EnhiA.18453.a

Putative uncharacterized protein

CENTER ID: EnhiA.18453.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.18453.a.B1.GE35786 full length 1 843
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.18453.a.B1.PS01674 full length 1 843

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_101240
RefSeq: XP_657012.1
UniProt: C4M8B5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPAQANKKGV SKKGDASDKK VFMNAIVTGI TEKNGLLLAT KKEEEKNPVI VIAYLELPRY DKKQNKEETF AYECGELLRK KLINGKEVKF EVVSEATENV PKMVIPFVNG ENIAITLLEN GYAVLKGKGK EIAEYIKAEE KAKKEKKGVH NENAEKISIK KPIDPKLTSK ELDFYVMKNL NGHIKKVDAP GQFIIETPER KIVRVSLFGI NQHATFNVEK KQFEFDDIAK EAIKFNNKNY NQRDCVVKVM DNGKRVTGII TVDKKDIAEE LLKNGYVTIS TFEENMESVK QVYEECEAVA KKERKGRFVN FDQAAEDAAK AKKEKREKDL KERKKNARVI RGGNILFIGK YLRIEKDGEV LNVNFASIKP LYKKDDKAFN ELIEFRMREV IRKLIVGKKF ECKEAYKSEN KKGEETVTEV YYDVYADKTN IAIPLLKEGL VVVDERTKDQ FYQSFDYSKL KAVPKKTYKP VPITNYSNLD NVKDMLIGNN FKCVIEKAIS LTVYLVYIPE KNSQLIVHLQ NCFVPKDDSN DSLKKFVESA KTNVKQLCEY REVVVDFKRY HKGNLYVEII SNGTDLASYI LEHGFAIPTG RYNVSSEIEQ MKENMTQIYQ FDTQKKKEVT EVIEKKPKRN TEIKFSAEQK VYLTGFDGEK IGYYDNAEDA KIIEEVNAMC KTVKKGAIKP AVGDKCIVEN KGFFYRAEFV EVATGANVLK CIDTGAVIIC GKNKLKPITE EIAGKPVTVK TVGLVGLKTL TPKDVDFAAA VEAVAAYYKK EGSLFVSGEE AKVVVGDVCI NNHLVQLGLA FVDKHFHDNS EWGVDLVAAQ LQAKENHLNV WRYGEVADEE EKN
NT Sequence
atgccagcac aagctaataa gaagggagtc tcaaagaaag gtgatgcctc tgataagaaa gtattcatga acgctattgt tacaggaatt actgagaaga atggacttct tcttgcaaca aagaaagaag aagaaaagaa tccagttatt gtgattgcat atcttgaact tccaagatat gataaaaaac aaaataaaga agagacattt gcttatgaat gtggggaatt attaagaaaa aaacttatta atggtaaaga agttaaattt gaagttgtta gtgaagcaac tgagaatgtt ccaaagatgg taattccatt tgttaatgga gaaaatattg caattactct tcttgaaaat ggatatgctg ttttaaaggg aaaaggaaaa gaaattgcag aatatattaa agctgaagaa aaggctaaaa aagaaaagaa aggagttcat aatgagaacg cagaaaaaat ttctataaaa aaaccaattg atccaaaatt aacaagtaaa gaacttgact tttatgtaat gaaaaatctt aatggacata ttaagaaagt tgatgcacca ggacaattca ttattgaaac tccagaaaga aaaattgtaa gagtttcact ctttggaatt aatcaacatg caacatttaa tgtagaaaag aaacaatttg aatttgatga tattgctaaa gaagctataa aattcaacaa taagaattat aatcaaagag attgtgttgt taaagttatg gacaatggaa agagagtaac aggaattatt acagttgata agaaagatat tgctgaagaa ttattaaaga atggatatgt cactatttct acatttgaag aaaatatgga atcagtaaag caagtatatg aagaatgtga agcagttgct aaaaaagaaa gaaaaggaag atttgttaat tttgatcaag cggctgaaga tgcagccaaa gctaaaaaag aaaagagaga aaaagacctt aaagaaagaa agaagaatgc tagagtaatt agaggaggta atattctttt cattggtaaa tatcttagaa ttgaaaaaga tggagaagtt ctcaatgtta actttgcatc aattaaacca ctttataaga aagacgacaa agcatttaat gaacttattg aatttagaat gagagaggtt attagaaaat taattgttgg aaagaaattt gaatgtaaag aagcttataa atcagaaaat aaaaaaggag aagaaactgt tactgaagtt tattatgatg tttatgcaga taaaacaaat attgctattc cattattaaa agaaggactt gttgtagttg atgaaagaac taaggaccaa ttctatcaat catttgatta tagtaaatta aaagctgttc caaagaaaac atataaacca gttccaatta ctaattattc taatttggat aacgttaaag atatgcttat tggaaataat ttcaaatgtg ttattgaaaa ggctatttca ttaaccgtat atcttgttta cattccagag aaaaattctc aacttattgt tcatcttcaa aattgttttg ttccaaaaga tgattctaat gatagtctta agaaatttgt tgagagtgct aaaacaaatg ttaaacaatt atgtgaatat cgtgaagttg ttgtagactt taagagatat cataaaggaa acttatatgt tgaaattatt agtaatggaa cagatttagc tagttatatt cttgaacatg gatttgctat tccaactgga cgttacaatg tttcatctga aattgaacaa atgaaagaaa atatgactca aatttatcaa tttgatactc aaaagaagaa agaagtcact gaagttattg aaaagaaacc aaagagaaat actgaaatta aattcagtgc tgaacaaaaa gtttatttaa caggttttga tggagaaaag attggttatt atgataatgc tgaagatgct aaaattattg aagaagttaa tgctatgtgt aagactgtta agaaaggtgc tataaaacca gctgttggtg ataaatgtat tgttgagaat aaaggatttt tctatagagc tgaatttgtt gaagttgcaa ctggtgctaa tgttcttaaa tgcattgata caggagctgt tattatttgt ggtaaaaaca aattaaaacc aatcacagaa gaaattgctg gtaaacctgt tactgttaaa actgtaggac ttgtaggact taagactctc actcctaaag atgttgattt cgctgctgct gttgaagctg ttgctgctta ttataagaaa gaaggttcac tctttgtttc tggagaagaa gctaaggttg ttgttggaga tgtttgcatt aacaatcacc ttgtccaact tggtcttgca tttgttgaca aacacttcca tgacaatagt gaatggggtg ttgatttagt agctgctcaa ttacaagcta aagaaaacca tctcaacgtt tggagatatg gtgaagttgc tgatgaagaa gaaaagaact aa
Details for EnhiA.18453.a.B1.GE35786
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/6/2012
EXPECTED MW: 96kDa
OBSERVED MW: 100kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 8
Validated AA Sequence
MKNLNGHIKK VDAPGQFIIE TPERKIVRVS LFGINQHATF NVEKKQFEFD DIAKEAIKFN NKNYNXRDXV VKVMDN
Validated NT Sequence
aaaagaaagg agttcataat gagaacgcag aaaaaatttc tataaaaaaa ccaattgatc caaaattaac aagtaaagaa cttgactttt atgtaatgaa aaatcttaat ggacatatta agaaagttga tgcaccagga caattcatta ttgaaactcc agaaagaaaa attgtaagag tttcactctt tggaattaat caacatgcaa catttaatgt agaaaagaaa caatttgaat ttgatgatat tgctaaagaa gctataaaat tcaacaataa gaattataat cnaagagatt gngttgttaa agttatggac aatgg
Expected Protein Sequence
MAHHHHHHMP AQANKKGVSK KGDASDKKVF MNAIVTGITE KNGLLLATKK EEEKNPVIVI AYLELPRYDK KQNKEETFAY ECGELLRKKL INGKEVKFEV VSEATENVPK MVIPFVNGEN IAITLLENGY AVLKGKGKEI AEYIKAEEKA KKEKKGVHNE NAEKISIKKP IDPKLTSKEL DFYVMKNLNG HIKKVDAPGQ FIIETPERKI VRVSLFGINQ HATFNVEKKQ FEFDDIAKEA IKFNNKNYNQ RDCVVKVMDN GKRVTGIITV DKKDIAEELL KNGYVTISTF EENMESVKQV YEECEAVAKK ERKGRFVNFD QAAEDAAKAK KEKREKDLKE RKKNARVIRG GNILFIGKYL RIEKDGEVLN VNFASIKPLY KKDDKAFNEL IEFRMREVIR KLIVGKKFEC KEAYKSENKK GEETVTEVYY DVYADKTNIA IPLLKEGLVV VDERTKDQFY QSFDYSKLKA VPKKTYKPVP ITNYSNLDNV KDMLIGNNFK CVIEKAISLT VYLVYIPEKN SQLIVHLQNC FVPKDDSNDS LKKFVESAKT NVKQLCEYRE VVVDFKRYHK GNLYVEIISN GTDLASYILE HGFAIPTGRY NVSSEIEQMK ENMTQIYQFD TQKKKEVTEV IEKKPKRNTE IKFSAEQKVY LTGFDGEKIG YYDNAEDAKI IEEVNAMCKT VKKGAIKPAV GDKCIVENKG FFYRAEFVEV ATGANVLKCI DTGAVIICGK NKLKPITEEI AGKPVTVKTV GLVGLKTLTP KDVDFAAAVE AVAAYYKKEG SLFVSGEEAK VVVGDVCINN HLVQLGLAFV DKHFHDNSEW GVDLVAAQLQ AKENHLNVWR YGEVADEEEK N
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccag cacaagctaa taagaaggga gtctcaaaga aaggtgatgc ctctgataag aaagtattca tgaacgctat tgttacagga attactgaga agaatggact tcttcttgca acaaagaaag aagaagaaaa gaatccagtt attgtgattg catatcttga acttccaaga tatgataaaa aacaaaataa agaagagaca tttgcttatg aatgtgggga attattaaga aaaaaactta ttaatggtaa agaagttaaa tttgaagttg ttagtgaagc aactgagaat gttccaaaga tggtaattcc atttgttaat ggagaaaata ttgcaattac tcttcttgaa aatggatatg ctgttttaaa gggaaaagga aaagaaattg cagaatatat taaagctgaa gaaaaggcta aaaaagaaaa gaaaggagtt cataatgaga acgcagaaaa aatttctata aaaaaaccaa ttgatccaaa attaacaagt aaagaacttg acttttatgt aatgaaaaat cttaatggac atattaagaa agttgatgca ccaggacaat tcattattga aactccagaa agaaaaattg taagagtttc actctttgga attaatcaac atgcaacatt taatgtagaa aagaaacaat ttgaatttga tgatattgct aaagaagcta taaaattcaa caataagaat tataatcaaa gagattgtgt tgttaaagtt atggacaatg gaaagagagt aacaggaatt attacagttg ataagaaaga tattgctgaa gaattattaa agaatggata tgtcactatt tctacatttg aagaaaatat ggaatcagta aagcaagtat atgaagaatg tgaagcagtt gctaaaaaag aaagaaaagg aagatttgtt aattttgatc aagcggctga agatgcagcc aaagctaaaa aagaaaagag agaaaaagac cttaaagaaa gaaagaagaa tgctagagta attagaggag gtaatattct tttcattggt aaatatctta gaattgaaaa agatggagaa gttctcaatg ttaactttgc atcaattaaa ccactttata agaaagacga caaagcattt aatgaactta ttgaatttag aatgagagag gttattagaa aattaattgt tggaaagaaa tttgaatgta aagaagctta taaatcagaa aataaaaaag gagaagaaac tgttactgaa gtttattatg atgtttatgc agataaaaca aatattgcta ttccattatt aaaagaagga cttgttgtag ttgatgaaag aactaaggac caattctatc aatcatttga ttatagtaaa ttaaaagctg ttccaaagaa aacatataaa ccagttccaa ttactaatta ttctaatttg gataacgtta aagatatgct tattggaaat aatttcaaat gtgttattga aaaggctatt tcattaaccg tatatcttgt ttacattcca gagaaaaatt ctcaacttat tgttcatctt caaaattgtt ttgttccaaa agatgattct aatgatagtc ttaagaaatt tgttgagagt gctaaaacaa atgttaaaca attatgtgaa tatcgtgaag ttgttgtaga ctttaagaga tatcataaag gaaacttata tgttgaaatt attagtaatg gaacagattt agctagttat attcttgaac atggatttgc tattccaact ggacgttaca atgtttcatc tgaaattgaa caaatgaaag aaaatatgac tcaaatttat caatttgata ctcaaaagaa gaaagaagtc actgaagtta ttgaaaagaa accaaagaga aatactgaaa ttaaattcag tgctgaacaa aaagtttatt taacaggttt tgatggagaa aagattggtt attatgataa tgctgaagat gctaaaatta ttgaagaagt taatgctatg tgtaagactg ttaagaaagg tgctataaaa ccagctgttg gtgataaatg tattgttgag aataaaggat ttttctatag agctgaattt gttgaagttg caactggtgc taatgttctt aaatgcattg atacaggagc tgttattatt tgtggtaaaa acaaattaaa accaatcaca gaagaaattg ctggtaaacc tgttactgtt aaaactgtag gacttgtagg acttaagact ctcactccta aagatgttga tttcgctgct gctgttgaag ctgttgctgc ttattataag aaagaaggtt cactctttgt ttctggagaa gaagctaagg ttgttgttgg agatgtttgc attaacaatc accttgtcca acttggtctt gcatttgttg acaaacactt ccatgacaat agtgaatggg gtgttgattt agtagctgct caattacaag ctaaagaaaa ccatctcaac gtttggagat atggtgaagt tgctgatgaa gaagaaaaga actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for EnhiA.18453.a.B1.PS01674
PURIFICATION DATe: 11/13/2012
CONCENTRATION: 46.8mg/ml
OBSERVED MW: 90kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 8
Protocol Notes
notes unavailable
Validated AA Sequence
MKNLNGHIKK VDAPGQFIIE TPERKIVRVS LFGINQHATF NVEKKQFEFD DIAKEAIKFN NKNYNXRDXV VKVMDN
Validated NT Sequence
aaaagaaagg agttcataat gagaacgcag aaaaaatttc tataaaaaaa ccaattgatc caaaattaac aagtaaagaa cttgactttt atgtaatgaa aaatcttaat ggacatatta agaaagttga tgcaccagga caattcatta ttgaaactcc agaaagaaaa attgtaagag tttcactctt tggaattaat caacatgcaa catttaatgt agaaaagaaa caatttgaat ttgatgatat tgctaaagaa gctataaaat tcaacaataa gaattataat cnaagagatt gngttgttaa agttatggac aatgg
Expressed Protein Sequence
MAHHHHHHMP AQANKKGVSK KGDASDKKVF MNAIVTGITE KNGLLLATKK EEEKNPVIVI AYLELPRYDK KQNKEETFAY ECGELLRKKL INGKEVKFEV VSEATENVPK MVIPFVNGEN IAITLLENGY AVLKGKGKEI AEYIKAEEKA KKEKKGVHNE NAEKISIKKP IDPKLTSKEL DFYVMKNLNG HIKKVDAPGQ FIIETPERKI VRVSLFGINQ HATFNVEKKQ FEFDDIAKEA IKFNNKNYNQ RDCVVKVMDN GKRVTGIITV DKKDIAEELL KNGYVTISTF EENMESVKQV YEECEAVAKK ERKGRFVNFD QAAEDAAKAK KEKREKDLKE RKKNARVIRG GNILFIGKYL RIEKDGEVLN VNFASIKPLY KKDDKAFNEL IEFRMREVIR KLIVGKKFEC KEAYKSENKK GEETVTEVYY DVYADKTNIA IPLLKEGLVV VDERTKDQFY QSFDYSKLKA VPKKTYKPVP ITNYSNLDNV KDMLIGNNFK CVIEKAISLT VYLVYIPEKN SQLIVHLQNC FVPKDDSNDS LKKFVESAKT NVKQLCEYRE VVVDFKRYHK GNLYVEIISN GTDLASYILE HGFAIPTGRY NVSSEIEQMK ENMTQIYQFD TQKKKEVTEV IEKKPKRNTE IKFSAEQKVY LTGFDGEKIG YYDNAEDAKI IEEVNAMCKT VKKGAIKPAV GDKCIVENKG FFYRAEFVEV ATGANVLKCI DTGAVIICGK NKLKPITEEI AGKPVTVKTV GLVGLKTLTP KDVDFAAAVE AVAAYYKKEG SLFVSGEEAK VVVGDVCINN HLVQLGLAFV DKHFHDNSEW GVDLVAAQLQ AKENHLNVWR YGEVADEEEK N
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCAG CACAAGCTAA TAAGAAGGGA GTCTCAAAGA AAGGTGATGC CTCTGATAAG AAAGTATTCA TGAACGCTAT TGTTACAGGA ATTACTGAGA AGAATGGACT TCTTCTTGCA ACAAAGAAAG AAGAAGAAAA GAATCCAGTT ATTGTGATTG CATATCTTGA ACTTCCAAGA TATGATAAAA AACAAAATAA AGAAGAGACA TTTGCTTATG AATGTGGGGA ATTATTAAGA AAAAAACTTA TTAATGGTAA AGAAGTTAAA TTTGAAGTTG TTAGTGAAGC AACTGAGAAT GTTCCAAAGA TGGTAATTCC ATTTGTTAAT GGAGAAAATA TTGCAATTAC TCTTCTTGAA AATGGATATG CTGTTTTAAA GGGAAAAGGA AAAGAAATTG CAGAATATAT TAAAGCTGAA GAAAAGGCTA AAAAAGAAAA GAAAGGAGTT CATAATGAGA ACGCAGAAAA AATTTCTATA AAAAAACCAA TTGATCCAAA ATTAACAAGT AAAGAACTTG ACTTTTATGT AATGAAAAAT CTTAATGGAC ATATTAAGAA AGTTGATGCA CCAGGACAAT TCATTATTGA AACTCCAGAA AGAAAAATTG TAAGAGTTTC ACTCTTTGGA ATTAATCAAC ATGCAACATT TAATGTAGAA AAGAAACAAT TTGAATTTGA TGATATTGCT AAAGAAGCTA TAAAATTCAA CAATAAGAAT TATAATCAAA GAGATTGTGT TGTTAAAGTT ATGGACAATG GAAAGAGAGT AACAGGAATT ATTACAGTTG ATAAGAAAGA TATTGCTGAA GAATTATTAA AGAATGGATA TGTCACTATT TCTACATTTG AAGAAAATAT GGAATCAGTA AAGCAAGTAT ATGAAGAATG TGAAGCAGTT GCTAAAAAAG AAAGAAAAGG AAGATTTGTT AATTTTGATC AAGCGGCTGA AGATGCAGCC AAAGCTAAAA AAGAAAAGAG AGAAAAAGAC CTTAAAGAAA GAAAGAAGAA TGCTAGAGTA ATTAGAGGAG GTAATATTCT TTTCATTGGT AAATATCTTA GAATTGAAAA AGATGGAGAA GTTCTCAATG TTAACTTTGC ATCAATTAAA CCACTTTATA AGAAAGACGA CAAAGCATTT AATGAACTTA TTGAATTTAG AATGAGAGAG GTTATTAGAA AATTAATTGT TGGAAAGAAA TTTGAATGTA AAGAAGCTTA TAAATCAGAA AATAAAAAAG GAGAAGAAAC TGTTACTGAA GTTTATTATG ATGTTTATGC AGATAAAACA AATATTGCTA TTCCATTATT AAAAGAAGGA CTTGTTGTAG TTGATGAAAG AACTAAGGAC CAATTCTATC AATCATTTGA TTATAGTAAA TTAAAAGCTG TTCCAAAGAA AACATATAAA CCAGTTCCAA TTACTAATTA TTCTAATTTG GATAACGTTA AAGATATGCT TATTGGAAAT AATTTCAAAT GTGTTATTGA AAAGGCTATT TCATTAACCG TATATCTTGT TTACATTCCA GAGAAAAATT CTCAACTTAT TGTTCATCTT CAAAATTGTT TTGTTCCAAA AGATGATTCT AATGATAGTC TTAAGAAATT TGTTGAGAGT GCTAAAACAA ATGTTAAACA ATTATGTGAA TATCGTGAAG TTGTTGTAGA CTTTAAGAGA TATCATAAAG GAAACTTATA TGTTGAAATT ATTAGTAATG GAACAGATTT AGCTAGTTAT ATTCTTGAAC ATGGATTTGC TATTCCAACT GGACGTTACA ATGTTTCATC TGAAATTGAA CAAATGAAAG AAAATATGAC TCAAATTTAT CAATTTGATA CTCAAAAGAA GAAAGAAGTC ACTGAAGTTA TTGAAAAGAA ACCAAAGAGA AATACTGAAA TTAAATTCAG TGCTGAACAA AAAGTTTATT TAACAGGTTT TGATGGAGAA AAGATTGGTT ATTATGATAA TGCTGAAGAT GCTAAAATTA TTGAAGAAGT TAATGCTATG TGTAAGACTG TTAAGAAAGG TGCTATAAAA CCAGCTGTTG GTGATAAATG TATTGTTGAG AATAAAGGAT TTTTCTATAG AGCTGAATTT GTTGAAGTTG CAACTGGTGC TAATGTTCTT AAATGCATTG ATACAGGAGC TGTTATTATT TGTGGTAAAA ACAAATTAAA ACCAATCACA GAAGAAATTG CTGGTAAACC TGTTACTGTT AAAACTGTAG GACTTGTAGG ACTTAAGACT CTCACTCCTA AAGATGTTGA TTTCGCTGCT GCTGTTGAAG CTGTTGCTGC TTATTATAAG AAAGAAGGTT CACTCTTTGT TTCTGGAGAA GAAGCTAAGG TTGTTGTTGG AGATGTTTGC ATTAACAATC ACCTTGTCCA ACTTGGTCTT GCATTTGTTG ACAAACACTT CCATGACAAT AGTGAATGGG GTGTTGATTT AGTAGCTGCT CAATTACAAG CTAAAGAAAA CCATCTCAAC GTTTGGAGAT ATGGTGAAGT TGCTGATGAA GAAGAAAAGA ACTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT