EnhiA.18489.a

Putative uncharacterized protein

CENTER ID: EnhiA.18489.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.18489.a.B1.GE35800 full length 1 343
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_092210
RefSeq: XP_654550.1
UniProt: C4LUY6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNQEIFICGV NVGVNEIVTG KLLNEIVFNL LNQIGMSTSS TILQSNKALS LTQSQDTSYQ SIQKIEGIQS HPIERKNDRY KSALRNPSSQ INLETSCSLF TNNICTIPTT KQTNSKQTKS EIIQPTESFN PLDKRIKERA KTEMITKEIF IQEDEDFKGD LKYIKESLKY FYKWTELSQC QLIFDSTIEY DPDEYEVSSF NRLILNKENL LLIYQTNLKS HLAGIFIKDK IKVIGKNILK ENNFPFSLKC NGELDIIKKF VRSKTQGVSP LELTLTQTNQ IDLKNKPFFF IYGNRDISLK VGLSSCYSTL CDEFIDIRGT LNCCTAMGGS CFNVGRIVVV QLY
NT Sequence
atgaatcaag agatatttat ttgtggagtt aatgttggag taaatgagat agttaccggt aaattattga atgaaattgt ttttaattta ttaaatcaaa taggcatgag tacgtcttca acaattctac aaagcaataa agctttatca ttaacacaat ctcaagatac ttcttatcaa agcatacaaa aaattgaagg gattcaatct catccaatag aaaggaaaaa tgataggtat aaatcagcgc tacgaaatcc atcatctcaa attaacttag aaacttcttg ttcattgttt acaaataaca tatgtaccat tccaacaact aaacaaacta attctaaaca aacaaaatct gaaattatac aaccaactga atcatttaat ccactagata aaagaataaa agaaagagct aaaactgaaa tgattacaaa agaaattttt attcaagaag atgaagattt taaaggagat ttaaaatata tcaaagagtc acttaaatac ttttataaat ggacagaatt aagtcaatgt caattaattt ttgattctac aattgagtat gatccagatg aatatgaagt ttcaagtttt aataggttaa ttctcaataa agaaaattta ttacttattt atcaaactaa tttaaaaagt catttagcag gaatatttat taaagataaa ataaaagtta ttggaaagaa tatacttaaa gaaaataatt tcccattttc attaaaatgt aatggagaat tagatataat aaaaaagttt gttagaagca agacacaagg agtatctcct cttgaattaa ccttgacaca aacaaatcaa attgatttaa aaaataaacc atttttcttt atctatggaa atagagatat ttcattaaaa gtaggattgt cttcatgtta ctcaacttta tgtgatgagt ttattgatat tagaggaaca ttaaattgtt gtacagcaat gggaggaagt tgttttaatg ttggaagaat tgttgtagtt caattatatt aa
Details for EnhiA.18489.a.B1.GE35800
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/3/2012
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 107
Validated AA Sequence
MAHHHHHHMN QEIFICGVNV GVNEIVTGKL LNEIVFNLLN QIGMSTSSTI LQSNKALSLT QSQDTSYQSI QKIEGIQSHP IERKNDRYKS ALRNPSSQIN LETSCSLFTN NICTIPTTKQ TNSKQTKSEI IQPTESFNPL DKRIKERAKT EMITKEIFIQ EDEDFKGDLK YIKESLKYFY KWTELSQCQL IFDSTIEYDP DEYEVSSFNR LILNKENLLL IYQTNLKSHL AGIFIKDKIK VIGKNILKEN NFPFSLKCNG ELDIIKKFVR SKTQGVSPLE LTLTQTNXIX LKNKPFFFIY GNXDISLKVX XSSCYSTLCD EFXXXRXTLN XCTAXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
ttttgtnnnn nttaagaagg aganatacca tggctcacca ccaccaccac catatgaatc aagagatatt tatttgtgga gttaatgttg gagtaaatga gatagttacc ggtaaattat tgaatgaaat tgtttttaat ttattaaatc aaataggcat gagtacgtct tcaacaattc tacaaagcaa taaagcttta tcattaacac aatctcaaga tacttcttat caaagcatac aaaaaattga agggattcaa tctcatccaa tagaaaggaa aaatgatagg tataaatcag cgctacgaaa tccatcatct caaattaact tagaaacttc ttgttcattg tttacaaata acatatgtac cattccaaca actaaacaaa ctaattctaa acaaacaaaa tctgaaatta tacaaccaac tgaatcattt aatccactag ataaaagaat aaaagaaaga gctaaaactg aaatgattac aaaagaaatt tttattcaag aagatgaaga ttttaaagga gatttaaaat atatcaaaga gtcacttaaa tacttttata aatggacaga attaagtcaa tgtcaattaa tttttgattc tacaattgag tatgatccag atgaatatga agtttcaagt tttaataggt taattctcaa taaagaaaat ttattactta tttatcaaac taatttaaaa agtcatttag caggaatatt tattaaagat aaaataaaag ttattggaaa gaatatactt aaagaaaata atttcccatt ttcattaaaa tgtaatggag aattagatat aataaaaaag tttgttagaa gcaagacaca aggagtatct cctcttgaat taaccttgac acaaacaaat cnaattgant taaaaaataa accatttttc tttatctatg gaaatngaga tatttcatta aaagtagnan tgtcttcatg ttactcaact ttatgtgatg agtttantga nnntaganna acnttaaatn gntgtacagc nnnnnnnnnt ngtttnangt ngnannannn nngtanttnn nnannnnntn nnaanannnn nngnngnnan nnnnannncn nnngnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnan nnnnnnnnng
Expected Protein Sequence
MAHHHHHHMN QEIFICGVNV GVNEIVTGKL LNEIVFNLLN QIGMSTSSTI LQSNKALSLT QSQDTSYQSI QKIEGIQSHP IERKNDRYKS ALRNPSSQIN LETSCSLFTN NICTIPTTKQ TNSKQTKSEI IQPTESFNPL DKRIKERAKT EMITKEIFIQ EDEDFKGDLK YIKESLKYFY KWTELSQCQL IFDSTIEYDP DEYEVSSFNR LILNKENLLL IYQTNLKSHL AGIFIKDKIK VIGKNILKEN NFPFSLKCNG ELDIIKKFVR SKTQGVSPLE LTLTQTNQID LKNKPFFFIY GNRDISLKVG LSSCYSTLCD EFIDIRGTLN CCTAMGGSCF NVGRIVVVQL Y
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatc aagagatatt tatttgtgga gttaatgttg gagtaaatga gatagttacc ggtaaattat tgaatgaaat tgtttttaat ttattaaatc aaataggcat gagtacgtct tcaacaattc tacaaagcaa taaagcttta tcattaacac aatctcaaga tacttcttat caaagcatac aaaaaattga agggattcaa tctcatccaa tagaaaggaa aaatgatagg tataaatcag cgctacgaaa tccatcatct caaattaact tagaaacttc ttgttcattg tttacaaata acatatgtac cattccaaca actaaacaaa ctaattctaa acaaacaaaa tctgaaatta tacaaccaac tgaatcattt aatccactag ataaaagaat aaaagaaaga gctaaaactg aaatgattac aaaagaaatt tttattcaag aagatgaaga ttttaaagga gatttaaaat atatcaaaga gtcacttaaa tacttttata aatggacaga attaagtcaa tgtcaattaa tttttgattc tacaattgag tatgatccag atgaatatga agtttcaagt tttaataggt taattctcaa taaagaaaat ttattactta tttatcaaac taatttaaaa agtcatttag caggaatatt tattaaagat aaaataaaag ttattggaaa gaatatactt aaagaaaata atttcccatt ttcattaaaa tgtaatggag aattagatat aataaaaaag tttgttagaa gcaagacaca aggagtatct cctcttgaat taaccttgac acaaacaaat caaattgatt taaaaaataa accatttttc tttatctatg gaaatagaga tatttcatta aaagtaggat tgtcttcatg ttactcaact ttatgtgatg agtttattga tattagagga acattaaatt gttgtacagc aatgggagga agttgtttta atgttggaag aattgttgta gttcaattat attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat