EscoA.18886.a

Lipid A export ATP-binding/permease protein MsbA

CENTER ID: EscoA.18886.a
ORGANISM: Escherichia coli K12
ASSOCIATED DISEASE: Food poisoning
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EscoA.18886.a.B1.GE43042 full length 1 582
EscoA.18886.a.TW12.GE44025 full length 2 582
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EscoA.18886.a.TW12.PD38480 full length 2 582
EscoA.18886.a.TW12.PD38484 full length 2 582

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1411691.4.peg.1362
UniProt: P60752

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHNDKDLSTW QTFRRLWPTI APFKAGLIVA GVALILNAAS DTFMLSLLKP LLDDGFGKTD RSVLVWMPLV VIGLMILRGI TSYVSSYCIS WVSGKVVMTM RRRLFGHMMG MPVSFFDKQS TGTLLSRITY DSEQVASSSS GALITVVREG ASIIGLFIMM FYYSWQLSII LIVLAPIVSI AIRVVSKRFR NISKNMQNTM GQVTTSAEQM LKGHKEVLIF GGQEVETKRF DKVSNRMRLQ GMKMVSASSI SDPIIQLIAS LALAFVLYAA SFPSVMDSLT AGTITVVFSS MIALMRPLKS LTNVNAQFQR GMAACQTLFT ILDSEQEKDE GKRVIERATG DVEFRNVTFT YPGRDVPALR NINLKIPAGK TVALVGRSGS GKSTIASLIT RFYDIDEGEI LMDGHDLREY TLASLRNQVA LVSQNVHLFN DTVANNIAYA RTEQYSREQI EEAARMAYAM DFINKMDNGL DTVIGENGVL LSGGQRQRIA IARALLRDSP ILILDEATSA LDTESERAIQ AALDELQKNR TSLVIAHRLS TIEKADEIVV VEDGVIVERG THNDLLEHRG VYAQLHKMQF GQ
NT Sequence
atgcataacg acaaagatct ctctacgtgg cagacattcc gccgactgtg gccaaccatt gcgcctttca aagcgggtct gatcgtggcg ggcgtagcgt taatcctcaa cgcagccagc gataccttca tgttatcgct ccttaagcca cttcttgatg atggctttgg taaaacagat cgctccgtgc tggtgtggat gccgctggtg gtgatcgggc tgatgatttt acgtggtatc accagctatg tctccagcta ctgtatctcc tgggtatcag gaaaggtggt aatgaccatg cgtcgccgcc tgtttggtca catgatggga atgccagttt cattctttga caaacagtca acgggtacgc tgttgtcacg tattacctac gattccgaac aggttgcttc ttcttcttcc ggcgcactga ttactgttgt gcgtgaaggt gcgtcgatca tcggcctgtt catcatgatg ttctattaca gttggcaact gtcgatcatt ttgattgtgc tggcaccgat tgtttcgatt gcgattcgcg ttgtatcgaa gcgttttcgc aacatcagta aaaacatgca gaacaccatg gggcaggtga ccaccagcgc agaacaaatg ctgaagggcc acaaagaagt attgattttc ggtggtcagg aagtggaaac gaaacgcttt gataaagtca gcaaccgaat gcgtcttcag gggatgaaaa tggtttcagc ctcttccatc tctgatccga tcattcagct gatcgcctct ttggcgctgg cgtttgttct gtatgcggcg agcttcccaa gtgtcatgga tagcctgact gccggtacga ttaccgttgt tttctcttca atgattgcac tgatgcgtcc gctgaaatcg ctgaccaacg ttaacgccca gttccagcgc ggtatggcgg cttgtcagac gctgtttacc attctggaca gtgagcagga gaaagatgaa ggtaagcgcg tgatcgagcg tgcgactggc gacgtggaat tccgcaatgt cacctttact tatccgggac gtgacgtacc tgcattgcgt aacatcaacc tgaaaattcc ggcagggaag acggttgctc tggttggacg ctctggttcg ggtaaatcaa ccatcgccag cctgatcacg cgtttttacg atattgatga aggcgaaatc ctgatggatg gtcacgatct gcgcgagtat accctggcgt cgttacgtaa ccaggttgct ctggtgtcgc agaatgtcca tctgtttaac gatacggttg ctaacaacat tgcttacgca cggactgaac agtacagccg tgagcaaatt gaagaagcgg cgcgtatggc ctacgccatg gacttcatca ataagatgga taacggtctc gatacagtga ttggtgaaaa cggcgtgctg ctctctggcg gtcagcgtca gcgtattgct atcgctcgag ccttgttgcg tgatagcccg attctgattc tggacgaagc tacctcggct ctggataccg aatccgaacg tgcgattcag gcggcactgg atgagttgca gaaaaaccgt acctctctgg tgattgccca ccgcttgtct accattgaaa aggcagacga aatcgtggtc gtcgaggatg gtgtcattgt ggaacgcggt acgcataacg atttgcttga gcaccgcggc gtttacgcgc aacttcacaa aatgcagttt ggccaatga
Details for EscoA.18886.a.B1.GE43042
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 65kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMH NDKDLSTWQT FRRLWPTIAP FKAGLIVAGV ALILNAASDT FMLSLLKPLL DDGFGKTDRS VLVWMPLVVI GLMILRGITS YVSSYCISWV SGKVVMTMRR RLFGHMMGMP VSFFDKQSTG TLLSRITYDS EQVASSSSGA LITVVREGAS IIGLFIMMFY YSWQLSIILI VLAPIVSIAI RVVSKRFRNI SKNMQNTMGQ VTTSAEQMLK GHKEVLIFGG QEVETKRFDK VSNRMRLQGM KMVSASSISD PIIQLIASLA LAFVLYAASF PSVMDSLTAG TITVVFSSMI ALMRPLKSLT NVNAQFQRGM AACQTLFTIL DSEQEKDEGK RVIERATGDV EFRNVTFTYP GRDVPALRNI NLKIPAGKTV ALVGRSGSGK STIASLITRF YDIDEGEILM DGHDLREYTL ASLRNQVALV SQNVHLFNDT VANNIAYART EQYSREQIEE AARMAYAMDF INKMDNGLDT VIGENGVLLS GGQRQRIAIA RALLRDSPIL ILDEATSALD TESERAIQAA LDELQKNRTS LVIAHRLSTI EKADEIVVVE DGVIVERGTH NDLLEHRGVY AQLHKMQFGQ
Validated NT Sequence
atggctcacc accaccacca ccatatgcat aacgacaaag atctctctac gtggcagaca ttccgccgac tgtggccaac cattgcgcct ttcaaagcgg gtctgatcgt ggcgggcgta gcgttaatcc tcaacgcagc cagcgatacc ttcatgttat cgctccttaa gccacttctt gatgatggct ttggtaaaac agatcgctcc gtgctggtgt ggatgccgct ggtggtgatc gggctgatga ttttacgtgg natcaccagc tatgtctcca gctactgtat ctcctgggta tcaggaaagg tggtaatgac catgcgtcgc cgcctgtttg gtcacatgat gggaatgcca gtttcattct ttgacaaaca gtcaacgggt acgctgttgt cacgtattac ctacgattcc gaacaggttg cttcttcttc ttccggcgca ctgattactg ttgtgcgtga aggtgcgtcg atcatcggcc tgttcatcat gatgttctat tacagttggc aactgtcgat cattttgatt gtgctggcac cgattgtttc gattgcgatt cgcgttgtat cgaagcgttt tcgcaacatc agtaaaaa-c atgcagaaca ccatggggca ggtgaccacc agcgcagaac aaatgctgaa gggccacaaa gaagtattga ttttcggtgg tcaggaagtg gaaacgaaac gctttgataa agtcagcaac cgaatgcgtc ttcaggggat gaaaatggtt tcagcctctt ccatctctga tccgatcatt cagctgatcg cctctttggc gctggcg-tt tgttctgtat gcggcgagct tcccaagtgt catggatagc ctgactgccg gtacgattac cgttgttttc tcttcaatga ttgcactgat gcgtccgctg aaatcgctga ccaacgttaa cgcccagttc cagcgcggta tggcggcttg tcagacgctg tttaccattc tggacagtga gcaggagaaa gatgaaggta agcgcgtgat cgagcgtgcg actggcgacg tggaattccg caatgtcacc tttacttatc cgggacgtga cgtacctgca ttgcgtaaca tcaacctgaa aattccggca gggaagacgg ttgctctggt tggacgctct ggttcgggta aatcaaccat cgccagcctg atcacgcgtt tttacgatat tgatgaaggc gaaatcctga tggatggtca cgatctgcgc gagtataccc tggcgtcgtt acgtaaccag gttgctctgg tgtcgcagaa tgtccatctg tttaacgata cggttgctaa caacattgct tacgcacgga ctgaacagta cagccgtgag caaattgaag aagcggcgcg tatggcctac gccatggact tcatcaataa gatggataac ggtctcgata cagtgattgg tgaaaacggc gtgctgctct ctggcggtca gcgtcagcgt attgctatcg ctcgagcctt gttgcgtgat agcccgattc tgattctgga cgaagctacc tcggctctgg ataccgaatc cgaacgtgcg attcaggcgg cactggatga gttgcagaaa aaccgtacct ctctggtgat tgcccaccgc ttgtctacca ttgaaaaggc agacgaaatc gtggtcgtcg aggatggtgt cattgtggaa cgcggtacgc ataacgattt gcttgagcac cgcggcgttt acgcgcaact tcacaaaatg cagtttggcc aataa
Expected Protein Sequence
MAHHHHHHMH NDKDLSTWQT FRRLWPTIAP FKAGLIVAGV ALILNAASDT FMLSLLKPLL DDGFGKTDRS VLVWMPLVVI GLMILRGITS YVSSYCISWV SGKVVMTMRR RLFGHMMGMP VSFFDKQSTG TLLSRITYDS EQVASSSSGA LITVVREGAS IIGLFIMMFY YSWQLSIILI VLAPIVSIAI RVVSKRFRNI SKNMQNTMGQ VTTSAEQMLK GHKEVLIFGG QEVETKRFDK VSNRMRLQGM KMVSASSISD PIIQLIASLA LAFVLYAASF PSVMDSLTAG TITVVFSSMI ALMRPLKSLT NVNAQFQRGM AACQTLFTIL DSEQEKDEGK RVIERATGDV EFRNVTFTYP GRDVPALRNI NLKIPAGKTV ALVGRSGSGK STIASLITRF YDIDEGEILM DGHDLREYTL ASLRNQVALV SQNVHLFNDT VANNIAYART EQYSREQIEE AARMAYAMDF INKMDNGLDT VIGENGVLLS GGQRQRIAIA RALLRDSPIL ILDEATSALD TESERAIQAA LDELQKNRTS LVIAHRLSTI EKADEIVVVE DGVIVERGTH NDLLEHRGVY AQLHKMQFGQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcata acgacaaaga tctctctacg tggcagacat tccgccgact gtggccaacc attgcgcctt tcaaagcggg tctgatcgtg gcgggcgtag cgttaatcct caacgcagcc agcgatacct tcatgttatc gctccttaag ccacttcttg atgatggctt tggtaaaaca gatcgctccg tgctggtgtg gatgccgctg gtggtgatcg ggctgatgat tttacgtggt atcaccagct atgtctccag ctactgtatc tcctgggtat caggaaaggt ggtaatgacc atgcgtcgcc gcctgtttgg tcacatgatg ggaatgccag tttcattctt tgacaaacag tcaacgggta cgctgttgtc acgtattacc tacgattccg aacaggttgc ttcttcttct tccggcgcac tgattactgt tgtgcgtgaa ggtgcgtcga tcatcggcct gttcatcatg atgttctatt acagttggca actgtcgatc attttgattg tgctggcacc gattgtttcg attgcgattc gcgttgtatc gaagcgtttt cgcaacatca gtaaaaacat gcagaacacc atggggcagg tgaccaccag cgcagaacaa atgctgaagg gccacaaaga agtattgatt ttcggtggtc aggaagtgga aacgaaacgc tttgataaag tcagcaaccg aatgcgtctt caggggatga aaatggtttc agcctcttcc atctctgatc cgatcattca gctgatcgcc tctttggcgc tggcgtttgt tctgtatgcg gcgagcttcc caagtgtcat ggatagcctg actgccggta cgattaccgt tgttttctct tcaatgattg cactgatgcg tccgctgaaa tcgctgacca acgttaacgc ccagttccag cgcggtatgg cggcttgtca gacgctgttt accattctgg acagtgagca ggagaaagat gaaggtaagc gcgtgatcga gcgtgcgact ggcgacgtgg aattccgcaa tgtcaccttt acttatccgg gacgtgacgt acctgcattg cgtaacatca acctgaaaat tccggcaggg aagacggttg ctctggttgg acgctctggt tcgggtaaat caaccatcgc cagcctgatc acgcgttttt acgatattga tgaaggcgaa atcctgatgg atggtcacga tctgcgcgag tataccctgg cgtcgttacg taaccaggtt gctctggtgt cgcagaatgt ccatctgttt aacgatacgg ttgctaacaa cattgcttac gcacggactg aacagtacag ccgtgagcaa attgaagaag cggcgcgtat ggcctacgcc atggacttca tcaataagat ggataacggt ctcgatacag tgattggtga aaacggcgtg ctgctctctg gcggtcagcg tcagcgtatt gctatcgctc gagccttgtt gcgtgatagc ccgattctga ttctggacga agctacctcg gctctggata ccgaatccga acgtgcgatt caggcggcac tggatgagtt gcagaaaaac cgtacctctc tggtgattgc ccaccgcttg tctaccattg aaaaggcaga cgaaatcgtg gtcgtcgagg atggtgtcat tgtggaacgc ggtacgcata acgatttgct tgagcaccgc ggcgtttacg cgcaacttca caaaatgcag tttggccaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for EscoA.18886.a.TW12.GE44025
HARVESTED ON: 11/18/2020
SEQUENCED ON: 2/1/2023
EXPECTED MW: 69kDa
OBSERVED MW: 69kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL21 DE3
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MGHHHHHHHH HHSGDYKDDD DKGGSSGGDY KDDDDKGGEN LYFQSHNDKD LSTWQTFRRL WPTIAPFKAG LIVAGVALIL NAASDTFMLS LLKPLLDDGF GKTDRSVLVW MPLVVIGLMI LRGITSYVSS YCISWVSGKV VMTMRRRLFG HMMGMPVSFF DKQSTGTLLS RITYDSEQVA SSSSGALITV VREGASIIGL FIMMFYYSWQ LSIILIVLAP IVSIAIRVVS KRFRNISKNM QNTMGQVTTS AEQMLKGHKE VLIFGGQEVE TKRFDKVSNR MRLQGMKMVS ASSISDPIIQ LIASLALAFV LYAASFPSVM DSLTAGTITV VFSSMIALMR PLKSLTNVNA QFQRGMAACQ TLFTILDSEQ EKDEGKRVIE RATGDVEFRN VTFTYPGRDV PALRNINLKI PAGKTVALVG RSGSGKSTIA SLITRFYDID EGEILMDGHD LREYTLASLR NQVALVSQNV HLFNDTVANN IAYARTEQYS REQIEEAARM AYAMDFINKM DNGLDTVIGE NGVLLSGGQR QRIAIARALL RDSPILILDE ATSALDTESE RAIQAALDEL QKNRTSLVIA HRLSTIEKAD EIVVVEDGVI VERGTHNDLL EHRGVYAQLH KMQFGQ
Validated NT Sequence
atgggccacc atcatcacca tcatcaccac caccacagcg gtgactacaa ggacgatgac gataaaggtg gcagcagcgg tggcgattat aaagatgacg acgataaagg tggcgagaac ctgtatttcc agagccataa cgacaaagat ctctctacgt ggcagacatt ccgccgactg tggccaacca ttgcgccttt caaagcgggt ctgatcgtgg cgggcgtagc gttaatcctc aacgcagcca gcgatacctt catgttatcg ctccttaagc cacttcttga tgatggcttt ggtaaaacag atcgctccgt gctggtgtgg atgccgctgg tggtgatcgg gctgatgatt ttacgtggta tcaccagcta tgtctccagc tactgtatct cctgggtatc aggaaaggtg gtaatgacca tgcgtcgccg cctgtttggt cacatgatgg gaatgccagt ttcattcttt gacaaacagt caacgggtac gctgttgtca cgtattacct acgattccga acaggttgct tcttcttctt ccggcgcact gattactgtt gtgcgtgaag gtgcgtcgat catcggcctg ttcatcatga tgttctatta cagttggcaa ctgtcgatca ttttgattgt gctggcaccg attgtttcga ttgcgattcg cgttgtatcg aagcgttttc gcaacatcag taaaaacatg cagaacacca tggggcaggt gaccaccagc gcagaacaaa tgctgaaggg ccacaaagaa gtattgattt tcggtggtca ggaagtggaa acgaaacgct ttgataaagt cagcaaccga atgcgtcttc aggggatgaa aatggtttca gcctcttcca tctctgatcc gatcattcag ctgatcgcct ctttggcgct ggcgtttgtt ctgtatgcgg cgagcttccc aagtgtcatg gatagcctga ctgccggtac gattaccgtt gttttctctt caatgattgc actgatgcgt ccgctgaaat cgctgaccaa cgttaacgcc cagttccagc gcggtatggc ggcttgtcag acgctgttta ccattctgga cagtgagcag gagaaagatg aaggtaagcg cgtgatcgag cgtgcgactg gcgacgtgga attccgcaat gtcaccttta cttatccggg acgtgacgta cctgcattgc gtaacatcaa cctgaaaatt ccggcaggga agacggttgc tctggttgga cgctctggtt cgggtaaatc aaccatcgcc agcctgatca cgcgttttta cgatattgat gaaggcgaaa tcctgatgga tggtcacgat ctgcgcgagt ataccctggc gtcgttacgt aaccaggttg ctctggtgtc gcagaatgtc catctgttta acgatacggt tgctaacaac attgcttacg cacggactga acagtacagc cgtgagcaaa ttgaagaagc ggcgcgtatg gcctacgcca tggacttcat caataagatg gataacggtc tcgatacagt gattggtgaa aacggcgtgc tgctctctgg cggtcagcgt cagcgtattg ctatcgctcg agccttgttg cgtgatagcc cgattctgat tctggacgaa gctacctcgg ctctggatac cgaatccgaa cgtgcgattc aggcggcact ggatgagttg cagaaaaacc gtacctctct ggtgattgcc caccgcttgt ctaccattga aaaggcagac gaaatcgtgg tcgtcgagga tggtgtcatt gtggaacgcg gtacgcataa cgatttgctt gagcaccgcg gcgtttacgc gcaacttcac aaaatgcagt ttggccaatg ataa
Expected Protein Sequence
MGHHHHHHHH HHSGDYKDDD DKGGSSGGDY KDDDDKGGEN LYFQSHNDKD LSTWQTFRRL WPTIAPFKAG LIVAGVALIL NAASDTFMLS LLKPLLDDGF GKTDRSVLVW MPLVVIGLMI LRGITSYVSS YCISWVSGKV VMTMRRRLFG HMMGMPVSFF DKQSTGTLLS RITYDSEQVA SSSSGALITV VREGASIIGL FIMMFYYSWQ LSIILIVLAP IVSIAIRVVS KRFRNISKNM QNTMGQVTTS AEQMLKGHKE VLIFGGQEVE TKRFDKVSNR MRLQGMKMVS ASSISDPIIQ LIASLALAFV LYAASFPSVM DSLTAGTITV VFSSMIALMR PLKSLTNVNA QFQRGMAACQ TLFTILDSEQ EKDEGKRVIE RATGDVEFRN VTFTYPGRDV PALRNINLKI PAGKTVALVG RSGSGKSTIA SLITRFYDID EGEILMDGHD LREYTLASLR NQVALVSQNV HLFNDTVANN IAYARTEQYS REQIEEAARM AYAMDFINKM DNGLDTVIGE NGVLLSGGQR QRIAIARALL RDSPILILDE ATSALDTESE RAIQAALDEL QKNRTSLVIA HRLSTIEKAD EIVVVEDGVI VERGTHNDLL EHRGVYAQLH KMQFGQ
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc atcatcacca tcatcaccac caccacagcg gtgactacaa ggacgatgac gataaaggtg gcagcagcgg tggcgattat aaagatgacg acgataaagg tggcgagaac ctgtatttcc agagccataa cgacaaagat ctctctacgt ggcagacatt ccgccgactg tggccaacca ttgcgccttt caaagcgggt ctgatcgtgg cgggcgtagc gttaatcctc aacgcagcca gcgatacctt catgttatcg ctccttaagc cacttcttga tgatggcttt ggtaaaacag atcgctccgt gctggtgtgg atgccgctgg tggtgatcgg gctgatgatt ttacgtggta tcaccagcta tgtctccagc tactgtatct cctgggtatc aggaaaggtg gtaatgacca tgcgtcgccg cctgtttggt cacatgatgg gaatgccagt ttcattcttt gacaaacagt caacgggtac gctgttgtca cgtattacct acgattccga acaggttgct tcttcttctt ccggcgcact gattactgtt gtgcgtgaag gtgcgtcgat catcggcctg ttcatcatga tgttctatta cagttggcaa ctgtcgatca ttttgattgt gctggcaccg attgtttcga ttgcgattcg cgttgtatcg aagcgttttc gcaacatcag taaaaacatg cagaacacca tggggcaggt gaccaccagc gcagaacaaa tgctgaaggg ccacaaagaa gtattgattt tcggtggtca ggaagtggaa acgaaacgct ttgataaagt cagcaaccga atgcgtcttc aggggatgaa aatggtttca gcctcttcca tctctgatcc gatcattcag ctgatcgcct ctttggcgct ggcgtttgtt ctgtatgcgg cgagcttccc aagtgtcatg gatagcctga ctgccggtac gattaccgtt gttttctctt caatgattgc actgatgcgt ccgctgaaat cgctgaccaa cgttaacgcc cagttccagc gcggtatggc ggcttgtcag acgctgttta ccattctgga cagtgagcag gagaaagatg aaggtaagcg cgtgatcgag cgtgcgactg gcgacgtgga attccgcaat gtcaccttta cttatccggg acgtgacgta cctgcattgc gtaacatcaa cctgaaaatt ccggcaggga agacggttgc tctggttgga cgctctggtt cgggtaaatc aaccatcgcc agcctgatca cgcgttttta cgatattgat gaaggcgaaa tcctgatgga tggtcacgat ctgcgcgagt ataccctggc gtcgttacgt aaccaggttg ctctggtgtc gcagaatgtc catctgttta acgatacggt tgctaacaac attgcttacg cacggactga acagtacagc cgtgagcaaa ttgaagaagc ggcgcgtatg gcctacgcca tggacttcat caataagatg gataacggtc tcgatacagt gattggtgaa aacggcgtgc tgctctctgg cggtcagcgt cagcgtattg ctatcgctcg agccttgttg cgtgatagcc cgattctgat tctggacgaa gctacctcgg ctctggatac cgaatccgaa cgtgcgattc aggcggcact ggatgagttg cagaaaaacc gtacctctct ggtgattgcc caccgcttgt ctaccattga aaaggcagac gaaatcgtgg tcgtcgagga tggtgtcatt gtggaacgcg gtacgcataa cgatttgctt gagcaccgcg gcgtttacgc gcaacttcac aaaatgcagt ttggccaatg ataagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
Details for EscoA.18886.a.TW12.PD38480
PURIFICATION DATe: 7/19/2021
CONCENTRATION: 1.064mg/ml
OBSERVED MW: 72kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20mM tris pH 8.0, 100mM NaCl, 0.06% FA3
EXPRESSION HOST: BL21 DE3
VIAL COUNT (approx.): 14
VIAL VOLUME: 500µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
CID103317, PID9157-1, PUR2991
Validated AA Sequence
MGHHHHHHHH HHSGDYKDDD DKGGSSGGDY KDDDDKGGEN LYFQSHNDKD LSTWQTFRRL WPTIAPFKAG LIVAGVALIL NAASDTFMLS LLKPLLDDGF GKTDRSVLVW MPLVVIGLMI LRGITSYVSS YCISWVSGKV VMTMRRRLFG HMMGMPVSFF DKQSTGTLLS RITYDSEQVA SSSSGALITV VREGASIIGL FIMMFYYSWQ LSIILIVLAP IVSIAIRVVS KRFRNISKNM QNTMGQVTTS AEQMLKGHKE VLIFGGQEVE TKRFDKVSNR MRLQGMKMVS ASSISDPIIQ LIASLALAFV LYAASFPSVM DSLTAGTITV VFSSMIALMR PLKSLTNVNA QFQRGMAACQ TLFTILDSEQ EKDEGKRVIE RATGDVEFRN VTFTYPGRDV PALRNINLKI PAGKTVALVG RSGSGKSTIA SLITRFYDID EGEILMDGHD LREYTLASLR NQVALVSQNV HLFNDTVANN IAYARTEQYS REQIEEAARM AYAMDFINKM DNGLDTVIGE NGVLLSGGQR QRIAIARALL RDSPILILDE ATSALDTESE RAIQAALDEL QKNRTSLVIA HRLSTIEKAD EIVVVEDGVI VERGTHNDLL EHRGVYAQLH KMQFGQ
Validated NT Sequence
atgggccacc atcatcacca tcatcaccac caccacagcg gtgactacaa ggacgatgac gataaaggtg gcagcagcgg tggcgattat aaagatgacg acgataaagg tggcgagaac ctgtatttcc agagccataa cgacaaagat ctctctacgt ggcagacatt ccgccgactg tggccaacca ttgcgccttt caaagcgggt ctgatcgtgg cgggcgtagc gttaatcctc aacgcagcca gcgatacctt catgttatcg ctccttaagc cacttcttga tgatggcttt ggtaaaacag atcgctccgt gctggtgtgg atgccgctgg tggtgatcgg gctgatgatt ttacgtggta tcaccagcta tgtctccagc tactgtatct cctgggtatc aggaaaggtg gtaatgacca tgcgtcgccg cctgtttggt cacatgatgg gaatgccagt ttcattcttt gacaaacagt caacgggtac gctgttgtca cgtattacct acgattccga acaggttgct tcttcttctt ccggcgcact gattactgtt gtgcgtgaag gtgcgtcgat catcggcctg ttcatcatga tgttctatta cagttggcaa ctgtcgatca ttttgattgt gctggcaccg attgtttcga ttgcgattcg cgttgtatcg aagcgttttc gcaacatcag taaaaacatg cagaacacca tggggcaggt gaccaccagc gcagaacaaa tgctgaaggg ccacaaagaa gtattgattt tcggtggtca ggaagtggaa acgaaacgct ttgataaagt cagcaaccga atgcgtcttc aggggatgaa aatggtttca gcctcttcca tctctgatcc gatcattcag ctgatcgcct ctttggcgct ggcgtttgtt ctgtatgcgg cgagcttccc aagtgtcatg gatagcctga ctgccggtac gattaccgtt gttttctctt caatgattgc actgatgcgt ccgctgaaat cgctgaccaa cgttaacgcc cagttccagc gcggtatggc ggcttgtcag acgctgttta ccattctgga cagtgagcag gagaaagatg aaggtaagcg cgtgatcgag cgtgcgactg gcgacgtgga attccgcaat gtcaccttta cttatccggg acgtgacgta cctgcattgc gtaacatcaa cctgaaaatt ccggcaggga agacggttgc tctggttgga cgctctggtt cgggtaaatc aaccatcgcc agcctgatca cgcgttttta cgatattgat gaaggcgaaa tcctgatgga tggtcacgat ctgcgcgagt ataccctggc gtcgttacgt aaccaggttg ctctggtgtc gcagaatgtc catctgttta acgatacggt tgctaacaac attgcttacg cacggactga acagtacagc cgtgagcaaa ttgaagaagc ggcgcgtatg gcctacgcca tggacttcat caataagatg gataacggtc tcgatacagt gattggtgaa aacggcgtgc tgctctctgg cggtcagcgt cagcgtattg ctatcgctcg agccttgttg cgtgatagcc cgattctgat tctggacgaa gctacctcgg ctctggatac cgaatccgaa cgtgcgattc aggcggcact ggatgagttg cagaaaaacc gtacctctct ggtgattgcc caccgcttgt ctaccattga aaaggcagac gaaatcgtgg tcgtcgagga tggtgtcatt gtggaacgcg gtacgcataa cgatttgctt gagcaccgcg gcgtttacgc gcaacttcac aaaatgcagt ttggccaatg ataa
Expressed Protein Sequence
MGHHHHHHHH HHSGDYKDDD DKGGSSGGDY KDDDDKGGEN LYFQSHNDKD LSTWQTFRRL WPTIAPFKAG LIVAGVALIL NAASDTFMLS LLKPLLDDGF GKTDRSVLVW MPLVVIGLMI LRGITSYVSS YCISWVSGKV VMTMRRRLFG HMMGMPVSFF DKQSTGTLLS RITYDSEQVA SSSSGALITV VREGASIIGL FIMMFYYSWQ LSIILIVLAP IVSIAIRVVS KRFRNISKNM QNTMGQVTTS AEQMLKGHKE VLIFGGQEVE TKRFDKVSNR MRLQGMKMVS ASSISDPIIQ LIASLALAFV LYAASFPSVM DSLTAGTITV VFSSMIALMR PLKSLTNVNA QFQRGMAACQ TLFTILDSEQ EKDEGKRVIE RATGDVEFRN VTFTYPGRDV PALRNINLKI PAGKTVALVG RSGSGKSTIA SLITRFYDID EGEILMDGHD LREYTLASLR NQVALVSQNV HLFNDTVANN IAYARTEQYS REQIEEAARM AYAMDFINKM DNGLDTVIGE NGVLLSGGQR QRIAIARALL RDSPILILDE ATSALDTESE RAIQAALDEL QKNRTSLVIA HRLSTIEKAD EIVVVEDGVI VERGTHNDLL EHRGVYAQLH KMQFGQ
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ATCATCACCA TCATCACCAC CACCACAGCG GTGACTACAA GGACGATGAC GATAAAGGTG GCAGCAGCGG TGGCGATTAT AAAGATGACG ACGATAAAGG TGGCGAGAAC CTGTATTTCC AGAGCCATAA CGACAAAGAT CTCTCTACGT GGCAGACATT CCGCCGACTG TGGCCAACCA TTGCGCCTTT CAAAGCGGGT CTGATCGTGG CGGGCGTAGC GTTAATCCTC AACGCAGCCA GCGATACCTT CATGTTATCG CTCCTTAAGC CACTTCTTGA TGATGGCTTT GGTAAAACAG ATCGCTCCGT GCTGGTGTGG ATGCCGCTGG TGGTGATCGG GCTGATGATT TTACGTGGTA TCACCAGCTA TGTCTCCAGC TACTGTATCT CCTGGGTATC AGGAAAGGTG GTAATGACCA TGCGTCGCCG CCTGTTTGGT CACATGATGG GAATGCCAGT TTCATTCTTT GACAAACAGT CAACGGGTAC GCTGTTGTCA CGTATTACCT ACGATTCCGA ACAGGTTGCT TCTTCTTCTT CCGGCGCACT GATTACTGTT GTGCGTGAAG GTGCGTCGAT CATCGGCCTG TTCATCATGA TGTTCTATTA CAGTTGGCAA CTGTCGATCA TTTTGATTGT GCTGGCACCG ATTGTTTCGA TTGCGATTCG CGTTGTATCG AAGCGTTTTC GCAACATCAG TAAAAACATG CAGAACACCA TGGGGCAGGT GACCACCAGC GCAGAACAAA TGCTGAAGGG CCACAAAGAA GTATTGATTT TCGGTGGTCA GGAAGTGGAA ACGAAACGCT TTGATAAAGT CAGCAACCGA ATGCGTCTTC AGGGGATGAA AATGGTTTCA GCCTCTTCCA TCTCTGATCC GATCATTCAG CTGATCGCCT CTTTGGCGCT GGCGTTTGTT CTGTATGCGG CGAGCTTCCC AAGTGTCATG GATAGCCTGA CTGCCGGTAC GATTACCGTT GTTTTCTCTT CAATGATTGC ACTGATGCGT CCGCTGAAAT CGCTGACCAA CGTTAACGCC CAGTTCCAGC GCGGTATGGC GGCTTGTCAG ACGCTGTTTA CCATTCTGGA CAGTGAGCAG GAGAAAGATG AAGGTAAGCG CGTGATCGAG CGTGCGACTG GCGACGTGGA ATTCCGCAAT GTCACCTTTA CTTATCCGGG ACGTGACGTA CCTGCATTGC GTAACATCAA CCTGAAAATT CCGGCAGGGA AGACGGTTGC TCTGGTTGGA CGCTCTGGTT CGGGTAAATC AACCATCGCC AGCCTGATCA CGCGTTTTTA CGATATTGAT GAAGGCGAAA TCCTGATGGA TGGTCACGAT CTGCGCGAGT ATACCCTGGC GTCGTTACGT AACCAGGTTG CTCTGGTGTC GCAGAATGTC CATCTGTTTA ACGATACGGT TGCTAACAAC ATTGCTTACG CACGGACTGA ACAGTACAGC CGTGAGCAAA TTGAAGAAGC GGCGCGTATG GCCTACGCCA TGGACTTCAT CAATAAGATG GATAACGGTC TCGATACAGT GATTGGTGAA AACGGCGTGC TGCTCTCTGG CGGTCAGCGT CAGCGTATTG CTATCGCTCG AGCCTTGTTG CGTGATAGCC CGATTCTGAT TCTGGACGAA GCTACCTCGG CTCTGGATAC CGAATCCGAA CGTGCGATTC AGGCGGCACT GGATGAGTTG CAGAAAAACC GTACCTCTCT GGTGATTGCC CACCGCTTGT CTACCATTGA AAAGGCAGAC GAAATCGTGG TCGTCGAGGA TGGTGTCATT GTGGAACGCG GTACGCATAA CGATTTGCTT GAGCACCGCG GCGTTTACGC GCAACTTCAC AAAATGCAGT TTGGCCAATG ATAAGCTTGC GGCCGCACTC GAGCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT
Details for EscoA.18886.a.TW12.PD38484
PURIFICATION DATe: 9/27/2021
CONCENTRATION: 1.2mg/ml
OBSERVED MW: 72kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25mM HEPES pH 7.5, 150mM NaCl, 0.02% DDM, 200ug/mL FLAG peptide
EXPRESSION HOST: BL21 DE3
VIAL COUNT (approx.): 12
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
CID103317, PID9297-1, PUR3096
Validated AA Sequence
MGHHHHHHHH HHSGDYKDDD DKGGSSGGDY KDDDDKGGEN LYFQSHNDKD LSTWQTFRRL WPTIAPFKAG LIVAGVALIL NAASDTFMLS LLKPLLDDGF GKTDRSVLVW MPLVVIGLMI LRGITSYVSS YCISWVSGKV VMTMRRRLFG HMMGMPVSFF DKQSTGTLLS RITYDSEQVA SSSSGALITV VREGASIIGL FIMMFYYSWQ LSIILIVLAP IVSIAIRVVS KRFRNISKNM QNTMGQVTTS AEQMLKGHKE VLIFGGQEVE TKRFDKVSNR MRLQGMKMVS ASSISDPIIQ LIASLALAFV LYAASFPSVM DSLTAGTITV VFSSMIALMR PLKSLTNVNA QFQRGMAACQ TLFTILDSEQ EKDEGKRVIE RATGDVEFRN VTFTYPGRDV PALRNINLKI PAGKTVALVG RSGSGKSTIA SLITRFYDID EGEILMDGHD LREYTLASLR NQVALVSQNV HLFNDTVANN IAYARTEQYS REQIEEAARM AYAMDFINKM DNGLDTVIGE NGVLLSGGQR QRIAIARALL RDSPILILDE ATSALDTESE RAIQAALDEL QKNRTSLVIA HRLSTIEKAD EIVVVEDGVI VERGTHNDLL EHRGVYAQLH KMQFGQ
Validated NT Sequence
atgggccacc atcatcacca tcatcaccac caccacagcg gtgactacaa ggacgatgac gataaaggtg gcagcagcgg tggcgattat aaagatgacg acgataaagg tggcgagaac ctgtatttcc agagccataa cgacaaagat ctctctacgt ggcagacatt ccgccgactg tggccaacca ttgcgccttt caaagcgggt ctgatcgtgg cgggcgtagc gttaatcctc aacgcagcca gcgatacctt catgttatcg ctccttaagc cacttcttga tgatggcttt ggtaaaacag atcgctccgt gctggtgtgg atgccgctgg tggtgatcgg gctgatgatt ttacgtggta tcaccagcta tgtctccagc tactgtatct cctgggtatc aggaaaggtg gtaatgacca tgcgtcgccg cctgtttggt cacatgatgg gaatgccagt ttcattcttt gacaaacagt caacgggtac gctgttgtca cgtattacct acgattccga acaggttgct tcttcttctt ccggcgcact gattactgtt gtgcgtgaag gtgcgtcgat catcggcctg ttcatcatga tgttctatta cagttggcaa ctgtcgatca ttttgattgt gctggcaccg attgtttcga ttgcgattcg cgttgtatcg aagcgttttc gcaacatcag taaaaacatg cagaacacca tggggcaggt gaccaccagc gcagaacaaa tgctgaaggg ccacaaagaa gtattgattt tcggtggtca ggaagtggaa acgaaacgct ttgataaagt cagcaaccga atgcgtcttc aggggatgaa aatggtttca gcctcttcca tctctgatcc gatcattcag ctgatcgcct ctttggcgct ggcgtttgtt ctgtatgcgg cgagcttccc aagtgtcatg gatagcctga ctgccggtac gattaccgtt gttttctctt caatgattgc actgatgcgt ccgctgaaat cgctgaccaa cgttaacgcc cagttccagc gcggtatggc ggcttgtcag acgctgttta ccattctgga cagtgagcag gagaaagatg aaggtaagcg cgtgatcgag cgtgcgactg gcgacgtgga attccgcaat gtcaccttta cttatccggg acgtgacgta cctgcattgc gtaacatcaa cctgaaaatt ccggcaggga agacggttgc tctggttgga cgctctggtt cgggtaaatc aaccatcgcc agcctgatca cgcgttttta cgatattgat gaaggcgaaa tcctgatgga tggtcacgat ctgcgcgagt ataccctggc gtcgttacgt aaccaggttg ctctggtgtc gcagaatgtc catctgttta acgatacggt tgctaacaac attgcttacg cacggactga acagtacagc cgtgagcaaa ttgaagaagc ggcgcgtatg gcctacgcca tggacttcat caataagatg gataacggtc tcgatacagt gattggtgaa aacggcgtgc tgctctctgg cggtcagcgt cagcgtattg ctatcgctcg agccttgttg cgtgatagcc cgattctgat tctggacgaa gctacctcgg ctctggatac cgaatccgaa cgtgcgattc aggcggcact ggatgagttg cagaaaaacc gtacctctct ggtgattgcc caccgcttgt ctaccattga aaaggcagac gaaatcgtgg tcgtcgagga tggtgtcatt gtggaacgcg gtacgcataa cgatttgctt gagcaccgcg gcgtttacgc gcaacttcac aaaatgcagt ttggccaatg ataa
Expressed Protein Sequence
MGHHHHHHHH HHSGDYKDDD DKGGSSGGDY KDDDDKGGEN LYFQSHNDKD LSTWQTFRRL WPTIAPFKAG LIVAGVALIL NAASDTFMLS LLKPLLDDGF GKTDRSVLVW MPLVVIGLMI LRGITSYVSS YCISWVSGKV VMTMRRRLFG HMMGMPVSFF DKQSTGTLLS RITYDSEQVA SSSSGALITV VREGASIIGL FIMMFYYSWQ LSIILIVLAP IVSIAIRVVS KRFRNISKNM QNTMGQVTTS AEQMLKGHKE VLIFGGQEVE TKRFDKVSNR MRLQGMKMVS ASSISDPIIQ LIASLALAFV LYAASFPSVM DSLTAGTITV VFSSMIALMR PLKSLTNVNA QFQRGMAACQ TLFTILDSEQ EKDEGKRVIE RATGDVEFRN VTFTYPGRDV PALRNINLKI PAGKTVALVG RSGSGKSTIA SLITRFYDID EGEILMDGHD LREYTLASLR NQVALVSQNV HLFNDTVANN IAYARTEQYS REQIEEAARM AYAMDFINKM DNGLDTVIGE NGVLLSGGQR QRIAIARALL RDSPILILDE ATSALDTESE RAIQAALDEL QKNRTSLVIA HRLSTIEKAD EIVVVEDGVI VERGTHNDLL EHRGVYAQLH KMQFGQ
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ATCATCACCA TCATCACCAC CACCACAGCG GTGACTACAA GGACGATGAC GATAAAGGTG GCAGCAGCGG TGGCGATTAT AAAGATGACG ACGATAAAGG TGGCGAGAAC CTGTATTTCC AGAGCCATAA CGACAAAGAT CTCTCTACGT GGCAGACATT CCGCCGACTG TGGCCAACCA TTGCGCCTTT CAAAGCGGGT CTGATCGTGG CGGGCGTAGC GTTAATCCTC AACGCAGCCA GCGATACCTT CATGTTATCG CTCCTTAAGC CACTTCTTGA TGATGGCTTT GGTAAAACAG ATCGCTCCGT GCTGGTGTGG ATGCCGCTGG TGGTGATCGG GCTGATGATT TTACGTGGTA TCACCAGCTA TGTCTCCAGC TACTGTATCT CCTGGGTATC AGGAAAGGTG GTAATGACCA TGCGTCGCCG CCTGTTTGGT CACATGATGG GAATGCCAGT TTCATTCTTT GACAAACAGT CAACGGGTAC GCTGTTGTCA CGTATTACCT ACGATTCCGA ACAGGTTGCT TCTTCTTCTT CCGGCGCACT GATTACTGTT GTGCGTGAAG GTGCGTCGAT CATCGGCCTG TTCATCATGA TGTTCTATTA CAGTTGGCAA CTGTCGATCA TTTTGATTGT GCTGGCACCG ATTGTTTCGA TTGCGATTCG CGTTGTATCG AAGCGTTTTC GCAACATCAG TAAAAACATG CAGAACACCA TGGGGCAGGT GACCACCAGC GCAGAACAAA TGCTGAAGGG CCACAAAGAA GTATTGATTT TCGGTGGTCA GGAAGTGGAA ACGAAACGCT TTGATAAAGT CAGCAACCGA ATGCGTCTTC AGGGGATGAA AATGGTTTCA GCCTCTTCCA TCTCTGATCC GATCATTCAG CTGATCGCCT CTTTGGCGCT GGCGTTTGTT CTGTATGCGG CGAGCTTCCC AAGTGTCATG GATAGCCTGA CTGCCGGTAC GATTACCGTT GTTTTCTCTT CAATGATTGC ACTGATGCGT CCGCTGAAAT CGCTGACCAA CGTTAACGCC CAGTTCCAGC GCGGTATGGC GGCTTGTCAG ACGCTGTTTA CCATTCTGGA CAGTGAGCAG GAGAAAGATG AAGGTAAGCG CGTGATCGAG CGTGCGACTG GCGACGTGGA ATTCCGCAAT GTCACCTTTA CTTATCCGGG ACGTGACGTA CCTGCATTGC GTAACATCAA CCTGAAAATT CCGGCAGGGA AGACGGTTGC TCTGGTTGGA CGCTCTGGTT CGGGTAAATC AACCATCGCC AGCCTGATCA CGCGTTTTTA CGATATTGAT GAAGGCGAAA TCCTGATGGA TGGTCACGAT CTGCGCGAGT ATACCCTGGC GTCGTTACGT AACCAGGTTG CTCTGGTGTC GCAGAATGTC CATCTGTTTA ACGATACGGT TGCTAACAAC ATTGCTTACG CACGGACTGA ACAGTACAGC CGTGAGCAAA TTGAAGAAGC GGCGCGTATG GCCTACGCCA TGGACTTCAT CAATAAGATG GATAACGGTC TCGATACAGT GATTGGTGAA AACGGCGTGC TGCTCTCTGG CGGTCAGCGT CAGCGTATTG CTATCGCTCG AGCCTTGTTG CGTGATAGCC CGATTCTGAT TCTGGACGAA GCTACCTCGG CTCTGGATAC CGAATCCGAA CGTGCGATTC AGGCGGCACT GGATGAGTTG CAGAAAAACC GTACCTCTCT GGTGATTGCC CACCGCTTGT CTACCATTGA AAAGGCAGAC GAAATCGTGG TCGTCGAGGA TGGTGTCATT GTGGAACGCG GTACGCATAA CGATTTGCTT GAGCACCGCG GCGTTTACGC GCAACTTCAC AAAATGCAGT TTGGCCAATG ATAAGCTTGC GGCCGCACTC GAGCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT