EnhiA.18501.a

Cyclin, putative

CENTER ID: EnhiA.18501.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.18501.a.B1.GE35804 full length 1 400
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.18501.a.B1.PW36444 full length 1 400

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_166420
RefSeq: XP_652267.2
UniProt: C4M7E8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVTRDTENKS EIAIKPLINQ IGFRQHHVIQ PPTAKINLNH SSQKSKDSQE QKQFVEKDDE SIEKELVKLE IKPSNAFELI QLTNDEIISK QPEIYLSSNE NKDLNEFLTK NTNDVILESQ TQFDEQIEDI DQYEKDHFLM RYEPKNIKEI SDNLFKSEKD LQIAPDLLQK QVHISAVTRN VLLDWLFLVS QDEFATTIET YLLTVATFDD FISRKTTIPK FVQLVGVACL HLCVKFQEIT YPHVDEFIII SKGAYTKNQL LEMERVICKT IDFRFLRPLS IDFLRRFDKA ANADEIKHTL GKYLILIATL DQKMIQCLPS QIAAAAVYFQ RRINNTVPYW DKTLIKYSTY TEKDILPIAQ LMRTHYLFQQ SRTNQYKEMF KMFSSSVNHT VSQIPFIPEF
NT Sequence
atggttacaa gagatactga aaataagagc gaaattgcca ttaaaccatt aattaaccaa attggatttc gacaacatca tgtcatccaa ccaccaacag ccaagattaa tcttaatcat tcatcccaaa aaagtaaaga cagtcaagaa caaaaacagt ttgttgaaaa agatgatgaa tcaattgaaa aagaattagt taaattggaa attaaaccaa gtaatgcgtt tgaattaatc caattaacaa atgatgaaat aatatctaaa caacctgaaa tttatctatc ttcaaatgaa aataaagatt taaatgagtt tctaacaaaa aataccaatg atgttatttt agaaagtcaa actcaatttg atgaacaaat agaagacatt gatcaatatg aaaaagatca tttcttaatg aggtatgaac caaaaaatat taaagaaata tctgataact tatttaaaag tgaaaaggat ttacaaattg caccagattt acttcaaaaa caagttcata ttagtgcagt tacacgtaat gttttacttg actggttatt tcttgtttca caagatgaat ttgcaacaac aatagaaaca tatttactca ctgttgcaac atttgatgat tttatttctc gcaaaacaac tatccctaag tttgttcaat tagttggtgt tgcttgtctt catttatgtg ttaaatttca agaaatcaca tacccacatg ttgatgaatt tattattatt agtaaaggtg catatacaaa aaaccaatta ttagagatgg aaagagtgat ttgtaaaaca attgatttca gatttcttcg tccattatca attgattttt tgagaagatt tgataaggca gcaaatgcag atgaaattaa acatactctt ggaaagtatt taattttaat tgctacatta gaccaaaaaa tgattcaatg tcttccatct caaattgcag cagctgctgt ttattttcag agaagaatca ataatactgt tccttattgg gataaaacac ttattaaata ctcaacatat actgaaaaag atattcttcc tattgcccaa ttaatgagaa cgcattattt attccaacaa tcaagaacaa atcaatataa agaaatgttt aaaatgtttt caagtagtgt taatcacaca gtctcacaaa ttcctttcat cccagaattt taa
Details for EnhiA.18501.a.B1.GE35804
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/12/2013
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHMV TRDTENKSEI AIKPLINQIG FRQHHVIQPP TAKINLNHSS QKSKDSQEQS NLVNTLEDKL PNLEQFVEKD DESIEKELVK LEIKPSNAFE LIQLTNDEII SKQPEIYLSS NENKDLNEFL TKNTNDVILE SQTQFDEQIE DIDQYEKDHF LMRYEPKNIK EISDNLFKSE KDLQIAPDLL QKQVHISAVT RNVLLDWLFL VSQDEFATTI ETYLLTVATF DDFISRKTTI PKSEVQLVGV ACLHLCVKFQ EITYPHVDEF IIISKGAYTK NQLLEMERVI CKTIDFRFLR PLSIDFLRRF DKAANADEIK HTLGKYLILI ATLDQKMIQC LPSQIAAAAV YFQRRINNTV PYWDKTLIKY STYTEKDILP IAQLMRTHYL FQQSRTNQYK EMFKMFSSSV NHTV
Validated NT Sequence
atggctcacc accaccacca ccatatggtt acaagagata ctgaaaataa gagcgaaatt gccattaaac cattaattaa ccaaattgga tttcgacaac atcatgtcat ccaaccacca acagccaaga ttaatcttaa tcattcatcc caaaaaagta aagacagtca agaacaaagt aatttagtaa ataccctgga agacaaatta cctaatttag aacagtttgt tgaaaaagat gatgaatcaa ttgaaaaaga attagttaaa ttggaaatta aaccaagtaa tgcgtttgaa ttaatccaat taacaaatga tgaaataata tctaaacaac ctgaaattta tctatcttca aatgaaaata aagatttaaa tgagtttcta acaaaaaata ccaatgatgt tattttagaa agtcaaactc aatttgatga acaaatagaa gacattgatc aatatgaaaa agatcatttc ttaatgaggt atgaaccaaa aaatattaaa gaaatatctg ataacttatt taaaagtgaa aaggatttac aaattgcacc agatttactt caaaaacaag ttcatattag tgcagttaca cgtaatgttt tacttgactg gttatttctt gtttcacaag atgaatttgc aacaacaata gaaacatatt tactcactgt tgcaacattt gatgatttta tttctcgcaa aacaactatc cctaaatcag aggttcaatt agttggtgtt gcttgtcttc atttatgtgt taaatttcaa gaaatcacat acccacatgt tgatgaattt attattatta gtaaaggtgc atatacaaaa aaccaattat tagagatgga aagagtgatt tgtaaaacaa ttgatttcag atttcttcgt ccattatcaa ttgatttttt gagaagattt gataaggcag caaatgcaga tgaaattaaa catactcttg gaaagtattt aattttaatt gctacattag accaaaaaat gattcaatgt cttccatctc aaattgcagc agctgctgtt tattttcaga gaagaatcaa taatactgtt ccttattggg ataaaacact tattaaatac tcaacatata ctgaaaaaga tattcttcct attgcccaat taatgagaac gcattattta ttccaacaat caagaacaaa tcaatataaa gaaatgttta aaatgttttc aagtagtgtt aatcacacag tctc
Expected Protein Sequence
MAHHHHHHMV TRDTENKSEI AIKPLINQIG FRQHHVIQPP TAKINLNHSS QKSKDSQEQK QFVEKDDESI EKELVKLEIK PSNAFELIQL TNDEIISKQP EIYLSSNENK DLNEFLTKNT NDVILESQTQ FDEQIEDIDQ YEKDHFLMRY EPKNIKEISD NLFKSEKDLQ IAPDLLQKQV HISAVTRNVL LDWLFLVSQD EFATTIETYL LTVATFDDFI SRKTTIPKFV QLVGVACLHL CVKFQEITYP HVDEFIIISK GAYTKNQLLE MERVICKTID FRFLRPLSID FLRRFDKAAN ADEIKHTLGK YLILIATLDQ KMIQCLPSQI AAAAVYFQRR INNTVPYWDK TLIKYSTYTE KDILPIAQLM RTHYLFQQSR TNQYKEMFKM FSSSVNHTVS QIPFIPEF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtta caagagatac tgaaaataag agcgaaattg ccattaaacc attaattaac caaattggat ttcgacaaca tcatgtcatc caaccaccaa cagccaagat taatcttaat cattcatccc aaaaaagtaa agacagtcaa gaacaaaaac agtttgttga aaaagatgat gaatcaattg aaaaagaatt agttaaattg gaaattaaac caagtaatgc gtttgaatta atccaattaa caaatgatga aataatatct aaacaacctg aaatttatct atcttcaaat gaaaataaag atttaaatga gtttctaaca aaaaatacca atgatgttat tttagaaagt caaactcaat ttgatgaaca aatagaagac attgatcaat atgaaaaaga tcatttctta atgaggtatg aaccaaaaaa tattaaagaa atatctgata acttatttaa aagtgaaaag gatttacaaa ttgcaccaga tttacttcaa aaacaagttc atattagtgc agttacacgt aatgttttac ttgactggtt atttcttgtt tcacaagatg aatttgcaac aacaatagaa acatatttac tcactgttgc aacatttgat gattttattt ctcgcaaaac aactatccct aagtttgttc aattagttgg tgttgcttgt cttcatttat gtgttaaatt tcaagaaatc acatacccac atgttgatga atttattatt attagtaaag gtgcatatac aaaaaaccaa ttattagaga tggaaagagt gatttgtaaa acaattgatt tcagatttct tcgtccatta tcaattgatt ttttgagaag atttgataag gcagcaaatg cagatgaaat taaacatact cttggaaagt atttaatttt aattgctaca ttagaccaaa aaatgattca atgtcttcca tctcaaattg cagcagctgc tgtttatttt cagagaagaa tcaataatac tgttccttat tgggataaaa cacttattaa atactcaaca tatactgaaa aagatattct tcctattgcc caattaatga gaacgcatta tttattccaa caatcaagaa caaatcaata taaagaaatg tttaaaatgt tttcaagtag tgttaatcac acagtctcac aaattccttt catcccagaa ttttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for EnhiA.18501.a.B1.PW36444
PURIFICATION DATe: 12/10/2012
CONCENTRATION: 20.1mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 101
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV TRDTENKSEI AIKPLINQIG FRQHHVIQPP TAKINLNHSS QKSKDSQEQS NLVNTLEDKL PNLEQFVEKD DESIEKELVK LEIKPSNAFE LIQLTNDEII SKQPEIYLSS NENKDLNEFL TKNTNDVILE SQTQFDEQIE DIDQYEKDHF LMRYEPKNIK EISDNLFKSE KDLQIAPDLL QKQVHISAVT RNVLLDWLFL VSQDEFATTI ETYLLTVATF DDFISRKTTI PKSEVQLVGV ACLHLCVKFQ EITYPHVDEF IIISKGAYTK NQLLEMERVI CKTIDFRFLR PLSIDFLRRF DKAANADEIK HTLGKYLILI ATLDQKMIQC LPSQIAAAAV YFQRRINNTV PYWDKTLIKY STYTEKDILP IAQLMRTHYL FQQSRTNQYK EMFKMFSSSV NHTV
Validated NT Sequence
atggctcacc accaccacca ccatatggtt acaagagata ctgaaaataa gagcgaaatt gccattaaac cattaattaa ccaaattgga tttcgacaac atcatgtcat ccaaccacca acagccaaga ttaatcttaa tcattcatcc caaaaaagta aagacagtca agaacaaagt aatttagtaa ataccctgga agacaaatta cctaatttag aacagtttgt tgaaaaagat gatgaatcaa ttgaaaaaga attagttaaa ttggaaatta aaccaagtaa tgcgtttgaa ttaatccaat taacaaatga tgaaataata tctaaacaac ctgaaattta tctatcttca aatgaaaata aagatttaaa tgagtttcta acaaaaaata ccaatgatgt tattttagaa agtcaaactc aatttgatga acaaatagaa gacattgatc aatatgaaaa agatcatttc ttaatgaggt atgaaccaaa aaatattaaa gaaatatctg ataacttatt taaaagtgaa aaggatttac aaattgcacc agatttactt caaaaacaag ttcatattag tgcagttaca cgtaatgttt tacttgactg gttatttctt gtttcacaag atgaatttgc aacaacaata gaaacatatt tactcactgt tgcaacattt gatgatttta tttctcgcaa aacaactatc cctaaatcag aggttcaatt agttggtgtt gcttgtcttc atttatgtgt taaatttcaa gaaatcacat acccacatgt tgatgaattt attattatta gtaaaggtgc atatacaaaa aaccaattat tagagatgga aagagtgatt tgtaaaacaa ttgatttcag atttcttcgt ccattatcaa ttgatttttt gagaagattt gataaggcag caaatgcaga tgaaattaaa catactcttg gaaagtattt aattttaatt gctacattag accaaaaaat gattcaatgt cttccatctc aaattgcagc agctgctgtt tattttcaga gaagaatcaa taatactgtt ccttattggg ataaaacact tattaaatac tcaacatata ctgaaaaaga tattcttcct attgcccaat taatgagaac gcattattta ttccaacaat caagaacaaa tcaatataaa gaaatgttta aaatgttttc aagtagtgtt aatcacacag tctc
Expressed Protein Sequence
MAHHHHHHMV TRDTENKSEI AIKPLINQIG FRQHHVIQPP TAKINLNHSS QKSKDSQEQK QFVEKDDESI EKELVKLEIK PSNAFELIQL TNDEIISKQP EIYLSSNENK DLNEFLTKNT NDVILESQTQ FDEQIEDIDQ YEKDHFLMRY EPKNIKEISD NLFKSEKDLQ IAPDLLQKQV HISAVTRNVL LDWLFLVSQD EFATTIETYL LTVATFDDFI SRKTTIPKFV QLVGVACLHL CVKFQEITYP HVDEFIIISK GAYTKNQLLE MERVICKTID FRFLRPLSID FLRRFDKAAN ADEIKHTLGK YLILIATLDQ KMIQCLPSQI AAAAVYFQRR INNTVPYWDK TLIKYSTYTE KDILPIAQLM RTHYLFQQSR TNQYKEMFKM FSSSVNHTVS QIPFIPEF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTTA CAAGAGATAC TGAAAATAAG AGCGAAATTG CCATTAAACC ATTAATTAAC CAAATTGGAT TTCGACAACA TCATGTCATC CAACCACCAA CAGCCAAGAT TAATCTTAAT CATTCATCCC AAAAAAGTAA AGACAGTCAA GAACAAAAAC AGTTTGTTGA AAAAGATGAT GAATCAATTG AAAAAGAATT AGTTAAATTG GAAATTAAAC CAAGTAATGC GTTTGAATTA ATCCAATTAA CAAATGATGA AATAATATCT AAACAACCTG AAATTTATCT ATCTTCAAAT GAAAATAAAG ATTTAAATGA GTTTCTAACA AAAAATACCA ATGATGTTAT TTTAGAAAGT CAAACTCAAT TTGATGAACA AATAGAAGAC ATTGATCAAT ATGAAAAAGA TCATTTCTTA ATGAGGTATG AACCAAAAAA TATTAAAGAA ATATCTGATA ACTTATTTAA AAGTGAAAAG GATTTACAAA TTGCACCAGA TTTACTTCAA AAACAAGTTC ATATTAGTGC AGTTACACGT AATGTTTTAC TTGACTGGTT ATTTCTTGTT TCACAAGATG AATTTGCAAC AACAATAGAA ACATATTTAC TCACTGTTGC AACATTTGAT GATTTTATTT CTCGCAAAAC AACTATCCCT AAGTTTGTTC AATTAGTTGG TGTTGCTTGT CTTCATTTAT GTGTTAAATT TCAAGAAATC ACATACCCAC ATGTTGATGA ATTTATTATT ATTAGTAAAG GTGCATATAC AAAAAACCAA TTATTAGAGA TGGAAAGAGT GATTTGTAAA ACAATTGATT TCAGATTTCT TCGTCCATTA TCAATTGATT TTTTGAGAAG ATTTGATAAG GCAGCAAATG CAGATGAAAT TAAACATACT CTTGGAAAGT ATTTAATTTT AATTGCTACA TTAGACCAAA AAATGATTCA ATGTCTTCCA TCTCAAATTG CAGCAGCTGC TGTTTATTTT CAGAGAAGAA TCAATAATAC TGTTCCTTAT TGGGATAAAA CACTTATTAA ATACTCAACA TATACTGAAA AAGATATTCT TCCTATTGCC CAATTAATGA GAACGCATTA TTTATTCCAA CAATCAAGAA CAAATCAATA TAAAGAAATG TTTAAAATGT TTTCAAGTAG TGTTAATCAC ACAGTCTCAC AAATTCCTTT CATCCCAGAA TTTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT