EscoA.00137.a

UDP-N-acetylmuramate--L-alanine ligase (MurC)

CENTER ID: EscoA.00137.a
ORGANISM: Escherichia coli K12
ASSOCIATED DISEASE: Food poisoning
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
EscoA.00137.a.A1.GE44779 full length 1 491
EscoA.00137.a.AE1.GE44780 full length 1 491
EscoA.00137.a.HZ1.GE44781 full length 1 491
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
EscoA.00137.a.A1.PW39150 full length 1 491
EscoA.00137.a.AE1.PW39151 full length 1 491
EscoA.00137.a.HZ1.PW39198 full length 1 491

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|511145.12.peg.95
UniProt: P17952

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNTQQLAKLR SIVPEMRRVR HIHFVGIGGA GMGGIAEVLA NEGYQISGSD LAPNPVTQQL MNLGATIYFN HRPENVRDAS VVVVSSAISA DNPEIVAAHE ARIPVIRRAE MLAELMRFRH GIAIAGTHGK TTTTAMVSSI YAEAGLDPTF VNGGLVKAAG VHARLGHGRY LIAEADESDA SFLHLQPMVA IVTNIEADHM DTYQGDFENL KQTFINFLHN LPFYGRAVMC VDDPVIRELL PRVGRQTTTY GFSEDADVRV EDYQQIGPQG HFTLLRQDKE PMRVTLNAPG RHNALNAAAA VAVATEEGID DEAILRALES FQGTGRRFDF LGEFPLEPVN GKSGTAMLVD DYGHHPTEVD ATIKAARAGW PDKNLVMLFQ PHRFTRTRDL YDDFANVLTQ VDTLLMLEVY PAGEAPIPGA DSRSLCRTIR GRGKIDPILV PDPARVAEML APVLTGNDLI LVQGAGNIGK IARSLAEIKL KPQTPEEEQH D
NT Sequence
atgaatacac aacaattggc aaaactgcgt tccatcgtgc ccgaaatgcg tcgcgttcgg cacatacatt ttgtcggcat tggtggtgcc ggtatgggcg gtattgccga agttctggcc aatgaaggtt atcagatcag tggttccgat ttagcgccaa atccggtcac gcagcagtta atgaatctgg gtgcgacgat ttatttcaac catcgcccgg aaaacgtacg tgatgccagc gtggtcgttg tttccagcgc gatttctgcc gataacccgg aaattgtcgc cgctcatgaa gcgcgtattc cggtgatccg tcgtgccgaa atgctggctg agttaatgcg ttttcgtcat ggcatcgcca ttgccggaac gcacggcaaa acgacaacca ccgcgatggt ttccagcatc tacgcagaag cggggctcga cccaaccttc gttaacggcg ggctggtaaa agcggcgggg gttcatgcgc gtttggggca tggtcggtac ctgattgccg aagcagatga gagtgatgca tcgttcctgc atctgcaacc gatggtggcg attgtcacca atatcgaagc cgaccacatg gatacctacc agggcgactt tgagaattta aaacagactt ttattaattt tctgcacaac ctgccgtttt acggtcgtgc ggtgatgtgt gttgatgatc cggtgatccg cgaattgtta ccgcgagtgg ggcgtcagac cacgacttac ggcttcagcg aagatgccga cgtgcgtgta gaagattatc agcagattgg cccgcagggg cactttacgc tgctgcgcca ggacaaagag ccgatgcgcg tcaccctgaa tgcgccaggt cgtcataacg cgctgaacgc cgcagctgcg gttgcggttg ctacggaaga gggcattgac gacgaggcta ttttgcgggc gcttgaaagc ttccagggga ctggtcgccg ttttgatttc ctcggtgaat tcccgctgga gccagtgaat ggtaaaagcg gtacggcaat gctggtcgat gactacggcc accacccgac ggaagtggac gccaccatta aagcggcgcg cgcaggctgg ccggataaaa acctggtaat gctgtttcag ccgcaccgtt ttacccgtac gcgcgacctg tatgatgatt tcgccaatgt gctgacgcag gttgataccc tgttgatgct ggaagtgtat ccggctggcg aagcgccaat tccgggagcg gacagccgtt cgctgtgtcg cacaattcgt ggacgtggga aaattgatcc cattctggtg ccggatccgg cgcgggtagc cgagatgctg gcaccggtat taaccggtaa cgacctgatt ctcgttcagg gggctggtaa tattggaaaa attgcccgtt ctttagctga aatcaaactg aagccgcaaa ctccggagga agaacaacat gactga
Details for EscoA.00137.a.A1.GE44779
HARVESTED ON: 8/12/2022
SEQUENCED ON: 7/28/2022
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNTQQLAKL RSIVPEMRRV RHIHFVGIGG AGMGGIAEVL ANEGYQISGS DLAPNPVTQQ LMNLGATIYF NHRPENVRDA SVVVVSSAIS ADNPEIVAAH EARIPVIRRA EMLAELMRFR HGIAIAGTHG KTTTTAMVSS IYAEAGLDPT FVNGGLVKAA GVHARLGHGR YLIAEADESD ASFLHLQPMV AIVTNIEADH MDTYQGDFEN LKQTFINFLH NLPFYGRAVM CVDDPVIREL LPRVGRQTTT YGFSEDADVR VEDYQQIGPQ GHFTLLRQDK EPMRVTLNAP GRHNALNAAA AVAVATEEGI DDEAILRALE SFQGTGRRFD FLGEFPLEPV NGKSGTAMLV DDYGHHPTEV DATIKAARAG WPDKNLVMLF QPHRFTRTRD LYDDFANVLT QVDTLLMLEV YPAGEAPIPG ADSRSLCRTI RGRGKIDPIL VPDPARVAEM LAPVLTGNDL ILVQGAGNIG KIARSLAEIK LKPQTPEEEQ HD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaata cacaacaatt ggcaaaactg cgttccatcg tgcccgaaat gcgtcgcgtt cggcacatac attttgtcgg cattggtggt gccggtatgg gcggtattgc cgaagttctg gccaatgaag gttatcagat cagtggttcc gatttagcgc caaatccggt cacgcagcag ttaatgaatc tgggtgcgac gatttatttc aaccatcgcc cggaaaacgt acgtgatgcc agcgtggtcg ttgtttccag cgcgatttct gccgataacc cggaaattgt cgccgctcat gaagcgcgta ttccggtgat ccgtcgtgcc gaaatgctgg ctgagttaat gcgttttcgt catggcatcg ccattgccgg aacgcacggc aaaacgacaa ccaccgcgat ggtttccagc atctacgcag aagcggggct cgacccaacc ttcgttaacg gcgggctggt aaaagcggcg ggggttcatg cgcgtttggg gcatggtcgg tacctgattg ccgaagcaga tgagagtgat gcatcgttcc tgcatctgca accgatggtg gcgattgtca ccaatatcga agccgaccac atggatacct accagggcga ctttgagaat ttaaaacaga cttttattaa ttttctgcac aacctgccgt tttacggtcg tgcggtgatg tgtgttgatg atccggtgat ccgcgaattg ttaccgcgag tggggcgtca gaccacgact tacggcttca gcgaagatgc cgacgtgcgt gtagaagatt atcagcagat tggcccgcag gggcacttta cgctgctgcg ccaggacaaa gagccgatgc gcgtcaccct gaatgcgcca ggtcgtcata acgcgctgaa cgccgcagct gcggttgcgg ttgctacgga agagggcatt gacgacgagg ctattttgcg ggcgcttgaa agcttccagg ggactggtcg ccgttttgat ttcctcggtg aattcccgct ggagccagtg aatggtaaaa gcggtacggc aatgctggtc gatgactacg gccaccaccc gacggaagtg gacgccacca ttaaagcggc gcgcgcaggc tggccggata aaaacctggt aatgctgttt cagccgcacc gttttacccg tacgcgcgac ctgtatgatg atttcgccaa tgtgctgacg caggttgata ccctgttgat gctggaagtg tatccggctg gcgaagcgcc aattccggga gcggacagcc gttcgctgtg tcgcacaatt cgtggacgtg ggaaaattga tcccattctg gtgccggatc cggcgcgggt agccgagatg ctggcaccgg tattaaccgg taacgacctg attctcgttc agggggctgg taatattgga aaaattgccc gttctttagc tgaaatcaaa ctgaagccgc aaactccgga ggaagaacaa catgactaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNTQQLAKL RSIVPEMRRV RHIHFVGIGG AGMGGIAEVL ANEGYQISGS DLAPNPVTQQ LMNLGATIYF NHRPENVRDA SVVVVSSAIS ADNPEIVAAH EARIPVIRRA EMLAELMRFR HGIAIAGTHG KTTTTAMVSS IYAEAGLDPT FVNGGLVKAA GVHARLGHGR YLIAEADESD ASFLHLQPMV AIVTNIEADH MDTYQGDFEN LKQTFINFLH NLPFYGRAVM CVDDPVIREL LPRVGRQTTT YGFSEDADVR VEDYQQIGPQ GHFTLLRQDK EPMRVTLNAP GRHNALNAAA AVAVATEEGI DDEAILRALE SFQGTGRRFD FLGEFPLEPV NGKSGTAMLV DDYGHHPTEV DATIKAARAG WPDKNLVMLF QPHRFTRTRD LYDDFANVLT QVDTLLMLEV YPAGEAPIPG ADSRSLCRTI RGRGKIDPIL VPDPARVAEM LAPVLTGNDL ILVQGAGNIG KIARSLAEIK LKPQTPEEEQ HD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatacacaa caattggcaa aactgcgttc catcgtgccc gaaatgcgtc gcgttcggca catacatttt gtcggcattg gtggtgccgg tatgggcggt attgccgaag ttctggccaa tgaaggttat cagatcagtg gttccgattt agcgccaaat ccggtcacgc agcagttaat gaatctgggt gcgacgattt atttcaacca tcgcccggaa aacgtacgtg atgccagcgt ggtcgttgtt tccagcgcga tttctgccga taacccggaa attgtcgccg ctcatgaagc gcgtattccg gtgatccgtc gtgccgaaat gctggctgag ttaatgcgtt ttcgtcatgg catcgccatt gccggaacgc acggcaaaac gacaaccacc gcgatggttt ccagcatcta cgcagaagcg gggctcgacc caaccttcgt taacggcggg ctggtaaaag cggcgggggt tcatgcgcgt ttggggcatg gtcggtacct gattgccgaa gcagatgaga gtgatgcatc gttcctgcat ctgcaaccga tggtggcgat tgtcaccaat atcgaagccg accacatgga tacctaccag ggcgactttg agaatttaaa acagactttt attaattttc tgcacaacct gccgttttac ggtcgtgcgg tgatgtgtgt tgatgatccg gtgatccgcg aattgttacc gcgagtgggg cgtcagacca cgacttacgg cttcagcgaa gatgccgacg tgcgtgtaga agattatcag cagattggcc cgcaggggca ctttacgctg ctgcgccagg acaaagagcc gatgcgcgtc accctgaatg cgccaggtcg tcataacgcg ctgaacgccg cagctgcggt tgcggttgct acggaagagg gcattgacga cgaggctatt ttgcgggcgc ttgaaagctt ccaggggact ggtcgccgtt ttgatttcct cggtgaattc ccgctggagc cagtgaatgg taaaagcggt acggcaatgc tggtcgatga ctacggccac cacccgacgg aagtggacgc caccattaaa gcggcgcgcg caggctggcc ggataaaaac ctggtaatgc tgtttcagcc gcaccgtttt acccgtacgc gcgacctgta tgatgatttc gccaatgtgc tgacgcaggt tgataccctg ttgatgctgg aagtgtatcc ggctggcgaa gcgccaattc cgggagcgga cagccgttcg ctgtgtcgca caattcgtgg acgtgggaaa attgatccca ttctggtgcc ggatccggcg cgggtagccg agatgctggc accggtatta accggtaacg acctgattct cgttcagggg gctggtaata ttggaaaaat tgcccgttct ttagctgaaa tcaaactgaa gccgcaaact ccggaggaag aacaacatga ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for EscoA.00137.a.AE1.GE44780
HARVESTED ON: 8/12/2022
SEQUENCED ON: 7/28/2022
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MNTQQLAKLR SIVPEMRRVR HIHFVGIGGA GMGGIAEVLA NEGYQISGSD LAPNPVTQQL MNLGATIYFN HRPENVRDAS VVVVSSAISA DNPEIVAAHE ARIPVIRRAE MLAELMRFRH GIAIAGTHGK TTTTAMVSSI YAEAGLDPTF VNGGLVKAAG VHARLGHGRY LIAEADESDA SFLHLQPMVA IVTNIEADHM DTYQGDFENL KQTFINFLHN LPFYGRAVMC VDDPVIRELL PRVGRQTTTY GFSEDADVRV EDYQQIGPQG HFTLLRQDKE PMRVTLNAPG RHNALNAAAA VAVATEEGID DEAILRALES FQGTGRRFDF LGEFPLEPVN GKSGTAMLVD DYGHHPTEVD ATIKAARAGW PDKNLVMLFQ PHRFTRTRDL YDDFANVLTQ VDTLLMLEVY PAGEAPIPGA DSRSLCRTIR GRGKIDPILV PDPARVAEML APVLTGNDLI LVQGAGNIGK IARSLAEIKL KPQTPEEEQH DAGHHHHHH
Validated NT Sequence
atgaatacac aacaattggc aaaactgcgt tccatcgtgc ccgaaatgcg tcgcgttcgg cacatacatt ttgtcggcat tggtggtgcc ggtatgggcg gtattgccga agttctggcc aatgaaggtt atcagatcag tggttccgat ttagcgccaa atccggtcac gcagcagtta atgaatctgg gtgcgacgat ttatttcaac catcgcccgg aaaacgtacg tgatgccagc gtggtcgttg tttccagcgc gatttctgcc gataacccgg aaattgtcgc cgctcatgaa gcgcgtattc cggtgatccg tcgtgccgaa atgctggctg agttaatgcg ttttcgtcat ggcatcgcca ttgccggaac gcacggcaaa acgacaacca ccgcgatggt ttccagcatc tacgcagaag cggggctcga cccaaccttc gttaacggcg ggctggtaaa agcggcgggg gttcatgcgc gtttggggca tggtcggtac ctgattgccg aagcagatga gagtgatgca tcgttcctgc atctgcaacc gatggtggcg attgtcacca atatcgaagc cgaccacatg gatacctacc agggcgactt tgagaattta aaacagactt ttattaattt tctgcacaac ctgccgtttt acggtcgtgc ggtgatgtgt gttgatgatc cggtgatccg cgaattgtta ccgcgagtgg ggcgtcagac cacgacttac ggcttcagcg aagatgccga cgtgcgtgta gaagattatc agcagattgg cccgcagggg cactttacgc tgctgcgcca ggacaaagag ccgatgcgcg tcaccctgaa tgcgccaggt cgtcataacg cgctgaacgc cgcagctgcg gttgcggttg ctacggaaga gggcattgac gacgaggcta ttttgcgggc gcttgaaagc ttccagggga ctggtcgccg ttttgatttc ctcggtgaat tcccgctgga gccagtgaat ggtaaaagcg gtacggcaat gctggtcgat gactacggcc accacccgac ggaagtggac gccaccatta aagcggcgcg cgcaggctgg ccggataaaa acctggtaat gctgtttcag ccgcaccgtt ttacccgtac gcgcgacctg tatgatgatt tcgccaatgt gctgacgcag gttgataccc tgttgatgct ggaagtgtat ccggctggcg aagcgccaat tccgggagcg gacagccgtt cgctgtgtcg cacaattcgt ggacgtggga aaattgatcc cattctggtg ccggatccgg cgcgggtagc cgagatgctg gcaccggtat taaccggtaa cgacctgatt ctcgttcagg gggctggtaa tattggaaaa attgcccgtt ctttagctga aatcaaactg aagccgcaaa ctccggagga agaacaacat gacgctgggc accaccatca tcatcattaa
Expected Protein Sequence
MNTQQLAKLR SIVPEMRRVR HIHFVGIGGA GMGGIAEVLA NEGYQISGSD LAPNPVTQQL MNLGATIYFN HRPENVRDAS VVVVSSAISA DNPEIVAAHE ARIPVIRRAE MLAELMRFRH GIAIAGTHGK TTTTAMVSSI YAEAGLDPTF VNGGLVKAAG VHARLGHGRY LIAEADESDA SFLHLQPMVA IVTNIEADHM DTYQGDFENL KQTFINFLHN LPFYGRAVMC VDDPVIRELL PRVGRQTTTY GFSEDADVRV EDYQQIGPQG HFTLLRQDKE PMRVTLNAPG RHNALNAAAA VAVATEEGID DEAILRALES FQGTGRRFDF LGEFPLEPVN GKSGTAMLVD DYGHHPTEVD ATIKAARAGW PDKNLVMLFQ PHRFTRTRDL YDDFANVLTQ VDTLLMLEVY PAGEAPIPGA DSRSLCRTIR GRGKIDPILV PDPARVAEML APVLTGNDLI LVQGAGNIGK IARSLAEIKL KPQTPEEEQH DGHHHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gaatacacaa caattggcaa aactgcgttc catcgtgccc gaaatgcgtc gcgttcggca catacatttt gtcggcattg gtggtgccgg tatgggcggt attgccgaag ttctggccaa tgaaggttat cagatcagtg gttccgattt agcgccaaat ccggtcacgc agcagttaat gaatctgggt gcgacgattt atttcaacca tcgcccggaa aacgtacgtg atgccagcgt ggtcgttgtt tccagcgcga tttctgccga taacccggaa attgtcgccg ctcatgaagc gcgtattccg gtgatccgtc gtgccgaaat gctggctgag ttaatgcgtt ttcgtcatgg catcgccatt gccggaacgc acggcaaaac gacaaccacc gcgatggttt ccagcatcta cgcagaagcg gggctcgacc caaccttcgt taacggcggg ctggtaaaag cggcgggggt tcatgcgcgt ttggggcatg gtcggtacct gattgccgaa gcagatgaga gtgatgcatc gttcctgcat ctgcaaccga tggtggcgat tgtcaccaat atcgaagccg accacatgga tacctaccag ggcgactttg agaatttaaa acagactttt attaattttc tgcacaacct gccgttttac ggtcgtgcgg tgatgtgtgt tgatgatccg gtgatccgcg aattgttacc gcgagtgggg cgtcagacca cgacttacgg cttcagcgaa gatgccgacg tgcgtgtaga agattatcag cagattggcc cgcaggggca ctttacgctg ctgcgccagg acaaagagcc gatgcgcgtc accctgaatg cgccaggtcg tcataacgcg ctgaacgccg cagctgcggt tgcggttgct acggaagagg gcattgacga cgaggctatt ttgcgggcgc ttgaaagctt ccaggggact ggtcgccgtt ttgatttcct cggtgaattc ccgctggagc cagtgaatgg taaaagcggt acggcaatgc tggtcgatga ctacggccac cacccgacgg aagtggacgc caccattaaa gcggcgcgcg caggctggcc ggataaaaac ctggtaatgc tgtttcagcc gcaccgtttt acccgtacgc gcgacctgta tgatgatttc gccaatgtgc tgacgcaggt tgataccctg ttgatgctgg aagtgtatcc ggctggcgaa gcgccaattc cgggagcgga cagccgttcg ctgtgtcgca caattcgtgg acgtgggaaa attgatccca ttctggtgcc ggatccggcg cgggtagccg agatgctggc accggtatta accggtaacg acctgattct cgttcagggg gctggtaata ttggaaaaat tgcccgttct ttagctgaaa tcaaactgaa gccgcaaact ccggaggaag aacaacatga cgggcaccac catcatcatc attaacggat ccgaattcga gctccgtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat
Details for EscoA.00137.a.HZ1.GE44781
HARVESTED ON: 8/12/2022
SEQUENCED ON: 7/28/2022
EXPECTED MW: 67kDa
OBSERVED MW: 67kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMN TQQLAKLRSI VPEMRRVRHI HFVGIGGAGM GGIAEVLANE GYQISGSDLA PNPVTQQLMN LGATIYFNHR PENVRDASVV VVSSAISADN PEIVAAHEAR IPVIRRAEML AELMRFRHGI AIAGTHGKTT TTAMVSSIYA EAGLDPTFVN GGLVKAAGVH ARLGHGRYLI AEADESDASF LHLQPMVAIV TNIEADHMDT YQGDFENLKQ TFINFLHNLP FYGRAVMCVD DPVIRELLPR VGRQTTTYGF SEDADVRVED YQQIGPQGHF TLLRQDKEPM RVTLNAPGRH NALNAAAAVA VATEEGIDDE AILRALESFQ GTGRRFDFLG EFPLEPVNGK SGTAMLVDDY GHHPTEVDAT IKAARAGWPD KNLVMLFQPH RFTRTRDLYD DFANVLTQVD TLLMLEVYPA GEAPIPGADS RSLCRTIRGR GKIDPILVPD PAQVAEMLAP VLTGNDLILV QGAGNIGKIA RSLAEIKLKP QTPEEEQHD
Validated NT Sequence
atggctcatc accatcacca tcatatgaag tcggactcag aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg ataacgatat tattgaggct cacagagaac agattggtgg taccctggaa gctcagaccc agggtcctgg ttcgatgaat acacaacaat tggcaaaact gcgttccatc gtgcccgaaa tgcgtcgcgt tcggcacata cattttgtcg gcattggtgg tgccggtatg ggcggtattg ccgaagttct ggccaatgaa ggttatcaga tcagtggttc cgatttagcg ccaaatccgg tcacgcagca gttaatgaat ctgggagcga cgatttattt caaccatcgc ccggaaaacg tacgtgatgc cagcgtggtc gttgtttcca gcgcgatttc tgccgataac ccggaaattg tcgcagctca tgaagcgcgt attccggtga tccgtcgtgc cgaaatgctg gctgagttaa tgcgttttcg tcatggcatc gccattgcag gaacgcacgg caaaacgaca accaccgcga tggtttccag catctacgca gaagcggggc tcgacccaac cttcgttaac ggcgggctgg taaaagcggc gggggttcat gcgcgtttgg ggcatggtcg gtacctgatt gccgaagcag atgagagtga tgcatcgttc ctgcatctgc aaccgatggt ggcgattgtc accaatatcg aagccgacca catggatacc taccagggcg actttgagaa tttaaaacag acttttatta attttctgca caacctgcct ttttacggtc gtgcggtgat gtgtgttgat gatccggtga tccgcgaatt gttaccgcga gtgggacgtc agaccacgac ttacggcttc agcgaagatg ccgacgtgcg tgtagaagat tatcagcaga ttggcccgca ggggcacttt acgctgctgc gccaggacaa agagccgatg cgcgtcaccc tgaatgcgcc aggtcgtcat aacgcgctga acgccgcagc tgcggttgcg gttgctacgg aagagggaat tgatgacgag gctattttac gtgcgctgga gagcttccag gggactgggc gccgttttga tttcctcggt gaattcccgc tggagccagt gaatggtaaa agcggtacgg cgatgttggt cgatgactac ggccaccacc cgacggaagt ggacgccact attaaagcgg cgcgtgcagg ctggccggat aaaaacctgg taatgctgtt tcagccgcac cgttttaccc gtacgcgcga cctgtatgat gatttcgcca atgtgctgac gcaggttgat accctgttga tgctggaagt gtatccggct ggcgaagcgc caattccggg agcggacagc cgttcgctgt gtcgcacaat tcgtggacgt gggaaaattg atcccattct ggtgccggac cctgcgcagg tagccgagat gctggcaccg gtattaaccg gtaacgacct gattctcgtt cagggggctg gtaatatcgg aaaaattgcc cgttctttag ctgaaatcaa actgaagccg caaactccgg aggaagaaca acatgactaa taa
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMN TQQLAKLRSI VPEMRRVRHI HFVGIGGAGM GGIAEVLANE GYQISGSDLA PNPVTQQLMN LGATIYFNHR PENVRDASVV VVSSAISADN PEIVAAHEAR IPVIRRAEML AELMRFRHGI AIAGTHGKTT TTAMVSSIYA EAGLDPTFVN GGLVKAAGVH ARLGHGRYLI AEADESDASF LHLQPMVAIV TNIEADHMDT YQGDFENLKQ TFINFLHNLP FYGRAVMCVD DPVIRELLPR VGRQTTTYGF SEDADVRVED YQQIGPQGHF TLLRQDKEPM RVTLNAPGRH NALNAAAAVA VATEEGIDDE AILRALESFQ GTGRRFDFLG EFPLEPVNGK SGTAMLVDDY GHHPTEVDAT IKAARAGWPD KNLVMLFQPH RFTRTRDLYD DFANVLTQVD TLLMLEVYPA GEAPIPGADS RSLCRTIRGR GKIDPILVPD PARVAEMLAP VLTGNDLILV QGAGNIGKIA RSLAEIKLKP QTPEEEQHD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tgaatacaca acaattggca aaactgcgtt ccatcgtgcc cgaaatgcgt cgcgttcggc acatacattt tgtcggcatt ggtggtgccg gtatgggcgg tattgccgaa gttctggcca atgaaggtta tcagatcagt ggttccgatt tagcgccaaa tccggtcacg cagcagttaa tgaatctggg tgcgacgatt tatttcaacc atcgcccgga aaacgtacgt gatgccagcg tggtcgttgt ttccagcgcg atttctgccg ataacccgga aattgtcgcc gctcatgaag cgcgtattcc ggtgatccgt cgtgccgaaa tgctggctga gttaatgcgt tttcgtcatg gcatcgccat tgccggaacg cacggcaaaa cgacaaccac cgcgatggtt tccagcatct acgcagaagc ggggctcgac ccaaccttcg ttaacggcgg gctggtaaaa gcggcggggg ttcatgcgcg tttggggcat ggtcggtacc tgattgccga agcagatgag agtgatgcat cgttcctgca tctgcaaccg atggtggcga ttgtcaccaa tatcgaagcc gaccacatgg atacctacca gggcgacttt gagaatttaa aacagacttt tattaatttt ctgcacaacc tgccgtttta cggtcgtgcg gtgatgtgtg ttgatgatcc ggtgatccgc gaattgttac cgcgagtggg gcgtcagacc acgacttacg gcttcagcga agatgccgac gtgcgtgtag aagattatca gcagattggc ccgcaggggc actttacgct gctgcgccag gacaaagagc cgatgcgcgt caccctgaat gcgccaggtc gtcataacgc gctgaacgcc gcagctgcgg ttgcggttgc tacggaagag ggcattgacg acgaggctat tttgcgggcg cttgaaagct tccaggggac tggtcgccgt tttgatttcc tcggtgaatt cccgctggag ccagtgaatg gtaaaagcgg tacggcaatg ctggtcgatg actacggcca ccacccgacg gaagtggacg ccaccattaa agcggcgcgc gcaggctggc cggataaaaa cctggtaatg ctgtttcagc cgcaccgttt tacccgtacg cgcgacctgt atgatgattt cgccaatgtg ctgacgcagg ttgataccct gttgatgctg gaagtgtatc cggctggcga agcgccaatt ccgggagcgg acagccgttc gctgtgtcgc acaattcgtg gacgtgggaa aattgatccc attctggtgc cggatccggc gcgggtagcc gagatgctgg caccggtatt aaccggtaac gacctgattc tcgttcaggg ggctggtaat attggaaaaa ttgcccgttc tttagctgaa atcaaactga agccgcaaac tccggaggaa gaacaacatg actaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for EscoA.00137.a.A1.PW39150
PURIFICATION DATe: 9/21/2022
CONCENTRATION: 11.3mg/ml
OBSERVED MW: 56kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: Rosetta2 (DE3)
VIAL COUNT (approx.): 5
VIAL VOLUME: 55µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNTQQLAKL RSIVPEMRRV RHIHFVGIGG AGMGGIAEVL ANEGYQISGS DLAPNPVTQQ LMNLGATIYF NHRPENVRDA SVVVVSSAIS ADNPEIVAAH EARIPVIRRA EMLAELMRFR HGIAIAGTHG KTTTTAMVSS IYAEAGLDPT FVNGGLVKAA GVHARLGHGR YLIAEADESD ASFLHLQPMV AIVTNIEADH MDTYQGDFEN LKQTFINFLH NLPFYGRAVM CVDDPVIREL LPRVGRQTTT YGFSEDADVR VEDYQQIGPQ GHFTLLRQDK EPMRVTLNAP GRHNALNAAA AVAVATEEGI DDEAILRALE SFQGTGRRFD FLGEFPLEPV NGKSGTAMLV DDYGHHPTEV DATIKAARAG WPDKNLVMLF QPHRFTRTRD LYDDFANVLT QVDTLLMLEV YPAGEAPIPG ADSRSLCRTI RGRGKIDPIL VPDPARVAEM LAPVLTGNDL ILVQGAGNIG KIARSLAEIK LKPQTPEEEQ HD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaata cacaacaatt ggcaaaactg cgttccatcg tgcccgaaat gcgtcgcgtt cggcacatac attttgtcgg cattggtggt gccggtatgg gcggtattgc cgaagttctg gccaatgaag gttatcagat cagtggttcc gatttagcgc caaatccggt cacgcagcag ttaatgaatc tgggtgcgac gatttatttc aaccatcgcc cggaaaacgt acgtgatgcc agcgtggtcg ttgtttccag cgcgatttct gccgataacc cggaaattgt cgccgctcat gaagcgcgta ttccggtgat ccgtcgtgcc gaaatgctgg ctgagttaat gcgttttcgt catggcatcg ccattgccgg aacgcacggc aaaacgacaa ccaccgcgat ggtttccagc atctacgcag aagcggggct cgacccaacc ttcgttaacg gcgggctggt aaaagcggcg ggggttcatg cgcgtttggg gcatggtcgg tacctgattg ccgaagcaga tgagagtgat gcatcgttcc tgcatctgca accgatggtg gcgattgtca ccaatatcga agccgaccac atggatacct accagggcga ctttgagaat ttaaaacaga cttttattaa ttttctgcac aacctgccgt tttacggtcg tgcggtgatg tgtgttgatg atccggtgat ccgcgaattg ttaccgcgag tggggcgtca gaccacgact tacggcttca gcgaagatgc cgacgtgcgt gtagaagatt atcagcagat tggcccgcag gggcacttta cgctgctgcg ccaggacaaa gagccgatgc gcgtcaccct gaatgcgcca ggtcgtcata acgcgctgaa cgccgcagct gcggttgcgg ttgctacgga agagggcatt gacgacgagg ctattttgcg ggcgcttgaa agcttccagg ggactggtcg ccgttttgat ttcctcggtg aattcccgct ggagccagtg aatggtaaaa gcggtacggc aatgctggtc gatgactacg gccaccaccc gacggaagtg gacgccacca ttaaagcggc gcgcgcaggc tggccggata aaaacctggt aatgctgttt cagccgcacc gttttacccg tacgcgcgac ctgtatgatg atttcgccaa tgtgctgacg caggttgata ccctgttgat gctggaagtg tatccggctg gcgaagcgcc aattccggga gcggacagcc gttcgctgtg tcgcacaatt cgtggacgtg ggaaaattga tcccattctg gtgccggatc cggcgcgggt agccgagatg ctggcaccgg tattaaccgg taacgacctg attctcgttc agggggctgg taatattgga aaaattgccc gttctttagc tgaaatcaaa ctgaagccgc aaactccgga ggaagaacaa catgactaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNTQQLAKL RSIVPEMRRV RHIHFVGIGG AGMGGIAEVL ANEGYQISGS DLAPNPVTQQ LMNLGATIYF NHRPENVRDA SVVVVSSAIS ADNPEIVAAH EARIPVIRRA EMLAELMRFR HGIAIAGTHG KTTTTAMVSS IYAEAGLDPT FVNGGLVKAA GVHARLGHGR YLIAEADESD ASFLHLQPMV AIVTNIEADH MDTYQGDFEN LKQTFINFLH NLPFYGRAVM CVDDPVIREL LPRVGRQTTT YGFSEDADVR VEDYQQIGPQ GHFTLLRQDK EPMRVTLNAP GRHNALNAAA AVAVATEEGI DDEAILRALE SFQGTGRRFD FLGEFPLEPV NGKSGTAMLV DDYGHHPTEV DATIKAARAG WPDKNLVMLF QPHRFTRTRD LYDDFANVLT QVDTLLMLEV YPAGEAPIPG ADSRSLCRTI RGRGKIDPIL VPDPARVAEM LAPVLTGNDL ILVQGAGNIG KIARSLAEIK LKPQTPEEEQ HD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATACACAA CAATTGGCAA AACTGCGTTC CATCGTGCCC GAAATGCGTC GCGTTCGGCA CATACATTTT GTCGGCATTG GTGGTGCCGG TATGGGCGGT ATTGCCGAAG TTCTGGCCAA TGAAGGTTAT CAGATCAGTG GTTCCGATTT AGCGCCAAAT CCGGTCACGC AGCAGTTAAT GAATCTGGGT GCGACGATTT ATTTCAACCA TCGCCCGGAA AACGTACGTG ATGCCAGCGT GGTCGTTGTT TCCAGCGCGA TTTCTGCCGA TAACCCGGAA ATTGTCGCCG CTCATGAAGC GCGTATTCCG GTGATCCGTC GTGCCGAAAT GCTGGCTGAG TTAATGCGTT TTCGTCATGG CATCGCCATT GCCGGAACGC ACGGCAAAAC GACAACCACC GCGATGGTTT CCAGCATCTA CGCAGAAGCG GGGCTCGACC CAACCTTCGT TAACGGCGGG CTGGTAAAAG CGGCGGGGGT TCATGCGCGT TTGGGGCATG GTCGGTACCT GATTGCCGAA GCAGATGAGA GTGATGCATC GTTCCTGCAT CTGCAACCGA TGGTGGCGAT TGTCACCAAT ATCGAAGCCG ACCACATGGA TACCTACCAG GGCGACTTTG AGAATTTAAA ACAGACTTTT ATTAATTTTC TGCACAACCT GCCGTTTTAC GGTCGTGCGG TGATGTGTGT TGATGATCCG GTGATCCGCG AATTGTTACC GCGAGTGGGG CGTCAGACCA CGACTTACGG CTTCAGCGAA GATGCCGACG TGCGTGTAGA AGATTATCAG CAGATTGGCC CGCAGGGGCA CTTTACGCTG CTGCGCCAGG ACAAAGAGCC GATGCGCGTC ACCCTGAATG CGCCAGGTCG TCATAACGCG CTGAACGCCG CAGCTGCGGT TGCGGTTGCT ACGGAAGAGG GCATTGACGA CGAGGCTATT TTGCGGGCGC TTGAAAGCTT CCAGGGGACT GGTCGCCGTT TTGATTTCCT CGGTGAATTC CCGCTGGAGC CAGTGAATGG TAAAAGCGGT ACGGCAATGC TGGTCGATGA CTACGGCCAC CACCCGACGG AAGTGGACGC CACCATTAAA GCGGCGCGCG CAGGCTGGCC GGATAAAAAC CTGGTAATGC TGTTTCAGCC GCACCGTTTT ACCCGTACGC GCGACCTGTA TGATGATTTC GCCAATGTGC TGACGCAGGT TGATACCCTG TTGATGCTGG AAGTGTATCC GGCTGGCGAA GCGCCAATTC CGGGAGCGGA CAGCCGTTCG CTGTGTCGCA CAATTCGTGG ACGTGGGAAA ATTGATCCCA TTCTGGTGCC GGATCCGGCG CGGGTAGCCG AGATGCTGGC ACCGGTATTA ACCGGTAACG ACCTGATTCT CGTTCAGGGG GCTGGTAATA TTGGAAAAAT TGCCCGTTCT TTAGCTGAAA TCAAACTGAA GCCGCAAACT CCGGAGGAAG AACAACATGA CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA
Details for EscoA.00137.a.AE1.PW39151
PURIFICATION DATe: 9/22/2022
CONCENTRATION: 17.52mg/ml
OBSERVED MW: 54kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: Rosetta2 (DE3)
VIAL COUNT (approx.): 19
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MNTQQLAKLR SIVPEMRRVR HIHFVGIGGA GMGGIAEVLA NEGYQISGSD LAPNPVTQQL MNLGATIYFN HRPENVRDAS VVVVSSAISA DNPEIVAAHE ARIPVIRRAE MLAELMRFRH GIAIAGTHGK TTTTAMVSSI YAEAGLDPTF VNGGLVKAAG VHARLGHGRY LIAEADESDA SFLHLQPMVA IVTNIEADHM DTYQGDFENL KQTFINFLHN LPFYGRAVMC VDDPVIRELL PRVGRQTTTY GFSEDADVRV EDYQQIGPQG HFTLLRQDKE PMRVTLNAPG RHNALNAAAA VAVATEEGID DEAILRALES FQGTGRRFDF LGEFPLEPVN GKSGTAMLVD DYGHHPTEVD ATIKAARAGW PDKNLVMLFQ PHRFTRTRDL YDDFANVLTQ VDTLLMLEVY PAGEAPIPGA DSRSLCRTIR GRGKIDPILV PDPARVAEML APVLTGNDLI LVQGAGNIGK IARSLAEIKL KPQTPEEEQH DAGHHHHHH
Validated NT Sequence
atgaatacac aacaattggc aaaactgcgt tccatcgtgc ccgaaatgcg tcgcgttcgg cacatacatt ttgtcggcat tggtggtgcc ggtatgggcg gtattgccga agttctggcc aatgaaggtt atcagatcag tggttccgat ttagcgccaa atccggtcac gcagcagtta atgaatctgg gtgcgacgat ttatttcaac catcgcccgg aaaacgtacg tgatgccagc gtggtcgttg tttccagcgc gatttctgcc gataacccgg aaattgtcgc cgctcatgaa gcgcgtattc cggtgatccg tcgtgccgaa atgctggctg agttaatgcg ttttcgtcat ggcatcgcca ttgccggaac gcacggcaaa acgacaacca ccgcgatggt ttccagcatc tacgcagaag cggggctcga cccaaccttc gttaacggcg ggctggtaaa agcggcgggg gttcatgcgc gtttggggca tggtcggtac ctgattgccg aagcagatga gagtgatgca tcgttcctgc atctgcaacc gatggtggcg attgtcacca atatcgaagc cgaccacatg gatacctacc agggcgactt tgagaattta aaacagactt ttattaattt tctgcacaac ctgccgtttt acggtcgtgc ggtgatgtgt gttgatgatc cggtgatccg cgaattgtta ccgcgagtgg ggcgtcagac cacgacttac ggcttcagcg aagatgccga cgtgcgtgta gaagattatc agcagattgg cccgcagggg cactttacgc tgctgcgcca ggacaaagag ccgatgcgcg tcaccctgaa tgcgccaggt cgtcataacg cgctgaacgc cgcagctgcg gttgcggttg ctacggaaga gggcattgac gacgaggcta ttttgcgggc gcttgaaagc ttccagggga ctggtcgccg ttttgatttc ctcggtgaat tcccgctgga gccagtgaat ggtaaaagcg gtacggcaat gctggtcgat gactacggcc accacccgac ggaagtggac gccaccatta aagcggcgcg cgcaggctgg ccggataaaa acctggtaat gctgtttcag ccgcaccgtt ttacccgtac gcgcgacctg tatgatgatt tcgccaatgt gctgacgcag gttgataccc tgttgatgct ggaagtgtat ccggctggcg aagcgccaat tccgggagcg gacagccgtt cgctgtgtcg cacaattcgt ggacgtggga aaattgatcc cattctggtg ccggatccgg cgcgggtagc cgagatgctg gcaccggtat taaccggtaa cgacctgatt ctcgttcagg gggctggtaa tattggaaaa attgcccgtt ctttagctga aatcaaactg aagccgcaaa ctccggagga agaacaacat gacgctgggc accaccatca tcatcattaa
Expressed Protein Sequence
MNTQQLAKLR SIVPEMRRVR HIHFVGIGGA GMGGIAEVLA NEGYQISGSD LAPNPVTQQL MNLGATIYFN HRPENVRDAS VVVVSSAISA DNPEIVAAHE ARIPVIRRAE MLAELMRFRH GIAIAGTHGK TTTTAMVSSI YAEAGLDPTF VNGGLVKAAG VHARLGHGRY LIAEADESDA SFLHLQPMVA IVTNIEADHM DTYQGDFENL KQTFINFLHN LPFYGRAVMC VDDPVIRELL PRVGRQTTTY GFSEDADVRV EDYQQIGPQG HFTLLRQDKE PMRVTLNAPG RHNALNAAAA VAVATEEGID DEAILRALES FQGTGRRFDF LGEFPLEPVN GKSGTAMLVD DYGHHPTEVD ATIKAARAGW PDKNLVMLFQ PHRFTRTRDL YDDFANVLTQ VDTLLMLEVY PAGEAPIPGA DSRSLCRTIR GRGKIDPILV PDPARVAEML APVLTGNDLI LVQGAGNIGK IARSLAEIKL KPQTPEEEQH DGHHHHHH
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCAT GAATACACAA CAATTGGCAA AACTGCGTTC CATCGTGCCC GAAATGCGTC GCGTTCGGCA CATACATTTT GTCGGCATTG GTGGTGCCGG TATGGGCGGT ATTGCCGAAG TTCTGGCCAA TGAAGGTTAT CAGATCAGTG GTTCCGATTT AGCGCCAAAT CCGGTCACGC AGCAGTTAAT GAATCTGGGT GCGACGATTT ATTTCAACCA TCGCCCGGAA AACGTACGTG ATGCCAGCGT GGTCGTTGTT TCCAGCGCGA TTTCTGCCGA TAACCCGGAA ATTGTCGCCG CTCATGAAGC GCGTATTCCG GTGATCCGTC GTGCCGAAAT GCTGGCTGAG TTAATGCGTT TTCGTCATGG CATCGCCATT GCCGGAACGC ACGGCAAAAC GACAACCACC GCGATGGTTT CCAGCATCTA CGCAGAAGCG GGGCTCGACC CAACCTTCGT TAACGGCGGG CTGGTAAAAG CGGCGGGGGT TCATGCGCGT TTGGGGCATG GTCGGTACCT GATTGCCGAA GCAGATGAGA GTGATGCATC GTTCCTGCAT CTGCAACCGA TGGTGGCGAT TGTCACCAAT ATCGAAGCCG ACCACATGGA TACCTACCAG GGCGACTTTG AGAATTTAAA ACAGACTTTT ATTAATTTTC TGCACAACCT GCCGTTTTAC GGTCGTGCGG TGATGTGTGT TGATGATCCG GTGATCCGCG AATTGTTACC GCGAGTGGGG CGTCAGACCA CGACTTACGG CTTCAGCGAA GATGCCGACG TGCGTGTAGA AGATTATCAG CAGATTGGCC CGCAGGGGCA CTTTACGCTG CTGCGCCAGG ACAAAGAGCC GATGCGCGTC ACCCTGAATG CGCCAGGTCG TCATAACGCG CTGAACGCCG CAGCTGCGGT TGCGGTTGCT ACGGAAGAGG GCATTGACGA CGAGGCTATT TTGCGGGCGC TTGAAAGCTT CCAGGGGACT GGTCGCCGTT TTGATTTCCT CGGTGAATTC CCGCTGGAGC CAGTGAATGG TAAAAGCGGT ACGGCAATGC TGGTCGATGA CTACGGCCAC CACCCGACGG AAGTGGACGC CACCATTAAA GCGGCGCGCG CAGGCTGGCC GGATAAAAAC CTGGTAATGC TGTTTCAGCC GCACCGTTTT ACCCGTACGC GCGACCTGTA TGATGATTTC GCCAATGTGC TGACGCAGGT TGATACCCTG TTGATGCTGG AAGTGTATCC GGCTGGCGAA GCGCCAATTC CGGGAGCGGA CAGCCGTTCG CTGTGTCGCA CAATTCGTGG ACGTGGGAAA ATTGATCCCA TTCTGGTGCC GGATCCGGCG CGGGTAGCCG AGATGCTGGC ACCGGTATTA ACCGGTAACG ACCTGATTCT CGTTCAGGGG GCTGGTAATA TTGGAAAAAT TGCCCGTTCT TTAGCTGAAA TCAAACTGAA GCCGCAAACT CCGGAGGAAG AACAACATGA CGGGCACCAC CATCATCATC ATTAACGGAT CCGAATTCGA GCTCCGTCGA CAAGCTTGCG GCCGCACTCG AGCACCACCA CCACCACCAC TGAGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGAT
Details for EscoA.00137.a.HZ1.PW39198
PURIFICATION DATe: 3/10/2023
CONCENTRATION: 13.1mg/ml
OBSERVED MW: 68kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: Rosetta2 (DE3)
VIAL COUNT (approx.): 8
VIAL VOLUME: 55µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMN TQQLAKLRSI VPEMRRVRHI HFVGIGGAGM GGIAEVLANE GYQISGSDLA PNPVTQQLMN LGATIYFNHR PENVRDASVV VVSSAISADN PEIVAAHEAR IPVIRRAEML AELMRFRHGI AIAGTHGKTT TTAMVSSIYA EAGLDPTFVN GGLVKAAGVH ARLGHGRYLI AEADESDASF LHLQPMVAIV TNIEADHMDT YQGDFENLKQ TFINFLHNLP FYGRAVMCVD DPVIRELLPR VGRQTTTYGF SEDADVRVED YQQIGPQGHF TLLRQDKEPM RVTLNAPGRH NALNAAAAVA VATEEGIDDE AILRALESFQ GTGRRFDFLG EFPLEPVNGK SGTAMLVDDY GHHPTEVDAT IKAARAGWPD KNLVMLFQPH RFTRTRDLYD DFANVLTQVD TLLMLEVYPA GEAPIPGADS RSLCRTIRGR GKIDPILVPD PAQVAEMLAP VLTGNDLILV QGAGNIGKIA RSLAEIKLKP QTPEEEQHD
Validated NT Sequence
atggctcatc accatcacca tcatatgaag tcggactcag aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg ataacgatat tattgaggct cacagagaac agattggtgg taccctggaa gctcagaccc agggtcctgg ttcgatgaat acacaacaat tggcaaaact gcgttccatc gtgcccgaaa tgcgtcgcgt tcggcacata cattttgtcg gcattggtgg tgccggtatg ggcggtattg ccgaagttct ggccaatgaa ggttatcaga tcagtggttc cgatttagcg ccaaatccgg tcacgcagca gttaatgaat ctgggagcga cgatttattt caaccatcgc ccggaaaacg tacgtgatgc cagcgtggtc gttgtttcca gcgcgatttc tgccgataac ccggaaattg tcgcagctca tgaagcgcgt attccggtga tccgtcgtgc cgaaatgctg gctgagttaa tgcgttttcg tcatggcatc gccattgcag gaacgcacgg caaaacgaca accaccgcga tggtttccag catctacgca gaagcggggc tcgacccaac cttcgttaac ggcgggctgg taaaagcggc gggggttcat gcgcgtttgg ggcatggtcg gtacctgatt gccgaagcag atgagagtga tgcatcgttc ctgcatctgc aaccgatggt ggcgattgtc accaatatcg aagccgacca catggatacc taccagggcg actttgagaa tttaaaacag acttttatta attttctgca caacctgcct ttttacggtc gtgcggtgat gtgtgttgat gatccggtga tccgcgaatt gttaccgcga gtgggacgtc agaccacgac ttacggcttc agcgaagatg ccgacgtgcg tgtagaagat tatcagcaga ttggcccgca ggggcacttt acgctgctgc gccaggacaa agagccgatg cgcgtcaccc tgaatgcgcc aggtcgtcat aacgcgctga acgccgcagc tgcggttgcg gttgctacgg aagagggaat tgatgacgag gctattttac gtgcgctgga gagcttccag gggactgggc gccgttttga tttcctcggt gaattcccgc tggagccagt gaatggtaaa agcggtacgg cgatgttggt cgatgactac ggccaccacc cgacggaagt ggacgccact attaaagcgg cgcgtgcagg ctggccggat aaaaacctgg taatgctgtt tcagccgcac cgttttaccc gtacgcgcga cctgtatgat gatttcgcca atgtgctgac gcaggttgat accctgttga tgctggaagt gtatccggct ggcgaagcgc caattccggg agcggacagc cgttcgctgt gtcgcacaat tcgtggacgt gggaaaattg atcccattct ggtgccggac cctgcgcagg tagccgagat gctggcaccg gtattaaccg gtaacgacct gattctcgtt cagggggctg gtaatatcgg aaaaattgcc cgttctttag ctgaaatcaa actgaagccg caaactccgg aggaagaaca acatgactaa taa
Expressed Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMN TQQLAKLRSI VPEMRRVRHI HFVGIGGAGM GGIAEVLANE GYQISGSDLA PNPVTQQLMN LGATIYFNHR PENVRDASVV VVSSAISADN PEIVAAHEAR IPVIRRAEML AELMRFRHGI AIAGTHGKTT TTAMVSSIYA EAGLDPTFVN GGLVKAAGVH ARLGHGRYLI AEADESDASF LHLQPMVAIV TNIEADHMDT YQGDFENLKQ TFINFLHNLP FYGRAVMCVD DPVIRELLPR VGRQTTTYGF SEDADVRVED YQQIGPQGHF TLLRQDKEPM RVTLNAPGRH NALNAAAAVA VATEEGIDDE AILRALESFQ GTGRRFDFLG EFPLEPVNGK SGTAMLVDDY GHHPTEVDAT IKAARAGWPD KNLVMLFQPH RFTRTRDLYD DFANVLTQVD TLLMLEVYPA GEAPIPGADS RSLCRTIRGR GKIDPILVPD PARVAEMLAP VLTGNDLILV QGAGNIGKIA RSLAEIKLKP QTPEEEQHD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGAAGTCGGA CTCAGAAGTC AATCAAGAAG CTAAGCCAGA GGTCAAGCCA GAAGTCAAGC CTGAGACTCA CATCAATTTA AAGGTGTCCG ATGGATCTTC AGAGATCTTC TTCAAGATCA AAAAGACCAC TCCTTTAAGA AGGCTGATGG AAGCGTTCGC TAAAAGACAG GGTAAGGAAA TGGACTCCTT AAGATTCTTG TACGACGGTA TTAGAATTCA AGCTGATCAG ACCCCTGAAG ATTTGGACAT GGAGGATAAC GATATTATTG AGGCTCACAG AGAACAGATT GGTGGTACCC TGGAAGCTCA GACCCAGGGT CCTGGTTCGA TGAATACACA ACAATTGGCA AAACTGCGTT CCATCGTGCC CGAAATGCGT CGCGTTCGGC ACATACATTT TGTCGGCATT GGTGGTGCCG GTATGGGCGG TATTGCCGAA GTTCTGGCCA ATGAAGGTTA TCAGATCAGT GGTTCCGATT TAGCGCCAAA TCCGGTCACG CAGCAGTTAA TGAATCTGGG TGCGACGATT TATTTCAACC ATCGCCCGGA AAACGTACGT GATGCCAGCG TGGTCGTTGT TTCCAGCGCG ATTTCTGCCG ATAACCCGGA AATTGTCGCC GCTCATGAAG CGCGTATTCC GGTGATCCGT CGTGCCGAAA TGCTGGCTGA GTTAATGCGT TTTCGTCATG GCATCGCCAT TGCCGGAACG CACGGCAAAA CGACAACCAC CGCGATGGTT TCCAGCATCT ACGCAGAAGC GGGGCTCGAC CCAACCTTCG TTAACGGCGG GCTGGTAAAA GCGGCGGGGG TTCATGCGCG TTTGGGGCAT GGTCGGTACC TGATTGCCGA AGCAGATGAG AGTGATGCAT CGTTCCTGCA TCTGCAACCG ATGGTGGCGA TTGTCACCAA TATCGAAGCC GACCACATGG ATACCTACCA GGGCGACTTT GAGAATTTAA AACAGACTTT TATTAATTTT CTGCACAACC TGCCGTTTTA CGGTCGTGCG GTGATGTGTG TTGATGATCC GGTGATCCGC GAATTGTTAC CGCGAGTGGG GCGTCAGACC ACGACTTACG GCTTCAGCGA AGATGCCGAC GTGCGTGTAG AAGATTATCA GCAGATTGGC CCGCAGGGGC ACTTTACGCT GCTGCGCCAG GACAAAGAGC CGATGCGCGT CACCCTGAAT GCGCCAGGTC GTCATAACGC GCTGAACGCC GCAGCTGCGG TTGCGGTTGC TACGGAAGAG GGCATTGACG ACGAGGCTAT TTTGCGGGCG CTTGAAAGCT TCCAGGGGAC TGGTCGCCGT TTTGATTTCC TCGGTGAATT CCCGCTGGAG CCAGTGAATG GTAAAAGCGG TACGGCAATG CTGGTCGATG ACTACGGCCA CCACCCGACG GAAGTGGACG CCACCATTAA AGCGGCGCGC GCAGGCTGGC CGGATAAAAA CCTGGTAATG CTGTTTCAGC CGCACCGTTT TACCCGTACG CGCGACCTGT ATGATGATTT CGCCAATGTG CTGACGCAGG TTGATACCCT GTTGATGCTG GAAGTGTATC CGGCTGGCGA AGCGCCAATT CCGGGAGCGG ACAGCCGTTC GCTGTGTCGC ACAATTCGTG GACGTGGGAA AATTGATCCC ATTCTGGTGC CGGATCCGGC GCGGGTAGCC GAGATGCTGG CACCGGTATT AACCGGTAAC GACCTGATTC TCGTTCAGGG GGCTGGTAAT ATTGGAAAAA TTGCCCGTTC TTTAGCTGAA ATCAAACTGA AGCCGCAAAC TCCGGAGGAA GAACAACATG ACTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA