EnhiA.18396.a

JAB1; uncharacterized protein

CENTER ID: EnhiA.18396.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.18396.a.B1.GE42746 full length 1 318
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_050500
UniProt: C4LTS3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGEEAAYKEW EKVNGVKYIE EDKLLEWNDS EREQIFKDRP WKKDPYYFKK CYVSSVALLK MVMHAKQGEP LEIMGILIGQ TKGDSFVITD VVSLPVEGTE TRVNASADCD AYMLQYGEYK NSTGFKEPFC GWYHSHPSYK CWLSGIDVAT EKLHQSINDP WIAIVVDPVT TSTNGKIEIG AFRTFPEGFK PQQKAEMKKV LPSEKIADFG SYYDSYYSIK VELFKTKLDD QVLRLLWHEY WINTLAATAI ISSRDVMDEK IIDLYDKFTA ELKNNKSNVV DACGAILDDA KEIQMIYERG IKSLDLKNIL FNQKVTGK
NT Sequence
atgggagaag aagctgcata taaagaatgg gaaaaagtaa atggagtaaa atacattgaa gaagataagt tattagaatg gaatgattct gaacgagaac aaatttttaa agatagacca tggaagaaag atccatatta tttcaaaaaa tgttatgtta gttcagtggc attattaaaa atggtaatgc acgcaaaaca aggagagcca ttagagatta tgggtattct tattggacaa acaaaagggg attcatttgt gattactgat gtagttagtc ttccagttga aggaactgaa acaagagtta atgcatctgc tgattgtgat gcttatatgt tacaatatgg agagtacaaa aatagtactg gatttaaaga acctttttgt ggatggtatc actcacatcc aagttataaa tgttggttat ctggtattga tgttgctaca gaaaaattac accaaagcat taacgatcca tggattgcta tagttgtaga tcctgtcaca acatctacaa atggtaaaat agaaattggt gcatttagaa cattcccaga aggatttaaa ccacaacaaa aagctgaaat gaaaaaagta cttccaagtg agaaaatagc agattttggg tcgtattatg attcatatta ttctattaaa gttgaattat ttaaaacaaa attagatgac caagtacttc gtttattatg gcatgaatat tggattaaca ctcttgctgc aacagctatt atttcttcac gagatgttat ggatgaaaaa attattgact tatatgataa atttactgcg gaattaaaaa ataataaaag taatgttgta gatgcttgtg gtgctatttt agatgatgca aaagaaatcc aaatgattta tgaacgaggt attaaatctt tagatttaaa gaatatttta tttaatcaga aagttactgg aaaataa
Details for EnhiA.18396.a.B1.GE42746
HARVESTED ON: 11/5/2018
SEQUENCED ON: 11/7/2018
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMG EEAAYKEWEK VNGVKYIEED KLLEWNDSER EQIFKDRPWK KDPYYFKKCY VSSVALLKMV MHAKQGEPLE IMGILIGQTK GDSFVITDVV SLPVEGTETR VNASADCDAY MLQYGEYKNS TGFKEPFCGW YHSHPSYKCW LSGIDVATEK LHQSINDPWI AIVVDPVTTS TNGKIEIGAF RTFPEGFKPQ QKAEMKKVLP SEKIADFGSY YDSYYSIKVE LFKTKLDDQV LRLLWHEYWI NTLAATAIIS SRDVMDEKII DLYDKFTAEL KNNKSNVVDA CGAILDDAKE IQMIYERGIK SLDLKNI
Validated NT Sequence
aaaatattct ttaaatctaa agatttaata cctcgttcat aaatcatttg gatttctttt gcatcatcta aaatagcacc acaagcatct acaacattac ttttattatt ttttaattcc gcagtaaatt tatcatataa gtcaataatt ttttcatcca taacatctcg tgaagaaata atagctgttg cagcaagagt gttaatccaa tattcatgcc ataataaacg aagtacttgg tcatctaatt ttgttttaaa taattcaact ttaatagaat aatatgaatc ataatacgac ccaaaatctg ctattttctc acttggaagt acttttttca tttcagcttt ttgttgtggt ttaaatcctt ctgggaatgt tctaaatgca ccaatttcta ttttaccatt tgtagatgtt gtgacaggat ctacaactat agcaatccat ggatcgttaa tgctttggtg taatttttct gtagcaacat caataccaga taaccaacat ttataacttg gatgtgagtg ataccatcca caaaaaggtt ctttaaatcc agtactattt ttgtactctc catattgtaa catataagca tcacaatcag cagatgcatt aactcttgtt tcagttcctt caactggaag actaactaca tcagtaatca caaatgaatc cccttttgtt tgtccaataa gaatacccat aatctctaat ggctctcctt gttttgcgtg cattaccatt tttaataatg ccactgaact aacataacat tttttgaaat aatatggatc tttcttccat ggtctatctt taaaaatttg ttctcgttca gaatcattcc attctaataa cttatcttct tcaatgtatt ttactccatt tactttttcc cattctttat atgcagcttc ttctcccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMG EEAAYKEWEK VNGVKYIEED KLLEWNDSER EQIFKDRPWK KDPYYFKKCY VSSVALLKMV MHAKQGEPLE IMGILIGQTK GDSFVITDVV SLPVEGTETR VNASADCDAY MLQYGEYKNS TGFKEPFCGW YHSHPSYKCW LSGIDVATEK LHQSINDPWI AIVVDPVTTS TNGKIEIGAF RTFPEGFKPQ QKAEMKKVLP SEKIADFGSY YDSYYSIKVE LFKTKLDDQV LRLLWHEYWI NTLAATAIIS SRDVMDEKII DLYDKFTAEL KNNKSNVVDA CGAILDDAKE IQMIYERGIK SLDLKNILFN QKVTGK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggag aagaagctgc atataaagaa tgggaaaaag taaatggagt aaaatacatt gaagaagata agttattaga atggaatgat tctgaacgag aacaaatttt taaagataga ccatggaaga aagatccata ttatttcaaa aaatgttatg ttagttcagt ggcattatta aaaatggtaa tgcacgcaaa acaaggagag ccattagaga ttatgggtat tcttattgga caaacaaaag gggattcatt tgtgattact gatgtagtta gtcttccagt tgaaggaact gaaacaagag ttaatgcatc tgctgattgt gatgcttata tgttacaata tggagagtac aaaaatagta ctggatttaa agaacctttt tgtggatggt atcactcaca tccaagttat aaatgttggt tatctggtat tgatgttgct acagaaaaat tacaccaaag cattaacgat ccatggattg ctatagttgt agatcctgtc acaacatcta caaatggtaa aatagaaatt ggtgcattta gaacattccc agaaggattt aaaccacaac aaaaagctga aatgaaaaaa gtacttccaa gtgagaaaat agcagatttt gggtcgtatt atgattcata ttattctatt aaagttgaat tatttaaaac aaaattagat gaccaagtac ttcgtttatt atggcatgaa tattggatta acactcttgc tgcaacagct attatttctt cacgagatgt tatggatgaa aaaattattg acttatatga taaatttact gcggaattaa aaaataataa aagtaatgtt gtagatgctt gtggtgctat tttagatgat gcaaaagaaa tccaaatgat ttatgaacga ggtattaaat ctttagattt aaagaatatt ttatttaatc agaaagttac tggaaaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at