SSGCID: Target Page

FrtuB.17449.a

Putative uncharacterized protein

CENTER ID:	FrtuB.17449.a
ORGANISM:	Francisella tularensis subsp. tularensis SCHU S4 / Schu 4
ASSOCIATED DISEASE:	tularemia
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIA

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
FrtuB.17449.a.B1.GE35805	full length		1	268

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|177416.36.peg.400
RefSeq:	YP_169491.1
UniProt:	Q5NHK1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVKKIIICAD DFGMSDNINS AIINLLEKKV INATSCMPNM PAFKLGIAQL KKIYNDFSHV GIHLNLTEGN AFTNPKSITR NGKFLSLSKL LVKSKLRAIN YDDVYNELKA QINNFIEDWG ALPDFIDGHQ HVHHFPIIRK AVINLYKDFN MYTKQTYIRS TYKMDKSDFK SLIIYRSGAK KFYNMLIKNN IKHNSSFAGV YSLESDNQDF RKVILEAYAK IKDGGIIMCH PAADIDIKDP ISQSRIKEFA YFNSKQALQD QKYHNIVL

NT Sequence

atggttaaaa aaataataat ttgtgcagat gactttggca tgagtgataa tataaatagt gctattatta acctcttgga aaaaaaggtt attaatgcca ctagctgtat gccaaatatg cctgcattca aattaggaat tgctcaatta aaaaaaatat ataatgattt tagtcatgtt ggtatacatt taaatttaac tgaaggtaat gcttttacaa atccaaaatc tataactaga aatggcaaat ttttgtcact atcaaaactt cttgtcaaat caaaacttag agcaataaat tatgatgatg tttataatga gctgaaagct caaataaata actttatcga agactggggc gcgctgccag attttattga tggtcatcaa catgttcatc attttccaat aattagaaaa gctgttataa atctatataa agatttcaat atgtacacta agcagaccta tatccgctca acatataaaa tggataaatc ggactttaag tcactaataa tctatcgtag cggtgcaaaa aagttctata atatgctaat aaaaaacaat atcaaacata actcaagttt tgccggagtt tattcgttag aaagtgataa tcaagacttt agaaaagtta ttctagaggc ttatgccaaa attaaagatg gaggtataat aatgtgccat cccgctgcgg atattgatat aaaagatcca atatcacagt cacgcattaa agaatttgct tactttaact ccaaacaagc actacaagat caaaaatatc ataatattgt actataa

Details for FrtuB.17449.a.B1.GE35805

HARVESTED ON:	7/31/2012
SEQUENCED ON:	8/3/2012
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV KKIIICADDF GMSDNINSAI INLLEKKVIN ATSCMPNMPA FKLGIAQLKK IYNDFSHVGI HLNLTEGNAF TNPKSITRNG KFLSLSKLLV KSKLRAINYD DVYNELKAQI NNFIEDWGAL PDFIDGHQHV HHFPIIRKAV INLYKDFNMY TKQTYIRSTY KMDKSDFKSL IIYRSGAKKF YNMLIKNNIK HNSSFAGVYS LESDNQDFRK VILEAYAKIK DGGIIMCHXX ADIDIKDPIS QSRIKEFAYF NSXQALQDQK YHNIVL

Validated NT Sequence

tttnnnnnnn ttaagaagga gatataccat ggctcaccac caccaccacc atatggttaa aaaaataata atttgtgcag atgactttgg catgagtgat aatataaata gtgctattat taacctcttg gaaaaaaagg ttattaatgc cactagctgt atgccaaata tgcctgcatt caaattagga attgctcaat taaaaaaaat atataatgat tttagtcatg ttggtataca tttaaattta actgaaggta atgcttttac aaatccaaaa tctataacta gaaatggcaa atttttgtca ctatcaaaac ttcttgtcaa atcaaaactt agagcaataa attatgatga tgtttataat gagctgaaag ctcaaataaa taactttatc gaagactggg gcgcgctgcc agattttatt gatggtcatc aacatgttca tcattttcca ataattagaa aagctgttat aaatctatat aaagatttca atatgtacac taagcagacc tatatccgct caacatataa aatggataaa tcggacttta agtcactaat aatctatcgt agcggtgcaa aaaagttcta taatatgcta ataaaaaaca atatcaaaca taactcaagt tttgccggag tttattcgtt agaaagtgat aatcaagact ttagaaaagt tattctagag gcttatgcca aaattaaaga tggaggtata ataatgtgcc atnnnnctgc ggatattgat ataaaagatc cnatatcaca gtcacgcatt aaagaatttg cttactttaa ctccnaacaa gcactacaag atcaaaaata tcataatatt gtactataag tgagtaaata ggatncnnnn nnnnnnnncc nnnnnnnnaa nnnnnnnttn tnnannntnc nnannncgnn annnnnannn nta

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtta aaaaaataat aatttgtgca gatgactttg gcatgagtga taatataaat agtgctatta ttaacctctt ggaaaaaaag gttattaatg ccactagctg tatgccaaat atgcctgcat tcaaattagg aattgctcaa ttaaaaaaaa tatataatga ttttagtcat gttggtatac atttaaattt aactgaaggt aatgctttta caaatccaaa atctataact agaaatggca aatttttgtc actatcaaaa cttcttgtca aatcaaaact tagagcaata aattatgatg atgtttataa tgagctgaaa gctcaaataa ataactttat cgaagactgg ggcgcgctgc cagattttat tgatggtcat caacatgttc atcattttcc aataattaga aaagctgtta taaatctata taaagatttc aatatgtaca ctaagcagac ctatatccgc tcaacatata aaatggataa atcggacttt aagtcactaa taatctatcg tagcggtgca aaaaagttct ataatatgct aataaaaaac aatatcaaac ataactcaag ttttgccgga gtttattcgt tagaaagtga taatcaagac tttagaaaag ttattctaga ggcttatgcc aaaattaaag atggaggtat aataatgtgc catcccgctg cggatattga tataaaagat ccaatatcac agtcacgcat taaagaattt gcttacttta actccaaaca agcactacaa gatcaaaaat atcataatat tgtactatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at