SSGCID: Target Page

EnhiA.20308.a

Ubiquitin carboxyl-terminal hydrolase, putative

CENTER ID:	EnhiA.20308.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.20308.a.B1.GE43143	full length		1	337

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI7A_003850
UniProt:	N9UVY3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSQSVKPKSL NLKKAYPLFG IKNFRSTCFA NSVFQVLCSL PPFVEYLKKV NPTDDMKYTK AIKDMFNLFL QCNKVGIQKE IIHPPQSMKE IIMKLQTESG QHQMDATEFY TTLINCLHTE CKNKISEGEE AQQDDDEGWE EVGKKFKKVQ LRSEKTDGTS VISDYFRGSM RSLTITIDEM KKKHNQAVLQ EFYVLSLPIQ SKQNCVSSVS QAINLAFSKR KIDNTHFQQL FIEEAPKVLV LQLNRFIYDR VKLDVVKLVD HINYTESLQL KTNKGNVNYR LISIIEHRGN QIRRGHYVSY VRRGEKWYFC NDENIQLIDQ KDIYDKMAYV LIYSSLN

NT Sequence

atgtcacaat ctgtaaaacc taaatcgttg aatttaaaga aagcataccc attgtttggc attaaaaatt ttaggtcaac atgttttgct aatagtgtat tccaggtatt atgttcttta ccaccttttg ttgaatattt aaaaaaagta aatcctacag atgatatgaa atatacaaaa gcaataaaag atatgtttaa cttatttctt caatgtaata aagttggaat tcaaaaagaa attattcatc cacctcaatc aatgaaggag attattatga aattacaaac agaaagtggt caacatcaaa tggatgcaac agaattttat actactttaa ttaactgttt acatactgaa tgtaaaaata aaatatctga aggagaagaa gcacaacaag atgatgatga gggatgggaa gaagttggaa aaaaattcaa aaaagttcaa ttaagaagtg aaaaaactga tggaacaagt gtaatttctg attattttag aggaagtatg aggtcattga ctataactat tgatgaaatg aaaaaaaaac acaaccaagc tgttttacaa gagttttatg ttttatcatt acccattcag tctaaacaaa attgcgttag ttctgttagt caagctatta atttagcttt ctcaaaaaga aaaattgata acacacattt tcaacaattg tttattgaag aagcaccaaa agtattagta ttacaattaa accgttttat ttatgatcgt gtgaaattag atgttgttaa attagttgat catattaatt atactgaatc tcttcaatta aaaacaaata aaggaaatgt aaattataga cttataagta ttattgaaca tcgtgggaat caaataagaa gaggacatta tgtaagttat gttagaagag gggagaaatg gtatttttgt aatgatgaaa atattcaact cattgaccaa aaagatattt atgataagat ggcatatgtt ttaatttatt cttctttaaa ttaa

Details for EnhiA.20308.a.B1.GE43143

HARVESTED ON:	5/17/2019
SEQUENCED ON:	5/24/2019
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMS QSVKPKSLNL KKAYPLFGIK NFRSTCFANS VFQVLCSLPP FVEYLKKVNP TDDMKYTKAI KDMFNLFLQC NKVGIQKEII HPPQSMKEII MKLQTESGQH QMDATEFYTT LINCLHTECK NKISEGEEAQ QDDDEGWEEV GKKFKKVQLR SEKTDGTSVI SDYFRGSMRS LTITIDEMKK KHNQAVLQEF YVLSLPIQSK QNCVSSVSQA INLAFSKRKI DNTHFQQLFI EEAPKVLVLQ LNRFIYDRVK LDVVKLVDHI NYTESLQLKT NKGNVNYRLI SIIEHRGNQI RRGHYVSYVR RGEKWYFCND ENIQLIDQKD IYDKM

Validated NT Sequence

tgccatctta tcataaatat ctttttggtc aatgagttga atattttcat cattacaaaa ataccatttc tcccctcttc taacataact tacataatgt cctcttctta tttgattccc acgatgttca ataatactta taagtctata atttacattt cctttatttg tttttaattg aagagattca gtataattaa tatgatcaac taatttaaca acatctaatt tcacacgatc ataaataaaa cggtttaatt gtaatactaa tacttttggt gcttcttcaa taaacaattg ttgaaaatgt gtgttatcaa tttttctttt tgagaaagct aaattaatag cttgactaac agaactaacg caattttgtt tagactgaat gggtaatgat aaaacataaa actcttgtaa aacagcttgg ttgtgttttt ttttcatttc atcaatagtt atagtcaatg acctcatact tcctctaaaa taatcagaaa ttacacttgt tccatcagtt ttttcacttc ttaattgaac ttttttgaat ttttttccaa cttcttccca tccctcatca tcatcttgtt gtgcttcttc tccttcagat attttatttt tacattcagt atgtaaacag ttaattaaag tagtataaaa ttctgttgca tccatttgat gttgaccact ttctgtttgt aatttcataa taatctcctt cattgattga ggtggatgaa taatttcttt ttgaattcca actttattac attgaagaaa taagttaaac atatctttta ttgcttttgt atatttcata tcatctgtag gatttacttt ttttaaatat tcaacaaaag gtggtaaaga acataatacc tggaatacac tattagcaaa acatgttgac ctaaaatttt taatgccaaa caatgggtat gctttcttta aattcaacga tttaggtttt acagattgtg acatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcac aatctgtaaa acctaaatcg ttgaatttaa agaaagcata cccattgttt ggcattaaaa attttaggtc aacatgtttt gctaatagtg tattccaggt attatgttct ttaccacctt ttgttgaata tttaaaaaaa gtaaatccta cagatgatat gaaatataca aaagcaataa aagatatgtt taacttattt cttcaatgta ataaagttgg aattcaaaaa gaaattattc atccacctca atcaatgaag gagattatta tgaaattaca aacagaaagt ggtcaacatc aaatggatgc aacagaattt tatactactt taattaactg tttacatact gaatgtaaaa ataaaatatc tgaaggagaa gaagcacaac aagatgatga tgagggatgg gaagaagttg gaaaaaaatt caaaaaagtt caattaagaa gtgaaaaaac tgatggaaca agtgtaattt ctgattattt tagaggaagt atgaggtcat tgactataac tattgatgaa atgaaaaaaa aacacaacca agctgtttta caagagtttt atgttttatc attacccatt cagtctaaac aaaattgcgt tagttctgtt agtcaagcta ttaatttagc tttctcaaaa agaaaaattg ataacacaca ttttcaacaa ttgtttattg aagaagcacc aaaagtatta gtattacaat taaaccgttt tatttatgat cgtgtgaaat tagatgttgt taaattagtt gatcatatta attatactga atctcttcaa ttaaaaacaa ataaaggaaa tgtaaattat agacttataa gtattattga acatcgtggg aatcaaataa gaagaggaca ttatgtaagt tatgttagaa gaggggagaa atggtatttt tgtaatgatg aaaatattca actcattgac caaaaagata tttatgataa gatggcatat gttttaattt attcttcttt aaattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat