EnhiA.18485.a

Calreticulin, putative

CENTER ID: EnhiA.18485.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.18485.a.B2.GE35795 mature protein 14 389
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.18485.a.B2.PS01621 mature protein 14 389

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_136160
OrthoMCL: OG5_129181
RefSeq: XP_655241.1
UniProt: C4M296

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFTLFLLIAL SSAKVYFHET FENRDKWIDS TSSGKALGPF KIVSGKWYGD ANNKGLQTSE DNKFYIAAAK LDEEFSNKDK NLIVQYNLKF EQGIDCGGGY IKLLPKKSIE SEEKFTPESE YNIMFGPDVC GGSKRTHVIM NYKGKNNLIR KEIKCESDDI SHLYTLIIRP NNTYVVKIDG VEKQEGKFDE DWDMLAPKEI DDPNVSKPAD WVDEKEIDDP NDKKPEGWDD IPKTIVDPNA KKPEEWNDED DGEWEAPTIE NPEYKGEWKP KRIPNPAYKG EWVHPQIANP DYVYDPELYK YDSFAYIGID VWQVKAGTIY DDILITDDIE EAEKEAKVIL ERNAAEKKMR DEIKEAEKQK EEEAKKEAEK QKEEETKEEI KKEENKEEL
NT Sequence
atgtttactc ttttccttct cattgcacta agcagtgcta aggtttattt ccatgaaaca tttgagaatc gagacaaatg gatcgattca acatcttcag gaaaggcatt aggaccattt aaaatagtaa gtgggaaatg gtatggagat gctaataata aaggacttca aacatcagaa gacaacaaat tttatattgc agcagctaaa cttgatgaag agtttagtaa taaagataaa aatttgattg ttcaatacaa tcttaaattt gaacaaggaa ttgattgtgg aggaggatat attaaattac ttccaaagaa atcaattgaa agtgaagaga aatttacacc tgaatctgaa tataatatta tgtttggacc agatgtatgt ggaggatcaa aaagaacaca tgttattatg aattataaag gaaagaataa tttaattaga aaagaaatta aatgtgaatc agatgatatt tcacatttat acacattaat tattagacca aataatacct atgtagttaa aattgatgga gtagaaaaac aagaaggaaa gtttgatgaa gattgggata tgttagcacc aaaagaaatt gatgatccaa atgtctcaaa accagctgat tgggttgatg aaaaggaaat tgatgatcca aatgataaga aaccagaagg atgggatgat attcctaaaa ctattgttga tccaaatgct aagaaaccag aagaatggaa tgatgaagat gatggagaat gggaagcacc aacaattgaa aatccagaat ataaaggaga atggaagcct aagagaattc ctaatccagc atataaagga gaatgggtac atccacaaat tgcaaatcca gactatgttt atgacccaga attgtataaa tatgattcat ttgcttatat tggaattgat gtttggcaag ttaaagcagg aactatttat gatgatattt taattaccga tgatattgaa gaagcagaaa aagaagctaa agttatttta gaaagaaatg cagctgaaaa gaaaatgaga gacgaaataa aagaagcaga aaaacaaaaa gaagaagaag ctaaaaaaga agcagaaaaa caaaaagaag aagagactaa agaagaaatt aagaaagaag aaaacaaaga agagctttaa
Details for EnhiA.18485.a.B2.GE35795
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/3/2012
EXPECTED MW: 44kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMK VYFHETFENR DKWIDSTSSG KALGPFKIVS GKWYGDANNK GLQTSEDNKF YIAAAKLDEE FSNKDKNLIV QYNLKFEQGI DCGGGYIKLL PKKSIESEEK FTPESEYNIM FGPDVCGGSK RTHVIMNYKG KNNLIRKEIK CESDDISHLY TLIIRPNNTY VVKIDGVEKQ EGKFDEDWDM LAPKEIDDPN VSKPADWVDE KEIDDPNDKK PEGWDDIPKT IVDPNAKKPE EWNDEDDGEW EAPTIENPEY KGEWKPKRIP NPAYKGEWVH PQIANPDYVY DPELYKYDSF AYIGIDVWQV KAGTIYDDIL ITDDIEEAEK EAKVILERNA AEKKMRDEIK EAEKQKEEEA KKEAEKQKEE ETKE
Validated NT Sequence
atggctcacc accaccacca ccatatgaag gtttatttcc atgaaacatt tgagaatcga gacaaatgga tcgattcaac atcttcagga aaggcattag gaccatttaa aatagtaagt gggaaatggt atggagatgc taataataaa ggacttcaaa catcagaaga caacaaattt tatattgcag cagctaaact tgatgaagag tttagtaata aagataaaaa tttgattgtt caatacaatc ttaaatttga acaaggaatt gattgtggag gaggatatat taaattactt ccaaagaaat caattgaaag tgaagagaaa tttacacctg aatctgaata taatattatg tttggaccag atgtatgtgg aggatcaaaa agaacacatg ttattatgaa ttataaagga aagaataatt taattagaaa agaaattaaa tgtgaatcag atgatatttc acatttatac acattaatta ttagaccaaa taatacctat gtagttaaaa ttgatggagt agaaaaacaa gaaggaaagt ttgatgaaga ttgggatatg ttagcaccaa aagaaattga tgatccaaat gtctcaaaac cagctgattg ggttgatgaa aaggaaattg atgatccaaa tgataagaaa ccagaaggat gggatgatat tcctaaaact attgttgatc caaatgctaa gaaaccagaa gaatggaatg atgaagatga tggagaatgg gaagcaccaa caattgaaaa tccagaatat aaaggagaat ggaagcctaa gagaattcct aatccagcat ataaaggaga atgggtacat ccacaaattg caaatccaga ctatgtttat gacccagaat tgtataaata tgattcattt gcttatattg gaattgatgt ttggcaagtt aaagcaggaa ctatttatga tgatatttta attaccgatg atattgaaga agcagaaaaa gaagctaaag ttattttaga aagaaatgca gctgaaaaga aaatgagaga cgaaataaaa gaagcagaaa aacaaaaaga agaagaagct aaaaaagaag cagaaaaaca aaaagaagaa gagactaaag aagaa
Expected Protein Sequence
MAHHHHHHMK VYFHETFENR DKWIDSTSSG KALGPFKIVS GKWYGDANNK GLQTSEDNKF YIAAAKLDEE FSNKDKNLIV QYNLKFEQGI DCGGGYIKLL PKKSIESEEK FTPESEYNIM FGPDVCGGSK RTHVIMNYKG KNNLIRKEIK CESDDISHLY TLIIRPNNTY VVKIDGVEKQ EGKFDEDWDM LAPKEIDDPN VSKPADWVDE KEIDDPNDKK PEGWDDIPKT IVDPNAKKPE EWNDEDDGEW EAPTIENPEY KGEWKPKRIP NPAYKGEWVH PQIANPDYVY DPELYKYDSF AYIGIDVWQV KAGTIYDDIL ITDDIEEAEK EAKVILERNA AEKKMRDEIK EAEKQKEEEA KKEAEKQKEE ETKEEIKKEE NKEEL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagg tttatttcca tgaaacattt gagaatcgag acaaatggat cgattcaaca tcttcaggaa aggcattagg accatttaaa atagtaagtg ggaaatggta tggagatgct aataataaag gacttcaaac atcagaagac aacaaatttt atattgcagc agctaaactt gatgaagagt ttagtaataa agataaaaat ttgattgttc aatacaatct taaatttgaa caaggaattg attgtggagg aggatatatt aaattacttc caaagaaatc aattgaaagt gaagagaaat ttacacctga atctgaatat aatattatgt ttggaccaga tgtatgtgga ggatcaaaaa gaacacatgt tattatgaat tataaaggaa agaataattt aattagaaaa gaaattaaat gtgaatcaga tgatatttca catttataca cattaattat tagaccaaat aatacctatg tagttaaaat tgatggagta gaaaaacaag aaggaaagtt tgatgaagat tgggatatgt tagcaccaaa agaaattgat gatccaaatg tctcaaaacc agctgattgg gttgatgaaa aggaaattga tgatccaaat gataagaaac cagaaggatg ggatgatatt cctaaaacta ttgttgatcc aaatgctaag aaaccagaag aatggaatga tgaagatgat ggagaatggg aagcaccaac aattgaaaat ccagaatata aaggagaatg gaagcctaag agaattccta atccagcata taaaggagaa tgggtacatc cacaaattgc aaatccagac tatgtttatg acccagaatt gtataaatat gattcatttg cttatattgg aattgatgtt tggcaagtta aagcaggaac tatttatgat gatattttaa ttaccgatga tattgaagaa gcagaaaaag aagctaaagt tattttagaa agaaatgcag ctgaaaagaa aatgagagac gaaataaaag aagcagaaaa acaaaaagaa gaagaagcta aaaaagaagc agaaaaacaa aaagaagaag agactaaaga agaaattaag aaagaagaaa acaaagaaga gctttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for EnhiA.18485.a.B2.PS01621
PURIFICATION DATe: 8/13/2012
CONCENTRATION: 45.8mg/ml
OBSERVED MW: 49kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK VYFHETFENR DKWIDSTSSG KALGPFKIVS GKWYGDANNK GLQTSEDNKF YIAAAKLDEE FSNKDKNLIV QYNLKFEQGI DCGGGYIKLL PKKSIESEEK FTPESEYNIM FGPDVCGGSK RTHVIMNYKG KNNLIRKEIK CESDDISHLY TLIIRPNNTY VVKIDGVEKQ EGKFDEDWDM LAPKEIDDPN VSKPADWVDE KEIDDPNDKK PEGWDDIPKT IVDPNAKKPE EWNDEDDGEW EAPTIENPEY KGEWKPKRIP NPAYKGEWVH PQIANPDYVY DPELYKYDSF AYIGIDVWQV KAGTIYDDIL ITDDIEEAEK EAKVILERNA AEKKMRDEIK EAEKQKEEEA KKEAEKQKEE ETKE
Validated NT Sequence
atggctcacc accaccacca ccatatgaag gtttatttcc atgaaacatt tgagaatcga gacaaatgga tcgattcaac atcttcagga aaggcattag gaccatttaa aatagtaagt gggaaatggt atggagatgc taataataaa ggacttcaaa catcagaaga caacaaattt tatattgcag cagctaaact tgatgaagag tttagtaata aagataaaaa tttgattgtt caatacaatc ttaaatttga acaaggaatt gattgtggag gaggatatat taaattactt ccaaagaaat caattgaaag tgaagagaaa tttacacctg aatctgaata taatattatg tttggaccag atgtatgtgg aggatcaaaa agaacacatg ttattatgaa ttataaagga aagaataatt taattagaaa agaaattaaa tgtgaatcag atgatatttc acatttatac acattaatta ttagaccaaa taatacctat gtagttaaaa ttgatggagt agaaaaacaa gaaggaaagt ttgatgaaga ttgggatatg ttagcaccaa aagaaattga tgatccaaat gtctcaaaac cagctgattg ggttgatgaa aaggaaattg atgatccaaa tgataagaaa ccagaaggat gggatgatat tcctaaaact attgttgatc caaatgctaa gaaaccagaa gaatggaatg atgaagatga tggagaatgg gaagcaccaa caattgaaaa tccagaatat aaaggagaat ggaagcctaa gagaattcct aatccagcat ataaaggaga atgggtacat ccacaaattg caaatccaga ctatgtttat gacccagaat tgtataaata tgattcattt gcttatattg gaattgatgt ttggcaagtt aaagcaggaa ctatttatga tgatatttta attaccgatg atattgaaga agcagaaaaa gaagctaaag ttattttaga aagaaatgca gctgaaaaga aaatgagaga cgaaataaaa gaagcagaaa aacaaaaaga agaagaagct aaaaaagaag cagaaaaaca aaaagaagaa gagactaaag aagaa
Expressed Protein Sequence
MAHHHHHHMK VYFHETFENR DKWIDSTSSG KALGPFKIVS GKWYGDANNK GLQTSEDNKF YIAAAKLDEE FSNKDKNLIV QYNLKFEQGI DCGGGYIKLL PKKSIESEEK FTPESEYNIM FGPDVCGGSK RTHVIMNYKG KNNLIRKEIK CESDDISHLY TLIIRPNNTY VVKIDGVEKQ EGKFDEDWDM LAPKEIDDPN VSKPADWVDE KEIDDPNDKK PEGWDDIPKT IVDPNAKKPE EWNDEDDGEW EAPTIENPEY KGEWKPKRIP NPAYKGEWVH PQIANPDYVY DPELYKYDSF AYIGIDVWQV KAGTIYDDIL ITDDIEEAEK EAKVILERNA AEKKMRDEIK EAEKQKEEEA KKEAEKQKEE ETKEEIKKEE NKEEL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGG TTTATTTCCA TGAAACATTT GAGAATCGAG ACAAATGGAT CGATTCAACA TCTTCAGGAA AGGCATTAGG ACCATTTAAA ATAGTAAGTG GGAAATGGTA TGGAGATGCT AATAATAAAG GACTTCAAAC ATCAGAAGAC AACAAATTTT ATATTGCAGC AGCTAAACTT GATGAAGAGT TTAGTAATAA AGATAAAAAT TTGATTGTTC AATACAATCT TAAATTTGAA CAAGGAATTG ATTGTGGAGG AGGATATATT AAATTACTTC CAAAGAAATC AATTGAAAGT GAAGAGAAAT TTACACCTGA ATCTGAATAT AATATTATGT TTGGACCAGA TGTATGTGGA GGATCAAAAA GAACACATGT TATTATGAAT TATAAAGGAA AGAATAATTT AATTAGAAAA GAAATTAAAT GTGAATCAGA TGATATTTCA CATTTATACA CATTAATTAT TAGACCAAAT AATACCTATG TAGTTAAAAT TGATGGAGTA GAAAAACAAG AAGGAAAGTT TGATGAAGAT TGGGATATGT TAGCACCAAA AGAAATTGAT GATCCAAATG TCTCAAAACC AGCTGATTGG GTTGATGAAA AGGAAATTGA TGATCCAAAT GATAAGAAAC CAGAAGGATG GGATGATATT CCTAAAACTA TTGTTGATCC AAATGCTAAG AAACCAGAAG AATGGAATGA TGAAGATGAT GGAGAATGGG AAGCACCAAC AATTGAAAAT CCAGAATATA AAGGAGAATG GAAGCCTAAG AGAATTCCTA ATCCAGCATA TAAAGGAGAA TGGGTACATC CACAAATTGC AAATCCAGAC TATGTTTATG ACCCAGAATT GTATAAATAT GATTCATTTG CTTATATTGG AATTGATGTT TGGCAAGTTA AAGCAGGAAC TATTTATGAT GATATTTTAA TTACCGATGA TATTGAAGAA GCAGAAAAAG AAGCTAAAGT TATTTTAGAA AGAAATGCAG CTGAAAAGAA AATGAGAGAC GAAATAAAAG AAGCAGAAAA ACAAAAAGAA GAAGAAGCTA AAAAAGAAGC AGAAAAACAA AAAGAAGAAG AGACTAAAGA AGAAATTAAG AAAGAAGAAA ACAAAGAAGA GCTTTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT