaaaacaat acaaagcaca attaaaagag ctagaagcta aacaacaaga agaggccaaa cagctgatta aacaaaaatt tgattatgaa atacctatag cagaaattga aaaagctgga gtaagcacaa taggtgccga gatagataat cagttaccag aacttctaaa agagtttaaa gaatattata aaagaaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for FrtuB.18699.a.B1.PS02560
PURIFICATION DATe: 12/29/2015
CONCENTRATION: 20.31mg/ml
OBSERVED MW: 36kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHMAR VSKMNMIMHG DGHNGIHHND GLLNVNGIFR NRFDVILTNP PFGTNLGKDN SKVSEEDKYT DEKMITHYKK IYGDVYEEEL KQVTDNFGKP IRSLYKTGEI SVATEVLFVE RCLDLLKAGG RMGIVLPEGV LNSSNLQKAR EYFESRAKIL LIVSLPQDLF VSSGATVKTS LVFLKKFTVE EQEQYETVKT QAEKEAEQKY QPQLLEIQQK IDFEKSIKQH ITKLRKALKT KTAKNKENLQ LTLDETIVEH TEYKKKVKQY KAQLKELEAK QQEEAKQLIK QKFDYEIPIA EIEKAGVSTI GAEIDNQLPE LLK
Validated NT Sequence
tcttttagaa gttctggtaa ctgattatct atctcggcac ctattgtgct tactccagct ttttcaattt ctgctatagg tatttcataa tcaaattttt gtttaatcag ctgtttggcc tcttcttgtt gtttagcttc tagctctttt aattgtgctt tgtattgttt taccttcttt ttatactcag tatgttcgac aatagtttca tctaaagtaa gctgtaagtt ttctttattt tttgcagttt ttgtttttaa agcttttctt agtttagtaa tatgttgttt aatacttttt tcaaaatcta ttttttgttg tatttctaat aattgaggtt ggtatttttg ctctgcttct ttctccgctt gagtttttac agtttcatat tgctcttgct cttctactgt aaactttttc aaaaagacta aactagtctt tacagttgct cctgagctta caaataaatc ttgaggtaat gaaactatta gcaatatttt tgctctactt tcaaaatatt ctctagcttt ttgtaagttt gaactattta gtacaccttc tggcaaaact atacccattc taccgccagc ttttagtaag tctaaacatc tctcaacaaa tagaacttct gttgctactg atatttcacc agttttgtat aaacttctga ttggtttacc aaagttatct gtaacctgtt ttagctcctc ttcataaaca tcaccataga tttttttata gtgtgttatc atcttttcat cggtatattt atcttcttct gagactttag aattatcttt accaagattt gtaccaaatg gcggatttgt caaaatcaca tcaaaacgat ttctaaaaat accatttaca tttagcaaac catcattatg atgaatacca ttatgcccat ctccatgcat tatcatattc attttagata ctcttgccat atggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHMA RVSKMNMIMH GDGHNGIHHN DGLLNVNGIF RNRFDVILTN PPFGTNLGKD NSKVSEEDKY TDEKMITHYK KIYGDVYEEE LKQVTDNFGK PIRSLYKTGE ISVATEVLFV ERCLDLLKAG GRMGIVLPEG VLNSSNLQKA REYFESRAKI LLIVSLPQDL FVSSGATVKT SLVFLKKFTV EEQEQYETVK TQAEKEAEQK YQPQLLEIQQ KIDFEKSIKQ HITKLRKALK TKTAKNKENL QLTLDETIVE HTEYKKKVKQ YKAQLKELEA KQQEEAKQLI KQKFDYEIPI AEIEKAGVST IGAEIDNQLP ELLKEFKEYY KRK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAA GAGTATCTAA AATGAATATG ATAATGCATG GAGATGGGCA TAATGGTATT CATCATAATG ATGGTTTGCT AAATGTAAAT GGTATTTTTA GAAATCGTTT TGATGTGATT TTGACAAATC CGCCATTTGG TACAAATCTT GGTAAAGATA ATTCTAAAGT CTCAGAAGAA GATAAATATA CCGATGAAAA GATGATAACA CACTATAAAA AAATCTATGG TGATGTTTAT GAAGAGGAGC TAAAACAGGT TACAGATAAC TTTGGTAAAC CAATCAGAAG TTTATACAAA ACTGGTGAAA TATCAGTAGC AACAGAAGTT CTATTTGTTG AGAGATGTTT AGACTTACTA AAAGCTGGCG GTAGAATGGG TATAGTTTTG CCAGAAGGTG TACTAAATAG TTCAAACTTA CAAAAAGCTA GAGAATATTT TGAAAGTAGA GCAAAAATAT TGCTAATAGT TTCATTACCT CAAGATTTAT TTGTAAGCTC AGGAGCAACT GTAAAGACTA GTTTAGTCTT TTTGAAAAAG TTTACAGTAG AAGAGCAAGA GCAATATGAA ACTGTAAAAA CTCAAGCGGA GAAAGAAGCA GAGCAAAAAT ACCAACCTCA ATTATTAGAA ATACAACAAA AAATAGATTT TGAAAAAAGT ATTAAACAAC ATATTACTAA ACTAAGAAAA GCTTTAAAAA CAAAAACTGC AAAAAATAAA GAAAACTTAC AGCTTACTTT AGATGAAACT ATTGTCGAAC ATACTGAGTA TAAAAAGAAG GTAAAACAAT ACAAAGCACA ATTAAAAGAG CTAGAAGCTA AACAACAAGA AGAGGCCAAA CAGCTGATTA AACAAAAATT TGATTATGAA ATACCTATAG CAGAAATTGA AAAAGCTGGA GTAAGCACAA TAGGTGCCGA GATAGATAAT CAGTTACCAG AACTTCTAAA AGAGTTTAAA GAATATTATA AAAGAAAATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT