SSGCID: Target Page

EnhiA.18459.a

Ubiquitin carboxyl-terminal hydrolase domain containing protein

CENTER ID:	EnhiA.18459.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.18459.a.B3.GE35788	region2		740	937
EnhiA.18459.a.B4.GE35789	region3		938	1143

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_110170
RefSeq:	XP_657027.1
UniProt:	C4LU71

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTQRHPPVN FNDRAKLFIG GLSSNRIDLQ IQALRNIEIE FLEYEDDAAE KSLLYSFTKY EKKAPKSNYS HHVATKKELI NFLEHTSKII INLVVTCPHE ELLRHSIPII IRLYQNKLLP NNFLKKLYQN AERLSGTIVM RAQDLILRLF NKLSSEEKQY FKLLAINGSE IGQIESLHFL CKLLAIHEIP TEVFQKLIKP ICKNIQHLES DVFPLIQFPE LSVLLRKSLL TEYITNFQDI GVLSALKVIA ETAPFESDVD DIKQLLDMSI FNETENLKDV LINLAQKNEN LFPSCLLGMI RLEGFNETTK WLTEEMIISH IEDLMKVLLT YNELPETSYS IVINAFIQYN ILITKSIHTE SNGNIIFDNY NNLVGKEILW DVFLKKKNQN IENLLMKVYQ GKSKEYIIDA LHAFMKNPQL IAAEIIEKSL KDLKQSLEEN NKGMIKLKRI IKLKCNIPVG KIKVKNISYL YQSIDDIKQL KSFSEEINNK IIQTKQKLEP EIEIEEKEYL FETPIEVIIT DILANVYKQK GTSAEWIKRV IKNNKYLVTT QIDGKDVNIN DIIGNLVKKD GSIFLYLQEG VKKLNGQKLT NEKSINTIIQ CEPKTIKQLS FIIEENEIKN VIELIQKKEM YNIGIILLKE INPFIKWRLS AIENCKEQTG YEFIGFERLM AQNEINEDEF KKLISMFCHS IEIRKGNEKY LAEVLLHFEQ LIKKELWHIN EEEIKKIQKR NITEGNERII TTICDIILLR NNQTIEWNKE VVIEPIKRVV KELMNEELFE TLIPYLEEPS ELVEECYEII IQYIDKKYNT DDLFIQKMVG NVVNRGKGFK FSVRAMKLMK DKQKLLQPYL IALIDSLFPK GKLNEIKEDI LHDYIEMICE SCKSCNEEQK KEICHHFDMI LSMLESSGNH METRKGLWCG LTNQGCTCYM NSILQQLLHT KVFCNKLLEK RVILPKYIEN NEENKGQIDI NVINSEKVDN CKTKNNVDKV QLLKKENEAK YNITNNETIT KEIIQGIEQS DNIISKTPEE IEQNINSQKE NIQNKEQNEF ITHNNNELKD YRMIEGLQKL FVEMKESKLQ TVDTKEFCKE AHGSCYEEVN VYEQMDAQEF FNTIISTLEV CKKEKNIIEA RKVFTGETEI SIKSQCGHIV KRNEEFCSCA LEVEHYQTMG QSLKALCEGC ILCDYRCEQC GLKSDSQRSE RYKKLPETLV FHLKRFGYNE QFQIVKYNGK FEYPNEIDLK QYCCTEYKGI TKYKLVGIVV HTGNAHGGHY FSNILLNDQW ISFNDKLIEN IGNGNWFGGE VSNAYMLFYH QINTLEENEN ETIMIDDLLL KRVEKINYSY ILSKQASEPF VIDFIKGIIE SCKYIGNTLS FLRRIILKKN PEQCLGVLKE ISQIMDKEMA REVLQKMSTY YLKDYLMWGK SMIVSAIKQI QEPVFNELII KEVENGNSYG IEVIEELYKL NQVRLSTCVR LSVHIIKNQK YNYVQGEIAC SFILKGIQQK IYQFDDDVLL SFKSTNAPEN LIQEIIKNYP TQKVNGCALK LIKSALGKRE VTDEEMIKLR RLVCNESCAT NIVKECLKHL GMAPEIYRYF KVILITMQII LLSPLIYNEL KKKIKEFNEN LDFYSLKIVL IE

NT Sequence

atgtcaacgc aacgacatcc tccagtaaat tttaatgata gagcaaagtt gtttattggt ggtctcagta gtaatagaat tgatttacaa attcaagcac taaggaatat agaaattgaa tttcttgaat atgaagatga tgcagcagaa aagtcattgc tttattcatt tacaaaatat gaaaagaagg cacctaaatc taattattca catcatgtcg caacaaaaaa agagttaata aattttctag aacatacgtc aaagattatt attaatttag ttgttacttg tcctcatgaa gaattactac gtcattcaat tcctattata attagactat atcaaaataa gttattacca aataactttt taaaaaaatt atatcaaaat gcagaaaggt tgtctgggac aattgttatg agagcacaag atttaatcct gagattattt aataaattat cttcggaaga aaaacaatat tttaaattat tagccattaa tgggtctgaa attggtcaaa ttgaatctct tcattttctt tgtaaactat tagcaataca cgaaattcct actgaggttt ttcaaaaatt aataaaacca atttgtaaga acattcaaca cttagaaagt gatgtttttc ctttaataca atttcctgaa ctaagcgtgt tattaaggaa aagtctatta acagaatata taacaaattt tcaagacata ggagttttaa gtgcacttaa ggttattgca gagactgctc cattcgaaag tgatgtagat gatattaaac agttactcga tatgtctatt tttaatgaaa ccgaaaattt aaaagatgta ttgattaact tagcacagaa aaatgaaaat ttatttcctt cttgtttact aggaatgata agacttgagg gattcaacga aacaacaaaa tggttaacag aagaaatgat tattagtcat attgaagatt taatgaaagt tcttctgaca tacaacgaat tacctgaaac atcatattca attgtaataa atgcctttat tcaatacaac attttaataa caaaaagtat tcacaccgaa tctaatggta atattatatt cgataattat aataatttag ttggaaaaga aatactctgg gatgtatttt taaaaaagaa aaatcaaaac attgaaaatc ttttaatgaa agtatatcaa ggaaaatcaa aggaatatat cattgatgct ttacatgcct ttatgaaaaa tccacaactc attgctgctg aaataattga aaaatcatta aaagatttaa aacaatcact ggaagaaaat aacaagggaa tgattaaatt aaaacgaatt attaaattaa aatgcaatat tcccgttgga aaaattaaag ttaaaaacat atcatatttg tatcaatcca ttgatgatat caaacaatta aaatcttttt cagaagaaat taataataaa atcatacaaa ctaaacaaaa acttgaacca gaaattgaaa ttgaagaaaa agagtattta tttgaaacac caattgaagt tataatcact gatatattag caaatgtgta taaacaaaaa ggtacttcag cagaatggat aaaaagagtt attaaaaaca ataagtattt ggttaccact caaattgatg gtaaagacgt taatataaat gatattattg gaaacttagt taagaaagat gggagtattt ttttgtatct tcaggaagga gttaaaaaat taaatggtca aaaattaaca aatgaaaaaa gtattaatac aataattcaa tgtgaaccaa aaacaataaa acaactatct tttataattg aagaaaatga aataaaaaat gttattgaac ttattcaaaa aaaagaaatg tataacattg gaattatact tctaaaagaa attaatccat ttattaaatg gagactatca gcaattgaaa actgtaaaga acaaactggt tatgaattta ttggattcga acgattaatg gcacagaatg aaataaatga ggatgaattt aaaaaactta tttcaatgtt ttgtcattct attgaaataa gaaaaggtaa tgagaaatat cttgctgaag ttctcttaca ttttgaacaa ctaatcaaaa aagaattatg gcatataaat gaagaagaaa tcaaaaaaat tcaaaaaaga aatattacag aaggaaatga aagaattatt actacaatat gtgatatcat attattaaga aataatcaaa caattgaatg gaataaagaa gtggtcatag aaccaataaa aagagtggtt aaagaattga tgaacgaaga attatttgaa acgttaattc cttatttaga agagccaagt gaattagttg aagaatgtta tgaaataatt attcaatata ttgataaaaa atataatact gatgatttgt ttattcagaa aatggttgga aatgttgtta atagaggaaa agggtttaaa tttagtgttc gagcaatgaa attaatgaaa gacaaacaaa aacttcttca accttatctt attgcactaa ttgattcatt atttccaaaa ggaaagttaa atgaaatcaa agaagatata ctccatgatt atattgaaat gatatgtgag tcttgtaaga gctgtaatga agaacaaaag aaggaaattt gtcatcattt tgatatgata ttaagtatgt tagagagtag tggaaatcat atggaaacaa gaaaaggatt gtggtgtggc ttaactaatc aaggatgtac ttgttatatg aatagtattc ttcaacaatt attacatact aaagtgtttt gtaataaact tcttgaaaaa agagttattt tacctaaata tattgaaaat aatgaagaaa ataaaggtca aattgatata aatgttataa atagtgaaaa agttgataat tgtaaaacaa agaataatgt tgacaaggtc caactattaa aaaaagaaaa tgaagctaaa tataatatta ctaacaatga aactatcaca aaagaaataa ttcaagggat agaacaaagt gataatataa taagtaaaac acctgaagaa attgaacaaa acattaattc acaaaaagag aatattcaaa ataaagaaca aaatgaattt atcacgcata acaataatga acttaaagac tatagaatga tagaaggatt acaaaaatta tttgttgaaa tgaaagaaag taaattacaa actgtcgaca caaaagagtt ttgtaaagaa gcacatggaa gttgctatga agaagttaat gtctatgaac aaatggatgc tcaagaattt tttaatacaa taatatcaac attagaagtg tgtaaaaaag agaaaaatat cattgaagca agaaaagtat ttactggaga aacagaaata tcaatcaaaa gtcaatgtgg tcatatagta aaacgaaatg aagaattttg ttcatgtgca ttagaagtag aacattacca aacaatggga caatcattaa aagcactatg tgaaggttgt attttatgtg attatcgttg tgaacaatgt ggattaaaaa gtgattctca aagaagtgaa agatataaaa aattaccaga aacattagta tttcatttga aacgatttgg atataatgaa caatttcaaa ttgtcaaata taatggaaaa tttgaatatc caaatgaaat tgatttgaaa caatattgtt gtacagagta taaaggaata actaaatata aattagttgg aatagttgtt catactggaa atgcacatgg tggtcattat tttagtaata tactacttaa tgaccaatgg atatctttta atgataaatt aattgaaaac ataggtaacg gaaattggtt tggaggagaa gtgtcaaatg cttatatgtt attttatcac caaataaata cattagaaga aaatgaaaat gaaacaatta tgattgatga cttattacta aaaagagtag aaaaaataaa ttatagctat attttatcaa aacaagcatc agaacctttt gttattgatt ttattaaagg aattattgaa agttgtaaat acattggaaa tacattatca ttcttacgta gaattattct taaaaaaaat cctgaacaat gtcttggagt tcttaaagag atttctcaaa taatggacaa agaaatggct agagaggtat tacaaaaaat gtcaacatat tatttgaaag attatttaat gtgggggaag tctatgattg tctcagcaat taaacaaatc caagaacctg tttttaatga gttaataata aaagaagtag agaatggaaa tagttatgga atcgaggtta ttgaagaatt atataaatta aatcaagtta ggttgagtac ttgtgtaaga ctttcagttc atattattaa aaaccagaaa tataattatg tacaaggtga aattgcatgt tcttttatac tcaaaggaat tcaacaaaaa atttatcaat ttgatgatga tgtgttatta agttttaaaa gtacaaatgc tccagaaaat ctcatacaag aaataattaa gaattatcca actcaaaaag ttaatggttg tgcattaaaa ctaataaaaa gtgcattagg taaacgagaa gttactgatg aagaaatgat aaaattaaga agattagtat gtaatgaaag ttgtgcaact aatattgtaa aagagtgttt aaaacatctt ggtatggcac cagaaatata taggtacttt aaagtaattc taataacaat gcaaattata ttactttctc ctttaattta taatgaatta aagaaaaaga taaaagaatt taatgaaaat ttagactttt actcacttaa aatagtttta attgaataa

Details for EnhiA.18459.a.B3.GE35788

HARVESTED ON:	7/31/2012
SEQUENCED ON:	8/3/2012
EXPECTED MW:	24kDa
OBSERVED MW:	20kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMI TTICDIILLR NNQTIEWNKE VVIEPIKRVV KELMNEELFE TLIPYLEEPS ELVEECYEII IQYIDKKYNT DDLFIQKMVG NVVNRGKGFK FSVRAMKLMK DKQKLLQPYL IALIDSLFPK GKLNEIKEDI LHDYIEMICE SCKSCNEEQK KEICHHFDMI LSMLESSGNH METRKGLWCG LTNQGCTCYM NSILQQL

Validated NT Sequence

attttgttta actttaagaa ggagatatac catggctcac caccaccacc accatatgat tactacaata tgtgatatca tattattaag aaataatcaa acaattgaat ggaataaaga agtggtcata gaaccaataa aaagagtggt taaagaattg atgaacgaag aattatttga aacgttaatt ccttatttag aagagccaag tgaattagtt gaagaatgtt atgaaataat tattcaatat attgataaaa aatataatac tgatgatttg tttattcaga aaatggttgg aaatgttgtt aatagaggaa aagggtttaa atttagtgtt cgagcaatga aattaatgaa agacaaacaa aaacttcttc aaccttatct tattgcacta attgattcat tatttccaaa aggaaagtta aatgaaatca aagaagatat actccatgat tatattgaaa tgatatgtga gtcttgtaag agctgtaatg aagaacaaaa gaaggaaatt tgtcatcatt ttgatatgat attaagtatg ttagagagta gtggaaatca tatggaaaca agaaaaggat tgtggtgtgg cttaactaat caaggatgta cttgttatat gaatagtatt cttcaacaat tataagtgag taagatagga tccggctgct aacaagcccc cganaga

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatta ctacaatatg tgatatcata ttattaagaa ataatcaaac aattgaatgg aataaagaag tggtcataga accaataaaa agagtggtta aagaattgat gaacgaagaa ttatttgaaa cgttaattcc ttatttagaa gagccaagtg aattagttga agaatgttat gaaataatta ttcaatatat tgataaaaaa tataatactg atgatttgtt tattcagaaa atggttggaa atgttgttaa tagaggaaaa gggtttaaat ttagtgttcg agcaatgaaa ttaatgaaag acaaacaaaa acttcttcaa ccttatctta ttgcactaat tgattcatta tttccaaaag gaaagttaaa tgaaatcaaa gaagatatac tccatgatta tattgaaatg atatgtgagt cttgtaagag ctgtaatgaa gaacaaaaga aggaaatttg tcatcatttt gatatgatat taagtatgtt agagagtagt ggaaatcata tggaaacaag aaaaggattg tggtgtggct taactaatca aggatgtact tgttatatga atagtattct tcaacaatta tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for EnhiA.18459.a.B4.GE35789

HARVESTED ON:	data unavailable
SEQUENCED ON:	8/3/2012
EXPECTED MW:	25kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML HTKVFCNKLL EKRVILPKYI ENNEENKGQI DINVINSEKV DNCKTKNNVD KVQLLKKENE AKYNITNNET ITKEIIQGIE QSDNIISKTP EEIEQNINSQ KENIQNKEQN EFITHNNNEL KDYRMIEGLQ KLFVEMKESK LQTVDTKEFC KEAHGSCYEE VNVYEQMDAQ EFFNTIISTL EVCKKEKNII EARKVFTGET EISIK

Validated NT Sequence

ttgtttanct ttaagaagga ganataccat ggctcaccac caccaccacc atatgttaca tactaaagtg ttttgtaata aacttcttga aaaaagagtt attttaccta aatatattga aaataatgaa gaaaataaag gtcaaattga tataaatgtt ataaatagtg aaaaagttga taattgtaaa acaaagaata atgttgacaa ggtccaacta ttaaaaaaag aaaatgaagc taaatataat attactaaca atgaaactat cacaaaagaa ataattcaag ggatagaaca aagtgataat ataataagta aaacacctga agaaattgaa caaaacatta attcacaaaa agagaatatt caaaataaag aacaaaatga atttatcacg cataacaata atgaacttaa agactataga atgatagaag gattacaaaa attatttgtt gaaatgaaag aaagtaaatt acaaactgtc gacacaaaag agttttgtaa agaagcacat ggaagttgct atgaagaagt taatgtctat gaacaaatgg atgctcaaga attttttaat acaataatat caacattaga agtgtgtaaa aaagagaaaa atatcattga agcaagaaaa gtatttactg gagaaacaga aatatcaatc aaataagtga gtaagatagg atccggctgc taacagcccc cnnaang

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttac atactaaagt gttttgtaat aaacttcttg aaaaaagagt tattttacct aaatatattg aaaataatga agaaaataaa ggtcaaattg atataaatgt tataaatagt gaaaaagttg ataattgtaa aacaaagaat aatgttgaca aggtccaact attaaaaaaa gaaaatgaag ctaaatataa tattactaac aatgaaacta tcacaaaaga aataattcaa gggatagaac aaagtgataa tataataagt aaaacacctg aagaaattga acaaaacatt aattcacaaa aagagaatat tcaaaataaa gaacaaaatg aatttatcac gcataacaat aatgaactta aagactatag aatgatagaa ggattacaaa aattatttgt tgaaatgaaa gaaagtaaat tacaaactgt cgacacaaaa gagttttgta aagaagcaca tggaagttgc tatgaagaag ttaatgtcta tgaacaaatg gatgctcaag aattttttaa tacaataata tcaacattag aagtgtgtaa aaaagagaaa aatatcattg aagcaagaaa agtatttact ggagaaacag aaatatcaat caaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat