CENTER ID: | EscoA.01643.a |
ORGANISM: | Escherichia coli K12 |
ASSOCIATED DISEASE: | Food poisoning |
CURRENT STATUS: | purified |
COMMUNITY REQUEST: | True |
NIH RISK GROUP: | 1 |
SELECT AGENT: | False |
NIH PRIORITY pathogens category: |
IIIB |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
EscoA.01643.a.A3.GE44784 | soluble domain 2 | 327 | 495 | ||
EscoA.01643.a.AE3.GE44785 | soluble domain 2 | 327 | 495 | ||
EscoA.01643.a.HZ3.GE44786 | soluble domain 2 | 327 | 495 |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
EscoA.01643.a.HZ3.PW39197 | soluble domain 2 | 327 | 495 |
RESOURCE | REFERENCE ID |
---|---|
BV-BRC: | fig|1411691.4.peg.2195 |
UniProt: | P22188 |
HARVESTED ON: | 8/12/2022 |
SEQUENCED ON: | 7/28/2022 |
EXPECTED MW: | 20kDa |
OBSERVED MW: | 20kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Low Expression |
SOLUBLE EXPRESSION LEVEL | Insoluble |
EXPRESSION HOST: | Rosetta2 (DE3) |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |
HARVESTED ON: | 8/12/2022 |
SEQUENCED ON: | 7/28/2022 |
EXPECTED MW: | 19kDa |
OBSERVED MW: | 19kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Low Expression |
SOLUBLE EXPRESSION LEVEL | Insoluble |
EXPRESSION HOST: | Rosetta2 (DE3) |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |
HARVESTED ON: | 8/12/2022 |
SEQUENCED ON: | 7/28/2022 |
EXPECTED MW: | 32kDa |
OBSERVED MW: | 32kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | Moderate Expression |
EXPRESSION HOST: | Rosetta2 (DE3) |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |
PURIFICATION DATe: | 3/8/2023 |
CONCENTRATION: | 16.5mg/ml |
OBSERVED MW: | 32kDa |
EXPRESSION LEVEL: | Low Expression |
PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
EXPRESSION HOST: | Rosetta2 (DE3) |
VIAL COUNT (approx.): | 16 |
VIAL VOLUME: | 110µl |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |