SSGCID: Target Page

FrtuB.00130.a

Peptidyl-prolyl cis-trans isomerase

CENTER ID:	FrtuB.00130.a
ORGANISM:	Francisella tularensis subsp. tularensis SCHU S4 / Schu 4
ASSOCIATED DISEASE:	tularemia
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIA

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	AA START	AA STOP
FrtuB.00130.a.B1.GE37184	full length	1	270
FrtuB.00130.a.HC2.GE39570	Craig soluble domain 1	23	270
FrtuB.00130.a.HC21.GE40007	Craig soluble domain 1	23	270
FrtuB.00130.a.HC3.GE39571	Craig soluble domain 2	61	270
FrtuB.00130.a.HW2.GE40008	Craig soluble domain 1	23	270
FrtuB.00130.a.HW3.GE40011	Craig soluble domain 2	61	270

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	AA START	AA STOP
FrtuB.00130.a.HW2.PW37892	Craig soluble domain 1	23	270
FrtuB.00130.a.HW2.PW37893	Craig soluble domain 1	23	270
FrtuB.00130.a.HW3.PW37894	Craig soluble domain 2	61	270
FrtuB.00130.a.HX3.PW37895	Craig soluble domain 2	61	270
FrtuB.00130.a.HC21.PW37896	Craig soluble domain 1	23	270
FrtuB.00130.a.HC21.PW37897	Craig soluble domain 1	23	270

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|177416.36.peg.1802
RefSeq:	YP_170026.1
UniProt:	Q5NG16

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKLKKIVAVI SCSLLGLAMS SCSTNTDSVD STNAQQVTKN TEAKGVATNK SAQDNPTIKM GANASYVVGY QVGAGIAKQD FGLYDKQTIA GFADAINGNK PRISESQIRR NMETLKDKMI KKQLDTANLN KTKSQEFMAQ IAKMDNAIKV DDGVYYQMIK QGDGKNPKSD SQVTIAYKGT TPVIAYEDDK SKLNEVKEAK LIGPTFDSSD SATFPLRNLI ECWKDAIPQI PNGSTIILYC SPDKAYGTRA PAVIGPNQAL SFEITLKDFK

NT Sequence

atgaagttaa agaaaatagt agcagttata tcatgctcat tattaggact agctatgagt tcatgttcaa caaatacaga ttccgtagat tctacgaatg ctcaacaagt aactaaaaat actgaagcta aaggtgttgc tacaaacaaa tcagctcaag ataatccaac aataaaaatg ggagctaatg caagctacgt agtaggctat caagtaggtg ctggtatagc taagcaagat tttggcttat acgataagca aacaatagcc ggttttgcag atgctatcaa tggtaataag ccaagaatct ctgagagtca aatccgcaga aatatggaaa ctcttaaaga taagatgatt aaaaaacaac tagatacagc taatttaaat aaaacaaaat cacaagaatt tatggcacaa attgctaaaa tggataatgc tataaaagtt gatgatggtg tttactatca aatgattaag caaggcgatg gcaaaaatcc taaatctgat agtcaagtta caatagctta taaaggaact acgccagtta tagcttacga agatgataaa tcgaaactaa acgaggtcaa agaagctaaa ctaattggtc ctacatttga ctcgagtgat agcgcaacat tcccactcag aaacctaatc gaatgttgga aagatgctat tcctcaaata cctaatggat caacaataat attatattgc tctccagata aggcatatgg aactagagcg cccgctgtca taggacctaa tcaagcactt tcttttgaaa ttacgcttaa agattttaaa taa

Details for FrtuB.00130.a.B1.GE37184

HARVESTED ON:	8/13/2013
SEQUENCED ON:	10/23/2013
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK LKKIVAVISC SLLGLAMSSC STNTDSVDST NAQQVTKNTE AKGVATNKSA QDNPTIKMGA NASYVVGYQV GAGIAKQDFG LYDKQTIAGF ADAINGNKPR ISESQIRRNM ETLKDKMIKK QLDTANLNKT KSQEFMAQIA KMDNAIKVDD GVYYQMIKQG DGKNPKSDSQ VTIAYKGTTP VIAYEDDKSK LNEVKEAKLI GPTFDSSDSA TFPLRNLIEC WKDAIPQIPN GSTIILYCSP DKAYGTRAPA VIGPNQALSF EITLKNFK

Validated NT Sequence

atcttactca cttatttaaa atttttaagc gtaatttcaa aagaaagtgc ttgattaggt cctatgacag cgggcgctct agttccatat gccttatctg gagagcaata taatattatt gttgatccat taggtatttg aggaatagca tctttccaac attcgattag gtttctgagt gggaatgttg cgctatcact cgagtcaaat gtaggaccaa ttagtttagc ttctttgacc tcgtttagtt tcgatttatc atcttcgtaa gctataactg gcgtagttcc tttataagct attgtaactt gactatcaga tttaggattt ttgccatcgc cttgcttaat catttgatag taaacaccat catcaacttt tatagcatta tccattttag caatttgtgc cataaattct tgtgattttg ttttatttaa attagctgta tctagttgtt ttttaatcat cttatcttta agagtttcca tatttctgcg gatttgactc tcagagattc ttggcttatt accattgata gcatctgcaa aaccggctat tgtttgctta tcgtataagc caaaatcttg cttagctata ccagcaccta cttgatagcc tactacgtag cttgcattag ctcccatttt tattgttgga ttatcttgag ctgatttgtt tgtagcaaca cctttagctt cagtattttt agttacttgt tgagcattcg tagaatctac ggaatctgta tttgttgaac atgaactcat agctagtcct aataatgagc atgatataac tgctactatt ttctttaact tcatatggtg gtggtggtgg tgagccatgg tatatctcct tcttaaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagt taaagaaaat agtagcagtt atatcatgct cattattagg actagctatg agttcatgtt caacaaatac agattccgta gattctacga atgctcaaca agtaactaaa aatactgaag ctaaaggtgt tgctacaaac aaatcagctc aagataatcc aacaataaaa atgggagcta atgcaagcta cgtagtaggc tatcaagtag gtgctggtat agctaagcaa gattttggct tatacgataa gcaaacaata gccggttttg cagatgctat caatggtaat aagccaagaa tctctgagag tcaaatccgc agaaatatgg aaactcttaa agataagatg attaaaaaac aactagatac agctaattta aataaaacaa aatcacaaga atttatggca caaattgcta aaatggataa tgctataaaa gttgatgatg gtgtttacta tcaaatgatt aagcaaggcg atggcaaaaa tcctaaatct gatagtcaag ttacaatagc ttataaagga actacgccag ttatagctta cgaagatgat aaatcgaaac taaacgaggt caaagaagct aaactaattg gtcctacatt tgactcgagt gatagcgcaa cattcccact cagaaaccta atcgaatgtt ggaaagatgc tattcctcaa atacctaatg gatcaacaat aatattatat tgctctccag ataaggcata tggaactaga gcgcccgctg tcataggacc taatcaagca ctttcttttg aaattacgct taaagatttt aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for FrtuB.00130.a.HC2.GE39570

HARVESTED ON:	data unavailable
SEQUENCED ON:	data unavailable
EXPECTED MW:	67kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDA ALAAAQTNST NTDSVDSTNA QQVTKNTEAK GVATNKSAQD NPTIKMGANA SYVVGYQVGA GIAKQDFGLY DKQTIAGFAD AINGNKPRIS ESQIRRNMET LKDKMIKKQL DTANLNKTKS QEFMAQIAKM DNAIKVDDGV YYQMIKQGDG KNPKSDSQVT IAYKGTTPVI AYEDDKSKLN EVKEAKLIGP TFDSSDSATF PLRNLIECWK DAIPQIPNGS TIILYCSPDK AYGTRAPAVI GPNQALSFEI TLKDFK

Full NT Sequence (Expression Vector + Insert)

ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga gcacttcacc aacaaggacc atagcatatg aaaatcgaag aaggtaaact ggtaatctgg attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc gccagcggtc gtcagactgt cgatgcagcc ctggcagccg cgcagactaa ttcaacaaat acagattccg tagattctac gaatgctcaa caagtaacta aaaatactga agctaaaggt gttgctacaa acaaatcagc tcaagataat ccaacaataa aaatgggagc taatgcaagc tacgtagtag gctatcaagt aggtgctggt atagctaagc aagattttgg cttatacgat aagcaaacaa tagccggttt tgcagatgct atcaatggta ataagccaag aatctctgag agtcaaatcc gcagaaatat ggaaactctt aaagataaga tgattaaaaa acaactagat acagctaatt taaataaaac aaaatcacaa gaatttatgg cacaaattgc taaaatggat aatgctataa aagttgatga tggtgtttac tatcaaatga ttaagcaagg cgatggcaaa aatcctaaat ctgatagtca agttacaata gcttataaag gaactacgcc agttatagct tacgaagatg ataaatcgaa actaaacgag gtcaaagaag ctaaactaat tggtcctaca tttgactcga gtgatagcgc aacattccca ctcagaaacc taatcgaatg ttggaaagat gctattcctc aaatacctaa tggatcaaca ataatattat attgctctcc agataaggca tatggaacta gagcgcccgc tgtcatagga cctaatcaag cactttcttt tgaaattacg cttaaagatt ttaaagcggc cgcagctagc gaattcggat cctctagagt cgacctgcag gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggca gcttggctgt tttggcggat gagataagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg tttcccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgcagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgaaat t

Details for FrtuB.00130.a.HC21.GE40007

HARVESTED ON:	2/25/2016
SEQUENCED ON:	1/5/2016
EXPECTED MW:	67kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	94
PERCENT COVERAGE:	44

Validated AA Sequence

MNADTDYSIA EAAFNKGETA MTINGPWAWS NIDTSKVNYG VTVLPTFKGQ PSKPFVGVLS AGINAASPNK ELAKEFLENY LLTDEGLEAV NKDKPLGAVA LKSYEEELAK DPRIAATMEN AQKGEIMPNI PQMSAFWYAV RTAVINAASG RQTVDAALAA AQTNSTNTDS VDSTNAQQVT KNTEAKGVAT NKSAQDNPTI KMGANASYVV GYQVGAGIAK QDFGLYDKQT IAGFAXAING NKPRXXXXQX RXNMETLKXX MXXXQLXXXN LNK

Validated NT Sequence

ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgcag ccctggcagc cgcgcagact aattcaacaa atacagattc cgtagattct acgaatgctc aacaagtaac taaaaatact gaagctaaag gtgttgctac aaacaaatca gctcaagata atccaacaat aaaaatggga gctaatgcaa gctacgtagt aggctatcaa gtaggtgctg gtatagctaa gcaagatttt ggcttatacg ataagcaaac aatagccggt tttgcnnntg ctatcaatgg taataagcca agaatnnctn nnnnncaann ncgnnnnaat atggaaactc ttaaagnnnn gatgnntnnn nnncaactan atannnctaa tttaaataaa ac

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

Details for FrtuB.00130.a.HC3.GE39571

HARVESTED ON:	data unavailable
SEQUENCED ON:	10/23/2015
EXPECTED MW:	63kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDA ALAAAQTNGA NASYVVGYQV GAGIAKQDFG LYDKQTIAGF ADAINGNKPR ISESQIRRNM ETLKDKMIKK QLDTANLNKT KSQEFMAQIA KMDNAIKVDD GVYYQMIKQG DGKNPKSDSQ VTIAYKGTTP VIAYEDDKSK LNEVKEAKLI GPTFDSSDSA TFPLRNLIEC WKDAIPQIPN GSTIILYCSP DKAYGTRAPA VIGPNQALSF EITLKDFK

Full NT Sequence (Expression Vector + Insert)

ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga gcacttcacc aacaaggacc atagcatatg aaaatcgaag aaggtaaact ggtaatctgg attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc gccagcggtc gtcagactgt cgatgcagcc ctggcagccg cgcagactaa tggagctaat gcaagctacg tagtaggcta tcaagtaggt gctggtatag ctaagcaaga ttttggctta tacgataagc aaacaatagc cggttttgca gatgctatca atggtaataa gccaagaatc tctgagagtc aaatccgcag aaatatggaa actcttaaag ataagatgat taaaaaacaa ctagatacag ctaatttaaa taaaacaaaa tcacaagaat ttatggcaca aattgctaaa atggataatg ctataaaagt tgatgatggt gtttactatc aaatgattaa gcaaggcgat ggcaaaaatc ctaaatctga tagtcaagtt acaatagctt ataaaggaac tacgccagtt atagcttacg aagatgataa atcgaaacta aacgaggtca aagaagctaa actaattggt cctacatttg actcgagtga tagcgcaaca ttcccactca gaaacctaat cgaatgttgg aaagatgcta ttcctcaaat acctaatgga tcaacaataa tattatattg ctctccagat aaggcatatg gaactagagc gcccgctgtc ataggaccta atcaagcact ttcttttgaa attacgctta aagattttaa agcggccgca gctagcgaat tcggatcctc tagagtcgac ctgcaggcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcagctt ggctgttttg gcggatgaga taagattttc agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttc ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt accccggttg ataatcagaa aagccccaaa aacaggaaga ttgtataagc aaatatttaa attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcacccaaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gcagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgaaatt

Details for FrtuB.00130.a.HW2.GE40008

HARVESTED ON:	2/25/2016
SEQUENCED ON:	1/13/2016
EXPECTED MW:	41kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	91
PERCENT COVERAGE:	88

Validated AA Sequence

MXXDKIIHLT DXXFDTDVLK ADGAILVDFW AEWCGPCKMI APILDEIADE YQGKLTVAKL NIDQNPGTAP KYGIRGIPTL LLFKNGEVAA TKVGALSKGQ LKEFLDANLA GTLEAQTQGP GSMSTNTDSV DSTNAQQVTK NTEAKGVATN KSAQDNPTIK MGANASYVVG YQVGAGIAKQ DFGLYDKQTI AGFADAINGN KPRISESQIR RNMETLKDKM IKKQLDTANL NKTKSQEFMA QIAKMDNAIK VDDGVYYQMI KQGDGKNPKS DSQVTIAYKG TTPVIAYEDD KSKLNEVKEA KLIGPXFDXX XXXXXPLXXX XEXXXXXXXX XXXXXX

Validated NT Sequence

atgangngcg ataaaattat tcacctgact gacgannntt ttgacacgga tgtactcaaa gcggacgggg cgatcctcgt cgatttctgg gcagagtggt gcggtccgtg caaaatgatc gccccgattc tggatgaaat cgctgacgaa tatcagggca aactgaccgt tgcaaaactg aacatcgatc aaaaccctgg cactgcgccg aaatatggca tccgtggtat cccgactctg ctgctgttca aaaacggtga agtggcggca accaaagtgg gtgcactgtc taaaggtcag ttgaaagagt tcctcgacgc taacctggcc ggtaccctgg aagctcagac ccagggtcct ggttcgatgt caacaaatac agattccgta gattctacga atgctcaaca agtaactaaa aatactgaag ctaaaggtgt tgctacaaac aaatcagctc aagataatcc aacaataaaa atgggagcta atgcaagcta cgtagtaggc tatcaagtag gtgctggtat agctaagcaa gattttggct tatacgataa gcaaacaata gccggttttg cagatgctat caatggtaat aagccaagaa tctctgagag tcaaatccgc agaaatatgg aaactcttaa agataagatg attaaaaaac aactagatac agctaattta aataaaacaa aatcacaaga atttatggca caaattgcta aaatggataa tgctataaaa gttgatgatg gtgtttacta tcaaatgatt aagcaaggcg atggcaaaaa tcctaaatct gatagtcaag ttacaatagc ttataaagga actacgccag ttatagctta cgaagatgat aaatcgaaac taaacgaggt caaagaagct aaactaattg gtcctanatt tgactnnngt gnnnnnnnnn nnntcccnct nnnnnnncnn nnngaannnn nnnannnnnc tannnnnnnn nnnntannnn nnnnnnnata atannnnnnt g

Expected Protein Sequence

MAHHHHHHMK SDKIIHLTDD SFDTDVLKAD GAILVDFWAE WCGPCKMIAP ILDEIADEYQ GKLTVAKLNI DQNPGTAPKY GIRGIPTLLL FKNGEVAATK VGALSKGQLK EFLDANLAGT LEAQTQGPGS STNTDSVDST NAQQVTKNTE AKGVATNKSA QDNPTIKMGA NASYVVGYQV GAGIAKQDFG LYDKQTIAGF ADAINGNKPR ISESQIRRNM ETLKDKMIKK QLDTANLNKT KSQEFMAQIA KMDNAIKVDD GVYYQMIKQG DGKNPKSDSQ VTIAYKGTTP VIAYEDDKSK LNEVKEAKLI GPTFDSSDSA TFPLRNLIEC WKDAIPQIPN GSTIILYCSP DKAYGTRAPA VIGPNQALSF EITLKDFK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagagcga taaaattatt cacctgactg acgacagttt tgacacggat gtactcaaag cggacggggc gatcctcgtc gatttctggg cagagtggtg cggtccgtgc aaaatgatcg ccccgattct ggatgaaatc gctgacgaat atcagggcaa actgaccgtt gcaaaactga acatcgatca aaaccctggc actgcgccga aatatggcat ccgtggtatc ccgactctgc tgctgttcaa aaacggtgaa gtggcggcaa ccaaagtggg tgcactgtct aaaggtcagt tgaaagagtt cctcgacgct aacctggccg gtaccctgga agctcagacc cagggtcctg gttcgtcaac aaatacagat tccgtagatt ctacgaatgc tcaacaagta actaaaaata ctgaagctaa aggtgttgct acaaacaaat cagctcaaga taatccaaca ataaaaatgg gagctaatgc aagctacgta gtaggctatc aagtaggtgc tggtatagct aagcaagatt ttggcttata cgataagcaa acaatagccg gttttgcaga tgctatcaat ggtaataagc caagaatctc tgagagtcaa atccgcagaa atatggaaac tcttaaagat aagatgatta aaaaacaact agatacagct aatttaaata aaacaaaatc acaagaattt atggcacaaa ttgctaaaat ggataatgct ataaaagttg atgatggtgt ttactatcaa atgattaagc aaggcgatgg caaaaatcct aaatctgata gtcaagttac aatagcttat aaaggaacta cgccagttat agcttacgaa gatgataaat cgaaactaaa cgaggtcaaa gaagctaaac taattggtcc tacatttgac tcgagtgata gcgcaacatt cccactcaga aacctaatcg aatgttggaa agatgctatt cctcaaatac ctaatggatc aacaataata ttatattgct ctccagataa ggcatatgga actagagcgc ccgctgtcat aggacctaat caagcacttt cttttgaaat tacgcttaaa gattttaaaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for FrtuB.00130.a.HW3.GE40011

HARVESTED ON:	2/25/2016
SEQUENCED ON:	1/13/2016
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	62

Validated AA Sequence

MGANASYVVG YQVGAGIAKQ DFGLYDKQTI AGFADAINGN KPRISESQIR RNMETLKDKM IKKQLDTANL NKTKSQEFMA QIAKMDNAIK VDDGVYYQMI KQGDGKNPKS DSQVTIAYKG TTPVIAYEDD KSKLNEVKEA KLIGPTFDSS DSATFPLRNL IECWKDAIPQ IPNGSTIILY CSPDKAYGTR APAVIGPNQA LSFEITLKDF K

Validated NT Sequence

ctgcgccgaa anatggcatc cgtggtatcc cgactctgct gctgtnnana nnnnntgaag tggcggcaac caaagtgggt gcactgtcta aaggtcagtt gaaagagttc ctcgacgcta acctggccgg taccctggaa gctcagaccc agggtcctgg ttcgatggga gctaatgcaa gctacgtagt aggctatcaa gtaggtgctg gtatagctaa gcaagatttt ggcttatacg ataagcaaac aatagccggt tttgcagatg ctatcaatgg taataagcca agaatctctg agagtcaaat ccgcagaaat atggaaactc ttaaagataa gatgattaaa aaacaactag atacagctaa tttaaataaa acaaaatcac aagaatttat ggcacaaatt gctaaaatgg ataatgctat aaaagttgat gatggtgttt actatcaaat gattaagcaa ggcgatggca aaaatcctaa atctgatagt caagttacaa tagcttataa aggaactacg ccagttatag cttacgaaga tgataaatcg aaactaaacg aggtcaaaga agctaaacta attggtccta catttgactc gagtgatagc gcaacattcc cactcagaaa cctaatcgaa tgttggaaag atgctattcc tcaaatacct aatggatcaa caataatatt atattgctct ccagataagg catatggaac tagagcgccc gctgtcatag gacctaatca agcactttct tttgaaatta cgcttaaaga ttttaaataa taa

Expected Protein Sequence

MAHHHHHHMK SDKIIHLTDD SFDTDVLKAD GAILVDFWAE WCGPCKMIAP ILDEIADEYQ GKLTVAKLNI DQNPGTAPKY GIRGIPTLLL FKNGEVAATK VGALSKGQLK EFLDANLAGT LEAQTQGPGS GANASYVVGY QVGAGIAKQD FGLYDKQTIA GFADAINGNK PRISESQIRR NMETLKDKMI KKQLDTANLN KTKSQEFMAQ IAKMDNAIKV DDGVYYQMIK QGDGKNPKSD SQVTIAYKGT TPVIAYEDDK SKLNEVKEAK LIGPTFDSSD SATFPLRNLI ECWKDAIPQI PNGSTIILYC SPDKAYGTRA PAVIGPNQAL SFEITLKDFK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagagcga taaaattatt cacctgactg acgacagttt tgacacggat gtactcaaag cggacggggc gatcctcgtc gatttctggg cagagtggtg cggtccgtgc aaaatgatcg ccccgattct ggatgaaatc gctgacgaat atcagggcaa actgaccgtt gcaaaactga acatcgatca aaaccctggc actgcgccga aatatggcat ccgtggtatc ccgactctgc tgctgttcaa aaacggtgaa gtggcggcaa ccaaagtggg tgcactgtct aaaggtcagt tgaaagagtt cctcgacgct aacctggccg gtaccctgga agctcagacc cagggtcctg gttcgggagc taatgcaagc tacgtagtag gctatcaagt aggtgctggt atagctaagc aagattttgg cttatacgat aagcaaacaa tagccggttt tgcagatgct atcaatggta ataagccaag aatctctgag agtcaaatcc gcagaaatat ggaaactctt aaagataaga tgattaaaaa acaactagat acagctaatt taaataaaac aaaatcacaa gaatttatgg cacaaattgc taaaatggat aatgctataa aagttgatga tggtgtttac tatcaaatga ttaagcaagg cgatggcaaa aatcctaaat ctgatagtca agttacaata gcttataaag gaactacgcc agttatagct tacgaagatg ataaatcgaa actaaacgag gtcaaagaag ctaaactaat tggtcctaca tttgactcga gtgatagcgc aacattccca ctcagaaacc taatcgaatg ttggaaagat gctattcctc aaatacctaa tggatcaaca ataatattat attgctctcc agataaggca tatggaacta gagcgcccgc tgtcatagga cctaatcaag cactttcttt tgaaattacg cttaaagatt ttaaaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for FrtuB.00130.a.HW2.PW37892

PURIFICATION DATe:	4/11/2016
CONCENTRATION:	13mg/ml
OBSERVED MW:	40kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	100µl
PERCENT IDENTITY:	91
PERCENT COVERAGE:	88

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGAAGAGCGA TAAAATTATT CACCTGACTG ACGACAGTTT TGACACGGAT GTACTCAAAG CGGACGGGGC GATCCTCGTC GATTTCTGGG CAGAGTGGTG CGGTCCGTGC AAAATGATCG CCCCGATTCT GGATGAAATC GCTGACGAAT ATCAGGGCAA ACTGACCGTT GCAAAACTGA ACATCGATCA AAACCCTGGC ACTGCGCCGA AATATGGCAT CCGTGGTATC CCGACTCTGC TGCTGTTCAA AAACGGTGAA GTGGCGGCAA CCAAAGTGGG TGCACTGTCT AAAGGTCAGT TGAAAGAGTT CCTCGACGCT AACCTGGCCG GTACCCTGGA AGCTCAGACC CAGGGTCCTG GTTCGTCAAC AAATACAGAT TCCGTAGATT CTACGAATGC TCAACAAGTA ACTAAAAATA CTGAAGCTAA AGGTGTTGCT ACAAACAAAT CAGCTCAAGA TAATCCAACA ATAAAAATGG GAGCTAATGC AAGCTACGTA GTAGGCTATC AAGTAGGTGC TGGTATAGCT AAGCAAGATT TTGGCTTATA CGATAAGCAA ACAATAGCCG GTTTTGCAGA TGCTATCAAT GGTAATAAGC CAAGAATCTC TGAGAGTCAA ATCCGCAGAA ATATGGAAAC TCTTAAAGAT AAGATGATTA AAAAACAACT AGATACAGCT AATTTAAATA AAACAAAATC ACAAGAATTT ATGGCACAAA TTGCTAAAAT GGATAATGCT ATAAAAGTTG ATGATGGTGT TTACTATCAA ATGATTAAGC AAGGCGATGG CAAAAATCCT AAATCTGATA GTCAAGTTAC AATAGCTTAT AAAGGAACTA CGCCAGTTAT AGCTTACGAA GATGATAAAT CGAAACTAAA CGAGGTCAAA GAAGCTAAAC TAATTGGTCC TACATTTGAC TCGAGTGATA GCGCAACATT CCCACTCAGA AACCTAATCG AATGTTGGAA AGATGCTATT CCTCAAATAC CTAATGGATC AACAATAATA TTATATTGCT CTCCAGATAA GGCATATGGA ACTAGAGCGC CCGCTGTCAT AGGACCTAAT CAAGCACTTT CTTTTGAAAT TACGCTTAAA GATTTTAAAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A

Details for FrtuB.00130.a.HW2.PW37893

PURIFICATION DATe:	4/11/2016
CONCENTRATION:	21.5mg/ml
OBSERVED MW:	40kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	91
PERCENT COVERAGE:	88

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

Details for FrtuB.00130.a.HW3.PW37894

PURIFICATION DATe:	4/11/2016
CONCENTRATION:	21.5mg/ml
OBSERVED MW:	38kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	62

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGAAGAGCGA TAAAATTATT CACCTGACTG ACGACAGTTT TGACACGGAT GTACTCAAAG CGGACGGGGC GATCCTCGTC GATTTCTGGG CAGAGTGGTG CGGTCCGTGC AAAATGATCG CCCCGATTCT GGATGAAATC GCTGACGAAT ATCAGGGCAA ACTGACCGTT GCAAAACTGA ACATCGATCA AAACCCTGGC ACTGCGCCGA AATATGGCAT CCGTGGTATC CCGACTCTGC TGCTGTTCAA AAACGGTGAA GTGGCGGCAA CCAAAGTGGG TGCACTGTCT AAAGGTCAGT TGAAAGAGTT CCTCGACGCT AACCTGGCCG GTACCCTGGA AGCTCAGACC CAGGGTCCTG GTTCGGGAGC TAATGCAAGC TACGTAGTAG GCTATCAAGT AGGTGCTGGT ATAGCTAAGC AAGATTTTGG CTTATACGAT AAGCAAACAA TAGCCGGTTT TGCAGATGCT ATCAATGGTA ATAAGCCAAG AATCTCTGAG AGTCAAATCC GCAGAAATAT GGAAACTCTT AAAGATAAGA TGATTAAAAA ACAACTAGAT ACAGCTAATT TAAATAAAAC AAAATCACAA GAATTTATGG CACAAATTGC TAAAATGGAT AATGCTATAA AAGTTGATGA TGGTGTTTAC TATCAAATGA TTAAGCAAGG CGATGGCAAA AATCCTAAAT CTGATAGTCA AGTTACAATA GCTTATAAAG GAACTACGCC AGTTATAGCT TACGAAGATG ATAAATCGAA ACTAAACGAG GTCAAAGAAG CTAAACTAAT TGGTCCTACA TTTGACTCGA GTGATAGCGC AACATTCCCA CTCAGAAACC TAATCGAATG TTGGAAAGAT GCTATTCCTC AAATACCTAA TGGATCAACA ATAATATTAT ATTGCTCTCC AGATAAGGCA TATGGAACTA GAGCGCCCGC TGTCATAGGA CCTAATCAAG CACTTTCTTT TGAAATTACG CTTAAAGATT TTAAAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA

Details for FrtuB.00130.a.HX3.PW37895

PURIFICATION DATe:	4/11/2016
CONCENTRATION:	7mg/ml
OBSERVED MW:	81kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	32

Protocol Notes

notes unavailable

Validated AA Sequence

MAARNICWFG DEAGTLEAQT QGPGSMGANA SYVVGYQVGA GIAKQDFGLY DKQTIAGFAD AINGNKPRIS ESQIRRNMET LKDKMIKKQL DTANLNKTKS QEFMAQIAKM DNAIKVDDGV YYQMIKQGDG KNPKSDSQVT IAYKGTTPVI AYEDDKSKLN EVKEAKLIGP TFDSSDSATF PLRNLIECWK DAIPQIPNGS TIILYCSPDK AYGTKAPAVI GPNQALSFEI TLKDFK

Validated NT Sequence

tgagcactgc gcgagcgtgc taaaaatgca ctggccacca ttgcacaggc cnnnnaagaa agcctcggtg ataacaaacc ggctgacgat ctgctgaacc ttgaaggggt agatcgtgat ttggcattca aactggccgc ccgtggcgtt tgtacgctgg aagatctcgc cgaacagggc attgatgatc tggctgatat cgaagggttg accgacgaaa aagccggagc actgattatg gctgcccgta acatttgctg gttcggtgac gaagcgggta ccctggaagc tcagacccag ggtcctggtt cgatgggagc taatgcaagc tacgtagtag gctatcaagt aggtgctggt atagctaagc aagattttgg cttatacgat aagcaaacaa tagccggttt tgcagatgct atcaatggta ataagccaag aatctctgag agtcaaatcc gcagaaatat ggaaactctt aaagataaga tgattaaaaa acaactagat acagctaatt taaataaaac aaaatcacaa gaatttatgg cacaaattgc taaaatggat aatgctataa aagttgatga tggtgtttac tatcaaatga ttaagcaagg cgatggcaaa aatcctaaat ctgatagtca agttacaata gcttataaag gaactacgcc agttatagct tacgaagatg ataaatcgaa actaaacgag gtcaaagaag ctaaactaat tggtcctaca tttgactcga gtgatagcgc aacattccca ctcagaaacc taatcgaatg ttggaaagat gctattcctc aaatacctaa tggatcaaca ataatattat attgctctcc agataaggca tatggaacta aagcgcccgc tgtcatagga cctaatcaag cactttcttt tgaaattacg cttaaagatt ttaaataata

Expressed Protein Sequence

MAHHHHHHMK NKEILAVVEA VSNEKALPRE KIFEALESAL ATATKKKYEQ EIDVRVQIDR KSGDFDTFRR WLVVDEVTQP TKEITLEAAR YEDESLNLGD YVEDQIESVT FDRITTQTAK QVIVQKVREA ERAMVVDQFR EHEGEIITGV VKKVNRDNIS LDLGNNAEAV ILREDMLPRE NFRPGDRVRG VLYSVRPEAR GAQLFVTRSK PEMLIELFRI EVPEIGEEVI EIKAAARDPG SRAKIAVKTN DKRIDPVGAC VGMRGARVQA VSTELGGERI DIVLWDDNPA QFVINAMAPA DVASIVVDED KHTMDIAVEA GNLAQAIGRN GQNVRLASQL SGWELNVMTV DDLQAKHQAE AHAAIDTFTK YLDIDEDFAT VLVEEGFSTL EELAYVPMKE LLEIEGLDEP TVEALRERAK NALATIAQAQ EESLGDNKPA DDLLNLEGVD RDLAFKLAAR GVCTLEDLAE QGIDDLADIE GLTDEKAGAL IMAARNICWF GDEAGTLEAQ TQGPGSGANA SYVVGYQVGA GIAKQDFGLY DKQTIAGFAD AINGNKPRIS ESQIRRNMET LKDKMIKKQL DTANLNKTKS QEFMAQIAKM DNAIKVDDGV YYQMIKQGDG KNPKSDSQVT IAYKGTTPVI AYEDDKSKLN EVKEAKLIGP TFDSSDSATF PLRNLIECWK DAIPQIPNGS TIILYCSPDK AYGTRAPAVI GPNQALSFEI TLKDFK

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGAAGAACAA AGAAATTTTG GCTGTAGTTG AAGCCGTATC CAATGAAAAG GCGCTACCTC GCGAGAAGAT TTTCGAAGCA TTGGAAAGCG CGCTGGCGAC AGCAACAAAG AAAAAATATG AACAAGAGAT CGACGTCCGC GTACAGATCG ATCGCAAAAG CGGTGATTTT GACACTTTCC GTCGCTGGTT AGTTGTTGAT GAAGTCACCC AGCCGACCAA GGAAATCACC CTTGAAGCCG CACGTTATGA AGATGAAAGC CTGAACCTGG GCGATTACGT TGAAGATCAG ATTGAGTCTG TTACCTTTGA CCGTATCACT ACCCAGACGG CAAAACAGGT TATCGTGCAG AAAGTGCGTG AAGCCGAACG TGCGATGGTG GTTGATCAGT TCCGTGAACA CGAAGGTGAA ATCATCACCG GCGTGGTGAA AAAAGTAAAC CGCGACAACA TCTCTCTGGA TCTGGGCAAC AACGCTGAAG CCGTGATCCT GCGCGAAGAT ATGCTGCCGC GTGAAAACTT CCGCCCTGGC GACCGCGTTC GTGGCGTGCT CTATTCCGTT CGCCCGGAAG CGCGTGGCGC GCAACTGTTC GTCACTCGTT CCAAGCCGGA AATGCTGATC GAACTGTTCC GTATTGAAGT GCCAGAAATC GGCGAAGAAG TGATTGAAAT TAAAGCAGCG GCTCGCGATC CGGGTTCTCG TGCGAAAATC GCGGTGAAAA CCAACGATAA ACGTATCGAT CCGGTAGGTG CTTGCGTAGG TATGCGTGGC GCGCGTGTTC AGGCGGTGTC TACTGAACTG GGTGGCGAGC GTATCGATAT CGTCCTGTGG GATGATAACC CGGCGCAGTT CGTGATTAAC GCAATGGCAC CGGCAGACGT TGCTTCTATC GTGGTGGATG AAGATAAACA CACCATGGAT ATCGCCGTTG AAGCCGGTAA CCTGGCGCAG GCGATTGGCC GTAACGGTCA GAACGTGCGT CTGGCTTCGC AGCTGAGCGG TTGGGAACTC AACGTGATGA CCGTTGACGA CCTGCAGGCT AAGCATCAGG CGGAAGCGCA CGCAGCGATC GACACCTTCA CCAAATATCT CGACATCGAC GAAGACTTCG CGACTGTTCT GGTAGAAGAA GGCTTCTCGA CGCTGGAAGA ATTGGCCTAT GTGCCGATGA AAGAGCTGTT GGAAATCGAA GGCCTTGATG AGCCGACCGT TGAAGCACTG CGCGAGCGTG CTAAAAATGC ACTGGCCACC ATTGCACAGG CCCAGGAAGA AAGCCTCGGT GATAACAAAC CGGCTGACGA TCTGCTGAAC CTTGAAGGGG TAGATCGTGA TTTGGCATTC AAACTGGCCG CCCGTGGCGT TTGTACGCTG GAAGATCTCG CCGAACAGGG CATTGATGAT CTGGCTGATA TCGAAGGGTT GACCGACGAA AAAGCCGGAG CACTGATTAT GGCTGCCCGT AACATTTGCT GGTTCGGTGA CGAAGCGGGT ACCCTGGAAG CTCAGACCCA GGGTCCTGGT TCGGGAGCTA ATGCAAGCTA CGTAGTAGGC TATCAAGTAG GTGCTGGTAT AGCTAAGCAA GATTTTGGCT TATACGATAA GCAAACAATA GCCGGTTTTG CAGATGCTAT CAATGGTAAT AAGCCAAGAA TCTCTGAGAG TCAAATCCGC AGAAATATGG AAACTCTTAA AGATAAGATG ATTAAAAAAC AACTAGATAC AGCTAATTTA AATAAAACAA AATCACAAGA ATTTATGGCA CAAATTGCTA AAATGGATAA TGCTATAAAA GTTGATGATG GTGTTTACTA TCAAATGATT AAGCAAGGCG ATGGCAAAAA TCCTAAATCT GATAGTCAAG TTACAATAGC TTATAAAGGA ACTACGCCAG TTATAGCTTA CGAAGATGAT AAATCGAAAC TAAACGAGGT CAAAGAAGCT AAACTAATTG GTCCTACATT TGACTCGAGT GATAGCGCAA CATTCCCACT CAGAAACCTA ATCGAATGTT GGAAAGATGC TATTCCTCAA ATACCTAATG GATCAACAAT AATATTATAT TGCTCTCCAG ATAAGGCATA TGGAACTAGA GCGCCCGCTG TCATAGGACC TAATCAAGCA CTTTCTTTTG AAATTACGCT TAAAGATTTT AAAAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA

Details for FrtuB.00130.a.HC21.PW37896

PURIFICATION DATe:	4/18/2016
CONCENTRATION:	15.7mg/ml
OBSERVED MW:	70kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	94
PERCENT COVERAGE:	44

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

CCGACACCAT CGAATGGTGC AAAACCTTTC GCGGTATGGC ATGATAGCGC CCGGAAGAGA GTCAATTCAG GGTGGTGAAT GTGAAACCAG TAACGTTATA CGATGTCGCA GAGTATGCCG GTGTCTCTTA TCAGACCGTT TCCCGCGTGG TGAACCAGGC CAGCCACGTT TCTGCGAAAA CGCGGGAAAA AGTGGAAGCG GCGATGGCGG AGCTGAATTA CATTCCCAAC CGCGTGGCAC AACAACTGGC GGGCAAACAG TCGTTGCTGA TTGGCGTTGC CACCTCCAGT CTGGCCCTGC ACGCGCCGTC GCAAATTGTC GCGGCGATTA AATCTCGCGC CGATCAACTG GGTGCCAGCG TGGTGGTGTC GATGGTAGAA CGAAGCGGCG TCGAAGCCTG TAAAGCGGCG GTGCACAATC TTCTCGCGCA ACGCGTCAGT GGGCTGATCA TTAACTATCC GCTGGATGAC CAGGATGCCA TTGCTGTGGA AGCTGCCTGC ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA CACCCATCAA CAGTATTATT TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC TGGTCGCATT GGGTCACCAG CAAATCGCGC TGTTAGCGGG CCCATTAAGT TCTGTCTCGG CGCGTCTGCG TCTGGCTGGC TGGCATAAAT ATCTCACTCG CAATCAAATT CAGCCGATAG CGGAACGGGA AGGCGACTGG AGTGCCATGT CCGGTTTTCA ACAAACCATG CAAATGCTGA ATGAGGGCAT CGTTCCCACT GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA TGCGCGCCAT TACCGAGTCC GGGCTGCGCG TTGGTGCGGA TATCTCGGTA GTGGGATACG ACGATACCGA AGACAGCTCA TGTTATATCC CGCCGTTAAC CACCATCAAA CAGGATTTTC GCCTGCTGGG GCAAACCAGC GTGGACCGCT TGCTGCAACT CTCTCAGGGC CAGGCGGTGA AGGGCAATCA GCTGTTGCCC GTCTCACTGG TGAAAAGAAA AACCACCCTG GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TAAGTTAGCT CACTCATTAG GCACAATTCT CATGTTTGAC AGCTTATCAT CGACTGCACG GTGCACCAAT GCTTCTGGCG TCAGGCAGCC ATCGGAAGCT GTGGTATGGC TGTGCAGGTC GTAAATCACT GCATAATTCG TGTCGCTCAA GGCGCACTCC CGTTCTGGAT AATGTTTTTT GCGCCGACAT CATAACGGTT CTGGCAAATA TTCTGAAATG AGCTGTTGAC AATTAATCAT CGGCTCGTAT AATGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCCAGT CCGTTTAGGT GTTTTCACGA GCACTTCACC AACAAGGACC ATAGCATATG AAAATCGAAG AAGGTAAACT GGTAATCTGG ATTAACGGCG ATAAAGGCTA TAACGGTCTC GCTGAAGTCG GTAAGAAATT CGAGAAAGAT ACCGGAATTA AAGTCACCGT TGAGCATCCG GATAAACTGG AAGAGAAATT CCCACAGGTT GCGGCAACTG GCGATGGCCC TGACATTATC TTCTGGGCAC ACGACCGCTT TGGTGGCTAC GCTCAATCTG GCCTGTTGGC TGAAATCACC CCGGACAAAG CGTTCCAGGA CAAGCTGTAT CCGTTTACCT GGGATGCCGT ACGTTACAAC GGCAAGCTGA TTGCTTACCC GATCGCTGTT GAAGCGTTAT CGCTGATTTA TAACAAAGAT CTGCTGCCGA ACCCGCCAAA AACCTGGGAA GAGATCCCGG CGCTGGATAA AGAACTGAAA GCGAAAGGTA AGAGCGCGCT GATGTTCAAC CTGCAAGAAC CGTACTTCAC CTGGCCGCTG ATTGCTGCTG ACGGGGGTTA TGCGTTCAAG TATGAAAACG GCAAGTACGA CATTAAAGAC GTGGGCGTGG ATAACGCTGG CGCGAAAGCG GGTCTGACCT TCCTGGTTGA CCTGATTAAA AACAAACACA TGAATGCAGA CACCGATTAC TCCATCGCAG AAGCTGCCTT TAATAAAGGC GAAACAGCGA TGACCATCAA CGGCCCGTGG GCATGGTCCA ACATCGACAC CAGCAAAGTG AATTATGGTG TAACGGTACT GCCGACCTTC AAGGGTCAAC CATCCAAACC GTTCGTTGGC GTGCTGAGCG CAGGTATTAA CGCCGCCAGT CCGAACAAAG AGCTGGCAAA AGAGTTCCTC GAAAACTATC TGCTGACTGA TGAAGGTCTG GAAGCGGTTA ATAAAGACAA ACCGCTGGGT GCCGTAGCGC TGAAGTCTTA CGAGGAAGAG TTGGCGAAAG ATCCACGTAT TGCCGCCACT ATGGAAAACG CCCAGAAAGG TGAAATCATG CCGAACATCC CGCAGATGTC CGCTTTCTGG TATGCCGTGC GTACTGCGGT GATCAACGCC GCCAGCGGTC GTCAGACTGT CGATGCAGCC CTGGCAGCCG CGCAGACTAA TTCAACAAAT ACAGATTCCG TAGATTCTAC GAATGCTCAA CAAGTAACTA AAAATACTGA AGCTAAAGGT GTTGCTACAA ACAAATCAGC TCAAGATAAT CCAACAATAA AAATGGGAGC TAATGCAAGC TACGTAGTAG GCTATCAAGT AGGTGCTGGT ATAGCTAAGC AAGATTTTGG CTTATACGAT AAGCAAACAA TAGCCGGTTT TGCAGATGCT ATCAATGGTA ATAAGCCAAG AATCTCTGAG AGTCAAATCC GCAGAAATAT GGAAACTCTT AAAGATAAGA TGATTAAAAA ACAACTAGAT ACAGCTAATT TAAATAAAAC AAAATCACAA GAATTTATGG CACAAATTGC TAAAATGGAT AATGCTATAA AAGTTGATGA TGGTGTTTAC TATCAAATGA TTAAGCAAGG CGATGGCAAA AATCCTAAAT CTGATAGTCA AGTTACAATA GCTTATAAAG GAACTACGCC AGTTATAGCT TACGAAGATG ATAAATCGAA ACTAAACGAG GTCAAAGAAG CTAAACTAAT TGGTCCTACA TTTGACTCGA GTGATAGCGC AACATTCCCA CTCAGAAACC TAATCGAATG TTGGAAAGAT GCTATTCCTC AAATACCTAA TGGATCAACA ATAATATTAT ATTGCTCTCC AGATAAGGCA TATGGAACTA GAGCGCCCGC TGTCATAGGA CCTAATCAAG CACTTTCTTT TGAAATTACG CTTAAAGATT TTAAAGCGGC CGCAGCTAGC GAATTCGGAT CCTCTAGAGT CGACCTGCAG GCAAGCTTGG CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGCA GCTTGGCTGT TTTGGCGGAT GAGATAAGAT TTTCAGCCTG ATACAGATTA AATCAGAACG CAGAAGCGGT CTGATAAAAC AGAATTTGCC TGGCGGCAGT AGCGCGGTGG TCCCACCTGA CCCCATGCCG AACTCAGAAG TGAAACGCCG TAGCGCCGAT GGTAGTGTGG GGTCTCCCCA TGCGAGAGTA GGGAACTGCC AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCGCCGG GAGCGGATTT GAACGTTGCG AAGCAACGGC CCGGAGGGTG GCGGGCAGGA CGCCCGCCAT AAACTGCCAG GCATCAAATT AAGCAGAAGG CCATCCTGAC GGATGGCCTT TTTGCGTTTC TACAAACTCT TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTCCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTACCCCG GTTGATAATC AGAAAAGCCC CAAAAACAGG AAGATTGTAT AAGCAAATAT TTAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CAAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGCAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAAAT T

Details for FrtuB.00130.a.HC21.PW37897

PURIFICATION DATe:	4/18/2016
CONCENTRATION:	38mg/ml
OBSERVED MW:	70kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	94
PERCENT COVERAGE:	44

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)