EnhiA.18437.a

Putative uncharacterized protein

CENTER ID: EnhiA.18437.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.18437.a.B5.GE35781 region4 1611 1935
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_114220
RefSeq: XP_651772.1
UniProt: C4M429

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKNKEQVVID DIFEKNDFVK SLKLVSSPRL VTPRIKTSKQ IKDQEQQRVL NIQFDEFDSE ETEQLDIFQL RIFLESGQFP ISSNQHLFVK EQNLREVLGS MFLDKSSRIC RKEFAKFCNY FEQKRDEIEW NSVSLISLFS QADNSNVPNK FKTYPTNHIT RFDLIHKQRL NKENIVLGGS VMDDYHTLIM NQFYQQTTTI QLDDNIEYIT TEEKIRKNSL VLEPGKYNRR EEMPCLFGTL FRDLNPSDLI KNEINDDVIK NKNIIYVIDL NNVVWAVGSI DNLILNFAIY DQSKKEKVSS NFIISPKQAD VNLIMNNNVG ETCAISLNES QIKNGKLWGV LTIYKLFDGE SMEEIFIAEK ESRNTLKIIE KMRQINVTQK AKGVQFLQYI ATGAMALNND LLEDRKSYYN LKLYYDDSII DLWQFLGNVE SGKIKPINGS WGFKFEQLNH YTKANLIITQ DGVVQNVDSQ PPEASEKENE EVPLLHDLVI LKNINEIYHD FYNTLFISPI EVSIPKDKRP KNGGICLMFQ LKSNDDLNAN SIIKSFFPMN SMSPNLLDKV YSSVNYNKQT FFTDEIRVQL PFPLREKYHL LITVLFISYD DFSQKPMYYS VLPLFANGNV VSSMVYSLPL LKGNLTEGYL NQLHHDVTKM SINVKIDVQS TIYPKEKNIY RFLNGKMVGV ERALESIPVQ EAIHFLPSIV NYFIFHTKEN SLGFFALFDI FAKIEGIGKS DSSRSKLLYD FTINFTIENQ EESLVSRLLW QFLMKIKDDM EKVENVMKYS WILFTILFKG IVMMTNSGKK LDSKNLSYLS IGCQKFSSLI RKKIVSNSYK GVFEGNINLA EFISRLMGII DNEPITSIII KHLITLGSCL GNDGFTRLDR TSPAFVTLGI CRTQFIATIG ISKNFILFNK PQALEVTTIE HLSDEITQKH LVITYFIREL LNIYTREGPV GKIAIDTLLS ILLRLDFDVK YQSKEDRCYI GNILFGILIY LLDEFDKIKD VHYNQFSVSE ESINDIKVMY LCMFFVLKNI SYSTMKSWLE KEIPTRIEIF LIHLRRALLF FIHFPCSINN PIEEIKDLIH TLKQSYFTQT NNRNTVMIEP INSFGKSISS KHNRHSGGLN TTKIPSYVQQ PIVKSRINKV KNLDKIIEEL SSIVMDYAEA LFDRFENSSD NGQSLIRLEE IVATTLFQVH QNDEYTNKLK DFIRLLILRH KHFLFEGTSQ FSAILLKNLL NLCNSSQESI RKNALDLVYI MVKYNFIFSQ NISRSMSDCA VSLSELKYSN ITLFRNLIDY LPSLAKEDFE TSVKQIDELR HSYWEEGQKN INSLIHLQKI NIGVEVGDLD VFLVFMNNVL SRFMKVIELI KEFHATPNNF YVILKSNIIK EIQSAMLFSQ EDINALNTIK MNIGKSLEPM KEKIDDRITQ YLNLQISLKE QKGIVDQEKE HNNKIKTSLM SIDTFFNKLP QWISLLLQDD SYKEIIENEK KNWVEWTNDI SSLKEIDCIT FSNFETLIHL IDDLRSQIKK AQQTDEEQTK VFEVLAQMNE HKMSSGINFI EQCYRKSMKD FNEINNREYD NNCIRVIDGL IEYCQFLEMK MARLRTDFDD SINKFCSFDW WCQAIESGEK LCLYCIELSQ KYQEMKDNLI KKEEWHKKEV KYAEEVLKSY IWSTVGCELA NIRMRESNDY VKAISSICKK VCCYKDKIKE WIKEAQEDYQ LLREFKEESN NFSPPLNVKT PIKRRGKKTR KYTISSVSVI TAIHHFEEDI IEEKCKLEQI EQLNKEFEQQ AIKEIEYERI RNDYAITLHY LFTATEPFIQ NPQMITQAVI KDVQTLSQRL IQAESTDDKY RKLNRYVDYS ICKEPHHSRV SKIMSLFSDK LLGSSGPSTL KKSASGIIKK KTGFHSSGGS KKTALSIEKG NQFVDAEGEF NKDAFIDVSK SQQVLFEECL NELTTTADQI LQKLADIKSL MSGSSPIQLV EKFMIFAHEY QSTARVHIAW IQRMIEEYVS YKMYCEAGIC CVHIALYLGL PESVDKRKLK EICKEPSYDD ITMEINKETL LTVVNKLKQA SDFFKMANKI QMAIYVFEVL VSLFKTMKQY KELEVCHDSM CDLYNKLETE SNVDHYYLIN KDKNHQGLVL RSILSPNQFE AVLRKQGNTS VFVEVSPMKE NQIVENFITT NTFCFDYKYE HEDDCYKISR KRDVITIAQP LPSILPQYEV VSIKQIVFEP PAVIAEKLQQ NIEFSLSSLQ YGPLPLKYIP KLIYPFEKID ILTILKCYSK IKYPRIFNLI LQTKEIIEDS VKYQTITLTQ EQKEMVEKTE ILLTTLSDEL FEIKKNEERS KL
NT Sequence
atgaagaaca aagaacaagt cgtcattgat gatatttttg aaaagaacga ttttgttaag tcattgaagt tagtaagtag tccaaggtta gtaactccac gaattaaaac atctaaacaa ataaaagatc aagaacaaca acgagtatta aatattcaat ttgatgaatt tgattcagaa gaaacggaac aattagatat ttttcagtta agaatatttt tagaatcagg tcaatttcct atttcatcta accaacattt attcgtcaaa gaacaaaatt taagagaagt tttaggaagt atgtttcttg ataaatcaag tagaatttgt agaaaagaat ttgcaaaatt ttgtaattat tttgaacaaa aaagagatga aatagaatgg aattctgttt cattaattag tttattttca caggctgata attctaatgt accaaataaa tttaaaacat atccaactaa ccatattacc cgttttgatt taattcataa acaaagacta aataaagaaa atattgtttt ggggggttca gttatggatg attatcacac actcataatg aatcaatttt atcaacaaac tacaacaatt caactcgatg ataatatcga atatattaca acagaagaaa aaataagaaa gaattcatta gtacttgaac caggaaaata caatagaaga gaagaaatgc cttgtctttt cggtacatta tttcgtgatt taaatccatc agatttaatt aaaaatgaaa ttaatgatga tgttataaaa aacaaaaata taatttatgt gattgattta aataatgtag tgtgggcagt tggttcaatt gataacttaa ttcttaattt tgctatttat gatcaaagta aaaaggaaaa agtatcaagt aattttatta tttcacctaa acaagcagat gttaatttaa taatgaataa caatgttggt gagacatgtg caatatcatt aaatgaaagt caaattaaaa atgggaagtt atggggtgta ttaactattt ataaattatt tgatggagaa agtatggaag aaatatttat agcagaaaaa gaatcacgaa acaccttaaa aattattgaa aaaatgagac aaataaatgt tactcaaaaa gcaaaaggtg ttcaatttct tcaatatatt gcaacaggag caatggcatt aaataatgat ttacttgaag atagaaagtc atattataat ttaaaattat attatgatga tagtattatt gatttatggc aatttcttgg aaatgtagaa agtggaaaga ttaaaccaat taatggttca tgggggttta aatttgaaca gttaaaccat tatacaaaag caaatttaat tattactcaa gatggagttg ttcaaaatgt tgattcacaa ccaccagaag ctagtgaaaa agaaaatgaa gaagttcctt tattacatga tcttgttata ttaaaaaata ttaatgaaat ttatcatgac ttttataata ctttatttat atctcctatt gaagttagta ttcctaaaga taaacgacca aaaaatggtg gaatttgttt aatgtttcaa ttaaaaagta atgatgattt aaatgcaaat agtattatta aatcattttt tccaatgaat tctatgtcac caaatttatt agataaagta tattcatctg ttaattataa taaacaaaca ttctttactg atgaaattag agttcaatta ccatttccat taagagaaaa atatcatctt ttaatcactg ttcttttcat ttcatatgat gacttttcac aaaaaccaat gtattattct gttcttcctt tatttgcaaa tggaaatgtt gtttcaagta tggtttattc tttaccatta ttaaaaggaa atcttactga aggatattta aatcaattac atcatgatgt aacaaaaatg agtattaatg ttaagataga tgttcaatct actatttatc ctaaagaaaa aaatatttat aggtttttaa atggaaagat ggttggagta gaaagagctc ttgagtctat tccagttcaa gaagctattc atttcttacc ttcaattgtt aattatttca tttttcatac taaagaaaat tcattaggat tttttgcttt atttgatatc tttgcaaaaa ttgaaggtat tggtaaatca gattcttcta gatcaaagtt attatatgac tttactatta attttactat tgaaaatcaa gaagaaagtc ttgtttcaag acttttatgg caatttttaa tgaaaattaa agatgatatg gaaaaagtag agaatgtaat gaaatattct tggattttat ttactatttt atttaaagga atagtgatga tgactaattc aggtaaaaag ttagattcaa agaatttatc ttatctttct attggatgtc aaaaattttc aagtttaata agaaaaaaga ttgtatctaa tagttacaaa ggggtttttg aaggaaatat taatttagct gaatttattt caaggttgat gggtattatt gataatgaac caattacttc tattattatt aaacatctta ttacattagg gtcttgtctt gggaatgatg ggtttacaag attagatcgt acaagtccag catttgttac tttaggaata tgtagaacac aatttattgc aacaattgga atttcaaaga attttatttt atttaataaa ccacaagcat tagaagttac aacaattgaa catttaagtg atgaaataac tcaaaaacat cttgtcatta catattttat tcgagaactt ttaaatattt atactagaga aggacctgtt gggaaaattg ctattgacac attattgtca attttacttc gattagattt tgatgtaaag tatcagtcaa aagaagatag atgttatatt ggaaatatcc tttttggaat attaatttat ttattggatg agtttgataa aattaaagat gtccattata atcaattcag tgtaagtgaa gaatctatta atgatattaa agtaatgtat ctttgtatgt tctttgtttt aaagaatatt tcatattcta caatgaagag ttggttagaa aaagaaattc caacgagaat agaaatattt cttattcatt taagaagagc attattattc tttattcatt tcccatgttc aattaataat ccaatagaag aaattaaaga tcttattcat acattaaaac aatcatattt tacacaaaca aataatagaa acactgtaat gattgaacca attaattctt ttggtaaatc tatttcttct aaacataata gacattctgg tggattaaat acaactaaaa taccatctta tgttcaacaa cctattgtaa agtctaggat aaataaagta aagaatcttg ataaaataat tgaagaatta agttctattg taatggacta tgctgaagct ttatttgatc gatttgaaaa ttcttcggat aatggacaat cattaattcg attagaagaa attgttgcta ctacattatt tcaagtacat caaaatgatg aatatacaaa taaattaaaa gactttatta gattattaat tcttagacat aaacatttct tatttgaagg aactagtcaa ttcagtgcaa ttttactaaa gaatttatta aatctttgta attcatctca agaatctatt cgtaagaatg ctttggattt agtttatata atggtaaagt ataattttat attctcacaa aatataagta ggtcaatgtc tgattgtgct gtgtcattat cagaattaaa atattctaac attactctct ttagaaattt aattgactat cttccttctt tagcaaaaga agattttgag acttctgtta aacaaattga tgaacttcgt catagttatt gggaagaagg tcaaaaaaat attaattctt taattcatct tcaaaaaatt aatattggag tagaagttgg ggatttagat gtatttcttg tttttatgaa taatgttctt tctaggttca tgaaagttat tgaattaata aaagaatttc atgcaactcc aaataatttt tatgtaattt taaaaagtaa tattattaaa gaaatacaat cagcaatgtt attctctcaa gaagatatta atgctttgaa tactattaaa atgaatattg gaaaaagtct tgaaccaatg aaagaaaaaa tagatgacag aattacacaa tatcttaatc ttcaaatttc tcttaaagaa caaaaaggaa ttgttgacca agaaaaagaa cataataata aaattaaaac ttctttaatg tctattgata ctttctttaa caaattacct caatggataa gtttgttact tcaagatgat tcgtacaaag aaatcattga aaatgagaaa aaaaattggg tagaatggac taatgacatt tcttctttaa aagaaattga ttgtatcaca ttctctaatt tcgagacgtt aattcatttg attgatgatt tgagatctca aattaaaaaa gcacaacaaa ctgatgaaga acaaactaaa gtctttgaag ttttagctca aatgaatgaa cataaaatgt caagtggaat taattttatt gaacaatgtt atcgtaaatc tatgaaagat tttaatgaaa ttaataatag agaatatgac aataattgta ttagagttat agatggatta attgaatatt gtcaattttt agaaatgaaa atggcaagac tacgaactga ttttgatgat tctatcaata agttttgttc atttgactgg tggtgtcagg ctatagaaag tggggaaaaa ttgtgtttat attgtattga attaagtcaa aaatatcaag aaatgaaaga taatttgatt aagaaagaag aatggcataa gaaagaagtc aaatatgcag aagaagttct taaatcatat atttggtcta ctgttggatg tgaactagca aacatacgaa tgagagaaag taatgattat gttaaagcta tatcttcaat atgtaaaaaa gtatgttgtt ataaagataa aattaaagaa tggataaaag aagctcaaga agattatcaa ttactcagag aatttaaaga agaatcaaat aatttttcac cacctctaaa tgtaaaaaca cccattaaaa gaagaggtaa aaaaacaagg aaatatacaa tatcttcagt ttctgttatc actgcaattc atcattttga agaagatatt attgaagaaa aatgtaagtt agaacaaatt gaacaattaa ataaagaatt tgaacaacaa gcaattaaag aaattgaata tgaacgaatt agaaatgact atgctattac cttacattat ctttttactg caacagaacc atttattcaa aaccctcaaa tgataacaca agcagtaatt aaagatgtac aaacactttc tcaacgatta attcaagcag aaagtactga cgataagtat agaaaactta acagatatgt tgattactct atctgtaaag aacctcatca ttcaagagta agtaaaatta tgtctttgtt tagtgataaa ttacttggtt ctagtggacc ttcaacacta aaaaaatcag caagtggaat aattaagaaa aaaactgggt ttcattcttc tggaggttca aagaaaacag ctctttcaat agaaaaagga aatcaatttg ttgatgctga aggagaattt aataaagatg cttttattga cgtatctaaa agtcaacaag ttttgtttga agaatgttta aatgaactaa caacaaccgc agatcaaatt ctacaaaaat tagctgatat taaaagttta atgagtggaa gttctccaat tcaattggtt gagaaattta tgatttttgc tcatgaatat caaagtactg caagagttca tatagcttgg attcaaagaa tgattgaaga atatgtctct tataaaatgt attgtgaagc tggcatttgt tgtgttcata tagctttata tcttggactt ccagaaagtg ttgacaaaag aaaattaaaa gaaatatgta aggaacctag ttatgatgat attactatgg aaataaataa agaaactctt cttactgttg ttaataaatt aaaacaagca tcagacttct ttaaaatggc aaataaaata caaatggcta tctacgtttt tgaagtatta gtttctttat ttaaaacaat gaaacaatat aaagaattag aagtttgtca tgacagtatg tgtgatttat acaataaact tgaaacagag tcaaatgttg atcattatta tcttattaat aaagacaaaa accatcaagg attagtactt cgctctatcc tttcaccaaa tcaatttgaa gctgttttaa gaaaacaagg aaatacttct gtttttgttg aagttagtcc aatgaaagaa aaccaaattg tagaaaactt cattacaaca aatactttct gttttgatta taaatatgaa catgaagatg actgctataa aatatctcgg aaacgagatg ttatcactat tgcccaacca ctcccttcta tactaccaca atatgaagtg gtttctatta aacaaattgt ctttgaacca ccagctgtta ttgctgaaaa attacaacaa aatattgaat tttctttatc ttctttacag tatggaccat taccattaaa gtatattcca aagttaattt atccatttga aaaaatagat attttaacaa tattaaaatg ttattcaaaa ataaaatatc ctcgcatttt caatttaata cttcaaacaa aagaaattat tgaagactca gtcaaatacc aaaccattac cttaacccaa gaacaaaaag aaatggttga aaaaacagaa atacttctta ctactctttc tgatgaatta tttgaaataa aaaagaatga ggaaagaagc aaattataa
Details for EnhiA.18437.a.B5.GE35781
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/6/2012
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 47
Validated AA Sequence
MWCQAIESGE KLCLYCIELS QKYQEMKDNL IKKEEWHKKE VKYAEEVLKS YIWSTVGCEL ANIRMRESND YVKAISSICK KVCCYKDKIK EWIKEAQEDY QLLREFKEES NNFSPPLNVK TPIKRRGKKT RKYTISSVSV IXXXIILKKI LLKKNVS
Validated NT Sequence
attttnnttn ntttaagaag gaganatacc ntggctcacc accaccacca ccatatgtgg tgtcaggcta tagaaagtgg ggaaaaattg tgtttatatt gtattgaatt aagtcaaaaa tatcaagaaa tgaaagataa tttgattaag aaagaagaat ggcataagaa agaagtcaaa tatgcagaag aagttcttaa atcatatatt tggtctactg ttggatgtga actagcaaac atacgaatga gagaaagtaa tgattatgtt aaagctatat cttcaatatg taaaaaagta tgttgttata aagataaaat taaagaatgg ataaaagaag ctcaagaaga ttatcaatta ctcagagaat ttaaagaaga atcaaataat ttttcaccac ctctaaatgt aaaaacaccc attaaaagaa gaggtaaaaa aacaaggaaa tatacaatat cttcagtttc tgttatcann nnnntcatca ttttgaagaa gatattattg aagaaaaatg taagttagaa caaattgaac aattaaataa agaatttgaa caacaagcaa ttaaagaaat tgaatatgaa cgaattagaa atgactatgc tattacctta cattatcttt ttactgcaac agaaccattt attcaaaacc ctcaaatgat aacacaagca gtaattaaag atgtacaaac actttctcaa cgattaattc aagcagaaag tactgacgan nntatagaaa acttaacaga tatgttgatt actctatctg taaagaacct catcattcna gagtaagtaa aattatgtct ttgtttagtg ataaattact nggttctagt ggnncttcaa cactaaaaaa atcagcaagt ggnataatta agaaaaaaac tgggtttcat tcttctggag gtcaaagaaa acagctcnnc aatagaaaan nnantcantt tgttgangcn gaannnantt annnaannnn cttttnantg acgtannnan ntcacagttt nnannnanna nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnannnnnn nnnaannann nnanaannnn nnnnnnna
Expected Protein Sequence
MAHHHHHHMW CQAIESGEKL CLYCIELSQK YQEMKDNLIK KEEWHKKEVK YAEEVLKSYI WSTVGCELAN IRMRESNDYV KAISSICKKV CCYKDKIKEW IKEAQEDYQL LREFKEESNN FSPPLNVKTP IKRRGKKTRK YTISSVSVIT AIHHFEEDII EEKCKLEQIE QLNKEFEQQA IKEIEYERIR NDYAITLHYL FTATEPFIQN PQMITQAVIK DVQTLSQRLI QAESTDDKYR KLNRYVDYSI CKEPHHSRVS KIMSLFSDKL LGSSGPSTLK KSASGIIKKK TGFHSSGGSK KTALSIEKGN QFVDAEGEFN KDAFIDVSKS QQVL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtggt gtcaggctat agaaagtggg gaaaaattgt gtttatattg tattgaatta agtcaaaaat atcaagaaat gaaagataat ttgattaaga aagaagaatg gcataagaaa gaagtcaaat atgcagaaga agttcttaaa tcatatattt ggtctactgt tggatgtgaa ctagcaaaca tacgaatgag agaaagtaat gattatgtta aagctatatc ttcaatatgt aaaaaagtat gttgttataa agataaaatt aaagaatgga taaaagaagc tcaagaagat tatcaattac tcagagaatt taaagaagaa tcaaataatt tttcaccacc tctaaatgta aaaacaccca ttaaaagaag aggtaaaaaa acaaggaaat atacaatatc ttcagtttct gttatcactg caattcatca ttttgaagaa gatattattg aagaaaaatg taagttagaa caaattgaac aattaaataa agaatttgaa caacaagcaa ttaaagaaat tgaatatgaa cgaattagaa atgactatgc tattacctta cattatcttt ttactgcaac agaaccattt attcaaaacc ctcaaatgat aacacaagca gtaattaaag atgtacaaac actttctcaa cgattaattc aagcagaaag tactgacgat aagtatagaa aacttaacag atatgttgat tactctatct gtaaagaacc tcatcattca agagtaagta aaattatgtc tttgtttagt gataaattac ttggttctag tggaccttca acactaaaaa aatcagcaag tggaataatt aagaaaaaaa ctgggtttca ttcttctgga ggttcaaaga aaacagctct ttcaatagaa aaaggaaatc aatttgttga tgctgaagga gaatttaata aagatgcttt tattgacgta tctaaaagtc aacaagtttt gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat