EnhiA.18428.a

Clathrin heavy chain (Fragment)

CENTER ID: EnhiA.18428.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.18428.a.B2.GE35749 region1 1 319
EnhiA.18428.a.B3.GE35750 region2 400 736
EnhiA.18428.a.B4.GE35751 region3 753 886
EnhiA.18428.a.B5.GE35752 region4 848 1013
EnhiA.18428.a.B6.GE35753 region5 1010 1158
EnhiA.18428.a.B7.GE35754 region6 1114 1448
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.18428.a.B3.PS01634 region2 400 736

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_201510
UniProt: Q1EQ28

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAIRPPIQIK EVVKLDRFVK PESIGFTTVS IEGDKHFTVL EKGEEKRVRI FHNNKINSPD SRSCAADFAI MHPTKQIMAV AAGTTVQMLI PSAAPKKIVD RHSELAASQI IGYKIDPTEK WVAILALLQK NNEIVGKIQL FSIEKNASQI IDGYAASFYN YKFDGASQAS VMFAFAQKFP SPRLGLIEVI KGDPAKQNVN TVIDIPIQPQ QQQADFMVGL VVSVKYETIL LVSRMGYIYG FYSGDGTLFF QGRVIDDIVF LTAYNQSTDG VTVVGKKGVV TNIYIEGDKL VPFICNGLKN VGLGCKIAAR CNLPGADDIF VERFNALLNA GQIREAALIA ANSPGELIRN ANTINKLSQM QAQPGQNPPL FLYFATILES SSLNALEGFT LIQLLIPQNK LPAVEAWIVN DKIECSEKAG DLVKPYDQRL ALMIYSKAHA TGKVVQSLAE MGQSDKIVAY CERAGFSPDY LQILQQILPA SGEAAKAYAA SLIQQKGYDE LDMISIIDLF ARYRYLKEIT ALILDTLDGD NEQYANIQTK VLEMNLIGAP QIADTIFEND MLKHFDHNRI GKLCEQAGLF KRALQIFEQF DDIRRVLAHA SAIPPELIIE SFRKLEPEQA ILVLQDLLRT NPRGNLQIII KILLEFHTGL GDDKVIDLLQ KFDCWEGLFY FLSQIVNTTN DKMVVFKYIE ACARSGQFQE LARVVQENDV YDPKEVKEFL KNAKIADQIP FIIVCDKHGY VEEMTQYLYQ NGNHRFIEAY VKKINPMNTP KVVGALLDCG CGEDYIQQLI NAVGGICPAD ELIKTCESRN RLIILLPWLE QRLSEGSPDV SLHTALAKVY VDQGKDAEKF LNNDMYYDSL IVGKYCEDRD PYLSYICYKR KNLDDELIDV TNRHNLFKYQ AKYLVEREDL DLWNKVLTEE NEHRKSVVEQ VIHTALPECT DPNQILKTVQ AFLQNDLPND LIDLLDKLVS QNPEYSTNPT LQKLLIQTSI KAAPNRVMDY IRKLNDYDPK EIAMDVLLQH IKDLTRAKDF ADKAKKDILY KKLGEAQLDE LKVKDAIISL MKAKDISLRS RVIDIAESDG SYEDLINYLL MCKEETKDMM VETELLYCYA KLKKNDEIEN FLKTANCANL TSIAERCYNE ELYGAAKILY TSLNNYIKLA SCLLKLKDYA GAVEAAKKAN STRTWKEVTF ACIDAKEFTL AQETGINILM AGDEITELVY YYEKNELYDQ VIELLEAGLK IENVHVSMFT ELAILYSKYK EEKLYDYLKQ YVAKIQCQKV IPTVNMNQQW KELVFLYVQV DQVKAIETMI SYPDDCFDHQ LMKELLVNVP RIDMIYKAES YYLAEKPEKV NEMLIAVAHR CDHTQVISIA RKEKDLKTIR EYLLYCLDLN NDSVNEALID LFIDDGDAKS LKSLIEKNTN FNKTGLANRL KVHEDVEFRK IAAYLYSRFD DDAMEIAKKS KDEEKVTDLL NFFVEQDKKE AFGNCLNVCY DYVPTEVALE LGYKNKLMDQ TMPFMCKKMK DTNDRIKKLE QAEKDRQNAK KEPQLAQQDT PFGMLALPAA PGMMPMQQPM GGMGMMNQPM PGMAPSVQPQ TAMGGMGMMN QPMPGMTPMG AAAPTTNGEF IF
NT Sequence
atggctatta gaccaccaat tcaaataaaa gaagtagtta aattagacag atttgtcaaa ccagaaagta ttggatttac tacagtttcc attgaaggag ataaacattt tacagtatta gaaaagggag aagagaaaag agtaagaata ttccataata ataagattaa cagtcctgat tcacgatcat gtgctgctga ttttgctatt atgcatccaa caaaacaaat tatggcagtt gctgcaggaa ctactgttca aatgttgatt ccaagtgcag caccaaagaa aatagttgat agacattcag agcttgcagc atcacaaatt attggatata aaattgatcc aacagaaaaa tgggttgcta tacttgcatt attacaaaaa aataatgaaa ttgttggtaa aattcaattg ttttctattg aaaagaatgc atcacaaatt atagatgggt atgcagctag tttttataat tataaatttg atggagcatc acaagcatca gttatgtttg cctttgcaca aaaattccca tcaccaagac ttggattgat tgaagttatt aaaggagatc cagctaaaca aaatgtaaat acagtaattg atatacctat tcaaccacaa caacaacaag cagactttat ggtaggatta gttgtttcag taaaatatga aacaatatta ttagtatcaa gaatgggata tatttatgga ttttattcag gagatggtac attattcttc caaggaagag tgattgatga tattgtgttc ttaactgcat acaatcaaag cacagatgga gtaactgtag taggaaagaa aggagttgta actaatattt atattgaagg agataaatta gttccattca tttgtaatgg attaaaaaat gttggacttg gatgtaaaat tgcagctcga tgtaatcttc caggagctga tgatattttt gtagaaagat ttaatgcact tttaaatgca ggacaaatta gagaagctgc tcttattgca gctaattcac caggtgaatt aattagaaat gcaaatacaa ttaataaatt aagtcaaatg caagcacaac caggacaaaa tcctccatta ttcctttatt ttgctactat tttagaaagt agttcactta atgcacttga aggatttact cttattcaat tacttattcc acaaaataaa cttccagcag ttgaggcatg gattgtaaat gataaaattg aatgcagtga aaaagcagga gatttagtta agccatatga tcaacgactt gcattaatga tttattctaa agcacatgct acaggaaaag tagttcaatc attagcagaa atgggacaaa gtgataaaat agttgcttat tgtgaaagag caggattttc accagactat cttcaaattc ttcaacaaat tcttccagca tcaggagaag ctgctaaggc atatgctgca agtttaattc aacaaaaagg atatgatgaa ttagatatga tttcaataat agatcttttt gctagatata gatatcttaa agaaattact gcattaatac ttgatactct tgatggagat aatgaacaat atgctaatat tcaaacaaaa gttcttgaaa tgaatttaat tggagcccca caaattgctg atactatttt tgagaatgat atgttaaaac attttgatca taatagaatt ggtaaattgt gtgaacaagc tggattattt aaacgagctc ttcaaatttt tgaacaattt gatgatatta gaagagttct tgcacatgca tctgctattc caccagaatt aattattgaa agttttagaa aattagaacc agaacaagct attcttgttc ttcaagacct tttaagaaca aatccaagag gaaatcttca aattattatt aaaatattac ttgaattcca tacaggactt ggagatgata aagtcattga tttacttcaa aagtttgatt gttgggaagg attattctat ttcttatctc aaattgtgaa tactactaat gacaaaatgg ttgtatttaa atatattgag gcatgtgcca gatcaggaca attccaagaa ttagcacgtg ttgttcaaga gaatgatgta tatgatccaa aagaagttaa agaattctta aagaatgcaa aaattgcaga ccaaattcca tttattattg tatgtgataa acatggttat gttgaagaaa tgacacaata tctttatcaa aatggaaatc atagatttat tgaagcatat gttaagaaaa ttaatccaat gaatacacca aaagttgttg gtgcattatt agattgtgga tgtggagaag attatattca acaattaatt aatgcagttg gaggtatttg tccagctgat gaattgatta agacttgtga aagtagaaat agattaatta ttttattacc atggttagaa caaagattat ctgaaggatc tcctgatgtt tctcttcata cagctcttgc taaagtttat gttgaccaag gaaaagatgc agagaaattc ttaaataatg atatgtatta tgactcatta attgtaggta aatattgtga agatagagat ccatatttat catacatttg ttataagaga aagaatttgg atgatgaatt gattgatgta actaatagac ataatttatt caaataccaa gcaaaatatc ttgttgaaag agaagatctt gatttatgga ataaagtctt gacagaagaa aatgaacata gaaagagtgt tgttgaacaa gttattcata ctgcacttcc agaatgtact gatccaaatc aaattctcaa aacagttcaa gcattccttc aaaatgacct tccaaatgat ttgattgatt tattggataa attagtttct caaaatccag aatatagcac taatccaaca ttacaaaaat tattgattca aactagtatt aaagcagcac caaatagagt tatggattat attagaaaac ttaatgacta tgatccaaaa gaaattgcta tggatgtatt attacaacac attaaagacc ttactcgtgc taaagacttt gctgataaag ctaaaaaaga tattttatat aagaaacttg gagaagcaca acttgatgaa ttgaaagtta aagacgctat tatttcattg atgaaagcta aggacattag tttaagatca agagttattg atattgcaga gtcagatgga tcatatgaag atttaattaa ttacttactt atgtgtaagg aagaaactaa agacatgatg gttgaaactg aattattata ttgttatgct aaattgaaaa agaatgatga aattgaaaac ttccttaaaa cagctaattg tgcaaatctt acctccattg ctgaaagatg ttataatgaa gaattatatg gagctgctaa gattctttat acatcattaa ataattatat taaattagca tcatgcttac ttaaattaaa ggattatgct ggagctgtag aagctgctaa aaaggctaat agtactagaa catggaaaga agttacattt gcatgtattg atgcaaaaga atttacatta gcacaagaaa caggaattaa tatcttaatg gctggtgatg aaattactga attagtttat tattatgaaa agaatgaatt atatgatcaa gtcattgaat tacttgaagc aggattaaag attgaaaatg ttcatgtatc tatgtttact gaattagcta ttctttattc taaatataaa gaagaaaagt tatatgatta tcttaaacaa tatgttgcta agattcaatg ccaaaaagtt attccaaccg tcaatatgaa tcaacaatgg aaagaacttg tattccttta tgttcaagtt gatcaagtta aagctattga gactatgatt tcatatccag atgattgttt cgatcatcaa ttaatgaaag aattattagt taatgttcca agaattgata tgatttataa agctgaatca tattatcttg cagaaaaacc agaaaaggtt aatgaaatgt taattgctgt agctcataga tgtgaccata ctcaagttat ttctattgct cgtaaagaaa aagatcttaa aactattaga gaatatcttc tttattgtct tgatttaaat aatgattcag tcaatgaagc acttattgat cttttcattg atgatggaga tgctaagagc ttaaagagtc ttatcgaaaa gaatacaaac ttcaacaaaa ctggattggc taatagattg aaagttcatg aagatgttga atttagaaaa attgcagcat atctttatag tagatttgat gatgatgcta tggaaattgc taagaaatcc aaagatgaag aaaaggttac agatttattg aacttctttg tagaacaaga caagaaagaa gcctttggaa attgtttgaa tgtttgttat gattatgttc caacagaagt agcacttgaa cttggatata agaataaatt aatggatcaa acaatgccat tcatgtgtaa gaaaatgaag gatactaatg atagaattaa aaaacttgaa caagcagaaa aagatagaca aaatgctaaa aaagaaccac aacttgctca acaagatact ccattcggta tgttagctct tcctgctgca ccaggtatga tgccaatgca acaaccaatg ggaggaatgg gtatgatgaa ccaaccaatg ccaggaatgg caccttctgt tcaaccacaa actgctatgg gaggaatggg tatgatgaat caacctatgc caggaatgac tccaatggga gctgcagcac caactacaaa tggtgaattc attttctaa
Details for EnhiA.18428.a.B2.GE35749
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/6/2012
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 88
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA IRPPIQIKEV VKLDRFVKPE SIGFTTVSIE GDKHFTVLEK GEEKRVRIFH NNKINSPDSR SCAADFAIMH PTKQIMAVAA GTTVQMFDVQ SKSKVADITL QTPLVFWKWA NEHILAIVTS TSVFHFDLNS PSAAPKKIVD RHSELAASQI IGYKIDPTEK WVAILALLQK NNEIVGKIQL FSIEKNASQI IDGYAASFYN YKFDGASQAS VMFAFAQKFP SPRLGLIEVI KGDPAKQNVN TVIDIPIQPQ QQQADFMVGL VVSVKYETIL LVSRMGYIYG FYSGDGTLFF QGRVIDDIVF LTAYNQSTDG VTVVGKKGVV TNIYIEGDKL VPFICNGLKN VGLGCKIAAR CNLPGADDI
Validated NT Sequence
ttaagaagga ganataccat ggctcaccac caccaccacc atatggctat tagaccacca attcaaataa aagaagtagt taaattagac agatttgtca aaccagaaag tattggattt actacagttt ccattgaagg agataaacat tttacagtat tagaaaaggg agaagagaaa agagtaagaa tattccataa taataagatt aacagtcctg attcacgatc atgtgctgct gattttgcta ttatgcatcc aacaaaacaa attatggcag ttgctgcagg aactactgtt caaatgtttg atgttcaatc taaatccaaa gtagcagata taacacttca aacaccatta gtattttgga aatgggctaa tgaacatatt ttagctattg taacatctac atcagtattt cattttgatt taaatagtcc aagtgcagca ccaaagaaaa tagttgatag acattcagag cttgcagcat cacaaattat tggatataaa attgatccaa cagaaaaatg ggttgctata cttgcattat tacaaaaaaa taatgaaatt gttggtaaaa ttcaattgtt ttctattgaa aagaatgcat cacaaattat agatgggtat gcagctagtt tttataatta taaatttgat ggagcatcac aagcatcagt tatgtttgcc tttgcacaaa aattcccatc accaagactt ggattgattg aagttattaa aggagatcca gctaaacaaa atgtaaatac agtaattgat atacctattc aaccacaaca acaacaagca gactttatgg taggattagt tgtttcagta aaatatgaaa caatattatt agtatcaaga atgggatata tttatggatt ttattcagga gatggtacat tattcttcca aggaagagtg attgatgata ttgtgttctt aactgcatac aatcaaagca cagatggagt aactgtagta ggaaagaaag gagttgtaac taatatttat attgaaggag ataaattagt tccattcatt tgtaatggat taaaaaatgt tggacttgga tgtaaaattg cagctcgatg taatcttcca ggagctgatg atatttaagt gagtaagata ggatccggct gctaacaaag c
Expected Protein Sequence
MAHHHHHHMA IRPPIQIKEV VKLDRFVKPE SIGFTTVSIE GDKHFTVLEK GEEKRVRIFH NNKINSPDSR SCAADFAIMH PTKQIMAVAA GTTVQMLIPS AAPKKIVDRH SELAASQIIG YKIDPTEKWV AILALLQKNN EIVGKIQLFS IEKNASQIID GYAASFYNYK FDGASQASVM FAFAQKFPSP RLGLIEVIKG DPAKQNVNTV IDIPIQPQQQ QADFMVGLVV SVKYETILLV SRMGYIYGFY SGDGTLFFQG RVIDDIVFLT AYNQSTDGVT VVGKKGVVTN IYIEGDKLVP FICNGLKNVG LGCKIAARCN LPGADDI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta ttagaccacc aattcaaata aaagaagtag ttaaattaga cagatttgtc aaaccagaaa gtattggatt tactacagtt tccattgaag gagataaaca ttttacagta ttagaaaagg gagaagagaa aagagtaaga atattccata ataataagat taacagtcct gattcacgat catgtgctgc tgattttgct attatgcatc caacaaaaca aattatggca gttgctgcag gaactactgt tcaaatgttg attccaagtg cagcaccaaa gaaaatagtt gatagacatt cagagcttgc agcatcacaa attattggat ataaaattga tccaacagaa aaatgggttg ctatacttgc attattacaa aaaaataatg aaattgttgg taaaattcaa ttgttttcta ttgaaaagaa tgcatcacaa attatagatg ggtatgcagc tagtttttat aattataaat ttgatggagc atcacaagca tcagttatgt ttgcctttgc acaaaaattc ccatcaccaa gacttggatt gattgaagtt attaaaggag atccagctaa acaaaatgta aatacagtaa ttgatatacc tattcaacca caacaacaac aagcagactt tatggtagga ttagttgttt cagtaaaata tgaaacaata ttattagtat caagaatggg atatatttat ggattttatt caggagatgg tacattattc ttccaaggaa gagtgattga tgatattgtg ttcttaactg catacaatca aagcacagat ggagtaactg tagtaggaaa gaaaggagtt gtaactaata tttatattga aggagataaa ttagttccat tcatttgtaa tggattaaaa aatgttggac ttggatgtaa aattgcagct cgatgtaatc ttccaggagc tgatgatatt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for EnhiA.18428.a.B3.GE35750
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/6/2012
EXPECTED MW: 39kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MKLPAVEAWI VNDKIECSEK AGDLVKPYDQ RLALMIYSKA HATGKVVQSL AEMGQSDKIV AYCERAGFSP DYLQILQQIL PASGEAAKAY AASLIQQKGY DELDMISIID LFARYRYLKE ITALILDTLD GDNEQYANIQ TKVLEMNLIG APQIADTIFE NDMLKHFDHN RIGKLCEQAG LFKRALQIFE QFDDIRRVLA HASAIPPELI IESFRKLEPE QAILVLQDLL RTNPRGNLQI IIKILLEFHT GLGDDKVIDL LQKFDCWEGL FYFLSQIVNT TNDKMVVFKY IEACARSGQF QELARVVQEN DVYDPKEVKE FLKNAKIADQ IPFIIVCD
Validated NT Sequence
ccaccaccac catatgaaac ttccagcagt tgaggcatgg attgtaaatg ataaaattga atgcagtgaa aaagcaggag atttagttaa gccatatgat caacgacttg cattaatgat ttattctaaa gcacatgcta caggaaaagt agttcaatca ttagcagaaa tgggacaaag tgataaaata gttgcttatt gtgaaagagc aggattttca ccagactatc ttcaaattct tcaacaaatt cttccagcat caggagaagc tgctaaggca tatgctgcaa gtttaattca acaaaaagga tatgatgaat tagatatgat ttcaataata gatctttttg ctagatatag atatcttaaa gaaattactg cattaatact tgatactctt gatggagata atgaacaata tgctaatatt caaacaaaag ttcttgaaat gaatttaatt ggagccccac aaattgctga tactattttt gagaatgata tgttaaaaca ttttgatcat aatagaattg gtaaattgtg tgaacaagct ggattattta aacgagctct tcaaattttt gaacaatttg atgatattag aagagttctt gcacatgcat ctgctattcc accagaatta attattgaaa gtttcagaaa attagaacca gaacaagcta ttcttgttct tcaagacctt ttaagaacaa atccaagagg aaatcttcaa attattatta aaatattact tgaattccat acaggacttg gagatgataa agtcattgat ttacttcaaa agtttgattg ttgggaagga ttattctatt tcttatctca aattgtgaat actactaatg acaaaatggt tgtatttaaa tatattgagg catgtgccag atcaggacaa ttccaagaat tagcacgtgt tgttcaagag aatgatgtat atgatccaaa agaagttaaa gaattcttaa agaatgcaaa aattgcagac caaattccat ttattattgt atgtgattaa gtgagta
Expected Protein Sequence
MAHHHHHHMK LPAVEAWIVN DKIECSEKAG DLVKPYDQRL ALMIYSKAHA TGKVVQSLAE MGQSDKIVAY CERAGFSPDY LQILQQILPA SGEAAKAYAA SLIQQKGYDE LDMISIIDLF ARYRYLKEIT ALILDTLDGD NEQYANIQTK VLEMNLIGAP QIADTIFEND MLKHFDHNRI GKLCEQAGLF KRALQIFEQF DDIRRVLAHA SAIPPELIIE SFRKLEPEQA ILVLQDLLRT NPRGNLQIII KILLEFHTGL GDDKVIDLLQ KFDCWEGLFY FLSQIVNTTN DKMVVFKYIE ACARSGQFQE LARVVQENDV YDPKEVKEFL KNAKIADQIP FIIVCD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac ttccagcagt tgaggcatgg attgtaaatg ataaaattga atgcagtgaa aaagcaggag atttagttaa gccatatgat caacgacttg cattaatgat ttattctaaa gcacatgcta caggaaaagt agttcaatca ttagcagaaa tgggacaaag tgataaaata gttgcttatt gtgaaagagc aggattttca ccagactatc ttcaaattct tcaacaaatt cttccagcat caggagaagc tgctaaggca tatgctgcaa gtttaattca acaaaaagga tatgatgaat tagatatgat ttcaataata gatctttttg ctagatatag atatcttaaa gaaattactg cattaatact tgatactctt gatggagata atgaacaata tgctaatatt caaacaaaag ttcttgaaat gaatttaatt ggagccccac aaattgctga tactattttt gagaatgata tgttaaaaca ttttgatcat aatagaattg gtaaattgtg tgaacaagct ggattattta aacgagctct tcaaattttt gaacaatttg atgatattag aagagttctt gcacatgcat ctgctattcc accagaatta attattgaaa gttttagaaa attagaacca gaacaagcta ttcttgttct tcaagacctt ttaagaacaa atccaagagg aaatcttcaa attattatta aaatattact tgaattccat acaggacttg gagatgataa agtcattgat ttacttcaaa agtttgattg ttgggaagga ttattctatt tcttatctca aattgtgaat actactaatg acaaaatggt tgtatttaaa tatattgagg catgtgccag atcaggacaa ttccaagaat tagcacgtgt tgttcaagag aatgatgtat atgatccaaa agaagttaaa gaattcttaa agaatgcaaa aattgcagac caaattccat ttattattgt atgtgattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for EnhiA.18428.a.B4.GE35751
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/3/2012
EXPECTED MW: 16kDa
OBSERVED MW: 14kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN HRFIEAYVKK INPMNTPKVV GALLDCGCGE DYIQQLINAV GGICPADELI KTCESRNRLI ILLPWLEQRL SEGSPDVSLH TALAKVYVDQ GKDAEKFLNN DMYYDSLIVG KYCEDRDPYL SYICYKRKNL DDE
Validated NT Sequence
aattttgttt actttaagaa ggagatatac catggctcac caccaccacc accatatgaa tcatagattt attgaggcat atgttaagaa aattaatcca atgaatacac caaaagttgt tggtgcatta ttagattgtg gatgtggaga agattatatt caacaattaa ttaatgcagt tggaggtatt tgtccagctg atgaattgat taagacttgt gaaagtagaa atagattaat tattttatta ccatggttag aacaaagatt atctgaagga tctcctgatg tttctcttca tacagctctt gctaaagttt atgttgacca aggaaaagat gcagagaaat tcttaaataa tgatatgtat tatgactcat taattgtagg taaatattgt gaagatagag atccatattt atcatacatt tgttataaga gaaagaattt ggatgatgaa taagtgagta agataggatc cggctgctaa caaagcccga aaga
Expected Protein Sequence
MAHHHHHHMN HRFIEAYVKK INPMNTPKVV GALLDCGCGE DYIQQLINAV GGICPADELI KTCESRNRLI ILLPWLEQRL SEGSPDVSLH TALAKVYVDQ GKDAEKFLNN DMYYDSLIVG KYCEDRDPYL SYICYKRKNL DDE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatc atagatttat tgaagcatat gttaagaaaa ttaatccaat gaatacacca aaagttgttg gtgcattatt agattgtgga tgtggagaag attatattca acaattaatt aatgcagttg gaggtatttg tccagctgat gaattgatta agacttgtga aagtagaaat agattaatta ttttattacc atggttagaa caaagattat ctgaaggatc tcctgatgtt tctcttcata cagctcttgc taaagtttat gttgaccaag gaaaagatgc agagaaattc ttaaataatg atatgtatta tgactcatta attgtaggta aatattgtga agatagagat ccatatttat catacatttg ttataagaga aagaatttgg atgatgaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for EnhiA.18428.a.B5.GE35752
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/6/2012
EXPECTED MW: 20kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHME KFLNNDMYYD SLIVGKYCED RDPYLSYICY KRKNLDDELI DVTNRHNLFK YQAKYLVERE DLDLWNKVLT EENEHRKSVV EQVIHTALPE CTDPNQILKT VQAFLQNDLP NDLIDLLDKL VSQNPEYSTN PTLQKLLIQT SIKAAPNRVM DYIRKLNDYD PKEIA
Validated NT Sequence
ataccatggc tcaccaccac caccaccata tggagaaatt cttaaataat gatatgtatt atgactcatt aattgtaggt aaatattgtg aagatagaga tccatattta tcatacattt gttataagag aaagaatttg gatgatgaat tgattgatgt aactaataga cataatttat tcaaatacca agcaaaatat cttgttgaaa gagaagatct tgatttatgg aataaagtct tgacagaaga aaatgaacat agaaagagtg ttgttgaaca agttattcat actgcacttc cagaatgtac tgatccaaat caaattctca aaacagttca agcattcctt caaaatgacc ttccaaatga tttgattgat ttattggata aattagtttc tcaaaatcca gaatatagca ctaatccaac attacaaaaa ttattgattc aaactagtat taaagcagca ccaaatagag ttatggatta tattagaaaa cttaatgact atgatccaaa agaaattgct taagtgagta agataggatc cgg
Expected Protein Sequence
MAHHHHHHME KFLNNDMYYD SLIVGKYCED RDPYLSYICY KRKNLDDELI DVTNRHNLFK YQAKYLVERE DLDLWNKVLT EENEHRKSVV EQVIHTALPE CTDPNQILKT VQAFLQNDLP NDLIDLLDKL VSQNPEYSTN PTLQKLLIQT SIKAAPNRVM DYIRKLNDYD PKEIA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaga aattcttaaa taatgatatg tattatgact cattaattgt aggtaaatat tgtgaagata gagatccata tttatcatac atttgttata agagaaagaa tttggatgat gaattgattg atgtaactaa tagacataat ttattcaaat accaagcaaa atatcttgtt gaaagagaag atcttgattt atggaataaa gtcttgacag aagaaaatga acatagaaag agtgttgttg aacaagttat tcatactgca cttccagaat gtactgatcc aaatcaaatt ctcaaaacag ttcaagcatt ccttcaaaat gaccttccaa atgatttgat tgatttattg gataaattag tttctcaaaa tccagaatat agcactaatc caacattaca aaaattattg attcaaacta gtattaaagc agcaccaaat agagttatgg attatattag aaaacttaat gactatgatc caaaagaaat tgcttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for EnhiA.18428.a.B6.GE35753
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/3/2012
EXPECTED MW: 18kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK EIAMDVLLQH IKDLTRAKDF ADKAKKDILY KKLGEAQLDE LKVKDAIISL MKAKDISLRS RVIDIAESDG SYEDLINYLL MCKEETKDMM VETELLYCYA KLKKNDEIEN FLKTANCANL TSIAERCYNE ELYGAAKILY TSLNNYIK
Validated NT Sequence
tattttgttt actttaagaa ggagatatac catggctcac caccaccacc accatatgaa agaaattgct atggatgtat tattacaaca cattaaagac cttactcgtg ctaaagactt tgctgataaa gctaaaaaag atattttata taagaaactt ggagaagcac aacttgatga attgaaagtt aaagacgcta ttatttcatt gatgaaagct aaggacatta gtttaagatc aagagttatt gatattgcag agtcagatgg atcatatgaa gatttaatta attacttact tatgtgtaag gaagaaacta aagacatgat ggttgaaact gaattattat attgttatgc taaattgaaa aagaatgatg aaattgaaaa cttccttaaa acagctaatt gtgcaaatct tacctccatt gctgaaagat gttataatga agaattatat ggagctgcta agattcttta tacatcatta aataattata ttaaataagt gagtaagata ggatccggct gctaannnnn ncccgaaaga nnantnnnnn nnnnnnannn nnnntntcnn cnnannntnn ntntnttnnn nnntnttcnn nttnnntcnn nt
Expected Protein Sequence
MAHHHHHHMK EIAMDVLLQH IKDLTRAKDF ADKAKKDILY KKLGEAQLDE LKVKDAIISL MKAKDISLRS RVIDIAESDG SYEDLINYLL MCKEETKDMM VETELLYCYA KLKKNDEIEN FLKTANCANL TSIAERCYNE ELYGAAKILY TSLNNYIK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag aaattgctat ggatgtatta ttacaacaca ttaaagacct tactcgtgct aaagactttg ctgataaagc taaaaaagat attttatata agaaacttgg agaagcacaa cttgatgaat tgaaagttaa agacgctatt atttcattga tgaaagctaa ggacattagt ttaagatcaa gagttattga tattgcagag tcagatggat catatgaaga tttaattaat tacttactta tgtgtaagga agaaactaaa gacatgatgg ttgaaactga attattatat tgttatgcta aattgaaaaa gaatgatgaa attgaaaact tccttaaaac agctaattgt gcaaatctta cctccattgc tgaaagatgt tataatgaag aattatatgg agctgctaag attctttata catcattaaa taattatatt aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for EnhiA.18428.a.B7.GE35754
HARVESTED ON: 7/31/2012
SEQUENCED ON: 10/23/2013
EXPECTED MW: 39kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK NDEIENFLKT ANCANLTSIA ERCYNEELYG AAKILYTSLN NYIKLASCLL KLKDYAGAVE AAKKANSTRT WKEVTFACID AKEFTLAQET GINILMAGDE ITELVYYYEK NELYDQVIEL LEAGLKIENV HVSMFTELAI LYSKYKEEKL YDYLKQYVAK IQCQKVIPTV NMNQQWKELV FLYVQVDQVK AIETMISYPD DCFDHQLMKE LLVNVPRIDM IYKAESYYLA EKPEKVNEML IAVAHRCDHT QVISIARKEK DLKTIREYLL YCLDLNNDSV NEALIDLFID DGDAKSLKSL IEKNTNFNKT GLANRLKVHE DVEFRKIAAY LYSR
Validated NT Sequence
atggctcacc accaccacca ccatatgaag aatgatgaaa ttgaaaactt ccttaaaaca gctaattgtg caaatcttac ctccattgct gaaagatgtt ataatgaaga attatatgga gctgctaaga ttctttatac atcattaaat aattatatta aattagcatc atgcttactt aaattaaagg attatgctgg agctgtagaa gctgctaaaa aggctaatag tactagaaca tggaaagaag ttacatttgc atgtattgat gcaaaagaat ttacattagc acaagaaaca ggaattaata tcttaatggc tggtgatgaa attactgaat tagtttatta ttatgaaaag aatgaattat atgatcaagt cattgaatta cttgaagcag gattaaagat tgaaaatgtt catgtatcta tgtttactga attagctatt ctttattcta aatataaaga agaaaagtta tatgattatc ttaaacaata tgttgctaag attcaatgcc aaaaagttat tccaaccgtc aatatgaatc aacaatggaa agaacttgta ttcctttatg ttcaagttga tcaagttaaa gctattgaga ctatgatttc atatccagat gattgtttcg atcatcaatt aatgaaagaa ttattagtta atgttccaag aattgatatg atttataaag ctgaatcata ttatcttgca gaaaaaccag aaaaggttaa tgaaatgtta attgctgtag ctcatagatg tgaccatact caagttattt ctattgctcg taaagaaaaa gatcttaaaa ctattagaga atatcttctt tattgtcttg atttaaataa tgattcagtc aatgaagcac ttattgatct tttcattgat gatggagatg ctaagagctt aaagagtctt atcgaaaaga atacaaactt caacaaaact ggattggcta atagattgaa agttcatgaa gatgttgaat ttagaaaaat tgcagcatat ctttatagta gataagtnag ta
Expected Protein Sequence
MAHHHHHHMK NDEIENFLKT ANCANLTSIA ERCYNEELYG AAKILYTSLN NYIKLASCLL KLKDYAGAVE AAKKANSTRT WKEVTFACID AKEFTLAQET GINILMAGDE ITELVYYYEK NELYDQVIEL LEAGLKIENV HVSMFTELAI LYSKYKEEKL YDYLKQYVAK IQCQKVIPTV NMNQQWKELV FLYVQVDQVK AIETMISYPD DCFDHQLMKE LLVNVPRIDM IYKAESYYLA EKPEKVNEML IAVAHRCDHT QVISIARKEK DLKTIREYLL YCLDLNNDSV NEALIDLFID DGDAKSLKSL IEKNTNFNKT GLANRLKVHE DVEFRKIAAY LYSR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga atgatgaaat tgaaaacttc cttaaaacag ctaattgtgc aaatcttacc tccattgctg aaagatgtta taatgaagaa ttatatggag ctgctaagat tctttataca tcattaaata attatattaa attagcatca tgcttactta aattaaagga ttatgctgga gctgtagaag ctgctaaaaa ggctaatagt actagaacat ggaaagaagt tacatttgca tgtattgatg caaaagaatt tacattagca caagaaacag gaattaatat cttaatggct ggtgatgaaa ttactgaatt agtttattat tatgaaaaga atgaattata tgatcaagtc attgaattac ttgaagcagg attaaagatt gaaaatgttc atgtatctat gtttactgaa ttagctattc tttattctaa atataaagaa gaaaagttat atgattatct taaacaatat gttgctaaga ttcaatgcca aaaagttatt ccaaccgtca atatgaatca acaatggaaa gaacttgtat tcctttatgt tcaagttgat caagttaaag ctattgagac tatgatttca tatccagatg attgtttcga tcatcaatta atgaaagaat tattagttaa tgttccaaga attgatatga tttataaagc tgaatcatat tatcttgcag aaaaaccaga aaaggttaat gaaatgttaa ttgctgtagc tcatagatgt gaccatactc aagttatttc tattgctcgt aaagaaaaag atcttaaaac tattagagaa tatcttcttt attgtcttga tttaaataat gattcagtca atgaagcact tattgatctt ttcattgatg atggagatgc taagagctta aagagtctta tcgaaaagaa tacaaacttc aacaaaactg gattggctaa tagattgaaa gttcatgaag atgttgaatt tagaaaaatt gcagcatatc tttatagtag atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for EnhiA.18428.a.B3.PS01634
PURIFICATION DATe: 9/27/2012
CONCENTRATION: 7.5mg/ml
OBSERVED MW: 31kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MKLPAVEAWI VNDKIECSEK AGDLVKPYDQ RLALMIYSKA HATGKVVQSL AEMGQSDKIV AYCERAGFSP DYLQILQQIL PASGEAAKAY AASLIQQKGY DELDMISIID LFARYRYLKE ITALILDTLD GDNEQYANIQ TKVLEMNLIG APQIADTIFE NDMLKHFDHN RIGKLCEQAG LFKRALQIFE QFDDIRRVLA HASAIPPELI IESFRKLEPE QAILVLQDLL RTNPRGNLQI IIKILLEFHT GLGDDKVIDL LQKFDCWEGL FYFLSQIVNT TNDKMVVFKY IEACARSGQF QELARVVQEN DVYDPKEVKE FLKNAKIADQ IPFIIVCD
Validated NT Sequence
ccaccaccac catatgaaac ttccagcagt tgaggcatgg attgtaaatg ataaaattga atgcagtgaa aaagcaggag atttagttaa gccatatgat caacgacttg cattaatgat ttattctaaa gcacatgcta caggaaaagt agttcaatca ttagcagaaa tgggacaaag tgataaaata gttgcttatt gtgaaagagc aggattttca ccagactatc ttcaaattct tcaacaaatt cttccagcat caggagaagc tgctaaggca tatgctgcaa gtttaattca acaaaaagga tatgatgaat tagatatgat ttcaataata gatctttttg ctagatatag atatcttaaa gaaattactg cattaatact tgatactctt gatggagata atgaacaata tgctaatatt caaacaaaag ttcttgaaat gaatttaatt ggagccccac aaattgctga tactattttt gagaatgata tgttaaaaca ttttgatcat aatagaattg gtaaattgtg tgaacaagct ggattattta aacgagctct tcaaattttt gaacaatttg atgatattag aagagttctt gcacatgcat ctgctattcc accagaatta attattgaaa gtttcagaaa attagaacca gaacaagcta ttcttgttct tcaagacctt ttaagaacaa atccaagagg aaatcttcaa attattatta aaatattact tgaattccat acaggacttg gagatgataa agtcattgat ttacttcaaa agtttgattg ttgggaagga ttattctatt tcttatctca aattgtgaat actactaatg acaaaatggt tgtatttaaa tatattgagg catgtgccag atcaggacaa ttccaagaat tagcacgtgt tgttcaagag aatgatgtat atgatccaaa agaagttaaa gaattcttaa agaatgcaaa aattgcagac caaattccat ttattattgt atgtgattaa gtgagta
Expressed Protein Sequence
MAHHHHHHMK LPAVEAWIVN DKIECSEKAG DLVKPYDQRL ALMIYSKAHA TGKVVQSLAE MGQSDKIVAY CERAGFSPDY LQILQQILPA SGEAAKAYAA SLIQQKGYDE LDMISIIDLF ARYRYLKEIT ALILDTLDGD NEQYANIQTK VLEMNLIGAP QIADTIFEND MLKHFDHNRI GKLCEQAGLF KRALQIFEQF DDIRRVLAHA SAIPPELIIE SFRKLEPEQA ILVLQDLLRT NPRGNLQIII KILLEFHTGL GDDKVIDLLQ KFDCWEGLFY FLSQIVNTTN DKMVVFKYIE ACARSGQFQE LARVVQENDV YDPKEVKEFL KNAKIADQIP FIIVCD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAC TTCCAGCAGT TGAGGCATGG ATTGTAAATG ATAAAATTGA ATGCAGTGAA AAAGCAGGAG ATTTAGTTAA GCCATATGAT CAACGACTTG CATTAATGAT TTATTCTAAA GCACATGCTA CAGGAAAAGT AGTTCAATCA TTAGCAGAAA TGGGACAAAG TGATAAAATA GTTGCTTATT GTGAAAGAGC AGGATTTTCA CCAGACTATC TTCAAATTCT TCAACAAATT CTTCCAGCAT CAGGAGAAGC TGCTAAGGCA TATGCTGCAA GTTTAATTCA ACAAAAAGGA TATGATGAAT TAGATATGAT TTCAATAATA GATCTTTTTG CTAGATATAG ATATCTTAAA GAAATTACTG CATTAATACT TGATACTCTT GATGGAGATA ATGAACAATA TGCTAATATT CAAACAAAAG TTCTTGAAAT GAATTTAATT GGAGCCCCAC AAATTGCTGA TACTATTTTT GAGAATGATA TGTTAAAACA TTTTGATCAT AATAGAATTG GTAAATTGTG TGAACAAGCT GGATTATTTA AACGAGCTCT TCAAATTTTT GAACAATTTG ATGATATTAG AAGAGTTCTT GCACATGCAT CTGCTATTCC ACCAGAATTA ATTATTGAAA GTTTTAGAAA ATTAGAACCA GAACAAGCTA TTCTTGTTCT TCAAGACCTT TTAAGAACAA ATCCAAGAGG AAATCTTCAA ATTATTATTA AAATATTACT TGAATTCCAT ACAGGACTTG GAGATGATAA AGTCATTGAT TTACTTCAAA AGTTTGATTG TTGGGAAGGA TTATTCTATT TCTTATCTCA AATTGTGAAT ACTACTAATG ACAAAATGGT TGTATTTAAA TATATTGAGG CATGTGCCAG ATCAGGACAA TTCCAAGAAT TAGCACGTGT TGTTCAAGAG AATGATGTAT ATGATCCAAA AGAAGTTAAA GAATTCTTAA AGAATGCAAA AATTGCAGAC CAAATTCCAT TTATTATTGT ATGTGATTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT