SSGCID: Target Page

EnhiA.18525.a

metallosurface protease 1 (EhMSP-1)

CENTER ID:	EnhiA.18525.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.18525.a.B2.GE36446	domain 1		42	391
EnhiA.18525.a.B3.GE36447	domain 2		379	597

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_200230
RefSeq:	XP_655394.1
UniProt:	C4M7M4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLIVLLLISF TFAECIFSQI QQKTESPLYH PKRKLASDDS EWVPLNVKFD TNALNYGSGY VSTPPVCFVV SGTCTQDNLL TQEKKAYIIR IIDEVQRLIK KYFRVHKGTL ATLKSSIKDK DRCGEISSIK DSSIADDIGM VMYVTAHPIE SQTVLAYAAS CGSAADASST NPNNQKRNIF GYTNINPANL DVSEGKFRIN AHTVLHETMH AMGFVSPTGM MSISKGRGTE TVPVVTSEKV LKVAREHFGD NSISYVEFED GGGSGTAGAH WEKRVLYNEI MTGTASSYSV ISNFTLAYFE DLGTYSVNYS AAEPLTWGKG MKKDFFKCSN WPTQAPYYGE TQARGCTPDR GAIGICDTSV RKDLPKIYQN YEDPTKGGMI ELMDYCIHTT LVSGGQCYEK SVLSTENIAS LSFLDRGSSY GKDSRCFSSS LMKYSIPISD FSCYRVKCVD RGYRVNVNGN WILCPSGDSI SVTGYGGVIT CVNQSELCNG EVEEWPDIWR TDPVKGKAGS IVTLIGDYFS HMKKVYVGET EQTQFSIDNS NQVRVKIQFN DPFVNLIQLL SDGYVTVDIK IGDGNDINAV YQNFKLQVEL VEVVQNVGQW LYKNLFFTVG IIIFLIFLVL LFGFIITKRI IYRRAKQVAR NLV

NT Sequence

atgttgattg tattactact catctcattt acattcgctg aatgtatctt ttctcaaata caacaaaaaa cagaaagtcc attgtatcat ccaaaaagaa aattagcaag tgatgactct gaatgggttc cattaaatgt taagtttgat actaatgcat taaattatgg tagtggatat gtaagtactc cacctgtttg ttttgttgta tcaggaacct gtacacaaga taatttgtta acacaagaga aaaaggcata tattataaga attatagacg aagtacaacg tttaattaag aaatatttta gagtacataa aggaacgtta gccacattaa aatcaagtat aaaagataaa gatagatgtg gagaaatttc tagtataaaa gatagctcaa ttgctgatga tattggaatg gtaatgtatg ttacggcaca tcctattgaa tcacaaactg tattagctta tgctgcatca tgtggaagtg ctgctgatgc aagctctact aatccaaata atcagaaacg aaatattttt ggttatacaa atattaatcc agcaaattta gatgttagtg aaggaaaatt cagaatcaat gcccatactg tccttcatga aacaatgcat gcaatgggat ttgtatcacc tactggtatg atgtctattt ctaaaggaag aggaacagag acagtacctg ttgttaccag tgaaaaagtt cttaaagttg ctcgagaaca ttttggtgat aattcaattt catatgtaga gtttgaggat ggtggaggaa gtggaactgc aggagctcat tgggaaaaaa gagttcttta taatgaaatt atgacaggaa cagcatcatc ttattcagtc atttctaatt tcacattggc atattttgaa gacctaggaa cttattcagt taattatagt gctgctgaac cattaacatg gggtaaaggt atgaaaaaag attttttcaa atgttctaat tggccaacac aagcgccata ttatggtgag actcaagcaa ggggatgtac tcctgataga ggagccattg gaatttgtga tacttctgtt cgtaaagatt tgcctaaaat ttatcaaaat tatgaagacc caactaaagg agggatgatt gaattaatgg attattgtat tcacacaaca ttagtttcag gaggtcaatg ttatgaaaag agtgtattat caacagaaaa tattgcctca ttatcattcc ttgatagagg aagttcatat ggaaaagatt caagatgttt ttcatctagt ttaatgaaat attctattcc aattagtgac tttagttgtt atagagtaaa atgtgtagac cgaggatata gagttaatgt taatggaaat tggattcttt gtccatcagg agatagtatt agtgttactg gatatggtgg agtaattaca tgtgtgaatc aaagtgaatt atgtaatggc gaagtagaag aatggccaga tatatggagg acagacccag taaaaggaaa agcagggtct atagtaacat taattggtga ttatttcagt cacatgaaaa aagtatatgt tggagaaaca gaacaaacac aattttctat tgataattcc aatcaagtca gagtaaaaat tcagtttaat gatccttttg ttaatcttat tcaacttctt tctgatggtt atgttacagt agatattaaa attggagatg gaaatgatat aaatgcagta tatcaaaatt ttaaattaca agttgaatta gttgaagtag tacaaaatgt tggtcaatgg ttatataaaa atctcttctt tacagttggt attattatct tccttatctt cttagtgtta ttatttggtt ttatcattac aaaaagaatc atttatagaa gagcaaaaca agttgcaaga aatcttgttt aa

Details for EnhiA.18525.a.B2.GE36446

HARVESTED ON:	11/21/2012
SEQUENCED ON:	11/27/2012
EXPECTED MW:	39kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	87
PERCENT COVERAGE:	91

Validated AA Sequence

MAHHHHHHMW VPLNVKFDTN ALNYGSGYVS TPPVCFVVSG TCTQDNLLTQ EKKAYIIRII DEVQRLIKKY FRVHKGTLAT LKSSIKDKDR CGEISSIKDS SIADDIGMVM YVTAHPIESQ TVLAYAASCG SAADASSTNP NNQKRNIFGY TNINPANLDV SEGKFRINAH TVLHETMHAM GFVSPTGMMS ISKGRGTETV PVVTSEKVLK VAREHFGDNS ISYVEFEDGG GSGTAGAHWE KRVLYNEIMT GTASSYSVIS NFXLXYFEXL GTYSVNYSAA EXLXWGKGMK XIFSNVLXXT SXXXXEXXXG XYXXXXXXXT XVXXIANXX

Validated NT Sequence

cnnnnnnnnn ttgtnnnntt taagaaggag anataccatg gctcaccacc accaccacca tatgtgggtt ccattaaatg ttaagtttga tactaatgca ttaaattatg gtagtggata tgtaagtact ccacctgttt gttttgttgt atcaggaacc tgtacacaag ataatttgtt aacacaagag aaaaaggcat atattataag aattatagac gaagtacaac gtttaattaa gaaatatttt agagtacata aaggaacgtt agccacatta aaatcaagta taaaagataa agatagatgt ggagaaattt ctagtataaa agatagctca attgctgatg atattggaat ggtaatgtat gttacggcac atcctattga atcacaaact gtattagctt atgctgcatc atgtggaagt gctgctgatg caagctctac taatccaaat aatcagaaac gaaatatttt tggttataca aatattaatc cagcaaattt agatgttagt gaaggaaaat tcagaatcaa tgcccatact gtccttcatg aaacaatgca tgcaatggga tttgtatcac ctactggtat gatgtctatt tctaaaggaa gaggaacaga gacagtacct gttgttacca gtgaaaaagt tcttaaagtt gctcgagaac attttggtga taattcaatt tcatatgtag agtttgagga tggtggagga agtggaactg cnggagctca ttgggaaaaa agagttcttt ataatgaaat tatgacagga acngcatcat cttattcagt catttctaat ttcncattgg nntattttga aganctagga acttattcag ttaattatag tgctgctgaa nnnttancat ggggtaaagg natgaaaaan attttttcaa atgtnctann tgncacnagc gncntannng nngagnctca ncnngggnng tacnctgnna ncntnnnnnn nnanactnnn gtnnnaanna ttgcnaattn tnaatgannc cnnnnnnngg nngntgatnn ngntnnnnnn nnnnnnnnnn annannnaaa aa

Expected Protein Sequence

MAHHHHHHMW VPLNVKFDTN ALNYGSGYVS TPPVCFVVSG TCTQDNLLTQ EKKAYIIRII DEVQRLIKKY FRVHKGTLAT LKSSIKDKDR CGEISSIKDS SIADDIGMVM YVTAHPIESQ TVLAYAASCG SAADASSTNP NNQKRNIFGY TNINPANLDV SEGKFRINAH TVLHETMHAM GFVSPTGMMS ISKGRGTETV PVVTSEKVLK VAREHFGDNS ISYVEFEDGG GSGTAGAHWE KRVLYNEIMT GTASSYSVIS NFTLAYFEDL GTYSVNYSAA EPLTWGKGMK KDFFKCSNWP TQAPYYGETQ ARGCTPDRGA IGICDTSVRK DLPKIYQNYE DPTKGGMIEL MDYCIHTTL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtggg ttccattaaa tgttaagttt gatactaatg cattaaatta tggtagtgga tatgtaagta ctccacctgt ttgttttgtt gtatcaggaa cctgtacaca agataatttg ttaacacaag agaaaaaggc atatattata agaattatag acgaagtaca acgtttaatt aagaaatatt ttagagtaca taaaggaacg ttagccacat taaaatcaag tataaaagat aaagatagat gtggagaaat ttctagtata aaagatagct caattgctga tgatattgga atggtaatgt atgttacggc acatcctatt gaatcacaaa ctgtattagc ttatgctgca tcatgtggaa gtgctgctga tgcaagctct actaatccaa ataatcagaa acgaaatatt tttggttata caaatattaa tccagcaaat ttagatgtta gtgaaggaaa attcagaatc aatgcccata ctgtccttca tgaaacaatg catgcaatgg gatttgtatc acctactggt atgatgtcta tttctaaagg aagaggaaca gagacagtac ctgttgttac cagtgaaaaa gttcttaaag ttgctcgaga acattttggt gataattcaa tttcatatgt agagtttgag gatggtggag gaagtggaac tgcaggagct cattgggaaa aaagagttct ttataatgaa attatgacag gaacagcatc atcttattca gtcatttcta atttcacatt ggcatatttt gaagacctag gaacttattc agttaattat agtgctgctg aaccattaac atggggtaaa ggtatgaaaa aagatttttt caaatgttct aattggccaa cacaagcgcc atattatggt gagactcaag caaggggatg tactcctgat agaggagcca ttggaatttg tgatacttct gttcgtaaag atttgcctaa aatttatcaa aattatgaag acccaactaa aggagggatg attgaattaa tggattattg tattcacaca acattatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for EnhiA.18525.a.B3.GE36447

HARVESTED ON:	11/21/2012
SEQUENCED ON:	11/27/2012
EXPECTED MW:	25kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMI ELMDYCIHTT LVSGGQCYEK SVLSTENIAS LSFLDRGSSY GKDSRCFSSS LMKYSIPISD FSCYRVKCVD RGYRVNVNGN WILCPSGDSI SVTGYGGVIT CVNQSELCNG EVEEWPDIWR TDPVKGKAGS IVTLIGDYFS HMKKVYVGET EQTQFSIDNS NQVRVKIQFN DPFVNLIQLL SDGYVTVDIK IGDGNDINAV YQNFKLQVEL VEVVQNV

Validated NT Sequence

antttnnnnn nnttaagaag gagatatacc atggctcacc accaccacca ccatatgatt gaattaatgg attattgtat tcacacaaca ttagtttcag gaggtcaatg ttatgaaaag agtgtattat caacagaaaa tattgcctca ttatcattcc ttgatagagg aagttcatat ggaaaagatt caagatgttt ttcatctagt ttaatgaaat attctattcc aattagtgac tttagttgtt atagagtaaa atgtgtagac cgaggatata gagttaatgt taatggaaat tggattcttt gtccatcagg agatagtatt agtgttactg gatatggtgg agtaattaca tgtgtgaatc aaagtgaatt atgtaatggc gaagtagaag aatggccaga tatatggagg acagacccag taaaaggaaa agcagggtct atagtaacat taattggtga ttatttcagt cacatgaaaa aagtatatgt tggagaaaca gaacaaacac aattttctat tgataattcc aatcaagtca gagtaaaaat tcagtttaat gatccttttg ttaatcttat tcaacttctt tctgatggtt atgttacagt agatattaaa attggagatg gaaatgatat aaatgcagta tatcaaaatt ttaaattaca agttgaatta gttgaagtag tacaaaatgt ttaagtgagt aagataggat ccggntnnnn nncccccnnn nnnnnnnnnn nnngggnnnn nnnnnnnnnn cctncannnc nttganntaa nnnnntnntg cnnnnnnnnn nnnnnnnnac nct

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattg aattaatgga ttattgtatt cacacaacat tagtttcagg aggtcaatgt tatgaaaaga gtgtattatc aacagaaaat attgcctcat tatcattcct tgatagagga agttcatatg gaaaagattc aagatgtttt tcatctagtt taatgaaata ttctattcca attagtgact ttagttgtta tagagtaaaa tgtgtagacc gaggatatag agttaatgtt aatggaaatt ggattctttg tccatcagga gatagtatta gtgttactgg atatggtgga gtaattacat gtgtgaatca aagtgaatta tgtaatggcg aagtagaaga atggccagat atatggagga cagacccagt aaaaggaaaa gcagggtcta tagtaacatt aattggtgat tatttcagtc acatgaaaaa agtatatgtt ggagaaacag aacaaacaca attttctatt gataattcca atcaagtcag agtaaaaatt cagtttaatg atccttttgt taatcttatt caacttcttt ctgatggtta tgttacagta gatattaaaa ttggagatgg aaatgatata aatgcagtat atcaaaattt taaattacaa gttgaattag ttgaagtagt acaaaatgtt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat