CENTER ID: | EscoA.00137.a |
ORGANISM: | Escherichia coli K12 |
ASSOCIATED DISEASE: | Food poisoning |
CURRENT STATUS: | purified |
COMMUNITY REQUEST: | True |
NIH RISK GROUP: | 1 |
SELECT AGENT: | False |
NIH PRIORITY pathogens category: |
IIIB |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
EscoA.00137.a.A1.GE44779 | full length | 1 | 491 | ||
EscoA.00137.a.AE1.GE44780 | full length | 1 | 491 | ||
EscoA.00137.a.HZ1.GE44781 | full length | 1 | 491 |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
EscoA.00137.a.A1.PW39150 | full length | 1 | 491 | ||
EscoA.00137.a.AE1.PW39151 | full length | 1 | 491 | ||
EscoA.00137.a.HZ1.PW39198 | full length | 1 | 491 |
RESOURCE | REFERENCE ID |
---|---|
BV-BRC: | fig|511145.12.peg.95 |
UniProt: | P17952 |
HARVESTED ON: | 8/12/2022 |
SEQUENCED ON: | 7/28/2022 |
EXPECTED MW: | 56kDa |
OBSERVED MW: | 56kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | High Expression |
SOLUBLE EXPRESSION LEVEL | High Expression |
EXPRESSION HOST: | Rosetta2 (DE3) |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |
HARVESTED ON: | 8/12/2022 |
SEQUENCED ON: | 7/28/2022 |
EXPECTED MW: | 54kDa |
OBSERVED MW: | 54kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | High Expression |
SOLUBLE EXPRESSION LEVEL | High Expression |
EXPRESSION HOST: | Rosetta2 (DE3) |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |
HARVESTED ON: | 8/12/2022 |
SEQUENCED ON: | 7/28/2022 |
EXPECTED MW: | 67kDa |
OBSERVED MW: | 67kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | High Expression |
SOLUBLE EXPRESSION LEVEL | High Expression |
EXPRESSION HOST: | Rosetta2 (DE3) |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 99 |
PERCENT COVERAGE: | 100 |
PURIFICATION DATe: | 9/21/2022 |
CONCENTRATION: | 11.3mg/ml |
OBSERVED MW: | 56kDa |
EXPRESSION LEVEL: | High Expression |
PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
EXPRESSION HOST: | Rosetta2 (DE3) |
VIAL COUNT (approx.): | 5 |
VIAL VOLUME: | 55µl |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |
PURIFICATION DATe: | 9/22/2022 |
CONCENTRATION: | 17.52mg/ml |
OBSERVED MW: | 54kDa |
EXPRESSION LEVEL: | High Expression |
PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
EXPRESSION HOST: | Rosetta2 (DE3) |
VIAL COUNT (approx.): | 19 |
VIAL VOLUME: | 110µl |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |
PURIFICATION DATe: | 3/10/2023 |
CONCENTRATION: | 13.1mg/ml |
OBSERVED MW: | 68kDa |
EXPRESSION LEVEL: | Moderate Expression |
PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
EXPRESSION HOST: | Rosetta2 (DE3) |
VIAL COUNT (approx.): | 8 |
VIAL VOLUME: | 55µl |
PERCENT IDENTITY: | 99 |
PERCENT COVERAGE: | 100 |