CENTER ID: | EscoA.00166.a |
ORGANISM: | Escherichia coli K12 |
ASSOCIATED DISEASE: | Food poisoning |
CURRENT STATUS: | purified |
COMMUNITY REQUEST: | True |
NIH RISK GROUP: | 1 |
SELECT AGENT: | False |
NIH PRIORITY pathogens category: |
IIIB |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
EscoA.00166.a.D11.GE42001 | full length | 1 | 300 | ||
EscoA.00166.a.JA11.GE41538 | full length | 1 | 305 | ||
EscoA.00166.a.JA12.GE41539 | full length | 1 | 305 |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
EscoA.00166.a.W11.PD00443 | full length | 1 | 306 | ||
EscoA.00166.a.D11.PD38288 | full length | 1 | 300 |
RESOURCE | REFERENCE ID |
---|---|
BV-BRC: | fig|511145.12.peg.100 |
RefSeq: | WP_000595482.1 |
UniProt: | P0A725 |
HARVESTED ON: | 8/16/2017 |
SEQUENCED ON: | 10/25/2017 |
EXPECTED MW: | 45kDa |
OBSERVED MW: | 45kDa |
ANTIBIOTIC MARKER: | kanamycin |
COUNT OF EXPRESSION COLONIES: | Data Unavailable |
TOTAL EXPRESSION LEVEL: | High Expression |
SOLUBLE EXPRESSION LEVEL | High Expression |
EXPRESSION HOST: | BL21 DE3 |
SEQUENCING RESULT: | pass |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |
HARVESTED ON: | 4/12/2017 |
SEQUENCED ON: | 10/25/2017 |
EXPECTED MW: | 36kDa |
OBSERVED MW: | 36kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | Moderate Expression |
EXPRESSION HOST: | BL21 DE3 |
SEQUENCING RESULT: | pass |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |
HARVESTED ON: | 4/20/2017 |
SEQUENCED ON: | 10/25/2017 |
EXPECTED MW: | 36kDa |
OBSERVED MW: | 36kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | High Expression |
SOLUBLE EXPRESSION LEVEL | No Expression |
EXPRESSION HOST: | BL21 DE3 |
SEQUENCING RESULT: | pass |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 93 |
PURIFICATION DATe: | 4/13/2016 |
CONCENTRATION: | 8mg/ml |
OBSERVED MW: | 45kDa |
EXPRESSION LEVEL: | Moderate Expression |
PROTEIN PURIFICATION BUFFER: | 20 mM HEPES pH 7.5, 300 mM NaCl, 2 mM TCEP, 5% glycerol, 0.5 mM ZnCl2 |
EXPRESSION HOST: | data unavailable |
VIAL COUNT (approx.): | 12 |
VIAL VOLUME: | 100µl |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |
PURIFICATION DATe: | 11/28/2017 |
CONCENTRATION: | 10.9mg/ml |
OBSERVED MW: | 45kDa |
EXPRESSION LEVEL: | Low Expression |
PROTEIN PURIFICATION BUFFER: | 25mM HEPES pH 7.0, 100 KCl, 0.1mM ZnSO4, 2mM DTT |
EXPRESSION HOST: | BL21 DE3 |
VIAL COUNT (approx.): | 4 |
VIAL VOLUME: | 50µl |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |