EnhiA.18488.a

Putative uncharacterized protein

CENTER ID: EnhiA.18488.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.18488.a.B3.GE35799 region2 414 1323
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_174540
RefSeq: XP_653324.2
UniProt: C4LZK2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVNMNLIKIH YWHPNLIIHC NNAYNDTLDD QKYIIYHNLN NNGIYHAKFV LITTSRMLRL IIMSTNITNQ LIQNCWNDYY ILNIPFTSTP STTKNTDLLN LFFTTYNINL KMSLSNYDWS NVHAILSVSI PNITSHTNCW NHLFPTQPHG SCIVQTPTAM LNYNLLDTLG LDNSTFNCPF DLDSRKNSDM SYFLINNLDK NTLYKLNYIH SSLPYHFKRY FIHTLNPLAD WFILTSANLT YYAWGSKYSP SRNAELDVPL SIVDDNIPSL SDDVIVFAFN RDTGTTNITL IVEGEMSLLK FKVNGKEFDM NKIQFYCNCL FKKDSTLIFS KTDIFYTNNP DKYKVYMNEP VTIKTQEGQI IENPFANAYM EITYTDKSQL EEVEKNGENV YSTKQASLIK FDMNEDIELD KILHVYSFGF ITNKNITMTN KKSNRIVVFC DTTINNYKKE GDSEFTKSSD TDSVQINDAM ICSAGYLTVK NLKLKNCTFD IRTTTDDQSL GNSLVSGMSI VDCTFLTKQK IEAYENDSIY ISDNLKAFKS NTFLEGILLD NRLSLDKVEG KFLIKGDVST YDNNSPDSQY GYIDIVENNS ENQLKVERRT LHDDMSPYNI DLDLEKYTQV VLDDINKGSM YTSKLYSDKT SYLQWLCECQ TKCIKGVIIM EKERSNINQF IENNKNKYDI IVSEISVENQ GINLPFIDWN SYNNIQSAAL HYTRSENNDL DDSYYTKYAQ IAFMGELISH ALNSDDSRFN YYNTLIEIKD TSLKSYLDVL KDLLNLNEPQ DKNNFYLKES LVKKCFPSNF KYDESKDTIT NLKNLLTVRG IPASKYMADI YLHYLTNGDF KSIDKDTKII FANGTKMTFS EYIVKIRSDL DIVSDVIVEN DIQYFQSWED ITGDPQYSPV SAKASLEEIL QTKDINEMKR LLNEWQYKCM YLSYKNGSVI FTKDIVGNNV TFSLSKGEET LFIDDLLNMT TFKDYSDYTI NAVDYKIQRK KENKDIYILN DEDQNVKNGW VYTDKIYRLM MKYFNGSRID DKPPESTMSN EEMANIMTLI STRLTTEDEE KEGLIEILDK IEEAMNIIYT KEQKYDREDV NYSHKEYQKI RNGFEEWRNY YIPSPSEYFK TAEIMIWRPI ELEMKSVEEI RNEKYDPQYI IDYYNNIEEK YKEEKKKKWV INEQDEKNVE VIIFEDASDS EYGEMMNEIQ GARNRLQRYD SPNKLIVIMG TNYGDIYSVF GKEDLINVVP IETFFRIKNT NDIIYIPYDK RDSDTNWAID KWLFTNLYDF IFEPMMYYIN DSSPFKGLLE SINDIRYLGY VMIKQEEKGM NIK
NT Sequence
atggttaata tgaatcttat caagattcac tactggcatc ctaatcttat cattcattgt aataatgcat acaatgacac tcttgatgat cagaaataca tcatctatca taatcttaat aataatggaa tttatcatgc taaatttgtc ttaattacta catctagaat gcttagattg attattatgt ctactaacat tacaaatcaa ttaattcaaa attgttggaa tgattattac attctcaata ttcctttcac atctactcct tcaactacaa aaaacactga tcttctcaac ttattcttta ctacttataa cattaattta aaaatgtctt tatcaaatta tgattggtct aatgttcatg ctattttatc agtgagtatt cctaatatta cttctcatac aaattgttgg aatcatttat ttcctactca acctcatggc tcttgtattg ttcaaactcc aactgctatg cttaattata atttattgga tactcttgga ttagacaatt ctacctttaa ttgtccattt gatcttgact ctcgaaaaaa ttcagatatg tcttattttc ttattaataa cttagataaa aatactctct ataaattaaa ttatattcac tcttcacttc cttatcactt taaacgctat ttcatccata ctcttaatcc tcttgctgat tggttcattc ttacttcggc taatcttact tattatgctt ggggctctaa atattcccca tctagaaatg ctgaattaga tgttccatta tctattgttg atgataatat accatcttta tctgatgatg ttattgtttt tgcatttaat agagatactg gaacaacaaa cattactttg atagtagaag gagaaatgtc tcttcttaaa tttaaggtca atggcaaaga atttgatatg aataagatac aattctattg taattgtctt ttcaaaaaag actctacttt aatttttagt aaaacagata ttttttatac aaacaatcca gataagtata aagtttatat gaatgaacca gttacaatta aaacacaaga aggacaaata atagaaaatc catttgctaa tgcttatatg gaaataactt atacagataa gagtcaatta gaagaagtgg aaaaaaatgg agaaaatgtt tattctacta aacaagcatc actcattaaa tttgacatga atgaagacat agaacttgat aagatccttc atgtttattc atttggtttt attactaata aaaacattac tatgacaaac aaaaaaagta atagaatagt agtattttgt gatacaacta taaataatta taagaaggag ggagatagtg aatttactaa gagtagtgat acagactctg ttcaaataaa tgatgcaatg atatgtagtg caggatatct tactgttaaa aatctcaaat taaagaattg tacatttgat attagaacaa ctacagatga tcaatcacta ggaaatagtt tagtatcagg tatgagcata gtagattgta cttttctcac aaaacagaaa atagaggcat atgagaatga ctctatttat attagtgaca atctaaaagc atttaagagt aatacatttc tagaaggaat tctcttagat aatagactaa gtcttgataa ggtagaagga aagtttctaa tcaaaggaga tgtaagtact tatgataaca atagtcctga ttcacaatat ggatacattg atatagtaga gaataattct gaaaaccaac tcaaagtaga aagacgcaca ctacatgatg atatgtctcc atataacata gatttagatt tagagaaata tactcaagtt gtattagatg acatcaacaa aggaagtatg tatacaagta agttatactc agacaagaca tcatatcttc aatggttatg tgaatgtcaa acaaagtgta ttaaaggagt tataataatg gaaaaagaaa gaagtaatat taatcaattt atagaaaata ataagaataa atatgatatt attgttagtg aaatctctgt tgagaaccaa ggtattaatt taccttttat tgattggaac agttataata atattcaaag tgcagcacta cattatactc gttctgaaaa taatgattta gatgattcat actatacaaa atatgcacaa attgcattta tgggggaatt gatttcacat gcacttaact cagatgactc tcgttttaac tattataata ctttaattga aattaaagat acaagtctaa aaagttatct tgatgtatta aaagatttat taaatttaaa tgaacctcaa gataagaata atttttattt aaaagaatca cttgtaaaaa aatgtttccc ttctaacttt aaatatgatg aaagtaaaga tacaattact aacttaaaga atcttctaac agtaagagga attcctgcat caaaatatat ggctgatatt tatcttcact atttaacaaa tggagatttt aagagtatag ataaagacac aaaaattata tttgcaaatg gtactaaaat gacatttagt gaatatattg taaaaattag aagtgatcta gatatagtat ctgatgtaat tgttgagaat gacattcaat atttccaatc atgggaggat attacaggag atccacagta cagtccagtc agtgcaaagg catcattaga ggagatactc caaacaaagg atattaatga aatgaagaga ttgttgaatg aatggcaata taaatgcatg tatttatcat acaagaatgg aagtgttata ttcacaaaag atattgtagg aaataatgtt acatttagtc ttagtaaagg agaggaaaca ctctttattg atgatctact caatatgact acatttaagg attatagtga ttatactatt aatgcagttg attataaaat tcagagaaag aaagaaaata aggacattta cattttgaat gatgaagatc agaatgttaa gaatggatgg gtttacactg acaaaatcta tagactcatg atgaagtact ttaatggaag tagaatagat gacaaaccac ccgaaagtac aatgagtaat gaagagatgg ctaacattat gacattaata agtacaagat taactacaga agatgaagaa aaagaaggac ttattgagat tctagataag atagaagaag caatgaatat tatttataca aaagaacaga aatatgatag agaagatgtc aattatagtc ataaagaata tcaaaagata agaaatggat ttgaagaatg gagaaactat tatattccta gtccaagtga gtatttcaaa acagcagaaa ttatgatatg gagaccaatt gaattagaaa tgaaaagtgt agaagaaatt aggaatgaaa aatatgatcc tcaatatatt atagattatt acaataatat agaagagaag tataaagaag aaaagaaaaa gaaatgggtt attaatgaac aagatgaaaa gaatgtagag gtaattatat ttgaagatgc tagcgatagt gaatatggag aaatgatgaa tgaaattcaa ggagcaagaa atagacttca aagatatgat tcacctaata aattaattgt tataatggga acaaattatg gagatatcta tagtgtattt ggaaaagaag atcttattaa tgtagtacct attgaaacat tctttagaat taaaaataca aatgacataa tttatattcc atatgataaa agagatagtg atacaaattg ggcaatagat aaatggctct ttactaactt gtatgacttt atctttgagc ctatgatgta ttatatcaat gattcatctc catttaaagg attactagaa agtatcaatg acattcgata cttaggatat gttatgatta agcaggaaga aaagggaatg aatattaaat aa
Details for EnhiA.18488.a.B3.GE35799
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/6/2012
EXPECTED MW: 107kDa
OBSERVED MW: 107kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 19
Validated AA Sequence
MHVYSFGFIT NKNITMTNKK SNRIVVFCDT TINNYKKEGD SEFTKSSDTD SVQINDAMIC SAGYLTVKNL KLKNCTFDIR TTTDDQSLGN SLVSGMSIVD CTFLTKQKIE AYENDSIYIS DNLKAFKSNT FLEGILLDNR LSLDKVEGKF LIKGDVSTYD NNSPDSQYGY IDIVENNSEN Q
Validated NT Sequence
tggctcacca ccaccaccac catatgcatg tttattcatt tggttttatt actaataaaa acattactat gacaaacaaa aaaagtaata gaatagtagt attttgtgat acaactataa ataattataa gaaggaggga gatagtgaat ttactaagag tagtgataca gactctgttc aaataaatga tgcaatgata tgtagtgcag gatatcttac tgttaaaaat ctcaaattaa agaattgtac atttgatatt agaacaacta cagatgatca atcactagga aatagtttag tatcaggtat gagcatagta gattgtactt ttctcacaaa acagaaaata gaggcatatg agaatgactc tatttatatt agtgacaatc taaaagcatt taagagtaat acatttctag aaggaattct cttagataat agactaagtc ttgataaggt agaaggaaag tttctaatca aaggagatgt aagtacttat gataacaata gtcctgattc acaatatgga tacattgata tagtagagaa taattctgaa aaccaactc
Expected Protein Sequence
MAHHHHHHMH VYSFGFITNK NITMTNKKSN RIVVFCDTTI NNYKKEGDSE FTKSSDTDSV QINDAMICSA GYLTVKNLKL KNCTFDIRTT TDDQSLGNSL VSGMSIVDCT FLTKQKIEAY ENDSIYISDN LKAFKSNTFL EGILLDNRLS LDKVEGKFLI KGDVSTYDNN SPDSQYGYID IVENNSENQL KVERRTLHDD MSPYNIDLDL EKYTQVVLDD INKGSMYTSK LYSDKTSYLQ WLCECQTKCI KGVIIMEKER SNINQFIENN KNKYDIIVSE ISVENQGINL PFIDWNSYNN IQSAALHYTR SENNDLDDSY YTKYAQIAFM GELISHALNS DDSRFNYYNT LIEIKDTSLK SYLDVLKDLL NLNEPQDKNN FYLKESLVKK CFPSNFKYDE SKDTITNLKN LLTVRGIPAS KYMADIYLHY LTNGDFKSID KDTKIIFANG TKMTFSEYIV KIRSDLDIVS DVIVENDIQY FQSWEDITGD PQYSPVSAKA SLEEILQTKD INEMKRLLNE WQYKCMYLSY KNGSVIFTKD IVGNNVTFSL SKGEETLFID DLLNMTTFKD YSDYTINAVD YKIQRKKENK DIYILNDEDQ NVKNGWVYTD KIYRLMMKYF NGSRIDDKPP ESTMSNEEMA NIMTLISTRL TTEDEEKEGL IEILDKIEEA MNIIYTKEQK YDREDVNYSH KEYQKIRNGF EEWRNYYIPS PSEYFKTAEI MIWRPIELEM KSVEEIRNEK YDPQYIIDYY NNIEEKYKEE KKKKWVINEQ DEKNVEVIIF EDASDSEYGE MMNEIQGARN RLQRYDSPNK LIVIMGTNYG DIYSVFGKED LINVVPIETF FRIKNTNDII YIPYDKRDSD TNWAIDKWLF TNLYDFIFEP MMYYINDSSP FKGLLESIND IRYLGYVMIK QEEKGMNIK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcatg tttattcatt tggttttatt actaataaaa acattactat gacaaacaaa aaaagtaata gaatagtagt attttgtgat acaactataa ataattataa gaaggaggga gatagtgaat ttactaagag tagtgataca gactctgttc aaataaatga tgcaatgata tgtagtgcag gatatcttac tgttaaaaat ctcaaattaa agaattgtac atttgatatt agaacaacta cagatgatca atcactagga aatagtttag tatcaggtat gagcatagta gattgtactt ttctcacaaa acagaaaata gaggcatatg agaatgactc tatttatatt agtgacaatc taaaagcatt taagagtaat acatttctag aaggaattct cttagataat agactaagtc ttgataaggt agaaggaaag tttctaatca aaggagatgt aagtacttat gataacaata gtcctgattc acaatatgga tacattgata tagtagagaa taattctgaa aaccaactca aagtagaaag acgcacacta catgatgata tgtctccata taacatagat ttagatttag agaaatatac tcaagttgta ttagatgaca tcaacaaagg aagtatgtat acaagtaagt tatactcaga caagacatca tatcttcaat ggttatgtga atgtcaaaca aagtgtatta aaggagttat aataatggaa aaagaaagaa gtaatattaa tcaatttata gaaaataata agaataaata tgatattatt gttagtgaaa tctctgttga gaaccaaggt attaatttac cttttattga ttggaacagt tataataata ttcaaagtgc agcactacat tatactcgtt ctgaaaataa tgatttagat gattcatact atacaaaata tgcacaaatt gcatttatgg gggaattgat ttcacatgca cttaactcag atgactctcg ttttaactat tataatactt taattgaaat taaagataca agtctaaaaa gttatcttga tgtattaaaa gatttattaa atttaaatga acctcaagat aagaataatt tttatttaaa agaatcactt gtaaaaaaat gtttcccttc taactttaaa tatgatgaaa gtaaagatac aattactaac ttaaagaatc ttctaacagt aagaggaatt cctgcatcaa aatatatggc tgatatttat cttcactatt taacaaatgg agattttaag agtatagata aagacacaaa aattatattt gcaaatggta ctaaaatgac atttagtgaa tatattgtaa aaattagaag tgatctagat atagtatctg atgtaattgt tgagaatgac attcaatatt tccaatcatg ggaggatatt acaggagatc cacagtacag tccagtcagt gcaaaggcat cattagagga gatactccaa acaaaggata ttaatgaaat gaagagattg ttgaatgaat ggcaatataa atgcatgtat ttatcataca agaatggaag tgttatattc acaaaagata ttgtaggaaa taatgttaca tttagtctta gtaaaggaga ggaaacactc tttattgatg atctactcaa tatgactaca tttaaggatt atagtgatta tactattaat gcagttgatt ataaaattca gagaaagaaa gaaaataagg acatttacat tttgaatgat gaagatcaga atgttaagaa tggatgggtt tacactgaca aaatctatag actcatgatg aagtacttta atggaagtag aatagatgac aaaccacccg aaagtacaat gagtaatgaa gagatggcta acattatgac attaataagt acaagattaa ctacagaaga tgaagaaaaa gaaggactta ttgagattct agataagata gaagaagcaa tgaatattat ttatacaaaa gaacagaaat atgatagaga agatgtcaat tatagtcata aagaatatca aaagataaga aatggatttg aagaatggag aaactattat attcctagtc caagtgagta tttcaaaaca gcagaaatta tgatatggag accaattgaa ttagaaatga aaagtgtaga agaaattagg aatgaaaaat atgatcctca atatattata gattattaca ataatataga agagaagtat aaagaagaaa agaaaaagaa atgggttatt aatgaacaag atgaaaagaa tgtagaggta attatatttg aagatgctag cgatagtgaa tatggagaaa tgatgaatga aattcaagga gcaagaaata gacttcaaag atatgattca cctaataaat taattgttat aatgggaaca aattatggag atatctatag tgtatttgga aaagaagatc ttattaatgt agtacctatt gaaacattct ttagaattaa aaatacaaat gacataattt atattccata tgataaaaga gatagtgata caaattgggc aatagataaa tggctcttta ctaacttgta tgactttatc tttgagccta tgatgtatta tatcaatgat tcatctccat ttaaaggatt actagaaagt atcaatgaca ttcgatactt aggatatgtt atgattaagc aggaagaaaa gggaatgaat attaaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t