FrtuB.00020.a

Aldehyde dehydrogenase

CENTER ID: FrtuB.00020.a
ORGANISM: Francisella tularensis subsp. tularensis SCHU S4 / Schu 4
ASSOCIATED DISEASE: tularemia
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIA

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
FrtuB.00020.a.B1.GE39265 Full length(FrtuB.00020.a) 1 498
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
FrtuB.00020.a.B1.PS37973 Full length(FrtuB.00020.a) 1 498
FrtuB.00020.a.B1.PS37991 Full length(FrtuB.00020.a) 1 498

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|177416.36.peg.285
RefSeq: YP_169578.1
UniProt: Q5NHB4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSILKELDLG LQAYITNDTN NVIETLNPAT GELLAKVRNQ SVTTMQEAIA KATEVAKQWR QVPAPKRGEL VRLIDEELRR NKDHLGSLVS LEMGKSKQEG DGEVQEMIDM ADFAVGQSRM LYGMMMNSER HNHRMYEQWH PLGVVGVISA FNFPVAVWSW NAFIAVICGN TVVWKPSEKI PLCSIAVHNI CQKVIKEHNY PEIFYTVISK DVEVSKTLVN DERVNLVSFT GSTKVGQDVG QQVAKRFGKS ILELGGNNAT IIDESANLKL AIPAAVFGAV GTAGQRCTSL RRLFIHESIY DLVKEKMVNA YKQVKVGDPL DQANLMGPLI DQAAVDNFTR TVEQAINQGG KVLTGGKSIA KPGFFVEPTI IEANHNMPIV AEENFCPILY IMPFKDIDEA IALNNSVIYG LSSSIFTDNL QNAEKFLSSL GSDCGIANVN IGTSGAEIGG AFGGEKHTGG GREAGSDAWK AYMRRQTSTI NYGKDLPLAQ GIKFNLGE
NT Sequence
ATGAGTATTT TAAAAGAGCT AGATTTAGGT TTACAAGCAT ATATCACTAA CGATACTAAT AACGTTATAG AAACTTTAAA TCCTGCAACT GGTGAGCTAT TAGCTAAAGT TAGAAACCAA AGTGTAACTA CTATGCAGGA GGCTATAGCA AAAGCTACAG AAGTTGCTAA GCAATGGCGA CAAGTTCCTG CACCTAAAAG AGGCGAGTTG GTTAGACTTA TCGATGAGGA ATTACGTAGA AACAAAGATC ATCTAGGTAG TCTAGTATCT CTTGAGATGG GTAAATCTAA ACAAGAGGGT GATGGTGAAG TCCAGGAAAT GATAGATATG GCAGATTTTG CTGTAGGACA ATCGCGTATG CTTTATGGAA TGATGATGAA TTCTGAGCGC CATAATCATA GAATGTATGA GCAATGGCAT CCACTAGGTG TTGTTGGAGT TATCTCGGCT TTTAACTTTC CTGTAGCAGT GTGGTCATGG AATGCTTTTA TAGCTGTAAT TTGTGGTAAT ACAGTAGTCT GGAAACCATC AGAAAAAATA CCTTTGTGTT CTATCGCGGT ACATAATATT TGTCAAAAAG TAATTAAAGA GCATAATTAC CCTGAAATTT TTTACACAGT CATTTCTAAA GATGTTGAGG TTTCAAAAAC ATTAGTTAAT GATGAAAGAG TAAATCTAGT ATCTTTCACA GGTTCAACTA AGGTAGGGCA AGATGTTGGT CAGCAAGTTG CTAAAAGATT TGGTAAGTCG ATTTTAGAAT TAGGTGGTAA TAATGCTACA ATTATTGATG AATCCGCTAA TTTGAAACTT GCAATTCCTG CAGCAGTTTT TGGGGCAGTT GGTACAGCTG GTCAAAGATG TACTTCGCTA AGAAGACTCT TTATCCATGA GTCGATCTAT GATTTAGTCA AAGAGAAAAT GGTCAATGCT TACAAGCAAG TCAAAGTTGG AGATCCTCTT GATCAAGCTA ATTTAATGGG ACCTCTTATA GATCAAGCTG CGGTTGATAA TTTCACTAGA ACTGTAGAGC AAGCTATTAA TCAAGGTGGT AAAGTTCTAA CAGGTGGTAA AAGTATTGCA AAGCCTGGCT TTTTTGTTGA GCCGACAATT ATCGAAGCCA ATCATAATAT GCCAATCGTT GCTGAAGAGA ACTTTTGTCC AATATTATAC ATAATGCCTT TTAAAGATAT TGATGAAGCT ATAGCACTCA ATAACAGTGT AATCTACGGT TTATCAAGCT CTATATTTAC GGACAATCTT CAAAATGCTG AAAAGTTCTT ATCTAGCTTA GGTAGTGATT GTGGTATAGC TAATGTTAAT ATTGGTACCT CTGGAGCAGA GATTGGAGGA GCTTTTGGTG GTGAGAAGCA TACTGGAGGA GGACGTGAAG CTGGTTCAGA TGCTTGGAAA GCATATATGC GTCGTCAGAC TAGTACTATT AACTATGGTA AAGATTTACC ATTAGCACAA GGAATTAAAT TTAATCTAGG GGAG
Details for FrtuB.00020.a.B1.GE39265
HARVESTED ON: 6/10/2015
SEQUENCED ON: 6/5/2015
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS ILKELDLGLQ AYITNDTNNV IETLNPATGE LLAKVRNQSV TTMQEAIAKA TEVAKQWRQV PAPKRGELVR LIDEELRRNK DHLGSLVSLE MGKSKQEGDG EVQEMIDMAD FAVGQSRMLY GMMMNSERHN HRMYEQWHPL GVVGVISAFN FPVAVWSWNA FIAVICGNTV VWKPSEKIPL CSIAVHNICQ KVIKEHNYPE IFYTVISKDV EVSKTLVNDE RVNLVSFTGS TKVGQDVGQQ VAKRFGKSIL ELGGNNATII DESANLKLAI PAAVFGAVGT AGQRCTSLRR LFIHESIYDL VKEKMVNAYK QVKVGDPLDQ ANLMGPLIDQ AAVDNFTRTV EQAINQGGKV LTGGKSIAKP GFFVEPTIIE ANHNMPIVAE ENFCPILYIM PFKDIDEAIA LNNSVIYGLS SSIFTDNLQN AEKFLSSLGS DCGIANVNIG TSGAEIGGAF GGEKHTGGGR EAGSDAWKAY MRRQTSTINY GKDLPLA
Validated NT Sequence
tgtgctaatg gtaaatcttt accatagtta atagtactag tctgacgacg catatatgct ttccaagcat ctgaaccagc ttcacgtcct cctccagtat gcttctcacc accaaaagct cctccaatct ctgctccaga ggtaccaata ttaacattag ctataccaca atcactacct aagctagata agaacttttc agcattttga agattgtccg taaatataga gcttgataaa ccgtagatta cactgttatt gagtgctata gcttcatcaa tatctttaaa aggcattatg tataatattg gacaaaagtt ctcttcagca acgattggca tattatgatt ggcttcgata attgtcggct caacaaaaaa gccaggcttt gcaatacttt taccacctgt tagaacttta ccaccttgat taatagcttg ctctacagtt ctagtgaaat tatcaaccgc agcttgatct ataagaggtc ccattaaatt agcttgatca agaggatctc caactttgac ttgcttgtaa gcattgacca ttttctcttt gactaaatca tagatcgact catggataaa gagtcttctt agcgaagtac atctttgacc agctgtacca actgccccaa aaactgctgc aggaattgca agtttcaaat tagcggattc atcaataatt gtagcattat taccacctaa ttctaaaatc gacttaccaa atcttttagc aacttgctga ccaacatctt gccctacctt agttgaacct gtgaaagata ctagatttac tctttcatca ttaactaatg tttttgaaac ctcaacatct ttagaaatga ctgtgtaaaa aatttcaggg taattatgct ctttaattac tttttgacaa atattatgta ccgcgataga acacaaaggt attttttctg atggtttcca gactactgta ttaccacaaa ttacagctat aaaagcattc catgaccaca ctgctacagg aaagttaaaa gccgagataa ctccaacaac acctagtgga tgccattgct catacattct atgattatgg cgctcagaat tcatcatcat tccataaagc atacgcgatt gtcctacagc aaaatctgcc atatctatca tttcctggac ttcaccatca ccctcttgtt tagatttacc catctcaaga gatactagac tacctagatg atctttgttt ctacgtaatt cctcatcgat aagtctaacc aactcgcctc ttttaggtgc aggaacttgt cgccattgct tagcaacttc tgtagctttt gctatagcct cctgcatagt agttacactt tggtttctaa ctttagctaa tagctcacca gttgcaggat ttaaagtttc tataacgtta ttagtatcgt tagtgatata tgcttgtaaa cctaaatcta gctcttttaa aatactcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMS ILKELDLGLQ AYITNDTNNV IETLNPATGE LLAKVRNQSV TTMQEAIAKA TEVAKQWRQV PAPKRGELVR LIDEELRRNK DHLGSLVSLE MGKSKQEGDG EVQEMIDMAD FAVGQSRMLY GMMMNSERHN HRMYEQWHPL GVVGVISAFN FPVAVWSWNA FIAVICGNTV VWKPSEKIPL CSIAVHNICQ KVIKEHNYPE IFYTVISKDV EVSKTLVNDE RVNLVSFTGS TKVGQDVGQQ VAKRFGKSIL ELGGNNATII DESANLKLAI PAAVFGAVGT AGQRCTSLRR LFIHESIYDL VKEKMVNAYK QVKVGDPLDQ ANLMGPLIDQ AAVDNFTRTV EQAINQGGKV LTGGKSIAKP GFFVEPTIIE ANHNMPIVAE ENFCPILYIM PFKDIDEAIA LNNSVIYGLS SSIFTDNLQN AEKFLSSLGS DCGIANVNIG TSGAEIGGAF GGEKHTGGGR EAGSDAWKAY MRRQTSTINY GKDLPLAQGI KFNLGE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta ttttaaaaga gctagattta ggtttacaag catatatcac taacgatact aataacgtta tagaaacttt aaatcctgca actggtgagc tattagctaa agttagaaac caaagtgtaa ctactatgca ggaggctata gcaaaagcta cagaagttgc taagcaatgg cgacaagttc ctgcacctaa aagaggcgag ttggttagac ttatcgatga ggaattacgt agaaacaaag atcatctagg tagtctagta tctcttgaga tgggtaaatc taaacaagag ggtgatggtg aagtccagga aatgatagat atggcagatt ttgctgtagg acaatcgcgt atgctttatg gaatgatgat gaattctgag cgccataatc atagaatgta tgagcaatgg catccactag gtgttgttgg agttatctcg gcttttaact ttcctgtagc agtgtggtca tggaatgctt ttatagctgt aatttgtggt aatacagtag tctggaaacc atcagaaaaa atacctttgt gttctatcgc ggtacataat atttgtcaaa aagtaattaa agagcataat taccctgaaa ttttttacac agtcatttct aaagatgttg aggtttcaaa aacattagtt aatgatgaaa gagtaaatct agtatctttc acaggttcaa ctaaggtagg gcaagatgtt ggtcagcaag ttgctaaaag atttggtaag tcgattttag aattaggtgg taataatgct acaattattg atgaatccgc taatttgaaa cttgcaattc ctgcagcagt ttttggggca gttggtacag ctggtcaaag atgtacttcg ctaagaagac tctttatcca tgagtcgatc tatgatttag tcaaagagaa aatggtcaat gcttacaagc aagtcaaagt tggagatcct cttgatcaag ctaatttaat gggacctctt atagatcaag ctgcggttga taatttcact agaactgtag agcaagctat taatcaaggt ggtaaagttc taacaggtgg taaaagtatt gcaaagcctg gcttttttgt tgagccgaca attatcgaag ccaatcataa tatgccaatc gttgctgaag agaacttttg tccaatatta tacataatgc cttttaaaga tattgatgaa gctatagcac tcaataacag tgtaatctac ggtttatcaa gctctatatt tacggacaat cttcaaaatg ctgaaaagtt cttatctagc ttaggtagtg attgtggtat agctaatgtt aatattggta cctctggagc agagattgga ggagcttttg gtggtgagaa gcatactgga ggaggacgtg aagctggttc agatgcttgg aaagcatata tgcgtcgtca gactagtact attaactatg gtaaagattt accattagca caaggaatta aatttaatct aggggagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for FrtuB.00020.a.B1.PS37973
PURIFICATION DATe: 7/13/2016
CONCENTRATION: 28.2mg/ml
OBSERVED MW: 52kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS ILKELDLGLQ AYITNDTNNV IETLNPATGE LLAKVRNQSV TTMQEAIAKA TEVAKQWRQV PAPKRGELVR LIDEELRRNK DHLGSLVSLE MGKSKQEGDG EVQEMIDMAD FAVGQSRMLY GMMMNSERHN HRMYEQWHPL GVVGVISAFN FPVAVWSWNA FIAVICGNTV VWKPSEKIPL CSIAVHNICQ KVIKEHNYPE IFYTVISKDV EVSKTLVNDE RVNLVSFTGS TKVGQDVGQQ VAKRFGKSIL ELGGNNATII DESANLKLAI PAAVFGAVGT AGQRCTSLRR LFIHESIYDL VKEKMVNAYK QVKVGDPLDQ ANLMGPLIDQ AAVDNFTRTV EQAINQGGKV LTGGKSIAKP GFFVEPTIIE ANHNMPIVAE ENFCPILYIM PFKDIDEAIA LNNSVIYGLS SSIFTDNLQN AEKFLSSLGS DCGIANVNIG TSGAEIGGAF GGEKHTGGGR EAGSDAWKAY MRRQTSTINY GKDLPLA
Validated NT Sequence
tgtgctaatg gtaaatcttt accatagtta atagtactag tctgacgacg catatatgct ttccaagcat ctgaaccagc ttcacgtcct cctccagtat gcttctcacc accaaaagct cctccaatct ctgctccaga ggtaccaata ttaacattag ctataccaca atcactacct aagctagata agaacttttc agcattttga agattgtccg taaatataga gcttgataaa ccgtagatta cactgttatt gagtgctata gcttcatcaa tatctttaaa aggcattatg tataatattg gacaaaagtt ctcttcagca acgattggca tattatgatt ggcttcgata attgtcggct caacaaaaaa gccaggcttt gcaatacttt taccacctgt tagaacttta ccaccttgat taatagcttg ctctacagtt ctagtgaaat tatcaaccgc agcttgatct ataagaggtc ccattaaatt agcttgatca agaggatctc caactttgac ttgcttgtaa gcattgacca ttttctcttt gactaaatca tagatcgact catggataaa gagtcttctt agcgaagtac atctttgacc agctgtacca actgccccaa aaactgctgc aggaattgca agtttcaaat tagcggattc atcaataatt gtagcattat taccacctaa ttctaaaatc gacttaccaa atcttttagc aacttgctga ccaacatctt gccctacctt agttgaacct gtgaaagata ctagatttac tctttcatca ttaactaatg tttttgaaac ctcaacatct ttagaaatga ctgtgtaaaa aatttcaggg taattatgct ctttaattac tttttgacaa atattatgta ccgcgataga acacaaaggt attttttctg atggtttcca gactactgta ttaccacaaa ttacagctat aaaagcattc catgaccaca ctgctacagg aaagttaaaa gccgagataa ctccaacaac acctagtgga tgccattgct catacattct atgattatgg cgctcagaat tcatcatcat tccataaagc atacgcgatt gtcctacagc aaaatctgcc atatctatca tttcctggac ttcaccatca ccctcttgtt tagatttacc catctcaaga gatactagac tacctagatg atctttgttt ctacgtaatt cctcatcgat aagtctaacc aactcgcctc ttttaggtgc aggaacttgt cgccattgct tagcaacttc tgtagctttt gctatagcct cctgcatagt agttacactt tggtttctaa ctttagctaa tagctcacca gttgcaggat ttaaagtttc tataacgtta ttagtatcgt tagtgatata tgcttgtaaa cctaaatcta gctcttttaa aatactcata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMS ILKELDLGLQ AYITNDTNNV IETLNPATGE LLAKVRNQSV TTMQEAIAKA TEVAKQWRQV PAPKRGELVR LIDEELRRNK DHLGSLVSLE MGKSKQEGDG EVQEMIDMAD FAVGQSRMLY GMMMNSERHN HRMYEQWHPL GVVGVISAFN FPVAVWSWNA FIAVICGNTV VWKPSEKIPL CSIAVHNICQ KVIKEHNYPE IFYTVISKDV EVSKTLVNDE RVNLVSFTGS TKVGQDVGQQ VAKRFGKSIL ELGGNNATII DESANLKLAI PAAVFGAVGT AGQRCTSLRR LFIHESIYDL VKEKMVNAYK QVKVGDPLDQ ANLMGPLIDQ AAVDNFTRTV EQAINQGGKV LTGGKSIAKP GFFVEPTIIE ANHNMPIVAE ENFCPILYIM PFKDIDEAIA LNNSVIYGLS SSIFTDNLQN AEKFLSSLGS DCGIANVNIG TSGAEIGGAF GGEKHTGGGR EAGSDAWKAY MRRQTSTINY GKDLPLAQGI KFNLGE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTA TTTTAAAAGA GCTAGATTTA GGTTTACAAG CATATATCAC TAACGATACT AATAACGTTA TAGAAACTTT AAATCCTGCA ACTGGTGAGC TATTAGCTAA AGTTAGAAAC CAAAGTGTAA CTACTATGCA GGAGGCTATA GCAAAAGCTA CAGAAGTTGC TAAGCAATGG CGACAAGTTC CTGCACCTAA AAGAGGCGAG TTGGTTAGAC TTATCGATGA GGAATTACGT AGAAACAAAG ATCATCTAGG TAGTCTAGTA TCTCTTGAGA TGGGTAAATC TAAACAAGAG GGTGATGGTG AAGTCCAGGA AATGATAGAT ATGGCAGATT TTGCTGTAGG ACAATCGCGT ATGCTTTATG GAATGATGAT GAATTCTGAG CGCCATAATC ATAGAATGTA TGAGCAATGG CATCCACTAG GTGTTGTTGG AGTTATCTCG GCTTTTAACT TTCCTGTAGC AGTGTGGTCA TGGAATGCTT TTATAGCTGT AATTTGTGGT AATACAGTAG TCTGGAAACC ATCAGAAAAA ATACCTTTGT GTTCTATCGC GGTACATAAT ATTTGTCAAA AAGTAATTAA AGAGCATAAT TACCCTGAAA TTTTTTACAC AGTCATTTCT AAAGATGTTG AGGTTTCAAA AACATTAGTT AATGATGAAA GAGTAAATCT AGTATCTTTC ACAGGTTCAA CTAAGGTAGG GCAAGATGTT GGTCAGCAAG TTGCTAAAAG ATTTGGTAAG TCGATTTTAG AATTAGGTGG TAATAATGCT ACAATTATTG ATGAATCCGC TAATTTGAAA CTTGCAATTC CTGCAGCAGT TTTTGGGGCA GTTGGTACAG CTGGTCAAAG ATGTACTTCG CTAAGAAGAC TCTTTATCCA TGAGTCGATC TATGATTTAG TCAAAGAGAA AATGGTCAAT GCTTACAAGC AAGTCAAAGT TGGAGATCCT CTTGATCAAG CTAATTTAAT GGGACCTCTT ATAGATCAAG CTGCGGTTGA TAATTTCACT AGAACTGTAG AGCAAGCTAT TAATCAAGGT GGTAAAGTTC TAACAGGTGG TAAAAGTATT GCAAAGCCTG GCTTTTTTGT TGAGCCGACA ATTATCGAAG CCAATCATAA TATGCCAATC GTTGCTGAAG AGAACTTTTG TCCAATATTA TACATAATGC CTTTTAAAGA TATTGATGAA GCTATAGCAC TCAATAACAG TGTAATCTAC GGTTTATCAA GCTCTATATT TACGGACAAT CTTCAAAATG CTGAAAAGTT CTTATCTAGC TTAGGTAGTG ATTGTGGTAT AGCTAATGTT AATATTGGTA CCTCTGGAGC AGAGATTGGA GGAGCTTTTG GTGGTGAGAA GCATACTGGA GGAGGACGTG AAGCTGGTTC AGATGCTTGG AAAGCATATA TGCGTCGTCA GACTAGTACT ATTAACTATG GTAAAGATTT ACCATTAGCA CAAGGAATTA AATTTAATCT AGGGGAGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT
Details for FrtuB.00020.a.B1.PS37991
PURIFICATION DATe: 7/27/2016
CONCENTRATION: 30.9mg/ml
OBSERVED MW: 52kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS ILKELDLGLQ AYITNDTNNV IETLNPATGE LLAKVRNQSV TTMQEAIAKA TEVAKQWRQV PAPKRGELVR LIDEELRRNK DHLGSLVSLE MGKSKQEGDG EVQEMIDMAD FAVGQSRMLY GMMMNSERHN HRMYEQWHPL GVVGVISAFN FPVAVWSWNA FIAVICGNTV VWKPSEKIPL CSIAVHNICQ KVIKEHNYPE IFYTVISKDV EVSKTLVNDE RVNLVSFTGS TKVGQDVGQQ VAKRFGKSIL ELGGNNATII DESANLKLAI PAAVFGAVGT AGQRCTSLRR LFIHESIYDL VKEKMVNAYK QVKVGDPLDQ ANLMGPLIDQ AAVDNFTRTV EQAINQGGKV LTGGKSIAKP GFFVEPTIIE ANHNMPIVAE ENFCPILYIM PFKDIDEAIA LNNSVIYGLS SSIFTDNLQN AEKFLSSLGS DCGIANVNIG TSGAEIGGAF GGEKHTGGGR EAGSDAWKAY MRRQTSTINY GKDLPLA
Validated NT Sequence
tgtgctaatg gtaaatcttt accatagtta atagtactag tctgacgacg catatatgct ttccaagcat ctgaaccagc ttcacgtcct cctccagtat gcttctcacc accaaaagct cctccaatct ctgctccaga ggtaccaata ttaacattag ctataccaca atcactacct aagctagata agaacttttc agcattttga agattgtccg taaatataga gcttgataaa ccgtagatta cactgttatt gagtgctata gcttcatcaa tatctttaaa aggcattatg tataatattg gacaaaagtt ctcttcagca acgattggca tattatgatt ggcttcgata attgtcggct caacaaaaaa gccaggcttt gcaatacttt taccacctgt tagaacttta ccaccttgat taatagcttg ctctacagtt ctagtgaaat tatcaaccgc agcttgatct ataagaggtc ccattaaatt agcttgatca agaggatctc caactttgac ttgcttgtaa gcattgacca ttttctcttt gactaaatca tagatcgact catggataaa gagtcttctt agcgaagtac atctttgacc agctgtacca actgccccaa aaactgctgc aggaattgca agtttcaaat tagcggattc atcaataatt gtagcattat taccacctaa ttctaaaatc gacttaccaa atcttttagc aacttgctga ccaacatctt gccctacctt agttgaacct gtgaaagata ctagatttac tctttcatca ttaactaatg tttttgaaac ctcaacatct ttagaaatga ctgtgtaaaa aatttcaggg taattatgct ctttaattac tttttgacaa atattatgta ccgcgataga acacaaaggt attttttctg atggtttcca gactactgta ttaccacaaa ttacagctat aaaagcattc catgaccaca ctgctacagg aaagttaaaa gccgagataa ctccaacaac acctagtgga tgccattgct catacattct atgattatgg cgctcagaat tcatcatcat tccataaagc atacgcgatt gtcctacagc aaaatctgcc atatctatca tttcctggac ttcaccatca ccctcttgtt tagatttacc catctcaaga gatactagac tacctagatg atctttgttt ctacgtaatt cctcatcgat aagtctaacc aactcgcctc ttttaggtgc aggaacttgt cgccattgct tagcaacttc tgtagctttt gctatagcct cctgcatagt agttacactt tggtttctaa ctttagctaa tagctcacca gttgcaggat ttaaagtttc tataacgtta ttagtatcgt tagtgatata tgcttgtaaa cctaaatcta gctcttttaa aatactcata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMS ILKELDLGLQ AYITNDTNNV IETLNPATGE LLAKVRNQSV TTMQEAIAKA TEVAKQWRQV PAPKRGELVR LIDEELRRNK DHLGSLVSLE MGKSKQEGDG EVQEMIDMAD FAVGQSRMLY GMMMNSERHN HRMYEQWHPL GVVGVISAFN FPVAVWSWNA FIAVICGNTV VWKPSEKIPL CSIAVHNICQ KVIKEHNYPE IFYTVISKDV EVSKTLVNDE RVNLVSFTGS TKVGQDVGQQ VAKRFGKSIL ELGGNNATII DESANLKLAI PAAVFGAVGT AGQRCTSLRR LFIHESIYDL VKEKMVNAYK QVKVGDPLDQ ANLMGPLIDQ AAVDNFTRTV EQAINQGGKV LTGGKSIAKP GFFVEPTIIE ANHNMPIVAE ENFCPILYIM PFKDIDEAIA LNNSVIYGLS SSIFTDNLQN AEKFLSSLGS DCGIANVNIG TSGAEIGGAF GGEKHTGGGR EAGSDAWKAY MRRQTSTINY GKDLPLAQGI KFNLGE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTA TTTTAAAAGA GCTAGATTTA GGTTTACAAG CATATATCAC TAACGATACT AATAACGTTA TAGAAACTTT AAATCCTGCA ACTGGTGAGC TATTAGCTAA AGTTAGAAAC CAAAGTGTAA CTACTATGCA GGAGGCTATA GCAAAAGCTA CAGAAGTTGC TAAGCAATGG CGACAAGTTC CTGCACCTAA AAGAGGCGAG TTGGTTAGAC TTATCGATGA GGAATTACGT AGAAACAAAG ATCATCTAGG TAGTCTAGTA TCTCTTGAGA TGGGTAAATC TAAACAAGAG GGTGATGGTG AAGTCCAGGA AATGATAGAT ATGGCAGATT TTGCTGTAGG ACAATCGCGT ATGCTTTATG GAATGATGAT GAATTCTGAG CGCCATAATC ATAGAATGTA TGAGCAATGG CATCCACTAG GTGTTGTTGG AGTTATCTCG GCTTTTAACT TTCCTGTAGC AGTGTGGTCA TGGAATGCTT TTATAGCTGT AATTTGTGGT AATACAGTAG TCTGGAAACC ATCAGAAAAA ATACCTTTGT GTTCTATCGC GGTACATAAT ATTTGTCAAA AAGTAATTAA AGAGCATAAT TACCCTGAAA TTTTTTACAC AGTCATTTCT AAAGATGTTG AGGTTTCAAA AACATTAGTT AATGATGAAA GAGTAAATCT AGTATCTTTC ACAGGTTCAA CTAAGGTAGG GCAAGATGTT GGTCAGCAAG TTGCTAAAAG ATTTGGTAAG TCGATTTTAG AATTAGGTGG TAATAATGCT ACAATTATTG ATGAATCCGC TAATTTGAAA CTTGCAATTC CTGCAGCAGT TTTTGGGGCA GTTGGTACAG CTGGTCAAAG ATGTACTTCG CTAAGAAGAC TCTTTATCCA TGAGTCGATC TATGATTTAG TCAAAGAGAA AATGGTCAAT GCTTACAAGC AAGTCAAAGT TGGAGATCCT CTTGATCAAG CTAATTTAAT GGGACCTCTT ATAGATCAAG CTGCGGTTGA TAATTTCACT AGAACTGTAG AGCAAGCTAT TAATCAAGGT GGTAAAGTTC TAACAGGTGG TAAAAGTATT GCAAAGCCTG GCTTTTTTGT TGAGCCGACA ATTATCGAAG CCAATCATAA TATGCCAATC GTTGCTGAAG AGAACTTTTG TCCAATATTA TACATAATGC CTTTTAAAGA TATTGATGAA GCTATAGCAC TCAATAACAG TGTAATCTAC GGTTTATCAA GCTCTATATT TACGGACAAT CTTCAAAATG CTGAAAAGTT CTTATCTAGC TTAGGTAGTG ATTGTGGTAT AGCTAATGTT AATATTGGTA CCTCTGGAGC AGAGATTGGA GGAGCTTTTG GTGGTGAGAA GCATACTGGA GGAGGACGTG AAGCTGGTTC AGATGCTTGG AAAGCATATA TGCGTCGTCA GACTAGTACT ATTAACTATG GTAAAGATTT ACCATTAGCA CAAGGAATTA AATTTAATCT AGGGGAGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT