SSGCID: Target Page

EnhiA.18429.a

Chitinase Jessie 3 (Chitinase Jessie 3, putative)

CENTER ID:	EnhiA.18429.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.18429.a.B2.GE35776	region1		24	336
EnhiA.18429.a.B3.GE35777	region2		337	621

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_152170
RefSeq:	XP_657334.2
UniProt:	Q962P2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKLAVLTVTI FMTLSYGLNI TFSQRTNCDG LESGFYCVDS TTYYWCYGQS RFKQATCPNG LECKCGKTIY NPCMWPFQGN TPDCDGKPGK YFSGTTPEEP SESSTVNPPE DSSSIPPSPD SSSTPVSSSS HTPEPSESSS QQKSESSTSQ SSNNSGDDDY NPDWPDVEKG VYSMAPNTHL PLVLKFDKNK WKEQIKEVVS NSNYNNEALY QPATSDYACA LHPPSKYDNN PTQIWIGRPS QSVTISYTPG VAIRLPDKYY GMFLGYAMDA YGLNPGMLIG LGAKESFSFA RYQATDDGSY FIVANENEHY DCYSSSQRGL CRDGNLDGPF QVETGGMSTD VAILPNRFWI GESTTPKSER KIKYMFDNEV LTYAGFRAYH DYFTLNYGRA FVLTSLDFHF RHNLVMNMKK VGLRDALSKR TKREQRDSLE FATAMYTYNR GVFDTQLIQM LNGCNADMDP CTDCKLDGYG GHTTDIRIVC KAVDSVPNKE VYDYNLSKED VEYFISTIQS TYPFNNVDWK TLRSDVDTAY DYLKSQRNSN AISFRYDWRT LLAVIRMHLP PIEYFVGDQV KTFQDYWGGV LNDDLGPYQS VSNFPFKFCS TSGGRSNLCS SKSHKQVHYS K

NT Sequence

atgaaattag ctgttttaac agttactata ttcatgactt taagttatgg tttaaatatc acatttagtc aacgaaccaa ttgtgatggt cttgaaagtg ggttttattg tgttgactca acaacatatt attggtgtta tggacaatcc agatttaagc aagcaacgtg tcctaatggt ttagaatgca aatgtggtaa aacaatttac aacccatgca tgtggccgtt tcaaggaaac acaccagatt gtgatggaaa accaggaaag tatttcagtg gtacaacacc agaagaacca tcagaaagct caacagttaa tcctccagaa gattcatctt caattccacc atcacctgac tcatcttcta ctcctgtaag ttcaagttca catactccag aaccatcaga gtcatctagt caacaaaaat cagagtcatc tacatcccaa tcatctaaca attcaggaga tgatgactat aatcctgact ggccagatgt tgaaaaagga gtatattcca tggcaccaaa tactcatctt ccattagtac taaaatttga caaaaacaaa tggaaagaac aaatcaaaga agttgtttca aattcaaatt ataacaatga agcattatat caaccagcaa catcagacta tgcctgtgct cttcacccac catcaaaata tgataataat ccaacacaaa tatggattgg aagaccaagt caatcagtaa caatttcata tactccagga gtagcaatcc gattaccaga caaatattat ggaatgtttt tgggatatgc catggatgct tatggattaa atccaggaat gcttattgga cttggtgcaa aagaatcatt tagttttgca cgatatcaag caactgatga tggaagttat ttcattgttg ccaatgaaaa tgaacactat gactgctact catcatcaca aagaggactt tgtcgtgatg gaaatttaga tggtccattc caagttgaaa caggaggaat gtcaactgat gttgcaatat taccaaatag attctggatt ggtgaatcaa ctacaccaaa gtctgaaaga aagattaaat acatgtttga taatgaggtt ttaacatatg caggatttag agcatatcat gattatttca cattaaatta tggaagggca tttgtattaa cttctttaga tttccatttc aggcataatt tagtaatgaa catgaagaag gttggattaa gagacgcatt gtctaaacga accaaacgag aacaacgaga ctctcttgaa tttgctactg ctatgtatac atacaataga ggtgtttttg atactcaact aatccaaatg ctaaatggat gtaatgcaga tatggatcca tgcacggatt gtaaattaga tggatatgga ggacatacca ctgacattag aattgtttgt aaagctgttg attcagttcc aaataaagag gtgtatgatt ataatttgag taaagaagat gttgaatatt tcattagtac aattcaatca acatatccat ttaataacgt agattggaaa acacttagaa gtgatgttga cactgcctat gattatctaa aatcccaaag aaatagtaat gcaatttctt tcagatatga ttggagaaca ttacttgccg taataagaat gcatcttcca cctattgaat attttgttgg tgaccaagtc aaaacattcc aagattattg gggaggtgta ttgaatgatg acctaggtcc ataccaatca gtttctaatt tcccatttaa attctgttct acttctggag gaagatctaa tttatgttct tctaagtcac acaaacaagt tcattattca aaataa

Details for EnhiA.18429.a.B2.GE35776

HARVESTED ON:	7/31/2012
SEQUENCED ON:	8/3/2012
EXPECTED MW:	35kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	93
PERCENT COVERAGE:	103

Validated AA Sequence

MAHHHHHHMQ RTNCDGLESG FYCVDSTTYY WCYGQSRFKQ ATCPNGLECK CGKTIYNPCM WPFQGNTPDC DGKPGKYFSG TTPEEPSESS TVNPPEDSSS IPPSPDSSST PVSSSSHTPE PSESSSQQKS ESSTSQSSNN SGDDDYNPDW PDVEKGVYSM APNTHLPLVL KFDKNKWKEQ IKEVVSNSNY NNEALYQPAT SDYACALHPP SKYDNNPTQI WIGRPSQSVT ISYTPGVAIR LPDKYYGMFL GYAMDAYGLN PGMLIGLGAK ESFSFARYQA TDDGSYFIVA XENEHYDCYS SSXXDFVVME IXMVHSXLKX XXSEXXXXXX XXX

Validated NT Sequence

ttttgtnnnn tttaagaagg aganatacca tggctcacca ccaccaccac catatgcaac gaaccaattg tgatggtctt gaaagtgggt tttattgtgt tgactcaaca acatattatt ggtgttatgg acaatccaga tttaagcaag caacgtgtcc taatggttta gaatgcaaat gtggtaaaac aatttacaac ccatgcatgt ggccgtttca aggaaacaca ccagattgtg atggaaaacc aggaaagtat ttcagtggta caacaccaga agaaccatca gaaagctcaa cagttaatcc tccagaagat tcatcttcaa ttccaccatc acctgactca tcttctactc ctgtaagttc aagttcacat actccagaac catcagagtc atctagtcaa caaaaatcag agtcatctac atcccaatca tctaacaatt caggagatga tgactataat cctgactggc cagatgttga aaaaggagta tattccatgg caccaaatac tcatcttcca ttagtactaa aatttgacaa aaacaaatgg aaagaacaaa tcaaagaagt tgtttcaaat tcaaattata acaatgaagc attatatcaa ccagcaacat cagactatgc ctgtgctctt cacccaccat caaaatatga taataatcca acacaaatat ggattggaag accaagtcaa tcagtaacaa tttcatatac tccaggagta gcaatccgat taccagacaa atattatgga atgtttttgg gatatgccat ggatgcttat ggattaaatc caggaatgct tattggactt ggtgcaaaag aatcatttag ttttgcacga tatcaagcaa ctgatgatgg aagttatttc attgttgccn atgaaaatga acactatgac tgctactcat catcacanna ggactttgtc gtgatggaaa tttanatggt ccattccnag ttgaaacngn nnntaagtga gtananagna nccnntnnnn nnnncnccna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaac gaaccaattg tgatggtctt gaaagtgggt tttattgtgt tgactcaaca acatattatt ggtgttatgg acaatccaga tttaagcaag caacgtgtcc taatggttta gaatgcaaat gtggtaaaac aatttacaac ccatgcatgt ggccgtttca aggaaacaca ccagattgtg atggaaaacc aggaaagtat ttcagtggta caacaccaga agaaccatca gaaagctcaa cagttaatcc tccagaagat tcatcttcaa ttccaccatc acctgactca tcttctactc ctgtaagttc aagttcacat actccagaac catcagagtc atctagtcaa caaaaatcag agtcatctac atcccaatca tctaacaatt caggagatga tgactataat cctgactggc cagatgttga aaaaggagta tattccatgg caccaaatac tcatcttcca ttagtactaa aatttgacaa aaacaaatgg aaagaacaaa tcaaagaagt tgtttcaaat tcaaattata acaatgaagc attatatcaa ccagcaacat cagactatgc ctgtgctctt cacccaccat caaaatatga taataatcca acacaaatat ggattggaag accaagtcaa tcagtaacaa tttcatatac tccaggagta gcaatccgat taccagacaa atattatgga atgtttttgg gatatgccat ggatgcttat ggattaaatc caggaatgct tattggactt ggtgcaaaag aatcatttag ttttgcacga tatcaagcaa ctgatgatgg aagttatttc attgttgcca atgaaaatga acactatgac tgctactcat catcacaaag aggactttgt cgtgatggaa atttagatgg tccattccaa gttgaaacag gaggatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for EnhiA.18429.a.B3.GE35777

HARVESTED ON:	7/31/2012
SEQUENCED ON:	8/3/2012
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	94
PERCENT COVERAGE:	104

Validated AA Sequence

MAHHHHHHMS TDVAILPNRF WIGESTTPKS ERKIKYMFDN EVLTYAGFRA YHDYFTLNYG RAFVLTSLDF HFRHNLVMNM KKVGLRDALS KRTKREQRDS LEFATAMYTY NRGVFDTQLI QMLNGCNADM DPCTDCKLDG YGGHTTDIRI VCKAVDSVPN KEVYDYNLSK EDVEYFISTI QSTYPFNNVD WKTLRSDVDT AYDYLKSQRN SNAISFRYDW RTLLAVIRMH LPPIEYFVGD QVKTFQDYWG XVLNDDLXPY XSVSNFPFXX CSTSXGRXNL CSSXHTXSSL XXXXXXXGXR LLXXXXX

Validated NT Sequence

tttgtttnct ttnnnaagga ganataccat ggctcaccac caccaccacc atatgtcaac tgatgttgca atattaccaa atagattctg gattggtgaa tcaactacac caaagtctga aagaaagatt aaatacatgt ttgataatga ggttttaaca tatgcaggat ttagagcata tcatgattat ttcacattaa attatggaag ggcatttgta ttaacttctt tagatttcca tttcaggcat aatttagtaa tgaacatgaa gaaggttgga ttaagagacg cattgtctaa acgaaccaaa cgagaacaac gagactctct tgaatttgct actgctatgt atacatacaa tagaggtgtt tttgatactc aactaatcca aatgctaaat ggatgtaatg cagatatgga tccatgcacg gattgtaaat tagatggata tggaggacat accactgaca ttagaattgt ttgtaaagct gttgattcag ttccaaataa agaggtgtat gattataatt tgagtaaaga agatgttgaa tatttcatta gtacaattca atcaacatat ccntttaata acgtagattg gaaaacactt agaagtgatg ttgacactgc ctatgattat ctaaaatccc aaagaaatag taatgcaatt tctttcagat atgattggag aacattactt gccgtaataa gaatgcatct tccacctatt gaatattttg ttggtgacca agtcaaaaca ttccaagatt attggggang tgtattgaat gatgacctan gtccataccn atcagtttct aatttcccat ttnanntctg ttctacttct gnaggaagan ctaatttatg ttcttctant cacacnncna gttcattant cnaanaantg antnananag gatnccggct gctaannann nccnaaagna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcaa ctgatgttgc aatattacca aatagattct ggattggtga atcaactaca ccaaagtctg aaagaaagat taaatacatg tttgataatg aggttttaac atatgcagga tttagagcat atcatgatta tttcacatta aattatggaa gggcatttgt attaacttct ttagatttcc atttcaggca taatttagta atgaacatga agaaggttgg attaagagac gcattgtcta aacgaaccaa acgagaacaa cgagactctc ttgaatttgc tactgctatg tatacataca atagaggtgt ttttgatact caactaatcc aaatgctaaa tggatgtaat gcagatatgg atccatgcac ggattgtaaa ttagatggat atggaggaca taccactgac attagaattg tttgtaaagc tgttgattca gttccaaata aagaggtgta tgattataat ttgagtaaag aagatgttga atatttcatt agtacaattc aatcaacata tccatttaat aacgtagatt ggaaaacact tagaagtgat gttgacactg cctatgatta tctaaaatcc caaagaaata gtaatgcaat ttctttcaga tatgattgga gaacattact tgccgtaata agaatgcatc ttccacctat tgaatatttt gttggtgacc aagtcaaaac attccaagat tattggggag gtgtattgaa tgatgaccta ggtccatacc aatcagtttc taatttccca tttaaattct gttctacttc tggaggaaga tctaatttat gttcttctaa gtcacacaaa caagttcatt attcaaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat