SSGCID: Target Page

EnhiA.18473.a

Putative uncharacterized protein

CENTER ID:	EnhiA.18473.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.18473.a.B5.GE35774	region4		1021	1499

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_010740
RefSeq:	XP_653055.2
UniProt:	C4LYW1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKEVKERNEE SNETSLWKKS SIMSINECSI PSEGSFKKCL DNINDYLMKR INIHLIGHFI VCEIDKTERT NINECLLYLK NIGHLSYIKL SPKVKQLCYC QYPEIVSSII SMFPDYIVLN DINEACYNEV NDGETYLEYI KRVYCSICNK DSLLGAQLKY MKYSHENCEH LLHIIQAAKN LSNIELLQEM MKDEEYRDDS LQLISNNFSN EFILSMLVDI TKYYLNLSLK QQLNKEVIFF IHCCLSHLLN RNPPCLVELS LFLYQCDQID IHLRNDIVSL SLFNSSSDEV IEQIKHIIDI SESVKFDLQV TLIDKLLRMN SFKPTDSEPQ FDELVQIIND VIDQTQDNAV ITCLFILFDC IIQYTELFDA DQVKPTFSKL IKYMKQHFCL AFIKMMINEY INISIFTCSF NTYLQDMVDS IKSDLSINFI KFDYKYKQKE PFNNEIDFEE INDSIKKDGY KWFAIQKVMN KKQISRNSDY RICFDSILKK YIPIQCIPNF IHRYNKYFSF NNLEITVQIT QEELKERQEY SLDSLCQILL HFNLIYANSS KRNQNNFIEK EEIMKILDIT FDLINYVASN NTHIITRIFN NSIDKETDNT LQKINCIIDL MTETIGGFPE DMFIKEFMKK KTIRNGLLYL LFFLQQKETP LRKRDREWYY KHQRKIISLL WNKIPPLKSN KDKLIYHYIL KSIPSFIEHF NCDNTFKQTI INFLLPEVVS FKIISQLFNQ GIFVDLNEID SFLGHPLINH TPKEIKDILI QEFEKSCSIK RKLLPVVGYI YDDIDLTRFN ENKHFTAKAS FVKVFKGSSL IIKGCEETLI SLIDRVSLID IQLYRPMIEL CNKDILKEIL LSKNTLGISL TSALMLCQIA DFIQFPLQIH SIGILNFHQD ENEKYDIYLD NLDLVSMLIQ CLVKRLDATE YNEEMNVVRL IMKFINNNTV LLFRKYQQRI LQIILRNSLN KQDVLMLSTI YKEGYIDINT EEGSIIKMLF HRSNISELDI SDIIHFGLYQ NKENICLFEE PRKQCNEPMN KNNDIFNQYI YLTLKGKSME IKNDTDDILF YQLCQESFIT KRTKGMLEGN WDIKWKEDCL NKIRKVIKII SDQVKDTPQE HDKISNKEEK MEEENSEIKE LKELVHILKI NEEIEDIVKR FIKYSEKNKI DKMKEMKSLM IMYQEILTEQ EMKDGCNTII SFMNKFKGMN IKIDYQEDKK EEETEEENVH VIDIPGLGVV PIPQGFDTET LMQLPEEYRI EAVMNYYQEH NIPRIRRSEE RRGIQLSHNI RKFPCEEFKQ RRINKLEMNS NTLKKTWISS SSKTVPEFNI KKALSIYFVC TEDERKIILQ KINSLFCNSQ INFNVVYNNF MKYIVNDLFI EILQSKVDEK MIRYWQLVND VNQQKDSEIS SEINEMIIDN LCMMVEANMK NSIILNAVVY LPQILEIEEI KINKESYILK LLSIFGRYYR NRSHSNQLHF ESKTQRLIDK CLKKNIQVLH EKELNQDYLL DLYYMLLTLV PSTLNEQEEI ERSTSPFLVR LVNQRIPLRN LEFGILPPQE YILGDGEYLQ LPRTFGELGV NEIRQRIDPQ YHIVREYNDG SNDDNDEMQF NVESDHQNNS EVSQHDTITT QPLNQIKELQ TLLENISKKN ISPSLVQEKV NQLTQDILME EEEPQQIEKK EEKEMEEEYV IIENYSNLLN YVKKYHNELV CRVVLLLLSY VIKEEYNEIV KDIVIEMKWF IEKELRLNRF ALSSSLRFLS KHREFLPFEM KYQDLIKEIG KHKIYNHFMK IIIDRNNVFN SLFKQIKHSE KWDRLFKIEF KHEQGNDNGG LLKECYSLLV KELSNGIEDL FIIENNYIVP NYQCFKKNQF IFIGKCLAKM IYDGFTSDFH LALFVLRLLL SKRNELKDVK MFDKEMSQQI DNLFLEDVTE WGLTFTETER INNKIHEVEL KTNGANILVT QENKKEYIDL LLQYKFNTRI EKQIKWIKKG FYSLIDESTI QLFDEKELDR IICGNNFDIE DLKRNVVLDG YTKESLQIIW LWEIINEMEN SLKGKFIQFV TGSSRAPIGG FVQLKSSHGT PLPFTIASIH YDNPDSMMPT AHTCANRLEI PFYSNKDILK SKLTIAIQEC CNYEFD

NT Sequence

atgaaagaag ttaaagagag aaatgaagaa agcaatgaaa cgtcattgtg gaaaaagtca agtataatgt ctatcaatga atgttcaata ccaagtgaag gttcttttaa aaaatgctta gataatatta atgattattt aatgaaacga ataaatattc atttgattgg gcattttatt gtttgtgaaa ttgataagac agaaagaact aatattaatg aatgtttgtt atacctcaaa aatattggac atctttctta tattaaatta tctcccaaag taaaacaact ttgttattgt caatatcctg aaattgtttc ttcaataatt tcaatgttcc cagattatat agtattaaat gatataaatg aagcttgtta taatgaagta aatgatggtg aaacgtattt agaatatata aaaagagttt attgttcaat atgtaataag gatagtttac ttggagctca attaaaatat atgaaatact cccatgaaaa ttgtgaacat cttcttcata ttattcaagc agcaaaaaat ttaagtaata ttgaattatt acaagaaatg atgaaagatg aagagtatag agatgattct ttacaactta tttctaataa tttttctaat gaatttattt tgagtatgtt agtagatata acaaagtatt atcttaattt atcattgaaa caacaattga ataaagaagt aatatttttt attcattgtt gtttgtctca tttattaaat agaaatcctc catgtttagt tgagttatct ttatttttat atcaatgtga tcaaattgat attcatttaa gaaatgatat tgtttctctt tcattattta attcttcatc tgatgaagtg attgaacaga ttaaacatat tattgatatt tcagagtcgg ttaaatttga tttacaagtt actcttattg ataaattatt acgtatgaat agttttaaac caacagactc tgaaccacaa tttgatgaat tagttcaaat aattaatgat gtaatagatc aaacacagga caatgctgtt attacttgtt tatttatctt atttgattgt attattcaat atacagaatt atttgatgct gatcaagtaa aaccaacatt ttctaaattg ataaagtata tgaaacaaca cttttgttta gcattcatta agatgatgat taatgaatat ataaatatat caatatttac atgttctttt aatacttatc ttcaagacat ggttgattct attaaatcgg atttatcaat taattttatt aagtttgact ataaatataa acaaaaagaa ccatttaata atgaaattga ctttgaagaa attaatgatt caattaaaaa agatggatat aaatggtttg caattcaaaa agtaatgaat aaaaaacaaa taagtagaaa tagtgattat agaatttgtt ttgattctat tttaaagaaa tatattccaa tacaatgtat tccaaacttt attcatagat acaataaata ttttagtttt aataatttag aaattactgt tcaaataaca caggaagaat taaaagaaag acaggaatat tcacttgata gtttatgtca aatattactt cattttaatc ttatttatgc taattcatca aaaagaaatc aaaacaattt tattgaaaaa gaagaaatta tgaagatact tgatattact tttgatttaa taaattatgt agctagtaat aatactcata taatcactag aatatttaat aattctattg ataaagaaac agataatact ttacaaaaaa ttaattgtat tattgattta atgactgaaa ctattggagg atttccagaa gatatgttta ttaaagaatt tatgaaaaag aaaacaatta gaaatggatt gttatattta ttattctttc ttcaacaaaa agaaactcca cttagaaaac gagatagaga atggtattat aaacatcaaa gaaaaattat ttctttattg tggaataaaa taccaccact caaaagtaat aaagataaat taatatatca ttatattctt aaatcaattc cttcttttat tgaacatttc aattgtgaca atacttttaa acaaactatt attaattttc ttcttcctga agtagtatca tttaaaataa tttcacagtt atttaatcaa ggaatatttg ttgatttaaa tgaaattgat tctttcttgg gtcatccatt aatcaatcat acaccaaaag aaataaaaga tattttaatt caagaattcg aaaaaagttg ttcaattaaa cgtaaattac ttcctgtagt gggatatatt tatgatgata tagatttaac acgatttaat gagaataaac attttacagc aaaagcttct tttgttaagg tatttaaagg tagtagtctt attattaaag gatgtgaaga gactttaatt tctcttattg atagagtttc tcttattgat attcaattat atcgaccaat gattgaatta tgtaataaag atatattaaa agaaatcctt ttatcaaaaa atactttggg gattagttta actagtgcat taatgttatg tcaaatagct gatttcattc aatttccatt acaaatacat agtattggaa tacttaattt ccatcaagat gaaaatgaaa aatatgatat ttatcttgat aatcttgatt tggtttcaat gttgattcag tgtttagtta aaagattaga tgctacagaa tataatgaag aaatgaatgt agttcgatta atcatgaaat ttattaataa taatacagta ttattattcc gtaaatacca acaaagaatt cttcaaatta tacttcgtaa ttcattaaac aaacaagatg ttttaatgct ttctactatt tacaaagaag gatatattga tatcaatact gaggaaggaa gtattattaa aatgttattt catagaagta atatttctga acttgacata tctgatatta tacattttgg attatatcag aataaagaaa acatttgttt atttgaagaa cctagaaaac aatgtaatga acctatgaat aaaaataacg atatatttaa ccaatatatt tacttaacat tgaaaggaaa atcaatggaa ataaagaatg atactgatga tattttattt tatcaattat gtcaagaaag ttttataact aaaagaacaa aagggatgtt ggaagggaat tgggatatta aatggaagga agattgttta aataaaatta gaaaggttat taaaattatt agtgaccaag ttaaagacac accacaagaa catgataaaa tttcaaacaa agaagaaaaa atggaagaag aaaatagtga aataaaagag ttaaaagaac ttgtacatat tcttaagatt aatgaagaaa ttgaagatat tgtaaaaaga tttatcaaat atagtgagaa aaataagatt gataaaatga aagaaatgaa atcattaatg attatgtatc aagaaattct aacagaacaa gagatgaaag atggatgtaa tacaattatt agtttcatga ataaatttaa aggaatgaat ataaaaatcg actatcaaga agataaaaag gaagaagaaa cagaagaaga aaatgttcat gttattgata ttcctgggtt aggtgtagtt cctattcctc aaggatttga tactgaaaca ttaatgcaat tacctgaaga atatagaata gaagcagtaa tgaattatta tcaagaacat aatattccaa gaatacgaag aagtgaagag agaagaggta ttcaattatc acataatata agaaaattcc cgtgtgaaga atttaaacaa agaaggataa ataaattgga aatgaattca aatacactca aaaaaacatg gataagttct agttcaaaga ctgtacctga atttaacatc aaaaaagcat tgagtatata ttttgtttgt acagaagatg aacgtaaaat tatattacag aagataaatt cattattctg taattcacaa attaacttca atgttgtata taataatttt atgaaatata ttgtcaatga tttatttatt gaaattcttc aaagtaaagt tgatgagaaa atgattagat attggcaatt agtcaatgat gttaatcaac aaaaagattc agaaatatct tcagaaatta atgaaatgat aattgataat ttatgtatga tggttgaagc taacatgaaa aattcaataa ttctcaatgc agttgtatat ttacctcaaa tattagaaat agaggaaatt aaaattaata aagaaagtta catattaaaa ttattatcta tctttggaag gtattatcga aatagatcac attctaacca acttcatttt gaatcaaaaa cacaacgtct tattgataaa tgtttaaaga aaaatataca agttttacat gaaaaagaat taaatcaaga ctatttacta gacttgtatt atatgttatt aacactagtt ccttctacat taaatgaaca agaagaaata gaacgaagta catcaccatt tcttgttaga ttagtaaacc aaagaatacc attaagaaat ttagaatttg gaattttacc accacaagaa tatattcttg gtgatggaga atatttgcaa ttaccacgaa cttttggaga attaggtgtc aatgaaatac gccaaagaat tgatccacaa tatcatattg taagagaata taatgacgga agtaatgatg ataatgatga aatgcaattt aatgtagaaa gtgatcatca aaacaattcc gaagtgtctc aacatgatac tataacaaca caaccactaa atcaaataaa ggaattacaa acacttttag aaaatatttc aaaaaagaat atttctccat cacttgttca agaaaaggta aatcaattaa cccaagatat tttgatggaa gaagaagagc ctcaacaaat agaaaaaaaa gaagaaaaag aaatggaaga agaatatgta atcattgaaa attattcaaa tcttttgaat tatgttaaaa aatatcataa cgaattagtg tgtcgagttg tgttattatt acttagttat gttataaaag aagagtataa tgaaatagtt aaagatattg taatagaaat gaaatggttt attgaaaaag aattaagatt aaatagattt gcattaagta gctctttaag atttcttagt aaacatagag aatttcttcc atttgaaatg aagtatcaag acttaataaa agaaattgga aaacataaga tttataacca ttttatgaaa attattattg atagaaataa tgtatttaac tctttattta aacaaataaa acattcagaa aaatgggata gattatttaa aatagaattt aaacatgaac aaggaaatga taatggagga ttattgaaag aatgttatag tcttcttgtt aaagaattaa gtaatggaat agaagattta tttattatag aaaataatta tattgttcca aattatcaat gttttaagaa aaatcaattt atatttattg gaaaatgtct tgcaaaaatg atttatgatg gatttacaag tgattttcat ttagcattat ttgtattaag attattgtta agtaaaagaa atgaattaaa agatgtcaaa atgtttgata aagaaatgag tcaacaaata gataatttgt ttttagaaga tgttactgaa tgggggttaa catttacaga aacagaaaga attaataata aaattcatga agttgaacta aaaacaaatg gagcaaatat tcttgttact caagaaaata aaaaagaata tattgattta cttcttcaat ataaatttaa tacaagaata gaaaaacaaa taaaatggat taaaaaaggg ttttattctc ttattgatga atcaactatt caactatttg atgaaaaaga attggataga attatttgtg gaaataattt tgatattgaa gaccttaaaa gaaatgttgt acttgatgga tatacaaagg aatcacttca aattatatgg ttatgggaaa ttattaatga aatggaaaat tctttaaaag gaaaatttat tcaatttgtt actggttctt ctagagcacc tatcggtgga tttgttcaat taaaaagtag tcatggaact ccattaccat ttactattgc ctctattcat tatgataatc ctgattctat gatgccaaca gcacatacat gtgctaatag gttagaaatt ccattttatt caaacaaaga catattaaaa tcaaaattaa caatagcaat acaagagtgt tgtaactatg aatttgatta a

Details for EnhiA.18473.a.B5.GE35774

HARVESTED ON:	7/31/2012
SEQUENCED ON:	8/3/2012
EXPECTED MW:	58kDa
OBSERVED MW:	65kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	56

Validated AA Sequence

Validated NT Sequence

cnannnnnnn tttntnnnnt ttaagangga gatataccat ggctcaccac caccaccacc atatgcctag aaaacaatgt aatgaaccta tgaataaaaa taacgatata tttaaccaat atatttactt aacattgaaa ggaaaatcaa tggaaataaa gaatgatact gatgatattt tattttatca attatgtcaa gaaagtttta taactaaaag aacaaaaggg atgttggaag ggaattggga tattaaatgg aaggaagatt gtttaaataa aattagaaag gttattaaaa ttattagtga ccaagttaaa gacacaccac aagaacatga taaaatttca aacaaagaag aaaaaatgga agaagaaaat agtgaaataa aagagttaaa agaacttgta catattctta agattaatga agaaattgaa gatattgtaa aaagatttat caaatatagt gagaaaaata agattgataa aatgaaagaa atgaaatcat taatgattat gtatcaagaa attctaacag aacaagagat gaaagatgga tgtaatacaa ttattagttt catgaataaa tttaaaggaa tgaatataaa aatcgactat caagaagata aaaaggaaga agaaacagaa gaagaaaatg ttcatgttat tgatattcct gggttaggtg tagttcctat tcctcaagga tttgatactg aaacattaat gcaattacct gaagaatata gaatagaagc agtaatgaat tattatcaag aacataatat tccaagaata cgaagaagtg aagagagaag aggtattcaa ttatcacata atataagaaa aattcccgng tgaagaattt aaacaaagaa nggataaata aattgnaant gaattcnaat acactcaaaa aaacatggnt aagttctagt tcaaagactg tacctgaatt tnacatcaaa aaagcattga gtatatattt tgnttgnacn nanangaacg taaannatat tacagcatta ttctgnaatt cnnnantnac ntcnnngntg nnnntaanaa ntttnnnnan nnnntnnnna ngatttnttt nnnnnantnn tnnnngnnaa nntannnnaa annnnnnnna nntngnnnnn nnnnnnnnnn ntnnnnnnnn annnnnnnnn nnnnnnnnnn nnaannnnnn nnnnnnannn nnnnnnnnnn a

Expected Protein Sequence

MAHHHHHHMP RKQCNEPMNK NNDIFNQYIY LTLKGKSMEI KNDTDDILFY QLCQESFITK RTKGMLEGNW DIKWKEDCLN KIRKVIKIIS DQVKDTPQEH DKISNKEEKM EEENSEIKEL KELVHILKIN EEIEDIVKRF IKYSEKNKID KMKEMKSLMI MYQEILTEQE MKDGCNTIIS FMNKFKGMNI KIDYQEDKKE EETEEENVHV IDIPGLGVVP IPQGFDTETL MQLPEEYRIE AVMNYYQEHN IPRIRRSEER RGIQLSHNIR KFPCEEFKQR RINKLEMNSN TLKKTWISSS SKTVPEFNIK KALSIYFVCT EDERKIILQK INSLFCNSQI NFNVVYNNFM KYIVNDLFIE ILQSKVDEKM IRYWQLVNDV NQQKDSEISS EINEMIIDNL CMMVEANMKN SIILNAVVYL PQILEIEEIK INKESYILKL LSIFGRYYRN RSHSNQLHFE SKTQRLIDKC LKKNIQVLHE KELNQDYL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccta gaaaacaatg taatgaacct atgaataaaa ataacgatat atttaaccaa tatatttact taacattgaa aggaaaatca atggaaataa agaatgatac tgatgatatt ttattttatc aattatgtca agaaagtttt ataactaaaa gaacaaaagg gatgttggaa gggaattggg atattaaatg gaaggaagat tgtttaaata aaattagaaa ggttattaaa attattagtg accaagttaa agacacacca caagaacatg ataaaatttc aaacaaagaa gaaaaaatgg aagaagaaaa tagtgaaata aaagagttaa aagaacttgt acatattctt aagattaatg aagaaattga agatattgta aaaagattta tcaaatatag tgagaaaaat aagattgata aaatgaaaga aatgaaatca ttaatgatta tgtatcaaga aattctaaca gaacaagaga tgaaagatgg atgtaataca attattagtt tcatgaataa atttaaagga atgaatataa aaatcgacta tcaagaagat aaaaaggaag aagaaacaga agaagaaaat gttcatgtta ttgatattcc tgggttaggt gtagttccta ttcctcaagg atttgatact gaaacattaa tgcaattacc tgaagaatat agaatagaag cagtaatgaa ttattatcaa gaacataata ttccaagaat acgaagaagt gaagagagaa gaggtattca attatcacat aatataagaa aattcccgtg tgaagaattt aaacaaagaa ggataaataa attggaaatg aattcaaata cactcaaaaa aacatggata agttctagtt caaagactgt acctgaattt aacatcaaaa aagcattgag tatatatttt gtttgtacag aagatgaacg taaaattata ttacagaaga taaattcatt attctgtaat tcacaaatta acttcaatgt tgtatataat aattttatga aatatattgt caatgattta tttattgaaa ttcttcaaag taaagttgat gagaaaatga ttagatattg gcaattagtc aatgatgtta atcaacaaaa agattcagaa atatcttcag aaattaatga aatgataatt gataatttat gtatgatggt tgaagctaac atgaaaaatt caataattct caatgcagtt gtatatttac ctcaaatatt agaaatagag gaaattaaaa ttaataaaga aagttacata ttaaaattat tatctatctt tggaaggtat tatcgaaata gatcacattc taaccaactt cattttgaat caaaaacaca acgtcttatt gataaatgtt taaagaaaaa tatacaagtt ttacatgaaa aagaattaaa tcaagactat ttatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat