FrtuB.17360.a

Putative uncharacterized protein

CENTER ID: FrtuB.17360.a
ORGANISM: Francisella tularensis subsp. tularensis SCHU S4 / Schu 4
ASSOCIATED DISEASE: tularemia
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIA

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
FrtuB.17360.a.B1.GE39266 Full length(FrtuB.17360.a) 1 327
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|177416.36.peg.1898
RefSeq: YP_169950.1
UniProt: Q5NG92

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQASMHASEL QGNAVMIELL EYFKKYQPKG DVYLIPQCNP IGKDGFIGAG HQGRFDSATG DNFNRYYFYP KIDYSAFVKE HINSTTAEYK QAFENLLQQE LDKELSNEWE LSRAKRLNLL VQREAQKADY VLDLHTDTDA TTYLYTADFA RQTAEKFGYK DMLIIDNDKA SGALDEAIFC PWWHLAQEFK KQDRDEKVLK EGYTLELCSE EYINFANAKM QTQGILNYLK HKDIIDSPFD TDLLNTDIRH HIYGANYKHL KAIEGGSYEW FIKPGDIFKA GDVIGQYIQT STMQKKDLTL PYGGVVISIH NTGAVCQGSQ LMNLAII
NT Sequence
ATGCAAGCTA GTATGCATGC TTCAGAATTA CAGGGCAATG CTGTGATGAT CGAGCTTTTG GAATATTTCA AAAAGTATCA ACCGAAAGGC GATGTTTATC TAATCCCTCA ATGTAACCCT ATCGGTAAAG ATGGATTTAT AGGAGCAGGG CATCAGGGTA GATTTGACTC AGCAACAGGA GATAACTTCA ATAGATATTA CTTCTATCCT AAAATTGACT ATTCAGCATT TGTTAAAGAA CATATTAATT CAACTACAGC AGAGTATAAG CAAGCTTTTG AAAATCTTTT ACAACAAGAA TTAGATAAAG AACTATCTAA TGAATGGGAG CTATCTAGAG CAAAAAGATT AAATCTTCTA GTCCAAAGAG AAGCTCAGAA GGCAGATTAT GTGCTTGATT TACATACAGA TACAGATGCT ACTACATATT TATATACTGC AGATTTTGCA AGACAAACAG CAGAAAAATT TGGCTATAAA GATATGTTAA TTATAGATAA TGATAAAGCT AGTGGTGCTC TTGATGAGGC TATATTTTGT CCATGGTGGC ATTTAGCTCA AGAATTCAAA AAGCAGGATA GGGATGAAAA AGTACTAAAA GAAGGCTATA CACTCGAATT ATGCTCCGAG GAGTATATAA ATTTTGCTAA TGCTAAAATG CAAACTCAAG GCATCCTTAA TTATCTGAAA CACAAAGATA TTATTGATTC ACCTTTTGAT ACTGATTTAT TAAATACTGA TATTAGACAT CATATCTATG GAGCTAATTA TAAACATCTA AAAGCAATAG AGGGTGGCTC GTATGAGTGG TTTATTAAAC CGGGCGATAT TTTCAAAGCA GGCGATGTAA TTGGTCAATA TATCCAAACA TCTACTATGC AAAAGAAAGA TCTAACTCTG CCTTATGGCG GTGTTGTAAT TAGTATTCAT AATACTGGTG CAGTCTGTCA AGGAAGCCAG TTAATGAATC TAGCTATAAT A
Details for FrtuB.17360.a.B1.GE39266
HARVESTED ON: 6/10/2015
SEQUENCED ON: 6/5/2015
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMQ ASMHASELQG NAVMIELLEY FKKYQPKGDV YLIPQCNPIG KDGFIGAGHQ GRFDSATGDN FNRYYFYPKI DYSAFVKEHI NSTTAEYKQA FENLLQQELD KELSNEWELS RAKRLNLLVQ REAQKADYVL DLHTDTDATT YLYTADFARQ TAEKFGYKDM LIIDNDKASG ALDEAIFCPW WHLAQEFKKQ DRDEKVLKEG YTLELCSEEY INFANAKMQT QGILNYLKHK DIIDSPFDTD LLNTDIRHHI YGANYKHLKA IEGGSYEWFI KPGDIFKAGD VIGQYIQTST MQKKDLTLPY GGVVISIHNT GAVCQG
Validated NT Sequence
cttccttgac agactgcacc agtattatga atactaatta caacaccgcc ataaggcaga gttagatctt tcttttgcat agtagatgtt tggatatatt gaccaattac atcgcctgct ttgaaaatat cgcccggttt aataaaccac tcatacgagc caccctctat tgcttttaga tgtttataat tagctccata gatatgatgt ctaatatcag tatttaataa atcagtatca aaaggtgaat caataatatc tttgtgtttc agataattaa ggatgccttg agtttgcatt ttagcattag caaaatttat atactcctcg gagcataatt cgagtgtata gccttctttt agtacttttt catccctatc ctgctttttg aattcttgag ctaaatgcca ccatggacaa aatatagcct catcaagagc accactagct ttatcattat ctataattaa catatcttta tagccaaatt tttctgctgt ttgtcttgca aaatctgcag tatataaata tgtagtagca tctgtatctg tatgtaaatc aagcacataa tctgccttct gagcttctct ttggactaga agatttaatc tttttgctct agatagctcc cattcattag atagttcttt atctaattct tgttgtaaaa gattttcaaa agcttgctta tactctgctg tagttgaatt aatatgttct ttaacaaatg ctgaatagtc aattttagga tagaagtaat atctattgaa gttatctcct gttgctgagt caaatctacc ctgatgccct gctcctataa atccatcttt accgataggg ttacattgag ggattagata aacatcgcct ttcggttgat actttttgaa atattccaaa agctcgatca tcacagcatt gccctgtaat tctgaagcat gcatactagc ttgcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMQ ASMHASELQG NAVMIELLEY FKKYQPKGDV YLIPQCNPIG KDGFIGAGHQ GRFDSATGDN FNRYYFYPKI DYSAFVKEHI NSTTAEYKQA FENLLQQELD KELSNEWELS RAKRLNLLVQ REAQKADYVL DLHTDTDATT YLYTADFARQ TAEKFGYKDM LIIDNDKASG ALDEAIFCPW WHLAQEFKKQ DRDEKVLKEG YTLELCSEEY INFANAKMQT QGILNYLKHK DIIDSPFDTD LLNTDIRHHI YGANYKHLKA IEGGSYEWFI KPGDIFKAGD VIGQYIQTST MQKKDLTLPY GGVVISIHNT GAVCQGSQLM NLAII
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaag ctagtatgca tgcttcagaa ttacagggca atgctgtgat gatcgagctt ttggaatatt tcaaaaagta tcaaccgaaa ggcgatgttt atctaatccc tcaatgtaac cctatcggta aagatggatt tataggagca gggcatcagg gtagatttga ctcagcaaca ggagataact tcaatagata ttacttctat cctaaaattg actattcagc atttgttaaa gaacatatta attcaactac agcagagtat aagcaagctt ttgaaaatct tttacaacaa gaattagata aagaactatc taatgaatgg gagctatcta gagcaaaaag attaaatctt ctagtccaaa gagaagctca gaaggcagat tatgtgcttg atttacatac agatacagat gctactacat atttatatac tgcagatttt gcaagacaaa cagcagaaaa atttggctat aaagatatgt taattataga taatgataaa gctagtggtg ctcttgatga ggctatattt tgtccatggt ggcatttagc tcaagaattc aaaaagcagg atagggatga aaaagtacta aaagaaggct atacactcga attatgctcc gaggagtata taaattttgc taatgctaaa atgcaaactc aaggcatcct taattatctg aaacacaaag atattattga ttcacctttt gatactgatt tattaaatac tgatattaga catcatatct atggagctaa ttataaacat ctaaaagcaa tagagggtgg ctcgtatgag tggtttatta aaccgggcga tattttcaaa gcaggcgatg taattggtca atatatccaa acatctacta tgcaaaagaa agatctaact ctgccttatg gcggtgttgt aattagtatt cataatactg gtgcagtctg tcaaggaagc cagttaatga atctagctat aatatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat